Abstract：
:Receptor-mediated, cell-specific delivery of siRNA enables silencing of target genes in specific tissues, opening the door to powerful therapeutic options for a multitude of diseases. However, the development of delivery systems capable of targeted and effective siRNA delivery typically requires multiple steps and the use of sophisticated, orthogonal chemistries. Previously, we developed diblock copolymers consisting of dimethaminoethyl methacrylate-b-dimethylaminoethyl methacrylate-co-butyl methacrylate-co-propylacrylic acid as potent siRNA delivery systems that protect siRNA from enzymatic degradation and enable its cytosolic delivery through pH-responsive, endosomolytic behavior. (1, 2) These architectures were polymerized using a living radical polymerization method, specifically reversible addition-fragmentation chain transfer (RAFT) polymerization, which employs a chain transfer agent (CTA) to modulate the rate of reaction, resulting in polymers with low polydispersity and telechelic chain ends reflecting the chemistry of the CTA. Here we describe the straightforward, facile synthesis of a folate receptor-targeted diblock copolymer siRNA delivery system because the folate receptor is an attractive target for tumor-selective therapies as a result of its overexpression in a number of cancers. Specifically, we detail the de novo synthesis of a folate-functionalized CTA, use the folate-CTA for controlled polymerizations of diblock copolymers, and demonstrate efficient, specific cellular folate receptor interaction and in vitro gene knockdown using the folate-functionalized polymer.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Benoit DS,Srinivasan S,Shubin AD,Stayton PSdoi
10.1021/bm200485bsubject
Has Abstractpub_date
2011-07-11 00:00:00pages
2708-14issue
7eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract：:In the present study, we monitored the conformation and chemical stability of a hydrophilic bottlebrush (BB) polymer in pure water and buffered saline solutions. We correlated these parameters to lubricating and wear protecting properties. Using the surface forces apparatus (SFA), we show that the BB polymer partially...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01063
更新日期：2017-12-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8000765
更新日期：2008-07-01 00:00:00
abstract：:This study presents an efficient and systematic approach to synthesize bioapplicable porous hollow polymeric capsules (HPCs). The hydroxyl-functionalized nanoporous polymers with hollow capsular shapes could be generated via the moderate Friedel-Crafts reaction without using any hard or soft template. The numerous pri...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01520
更新日期：2020-12-17 00:00:00
abstract：:Low molecular weight random copolymers bearing protonated primary amine groups and hydrophobic alkyl groups in the side chains were synthesized and their activities against E. coli , S. aureus , human red blood cells, and human epithelial carcinoma cells (HEp-2) were quantified. The mole fraction of alkyl side chains ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900784x
更新日期：2009-11-09 00:00:00
abstract：:We studied methoxy poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PCL-b-PDMAEMA) nanoparticles as the codelivery vector of hydrophobic drug and pDNA by employing dynamic light scattering (DLS), ζ potential, transmission electron microscopy (TEM), gel retardation assay, a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100410m
更新日期：2010-09-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7007235
更新日期：2007-12-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00175
更新日期：2019-04-08 00:00:00
abstract：:Amphiphilic random, gradient, and block copolymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and n-butyl methacrylate (BMA) were synthesized by atom transfer radical polymerization (ATRP) in water/2-propanol mixtures using a methoxy-poly(ethylene glycol) (MPEG) (M(n) = 2000) macroinitiator. Kinetic studies indic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034126a
更新日期：2003-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00242
更新日期：2018-06-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800580c
更新日期：2008-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01915
更新日期：2017-03-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01525
更新日期：2021-01-06 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm061119o
更新日期：2007-06-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1001476
更新日期：2010-10-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020076i
更新日期：2003-05-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01416
更新日期：2016-02-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01831
更新日期：2017-03-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2015174
更新日期：2012-02-13 00:00:00
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journal_title：Biomacromolecules
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更新日期：2015-04-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Biomacromolecules
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更新日期：2007-01-01 00:00:00
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journal_title：Biomacromolecules
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更新日期：2002-01-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2019-04-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050653d
更新日期：2005-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1006344
更新日期：2010-10-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100209b
更新日期：2010-06-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070233j
更新日期：2007-07-01 00:00:00
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更新日期：2011-09-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100138m
更新日期：2010-06-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01577
更新日期：2021-01-07 00:00:00
