Abstract：
:Single-molecule total internal reflection fluorescence microscopy was used to observe the dynamic behavior of polycytosine single-stranded DNA (ssDNA) (1-50 nucleotides long) at the interface between aqueous solution and hydrophilic (oligoethylene glycol-modified fused silica, OEG) and hydrophobic (octadecyltriethoxysilane-modified fused silica, OTES) solid surfaces. High throughput molecular tracking was used to determine >75,000 molecular trajectories for each molecular length, which were then used to calculate surface residence time and squared displacement (i.e., "step-size") distributions. On hydrophilic OEG surfaces, the surface residence time increased systematically with ssDNA chain length, as expected due to increasing molecule-surface interactions. Interestingly, the residence time decreased with increasing ssDNA length on the hydrophobic OTES surface, particularly for longer chains. Similarly, the interfacial mobility of polynucleotides slowed with increasing chain length on OEG, but became faster on OTES. On OTES surfaces, the rates associated with desorption and surface diffusion exhibited the distinctive anomalous temperature dependence that is characteristic of hydrophobic interactions for short-chain species but not for longer chains. These combined observations suggest that long oligonucleotides adopt conformations minimizing hydrophobic interactions, e.g., by internal sequestration of hydrophobic nucleobases.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Monserud JH,Schwartz DKdoi
10.1021/bm301289nsubject
Has Abstractpub_date
2012-12-10 00:00:00pages
4002-11issue
12eissn
1525-7797issn
1526-4602journal_volume
13pub_type
杂志文章abstract：:The carboxyl-terminal domain (CTD, residues 146-231) of the HIV-1 capsid (CA) protein plays an important role in the CA-CA dimerization and viral assembly of the human immunodeficiency virus type 1. Disrupting the native conformation of the CA is essential for blocking viral capsid formation and viral replication. Thu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801151r
更新日期：2009-02-09 00:00:00
abstract：:Hydrogel fibers that possessed a cell-adhesive surface and were degradable via enzymatic reactions were developed for fabricating tubular constructs with smooth muscle cell (SMC) and endothelial cell (EC) layers, similar to native blood vessels, in collagen gels. The fibers were prepared by soaking hydrogel fibers pre...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800286v
更新日期：2008-07-01 00:00:00
abstract：:Receptor-mediated, cell-specific delivery of siRNA enables silencing of target genes in specific tissues, opening the door to powerful therapeutic options for a multitude of diseases. However, the development of delivery systems capable of targeted and effective siRNA delivery typically requires multiple steps and the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200485b
更新日期：2011-07-11 00:00:00
abstract：:Articular cartilage defects, caused by trauma, osteoarthritis, or other diseases, always lead to severe joint pain and joint dysfunction. Without access to progenitor cells and the supply of blood and nutrients, the impaired articular cartilage would be short of the capability to self-repair. Although the present clin...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.9b00043
更新日期：2019-04-08 00:00:00
abstract：:Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modificati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00470
更新日期：2015-07-13 00:00:00
abstract：:Polycaprolactone (PCL) was reported a long time ago; however, its biomedical applications has not been extensively investigated in comparison with poly(lactide- co-glycolide) (PLGA) due to its too slow degradation profile. Here, we are reporting an oxalate-connected oligocaprolactone multiblock copolymer (PCL-OX) as a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00266
更新日期：2018-06-11 00:00:00
abstract：:Functionally designed elastomeric nanofiber fabrics made of the equimolar copolyester, poly(L-lactide-co-epsilon-caprolactone) (PLCL), with type I collagen or the tri-n-butylamine salt of heparin (heparin-TBA) were co-electrospun using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as a solvent. The co-electrospun fabrics (...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050086u
更新日期：2005-07-01 00:00:00
abstract：:Silkworm silk has excellent mechanical properties, biocompatibility, and promising applications in the biomedical sector. Silkworms spin silk at the beginning and end of each of their five instar stages, as well as spinning mature silk after the fifth instar. We evaluated the mechanical properties and structure of 10 ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01576
更新日期：2019-03-11 00:00:00
abstract：:A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based on three different core sizes (18, 35, 65 nm) and five polymer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300248u
更新日期：2012-05-14 00:00:00
abstract：:The long-term stability and success of orthopedic implants depend on the osseointegration process, which is strongly influenced by the biomaterial surface. A promising approach to enhance implant integration involves the modification of the surface of the implant by means of polymers that mimic the natural components ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3002683
更新日期：2012-05-14 00:00:00
abstract：:A polyethylene glycol (PEG) retinylamine (Ret-NH2) conjugate PEG-GFL-NH-Ret with a glycine-phenylalanine-leucine (GFL) spacer was synthesized for controlled oral delivery of Ret-NH2 to treat retinal degenerative diseases, including Stargardt disease (STGD) and age-related macular degeneration (AMD). The peptide spacer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501352s
更新日期：2014-12-08 00:00:00
abstract：:Relative to normal cells, tumor cells lack adequate capability of reactive oxygen scavenging. Thus, tumor cells can be selectively killed by increasing the concentration of reactive oxygen species in tumor tissue. In this report, we construct an integrated multifunctional polymeric nanoparticle which can selectively i...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01777
更新日期：2018-06-11 00:00:00
abstract：:We demonstrate the formation of polyethylene glycol (PEG) based hydrogels via oxime ligation and the photoinitiated thiol-ene 3D patterning of peptides within the hydrogel matrix postgelation. The gelation process and final mechanical strength of the hydrogels can be tuned using pH and the catalyst concentration. The ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401133r
更新日期：2013-10-14 00:00:00
abstract：:We examine the protein distribution within an electrospun polymer nanofiber using polyvinyl alcohol and bovine serum albumin as a model system. We hypothesize that the location of the protein within the nanofiber can be controlled by carefully selecting the pH and the applied polarity of the electric field as the pH a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2017146
更新日期：2012-05-14 00:00:00
abstract：:Vascular grafts that can support total replacement and maintenance by the body of the injured vessel would improve outcomes of major surgical reconstructions. Building scaffolds using components of the native vessel can encourage biological recognition by native cells as well as mimic mechanical characteristics of the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01165
更新日期：2017-11-13 00:00:00
abstract：:Human serum albumin (HSA) is known to exist as N (pH approximately 7), B (pH approximately 9), and F (pH approximately 3.5) isomeric forms and an equilibrium intermediate state (I) accumulate in the urea induced unfolding pathway of HSA around 4.8-5.2 M urea concentrations. These states displayed characteristic struct...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050996b
更新日期：2006-04-01 00:00:00
abstract：:A lipid bilayer was deposited on a 3 μm diameter polystyrene (PS) bead via hydrophobic anchoring of bicelles containing oxyamine-bearing cholesteric moieties reacting with the aldehyde functionalized bead surface. Discoidal bicelles were formed by mixing dimyristoylphosphatidylcholine (DMPC), dihexanoylphosphatidylcho...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00042
更新日期：2015-03-09 00:00:00
abstract：:Atomic force microscopy (AFM) was used to assess a new chimeric protein consisting of a fusion protein of the consensus repeat for Nephila clavipes spider dragline protein and bone sialoprotein (6mer+BSP). The elastic modulus of this protein in film form was assessed through force curves, and film surface roughness wa...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2000605
更新日期：2011-05-09 00:00:00
abstract：:The development and use of synthetic, cross-linked, macromolecular substrates with tunable elasticity has been instrumental in revealing the mechanisms by which cells sense and respond to their mechanical microenvironment. We here describe a hydrogel based on radical-free, cross-linked poly(ethylene glycol) to study t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4014827
更新日期：2014-01-13 00:00:00
abstract：:A drying environment is always a proposition faced by dynamic living organisms using water, which are driven by biopolymer-based micro- and macrostructures. Here, we introduce a drying process for aqueous liquid crystalline (LC) solutions composed of biopolymer with extremely high molecular weight components such as p...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00302
更新日期：2016-06-13 00:00:00
abstract：:Anionic copolymer systems containing sulfated monomers have great potential for delivery of cationic therapeutics, but N-isopropylacrylamide (NIPAm) 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) copolymer nanoparticles have seen limited characterization to date with regard to physical properties relevant to load...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300173x
更新日期：2012-04-09 00:00:00
abstract：:Poly(dimethyl siloxane) (PDMS) is extensively used for biomedical applications due to its low cost, ease of fabrication, high durability and flexibility, oxygen permeability, and self-healing properties. PDMS, however, has some significant drawbacks. PDMS endures unacceptably high levels of nonspecific protein fouling...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300399s
更新日期：2012-05-14 00:00:00
abstract：:The recombinant amylosucrase from Neisseria polysaccharea was used to glucosylate glycogen particles in vitro in the presence of sucrose as the glucosyl donor. The morphology and structure of the resulting insoluble products were shown to strongly depend on the initial sucrose/glycogen weight ratio. For the lower rati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050988v
更新日期：2006-06-01 00:00:00
abstract：:Injectable in situ crosslinkable biomaterials seeded with multipotent progenitor cells and coupled with minimally invasive arthroscopic techniques are an attractive alternative for treating irregularly shaped osteochondral defects. An in situ crosslinkable poly(lactide-co-ethylene oxide-co-fumarate) (PLEOF) macromer h...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060671a
更新日期：2007-03-01 00:00:00
abstract：:Despite great potential, the delivery of genetic materials into cells or tissues of interest remains challenging owing to their susceptibility to nuclease degradation, lack of permeability to the cell membrane, and short in vivo half-life, which severely restrict their widespread use in therapeutics. To surmount these...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00819
更新日期：2018-08-13 00:00:00
abstract：:A lamellar compound resulted from reaction of acrylic acid inside crystalline beta-chitin and the structure was investigated. Beta-chitin acts like a layered crystal, having stacked molecular sheets composed of parallel chains bound in one direction by intermolecular amide hydrogen bonding. Small guest molecules can b...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060643s
更新日期：2007-04-01 00:00:00
abstract：:Chitosan-dendrimer hybrids having various functional groups such as carboxyl, ester, and poly(ethylene glycol) groups were prepared successfully using dendrimer acetal by reductive N-alkylation. The synthetic procedure could be accomplished by one-step reaction without organic solvent. The degree of substitution of de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030021w
更新日期：2003-09-01 00:00:00
abstract：:We synthesized positively charged biodegradable hydrogels by cross-linking of agmatine-modified poly(ethylene glycol)-tethered fumarate (Agm-PEGF) and poly(propylene fumarate-co-ethylene glycol) (P(PF-co-EG)) to investigate the effect of the guanidino groups of the agmatine on hydrogel swelling behavior and smooth mus...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0200433
更新日期：2002-09-01 00:00:00
abstract：:Bovine serum albumin (BSA) and poly(diallyldimethylammonium chloride) (PDADMAC) spontaneously form, over a range of ionic strength I and pH, dense fluids rich in both macroions. To study their nanostructure, these coacervates were prepared at low I and high pH (strong interaction) or at high I and lower pH (weaker int...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049174p
更新日期：2005-05-01 00:00:00
abstract：:Bioprinting has become an important tool for fabricating regenerative implants and in vitro cell culture platforms. However, until today, extrusion-based bioprinting processes are limited to resolutions of hundreds of micrometers, which hamper the reproduction of intrinsic functions and morphologies of living tissues....
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01577
更新日期：2021-01-07 00:00:00
