Abstract:
:Sulfated glycosaminoglycans (GAGs) can direct cellular processes by interacting with proteins of the extracellular matrix (ECM). In this study we characterize the interaction profiles of chemically sulfated hyaluronan (HA) and chondroitin sulfate (CS) derivatives with bone morphogenetic protein-2 (BMP-2) and investigate their relevance for complex formation with the receptor BMPR-IA. These goals were addressed by surface plasmon resonance (SPR) and ELISA in combination with molecular modeling and dynamics simulation. We found not only the interaction of BMP-2 with GAGs to be dependent on the type and sulfation of GAGs but also BMP-2/GAG/BMPR-IA complex formation. The conformational plasticity of the BMP-2 N-termini plays a key role in the structural and thermodynamic characteristics of the BMP-2/GAG/BMPR-IA system. Hence we propose a model that provides direct insights into the importance of the structural and dynamical properties of the BMP-2/BMPR-IA system for its regulation by sulfated GAGs, in which structural asymmetry plays a key role.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Hintze V,Samsonov SA,Anselmi M,Moeller S,Becher J,Schnabelrauch M,Scharnweber D,Pisabarro MTdoi
10.1021/bm5006855subject
Has Abstractpub_date
2014-08-11 00:00:00pages
3083-92issue
8eissn
1525-7797issn
1526-4602journal_volume
15pub_type
杂志文章abstract::Thermoresponsive anionic copolymer brushes, poly(N-isopropylacrylamide-co-acrylic acid-co-tert-butylacrylamide) [P(IPAAm-co-AAc-co-tBAAm)], were grafted onto a monolithic silica rod column through surface-initiated atom-transfer radical polymerization (ATRP) to prepare an effective thermoresponsive anionic chromatogra...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401779r
更新日期:2014-04-14 00:00:00
abstract::Highly cross-linked networks formed by the photoinitiated polymerization of multifunctional monomers are finding application in the field of biomaterials because of their chemical versatility, reaction control, and ability to polymerize under physiological conditions. Typically, degradation is introduced into these ne...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025677o
更新日期:2003-01-01 00:00:00
abstract::Amphiphilic block copolymers containing polypeptides can self-assemble into a variety of nonspherical structures arising from strong interactions between peptide units. Here, we report the synthesis of a pH-responsive poly(ethyl glycol)-block-poly(l-glutamic acid)-block-poly(N-octylglycine) (PEG-b-PGA-b-PNOG) triblock...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01014
更新日期:2017-10-09 00:00:00
abstract::A temperature-responsive hydrogel composed of aqueous methylcellulose (MC) blended with distinct concentrations of PBS was prepared and characterized. The developed MC hydrogel underwent a sol-gel reversible transition upon heating or cooling at approximately 32 degrees C. This temperature-responsive hydrogel was empl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0704166
更新日期:2007-09-01 00:00:00
abstract::Neutron reflectometry was used to investigate effects of calcium ions on the interfacial behavior of beta-casein at the silicon oxide-aqueous solution interface. The structural characteristics of the adsorbed layer were determined from reflectivity curves fitted to three- and two-layer optical models. The results show...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034301n
更新日期:2004-03-01 00:00:00
abstract::We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700690p
更新日期:2007-11-01 00:00:00
abstract::Recent developments in ionic liquid electrolytes for cellulose or biomass dissolution has also allowed for high-resolution 1H and 13C NMR on very high molecular weight cellulose. This permits the development of advanced liquid-state quantitative NMR methods for characterization of unsubstituted and low degree of subst...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00295
更新日期:2018-07-09 00:00:00
abstract::The polymerization of N-isopropylacrylamide (NIPAAm) with ionizable monomers results in pH-responsive lower critical solution temperature (LCST) polymer which works in an ionization-dependent manner. However, gradual ionization of the comonomer occurs at a broad pH range due to the electrostatic field generated by the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01465
更新日期:2019-04-08 00:00:00
abstract::Nearly monodispersed silica nanoparticles having a controlled size from 5 to 450 nm were synthesized via a sol-gel process, and then the optimum conditions for the surface treatment of the synthesized silica nanoparticles with a silane coupling agent (i.e., 3-methacryloxypropyltrimethoxysilane (gamma-MPS)) were explor...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060560b
更新日期:2007-01-01 00:00:00
abstract::New applications in regenerative biotechnology require the ability to understand and control protein-surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein ads...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050446d
更新日期:2005-11-01 00:00:00
abstract::The development of sustainable materials by employing natural and nontoxic resources has been attracting much attention over the previous years. In this work, we discuss for the first time the chemical combination between resorcinol diglycidyl ether (RDGE), an aromatic biobased thermosetting monomer, and polyhydroxybu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00876
更新日期:2020-08-10 00:00:00
abstract::Nature shows excellent control over the mechanics of fibrous hydrogels by assembling protein fibers into bundles of well-defined dimensions. Yet, obtaining artificial materials displaying controlled bundling remains a challenge. Here, we developed genetically engineered protein-based polymers functionalized with hepar...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00276
更新日期:2016-06-13 00:00:00
abstract::Chitin is a nitrogen-rich polysaccharide that is abundant in crustaceans, mollusks, insects, and fungi and is the second most abundant organic material found in nature next to cellulose. Chitosan, the N-deacetylated derivative of chitin, is environmentally friendly, nontoxic, biodegradable, and antibacterial. Fibrous ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7006983
更新日期:2007-09-01 00:00:00
abstract::We report the rational design of a chitosan-based drug delivery system. The chitosan derivative N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride (HTCC) was ionically cross-linked by sodium tripolyphosphate (TPP) to form sub-200-nm microgels that are responsive to pH changes. When these microgels were loaded ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050912z
更新日期:2006-05-01 00:00:00
abstract::The positive interaction of materials with tissues is an important step in regenerative medicine strategies. Hydrogels that are obtained from polysaccharides and proteins are expected to mimic the natural cartilage environment and thus provide an optimum milleu for tissue growth and regeneration. In this work, novel h...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800874q
更新日期:2008-10-01 00:00:00
abstract::Mammalian synovial joints are extremely efficient lubrication systems reaching friction coefficient μ as low as 0.001 at high pressures (up to 100 atm) and shear rates (up to 10(6) to 10(7) Hz); however, despite much previous work, the exact mechanism responsible for this behavior is still unknown. In this work, we st...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2004912
更新日期:2011-10-10 00:00:00
abstract::Homocystinuria due to loss of cystathionine beta-synthase (CBS) causes accumulation of homocysteine and depletion of cysteine. Current treatments are suboptimal, and thus the development of an enzyme replacement therapy based on PEGylated human truncated CBS (PEG-CBS) has been initiated. Attenuation of potency was obs...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00154
更新日期:2017-06-12 00:00:00
abstract::Reversible addition-fragmentation chain transfer (RAFT) radical polymerization, mediated by 4-cyanopentanoic acid dithiobenzoate and 4,4'-azobis(4-cyanovaleric acid) (V-501) in water at 70 degrees C, of biocompatible 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) yields a macro-chain transfer agent (CTA) that was em...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8014945
更新日期:2009-04-13 00:00:00
abstract::Bacterial cellulose (BC) is a polysaccharide known as a suitable matrix for proper wound healing. To improve this ability, BC was functionalized with silk sericin (SS) that has cytoprotective and mitogenic effects. The composites obtained by solution impregnation were stabilized by hydrogen bonds, and SS could be rele...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00995
更新日期:2016-09-12 00:00:00
abstract::Properties of Aloe vera galacturonate hydrogels formed via Ca(2+) crosslinking have been studied in regard to key parameters influencing gel formation including molecular weight, ionic strength, and molar ratio of Ca(2+) to COO(-) functionality. Dynamic oscillatory rheology and pulsed field gradient NMR (PFG-NMR) stud...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8008457
更新日期:2008-11-01 00:00:00
abstract::There is compelling evidence that, beyond their traditional role in hemostasis and thrombosis, platelets play a significant role in mediating hematologic mechanisms of tumor metastasis by directly and indirectly interacting with pro-metastatic cancer cells. With this rationale, we hypothesized that platelets can be an...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301996p
更新日期:2013-03-11 00:00:00
abstract::n-Heptyl α-d-mannose (HM) is a nanomolar antagonist of FimH, a virulence factor of E. coli. Herein we report on the construction of multivalent HM-based glycopolymers as potent antiadhesives of type 1 piliated E. coli. We investigate glycopolymer/FimH and glycopolymer/bacteria interactions and show that HM-based glyco...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00413
更新日期:2015-06-08 00:00:00
abstract::Biodegradable multilayered capsules encapsulating basic fibroblast growth factor (bFGF) were developed as a cytokine release carrier for drug delivery systems. The multilayered hollow capsules were fabricated via the layer-by-layer (LbL) assembly of chitosan (CT) and dextran sulfate (Dex). The bFGF was encapsulated in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800321w
更新日期:2008-08-01 00:00:00
abstract::Single-molecule measurements of the interaction of leukocyte function-associated antigen-1 (LFA-1), expressed on Jurkat T cells, with intercellular adhesion molecules-1 and -2 (ICAM-1 and ICAM-2) were conducted using atomic force microscopy (AFM). The force spectra (i.e., unbinding force versus loading rate) of both t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060559c
更新日期:2006-11-01 00:00:00
abstract::Polycations often suffer from the irreconcilable inconsistency between transfection efficiency and toxicity. Polymers with high molecular weight (MW) and cationic charge feature potent gene delivery capabilities, while in the meantime suffer from strong chemotoxicity, restricted intracellular DNA release, and low stab...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00180
更新日期:2015-04-13 00:00:00
abstract::Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801430x
更新日期:2009-05-11 00:00:00
abstract::In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functiona...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501613u
更新日期:2015-03-09 00:00:00
abstract::A dual-targeted siRNA nanocarrier has been synthesized and validated that is selectively activated in environments where there is colocalization of two breast cancer hallmarks, elevated matrix metalloproteinase (MMP) activity and folate receptor overexpression. This siRNA nanocarrier is self-assembled from two polymer...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501394m
更新日期:2015-01-12 00:00:00
abstract::Tannic acid (TA) can form stable complexes with proteins, attracting significant attention as protein delivery systems. However, its systemic application has been limited due to nonspecific interaction. Here, we report a simple technique to prepare systemically applicable protein delivery systems using sequential self...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00903
更新日期:2020-09-14 00:00:00
abstract::Polymer therapeutics, including polymeric drugs and polymer-protein conjugates, are clinically established as first-generation nanomedicines. Knowing that the coiled-coil peptide motif is fundamentally important in the regulation of many cellular and pathological processes, the aim of these studies was to examine the ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100843e
更新日期:2011-01-10 00:00:00