Abstract：
:Immunological detection has been developed into a sensitive and versatile technique and is based on two requisite elements: targeting antibodies and an indicator, usually in the form of horseradish peroxidase or alkaline phosphatase. The specificity and turnover rate of these enzymes provide an efficient means of signal amplification, but both require a stopping agent to prevent overdevelopment, which limits scalability to mechanized fluidic systems. As an alternative, we present a fully optical detection system based on a streptavidin-singlet oxygen generating chimeric protein that produces singlet oxygen from blue light. When used with trans-1-(2'-methoxyvinyl)pyrene, the photosynthetic streptavidin combines indicator development and reporting in sufficiently distinct visible wavelengths while retaining the sensitivity and scale of enzymatic systems that use horseradish peroxidase. By combining photosensitive development and detection into one system, we can enable future, highly parallel immunological testing to be controlled with the spatial and temporal precision of light.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Wurtzler EM,Wendell Ddoi
10.1021/bm401511asubject
Has Abstractpub_date
2014-01-13 00:00:00pages
228-33issue
1eissn
1525-7797issn
1526-4602journal_volume
15pub_type
杂志文章abstract：:Self-assembled hydrogen-bonded networks of the polysaccharide pectin, a mechanically functional component of plant cell walls, have been of recent interest as biomimetic exemplars of physical gels, and the microrheological and strain-stiffening behaviors have been previously investigated. Despite this detailed rheolog...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00870
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journal_title：Biomacromolecules
pub_type： 杂志文章
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2012-06-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2006-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301289n
更新日期：2012-12-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2010-08-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2015-07-13 00:00:00
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journal_title：Biomacromolecules
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更新日期：2012-04-09 00:00:00
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journal_title：Biomacromolecules
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更新日期：2014-05-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2015-07-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00582
更新日期：2016-07-11 00:00:00
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journal_title：Biomacromolecules
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更新日期：2005-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01209
更新日期：2017-12-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2017-10-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401309u
更新日期：2014-01-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900889b
更新日期：2010-01-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800321w
更新日期：2008-08-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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journal_title：Biomacromolecules
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更新日期：2005-09-01 00:00:00
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
pub_type： 杂志文章,评审
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更新日期：2019-04-08 00:00:00
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journal_title：Biomacromolecules
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更新日期：2001-10-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2020-02-10 00:00:00
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journal_title：Biomacromolecules
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更新日期：2004-07-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2017-01-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2012-05-14 00:00:00
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journal_title：Biomacromolecules
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更新日期：2005-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2012-12-10 00:00:00
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