Abstract：
:Biodegradable multilayered capsules encapsulating basic fibroblast growth factor (bFGF) were developed as a cytokine release carrier for drug delivery systems. The multilayered hollow capsules were fabricated via the layer-by-layer (LbL) assembly of chitosan (CT) and dextran sulfate (Dex). The bFGF was encapsulated into the CT/Dex multilayered capsules by controlling the membrane permeability, and the local and sustained release of bFGF from the capsules was examined. At pH < 8.0, the capsule membrane tightened, and FITC-dextran ( Mw = 4000) could not enter the capsules. However, FITC-dextran ( M w = 250000) easily entered the capsules at pH > 8.0, which can be attributed to the electrostatic repulsion of Dex caused by the deprotonation of the amine group in CT. After treatment with acetic acid buffer (pH 5.6), FITC-dextran or bFGF was successfully encapsulated into the capsules. The amount of encapsulated bFGF was approximately 34 microg/1 mg of capsule. Initially, about 30% of the encapsulated bFGF was released in serum-free medium within a few hours, however, the release was sustained over 70 h. When the bFGF encapsulating capsules were added to cell culture medium (serum-free), the mouse L929 fibroblast cells proliferated well for 2 weeks as compared to cultures, where bFGF was added to the medium or where bFGF and empty hollow capsules were added separately. The proliferation is due to the local and sustained release of bFGF from the adsorbent capsule to the cell surface.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Itoh Y,Matsusaki M,Kida T,Akashi Mdoi
10.1021/bm800321wsubject
Has Abstractpub_date
2008-08-01 00:00:00pages
2202-6issue
8eissn
1525-7797issn
1526-4602journal_volume
9pub_type
杂志文章abstract：:Biocompatible photoresponsive materials are of interest for targeted drug delivery, tissue engineering, 2D and 3D protein patterning, and other biomedical applications. We prepared light degradable hydrogels using a natural alginate polysaccharide cross-linked with iron(III) cations. The "hard" iron(III) cations used ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300707a
更新日期：2012-08-13 00:00:00
abstract：:Our previous studies have demonstrated the efficacy of generation 5 poly(amidoamine) dendrimers (G5-PAMAM) as a platform for the targeted delivery of chemotherapeutics. However, anticancer therapy can be subverted by anti-apoptotic changes in cancer cells. Bcl-2 and several of its peptides are commonly overexpressed i...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700727g
更新日期：2007-10-01 00:00:00
abstract：:Mixed protein-based hydrogels have been prepared by blending gelatin (G) with amorphous Bombyx mori silk fibroin (SF) and promoting beta-crystallization of SF via subsequent exposure to methanol or methanol/water solutions. The introduction of beta crystals in SF serves to stabilize the hydrogel network and extend the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050396c
更新日期：2005-11-01 00:00:00
abstract：:The polarity of polymer-actin complexes obtained by mixing F-actin with synthetic polymers carrying positive charges such as poly(L-lysine), x,y-ionene bromide polymers, and poly(N-[3-(dimethylamino)propyl]acrylamide) (PDMAPAA-Q) have been investigated. Actin complexes formed with poly(L-lysine) and PDMAPAA-Q, which c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0494038
更新日期：2005-03-01 00:00:00
abstract：:Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801430x
更新日期：2009-05-11 00:00:00
abstract：:We present a synthetic approach toward soft, glycooligomer-functionalized microgel particles mimicking carbohydrate presenting cell surfaces and analyze their specific binding to a model lectin (Concanavalin A, ConA). Focusing on multivalent presentation, a series of sequence-controlled glycooligomers with varying spa...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00790
更新日期：2018-08-13 00:00:00
abstract：:G-quadruplex is a type of DNA secondary structure formed by specific guanine-rich sequences. Because of their enrichment in functional genomic regions and their biological significance, G-quadruplexes are recognized as significant drug targets for cancer and other diseases. Here, we tested the precipitation efficiency...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100862k
更新日期：2010-12-13 00:00:00
abstract：:This paper reports the synthesis of hyaluronan (HA) and its derivatives via the hyaluronidase-catalyzed polymerization of 2-substituted oxazoline derivative monomers designed as "transition-state analogue substrates". Polymerization of 2-methyl oxazoline monomer from N-acetylhyalobiuronate (GlcAbeta(1-->3)GlcNAc) effe...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049280r
更新日期：2005-03-01 00:00:00
abstract：:Silicatein α exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly β-sheet structure. However, it is produced in a soluble form with mixed α-helix/β-sheet structure akin to its cathepsin L homologue. To understand this conformational transition in the context of...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100932e
更新日期：2010-11-08 00:00:00
abstract：:A series of chitosan/chitin samples with DA's varying between 5.2 and 89% was prepared from the reacetylation under soft conditions of a unique chitosan sample allowing the preservation of the chain distribution. The study of the variation of pH for the same concentration of amine groups, at different ionic strengths,...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015531+
更新日期：2001-10-01 00:00:00
abstract：:Gastric mucin, a high molecular weight glycoprotein, is responsible for providing the gel-forming properties and protective function of the gastric mucus layer. Bulk rheology measurements in the linear viscoelastic regime show that gastric mucin undergoes a pH-dependent sol-gel transition from a viscoelastic solution ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0609691
更新日期：2007-05-01 00:00:00
abstract：:A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based on three different core sizes (18, 35, 65 nm) and five polymer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300248u
更新日期：2012-05-14 00:00:00
abstract：:Binding interactions between low molecular weight heparin (LMWH) and heparin-binding peptides (HBP) have been applied as a strategy for the assembly of hydrogels that are capable of sequestering growth factors and delivering them in a controlled manner. In this work, the assembly of four-arm star poly(ethylene glycol)...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0500042
更新日期：2005-07-01 00:00:00
abstract：:Single-molecule total internal reflection fluorescence microscopy was used to observe the dynamic behavior of polycytosine single-stranded DNA (ssDNA) (1-50 nucleotides long) at the interface between aqueous solution and hydrophilic (oligoethylene glycol-modified fused silica, OEG) and hydrophobic (octadecyltriethoxys...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301289n
更新日期：2012-12-10 00:00:00
abstract：:Limited studies have been done on the interactions between subunits of self-assembling protein cages. E2 protein cage from Bacillus stearothermophilus was investigated in this work to impart pH-sensitive disassembly profile. Key amino acids were identified at the intratrimer and intertrimer interfaces, and histidine r...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2005438
更新日期：2011-09-12 00:00:00
abstract：:Decomposable hollow capsules based on deoxyribonucleic acid (DNA) and a low molecular weight organic molecule, a naturally occurring polyamine, spermidine (SP), were formed by applying the layer-by-layer adsorption strategy to colloid particles, viz., assembling DNA/SP multilayers on colloids and subsequently removing...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010052w
更新日期：2001-10-01 00:00:00
abstract：:Well-defined linear poly(acryloyl glucosamine) (PAGA) exhibiting molar masses ranging from 3 to 120 K and low polydispersities have been prepared via reversible addition-fragmentation chain transfer polymerization (RAFT) in aqueous solution without recourse to protecting group chemistry. The livingness of the process ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0506086
更新日期：2006-01-01 00:00:00
abstract：:This paper reports the rheological behavior of chitosan solutions that have been cross-linked with different amounts of genipin, at body temperature and physiological pH. The effect of the cross-linker loading on the rheological properties of hydrogels has been evaluated. The oscillatory time sweep method was used to ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700762w
更新日期：2007-12-01 00:00:00
abstract：:Spiders spin their silk from an aqueous solution to a solid fiber in ambient conditions. However, to date, the assembly mechanism in the spider silk gland has not been satisfactorily explained. In this paper, we use molecular dynamics simulations to model Nephila clavipes MaSp1 dragline silk formation under shear flow...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01246
更新日期：2016-02-08 00:00:00
abstract：:n-Heptyl α-d-mannose (HM) is a nanomolar antagonist of FimH, a virulence factor of E. coli. Herein we report on the construction of multivalent HM-based glycopolymers as potent antiadhesives of type 1 piliated E. coli. We investigate glycopolymer/FimH and glycopolymer/bacteria interactions and show that HM-based glyco...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00413
更新日期：2015-06-08 00:00:00
abstract：:A wool fiber sample was submitted to chemical-free steam explosion in view of potential exploitation of keratin-based industrial and farm wastes. Fiber keratin was converted into a dark-yellow sludge that was submitted to phase separation by filtration, centrifugation, and precipitation of the soluble materials from t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060597w
更新日期：2006-12-01 00:00:00
abstract：:The ability of small interfering RNA (siRNA) to efficiently silence the expression of specific genes provides the basis for exciting new therapies based on RNA interference (RNAi). The efficient intracellular delivery of siRNA from cell uptake through the endosomal trafficking pathways into the cytoplasm remains a sig...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100652w
更新日期：2010-11-08 00:00:00
abstract：:A variety of natural polymers and proteins are considered to be 3D cell culture structures able to mimic the extracellular matrix (ECM) to promote bone tissue regeneration. Pectin, a natural polysaccharide extracted from the plant cell walls and having a chemical structure similar to alginate, provides interesting pro...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101110x
更新日期：2011-03-14 00:00:00
abstract：:Thermoresponsive oligo(ethylene glycol)-based copolymers were investigated for trypsin conjugation. These copolymers have been synthesized by atom transfer radical polymerization of 2-(2-methoxyethoxy)ethyl methacrylate (MEO(2)MA) with oligo(ethylene glycol) methyl ether methacrylate (OEGMA(475), M(n) = 475 g.mol(-1))...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1005036
更新日期：2010-08-09 00:00:00
abstract：:The nature of the hydration of proteins and the subsequent implications for functionality is a matter of importance in both pharmaceutical and food applications. Most published studies rely on the use of one technique and attempt to characterize the system. Few studies have used combinations of techniques. In this pap...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm061206g
更新日期：2007-05-01 00:00:00
abstract：:We described the preparation of the glycol chitosan/heparin immobilized iron oxide nanoparticles (composite NPs) as a magnetic resonance imaging agent with a tumor-targeting characteristic. The iron oxide nanoseeds used clinically as a magnetic resonance imaging agent were immobilized into the glycol chitosan/heparin ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200413a
更新日期：2011-06-13 00:00:00
abstract：:Cartilage tissue engineering highly relies on the ability to store and transport chondrocytes in order to be clinically successful. Cryopreservation is a most reliable technology for chondrocyte storage, but it suffers from the intrinsic toxicity of current state-of-the-art cryoprotectant, dimethyl sulfoxide (DMSO). I...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01024
更新日期：2019-10-14 00:00:00
abstract：:Noncontact mode atomic force microscopy was used to investigate native silk proteins prepared in different ways. Low protein concentrations revealed that single protein molecules exhibit a simple, round shape with apparent diameters of 20-25 nm. Shearing the native protein solutions after extraction from the gland and...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201509b
更新日期：2012-03-12 00:00:00
abstract：:This study investigated the impact of the protein adsorption mechanism(s) on the efficiency of thermally controlled cell adhesion and release from poly(N-isopropyl acrylamide) brushes. Large format polymer gradients were used to screen for grafting densities and substrate chemistries that alter both cell adhesion at 3...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301390q
更新日期：2013-01-14 00:00:00
abstract：:Incorporating charged amino acid side chains in polypeptide polymer backbones to improve solubility usually leads to reduced secondary structuring. Here we show that highly water soluble (>15 mg.mL-1) β-sheets can be obtained via nucleotide monophosphate grafting onto simple poly(γ-propargyl- L-glutamate) backbone. Th...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01084
更新日期：2018-10-08 00:00:00
