Abstract:
:New aspects concerning the mechanism of formation of chitosan physical hydrogels without any cross-linking agent were studied. The gelation took place during the evaporation of a hydroalcoholic solution of chitosan. We first demonstrated that it was possible to form a physical hydrogel from a hydrochloride form of chitosan. Chromatographic methods showed that during the gel formation, when the initial concentration is over C, the critical concentration of chain entanglement, the water and acid used for the solubilization of the polymer were both eliminated. This particular situation contributed to decrease the dielectric constant of the medium and the apparent charge density of chitosan chains, thus inducing the formation of a three-dimensional network through hydrophobic interactions and hydrogen bonding. In the gelation process, this step was kinetically determining. The speed of evaporation of water and acid were determined and different initial conditions were compared. Thus, we investigated the influence of: the initial polymer concentration, the nature of the counterion and the alcohol, the temperature and the geometry of the reactor. Our results allowed us to confirm the existence of a second critical initial concentration C, from which the evaporation of water became more difficult. We suggested that C corresponded to a reorganization of the solution involving the presence of gel precursors. Then, a mechanism of formation of physical hydrogels of chitosan in a hydroalcoholic medium could be proposed. For the first time, we demonstrated that it was possible to generate physical hydrogels in the presence of various diols, which size of the carbonated chain appeared as a limiting factor for the gelation process. These physical hydrogels of chitosan are currently used in our laboratory for tissue engineering in the treatment of third degree burns with the possibility to adapt their mechanical properties from the choice of both the acid or the alcohol used.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Boucard N,Viton C,Domard Adoi
10.1021/bm050653dkeywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
3227-37issue
6eissn
1525-7797issn
1526-4602journal_volume
6pub_type
杂志文章abstract::Although micelles are commonly used for drug delivery purposes, their long-term fate is often unknown due to photobleaching of the fluorescent labels or the use of toxic materials. Here, we present a metal-free, nontoxic, nonbleaching, fluorescent micelle that can address these shortcomings. A simple, yet versatile, p...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01653
更新日期:2016-12-12 00:00:00
abstract::Reduction-sensitive shell-sheddable glyco-nanoparticles were designed and developed based on poly(ε-caprolactone)-graft-SS-lactobionic acid (PCL-g-SS-LBA) copolymer for efficient hepatoma-targeting delivery of doxorubicin (DOX). PCL-g-SS-LBA was prepared by ring-opening copolymerization of ε-caprolactone and pyridyl d...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401749t
更新日期:2014-03-10 00:00:00
abstract::Vaginal films featuring the pH-dependent release of tenofovir (TFV) were developed for the prevention of sexual transmission of human immunodeficiency syndrome (HIV). Films based on hydroxypropyl methylcellulose and zein were prepared incorporating different plasticizers [oleic acid, lactic acid, glycerol, and polyeth...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01609
更新日期:2021-01-06 00:00:00
abstract::We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)-poly(l-lysine) particles (NPEG-PLLs). The obtained particles exhibit no toxicity while maintaining the capability to deliver a small interfering RNA sequence (siRNA) targeting the anti-apoptotic factor, survivin, in prostate c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00562
更新日期:2015-07-13 00:00:00
abstract::PEO-PPO-PEO triblock copolymers have opposing effects on lipid membrane integrity: they can behave either as membrane sealants or as membrane permeabilizers. To gain insights into their biomembrane activities, the fundamental interactions between a series of PEO-based polymers and phospholipid vesicles were investigat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300847x
更新日期:2012-09-10 00:00:00
abstract::Strong signaling cascades derived from upregulation and overexpression of growth factors such as the EGF-family (epidermal growth factors) have been crucially related to cancer pathogenesis. Gene silencing techniques to modulate the expression of oncogenes and tumor suppresor genes are a strategy that shows great prom...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01066
更新日期:2018-10-08 00:00:00
abstract::Many biological processes are regulated by reversible binding events, with these interactions between macromolecules representing the core of dynamic chemistry. As such, any attempt to gain a better understanding of such interactions, which would pave the way to the extrapolation of natural designs to create new advan...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01103
更新日期:2015-10-12 00:00:00
abstract::Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801430x
更新日期:2009-05-11 00:00:00
abstract::Degradable polymers with specifically designed functionality have wide applications in biomedical fields. We reported herein the synthesis and characterization of a water-soluble and fast-degradable polycarbonate, functionalized with tertiary amine groups in the backbone. A novel cyclic carbonate monomer, namely, 6,14...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1001476
更新日期:2010-10-11 00:00:00
abstract::This work reports on the effect of CO2 at subcritical conditions and the gaseous state on the telechelic poly(epsilon-caprolactone) polymers. The tested polymers are semi-crystalline in nature and thus the effect of functional groups and their overall contribution to foaming and formation of microstructures with open-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050223n
更新日期:2005-09-01 00:00:00
abstract::Previously, we have shown that the amphiphilic decyl esters of both D- and L-tyrosine (DELT) self-assemble in aqueous solution above their critical micelle concentration values to form long rodlike structures that can be enzymatically polymerized. In the current study, we have examined the self-assembled structures of...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049793y
更新日期:2004-09-01 00:00:00
abstract::Sulfated glycosaminoglycans (GAGs) can direct cellular processes by interacting with proteins of the extracellular matrix (ECM). In this study we characterize the interaction profiles of chemically sulfated hyaluronan (HA) and chondroitin sulfate (CS) derivatives with bone morphogenetic protein-2 (BMP-2) and investiga...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5006855
更新日期:2014-08-11 00:00:00
abstract::Molecular dynamics (MD) simulations have been applied to study the interactions between hydrophobic and hydrophilic faces of ordered cellulose chains and a single layer of graphene in explicit aqueous solvent. The hydrophobic cellulose face is predicted to form a stable complex with graphene. This interface remains so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00307
更新日期:2015-06-08 00:00:00
abstract::Lignin is a natural, renewable biopolymer synthesized by plants. It is a macromolecule consisting of aromatic structures with high density of functional groups making it an ideal precursor for the design of sustainable biomaterials for applications such as drug delivery. The rational design of these materials requires...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00822
更新日期:2017-08-14 00:00:00
abstract::The remarkable diversity of the self-assembly behavior of PEG-peptides is reviewed, including self-assemblies formed by PEG-peptides with β-sheet and α-helical (coiled-coil) peptide sequences. The modes of self-assembly in solution and in the solid state are discussed. Additionally, applications in bionanotechnology a...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm500246w
更新日期:2014-05-12 00:00:00
abstract::Five poly(betaine) brushes were prepared, and their resistance to blood plasma fouling was studied. Two carboxybetaines monomers were copolymerized with 2-hydroxyethyl methacrylate (HEMA) to prepare novel hydrogels. By increasing the content of the zwitterionic comonomer, a 4-fold increase in the water content could b...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301441x
更新日期:2012-12-10 00:00:00
abstract::The importance of the stabilization of the quaternary structure of multimeric enzymes has been illustrated using a model reaction with great industrial relevance: the enzymatic synthesis of ampicillin from 6-amino penicillanic acid (6APA) and phenylglycine methyl ester (PGM) catalyzed by the tetrameric enzyme alpha-am...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm000072i
更新日期:2001-04-01 00:00:00
abstract::The adhesion of wet regenerated cellulose to colloidal complexes formed between carboxymethyl cellulose (CMC) and polyvinylamine (PVAm) was evaluated by measuring the force to delaminate pairs of regenerated cellulose membranes bound together with polyelectrolyte complex. The most important parameter was the surface c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070307r
更新日期:2007-07-01 00:00:00
abstract::Adhesive hydrogels were prepared by copolymerizing dopamine methacrylamide with either acrylic acid (AAc) or N-(3-aminopropyl)methacrylamide hydrochloride (APMH). The effect of incorporating the anionic and cationic side chains on the oxidation state of catechol was characterized using the FOX assay to track the produ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01311
更新日期:2018-05-14 00:00:00
abstract::Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was applied to investigate the interaction of bovine serum albumin (BSA) and fibrinogen with a biomedical-grade 316LVM stainless steel surface, in terms of the adsorption thermodynamics and adsorption-induced secondary structure changes of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070289d
更新日期:2007-09-01 00:00:00
abstract::Positive strand RNA viruses replicate in specialized niches called membranous web within the cytoplasm of host cells. These virus replication organelles sequester viral proteins, RNA, and a variety of host factors within a fluid, amorphous matrix of clusters of endoplasmic reticulum (ER) derived vesicles. They are tho...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00081
更新日期:2019-04-08 00:00:00
abstract::Polycations often suffer from the irreconcilable inconsistency between transfection efficiency and toxicity. Polymers with high molecular weight (MW) and cationic charge feature potent gene delivery capabilities, while in the meantime suffer from strong chemotoxicity, restricted intracellular DNA release, and low stab...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00180
更新日期:2015-04-13 00:00:00
abstract::Developing new protein-based materials with a programmable assembly is of great scientific interest and technological importance. Inspired by nature's use of post-translational modifications (PTMs) to control the function and location of proteins, we have leveraged lipidation-the PTM of proteins with lipids-to synthes...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01380
更新日期:2020-02-10 00:00:00
abstract::Supramolecular nanogel, a physically cross-linked nanosize hydrogel, spontaneously self-assembles in aqueous solution via secondary interactions and is thus of great interest in nanomedicine as a drug carrier. We developed a versatile method for supramolecular nanogel self-assembled by electrostatic interaction betwee...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400846h
更新日期:2013-10-14 00:00:00
abstract::Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049941k
更新日期:2004-07-01 00:00:00
abstract::We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on particles were obtained by the formation of multicomposition...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400680d
更新日期:2013-08-12 00:00:00
abstract::The barrier functions of the stratum corneum and the epidermal layers present a tremendous challenge in achieving effective transdermal delivery of drug molecules. Although a few reports have shown that poly(amidoamine) (PAMAM) dendrimers are effective skin-penetration enhancers, little is known regarding the fundamen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300545b
更新日期:2012-07-09 00:00:00
abstract::Transparent and bendable regenerated cellulose films prepared from aqueous alkali (NaOH or LiOH)/urea (AU) solutions exhibit high oxygen barrier properties, which are superior to those of conventional cellophane, poly(vinylidene chloride), and poly(vinyl alcohol). Series of AU cellulose films are prepared from differe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200766v
更新日期:2011-07-11 00:00:00
abstract::Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bioimaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on the Pepino mosaic virus (PepMV), a relative of the establishe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01365
更新日期:2019-01-14 00:00:00
abstract::This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800234r
更新日期:2008-09-01 00:00:00