Abstract:
:There is compelling evidence that, beyond their traditional role in hemostasis and thrombosis, platelets play a significant role in mediating hematologic mechanisms of tumor metastasis by directly and indirectly interacting with pro-metastatic cancer cells. With this rationale, we hypothesized that platelets can be an effective paradigm to develop nanomedicine platforms that utilize platelet-mimetic interaction mechanisms for targeted diagnosis and therapy of metastatic cancer cells. Here we report on our investigation of the development of nanoconstructs that interact with metastatic cancer cells via platelet-mimetic heteromultivalent ligand-receptor pathways. For our studies, pro-metastatic human breast cancer cell line MDA-MB-231 was studied for its surface expression of platelet-interactive receptors, in comparison to another low-metastatic human breast cancer cell line, MCF-7. Certain platelet-interactive receptors were found to be significantly overexpressed on the MDA-MB-231 cells, and these cells showed significantly enhanced binding interactions with active platelets compared to MCF-7 cells. Based upon these observations, two specific receptor interactions were selected, and corresponding ligands were engineered onto the surface of liposomes as model nanoconstructs, to enable platelet-mimetic binding to the cancer cells. Our model platelet-mimetic liposomal constructs showed enhanced targeting and attachment of MDA-MB-231 cells compared to the MCF-7 cells. These results demonstrate the promise of utilizing platelet-mimetic constructs in modifying nanovehicle constructs for metastasis-targeted drug as well as modifying surfaces for ex-vivo cell enrichment diagnostic technologies.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Modery-Pawlowski CL,Master AM,Pan V,Howard GP,Sen Gupta Adoi
10.1021/bm301996psubject
Has Abstractpub_date
2013-03-11 00:00:00pages
910-9issue
3eissn
1525-7797issn
1526-4602journal_volume
14pub_type
杂志文章abstract::Glycosidases have long been used for the synthesis of glycosides by transglycosylation reactions. Especially glycosidases from hyperthermophilic bacteria are useful for reactions under extreme reaction conditions, e.g., in the presence of organic solvents. We herein report the facile enzymatic synthesis and purificati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01647
更新日期:2020-02-10 00:00:00
abstract::The formation of a microvasculature is regulated in large part by cell-cell interactions. Ephrins and their Eph receptors mediate cell adhesion, repulsion, and migration, all critical processes in angiogenesis. (1) Here we use a covalently immobilized ephrinA1, conjugated to poly(ethylene glycol), to induce vessel for...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200492h
更新日期:2011-07-11 00:00:00
abstract::New applications in regenerative biotechnology require the ability to understand and control protein-surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein ads...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050446d
更新日期:2005-11-01 00:00:00
abstract::Noncontact mode atomic force microscopy was used to investigate native silk proteins prepared in different ways. Low protein concentrations revealed that single protein molecules exhibit a simple, round shape with apparent diameters of 20-25 nm. Shearing the native protein solutions after extraction from the gland and...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201509b
更新日期:2012-03-12 00:00:00
abstract::Nanofibrous biocomposite scaffolds of type I collagen and nanohydroxyapatite (nanoHA) of varying compositions (wt %) were prepared by electrostatic cospinning. The scaffolds were characterized for structure and morphology by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060879w
更新日期:2007-02-01 00:00:00
abstract::Atomic force microscopy (AFM) was used to assess a new chimeric protein consisting of a fusion protein of the consensus repeat for Nephila clavipes spider dragline protein and bone sialoprotein (6mer+BSP). The elastic modulus of this protein in film form was assessed through force curves, and film surface roughness wa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2000605
更新日期:2011-05-09 00:00:00
abstract::The viscous mucus coating that adheres to the epithelial surfaces of mammalian organs provides protection for the underlying tissues and is an efficient barrier to drug delivery. Pulsed-gradient spin-echo NMR and small-angle neutron scattering have been used to study the aqueous solution interaction of various model p...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9009667
更新日期:2010-01-11 00:00:00
abstract::Despite the wide interest raised by lung administration of nanoparticles (NPs) for the treatment of various diseases, little information is available on their effect toward the airway epithelial barrier function. In this study, the potential damage of the pulmonary epithelium upon exposure to poly(lactide-co-glycolide...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201226x
更新日期:2011-11-14 00:00:00
abstract::Many dental restorative dental composites still utilize 2,2-bis[4-(2-hydroxy-3-methacryloyloxy propoxy) phenyl] propane (Bis-GMA) as base resin. The high viscosity of Bis-GMA necessitates dilution with dimethacrylate ethers of low viscosity such as triethylene glycol dimethacrylate (TEGDMA). However, increased amounts...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050491l
更新日期:2006-01-01 00:00:00
abstract::Detailed structural characterization of protein-polymer conjugates and understanding of the interactions between covalently attached polymers and biomolecules will build a foundation to design and synthesize hybrid biomaterials. Conjugates based on simple protein structures are ideal model system to achieve these ends...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300561y
更新日期:2012-06-11 00:00:00
abstract::The molecular recognition features of a DNA-sensitive fluorescent bioconjugate capable of targeting a specific DNA sequence with high efficiency are described. The bioconjugate combines a polypeptide from the Tc3 transposase DNA-binding domain with the dsDNA-sensitive fluorophore thiazole orange. Fluorescence spectros...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070334h
更新日期:2007-11-01 00:00:00
abstract::The extracellular matrix is an environment rich with structural, mechanical, and molecular signals that can impact cell biology. Traditional approaches in hydrogel biomaterial design often rely on modifying the concentration of cross-linking groups to adjust mechanical properties. However, this strategy provides limit...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00118
更新日期:2017-04-10 00:00:00
abstract::We report an enzyme-based method for the in situ entrapment of cells within a biopolymeric hydrogel matrix. Specifically, we used a calcium-independent microbial transglutaminase that is known to cross-link proteins and observed that it catalyzes the formation of gels from a pre-gel solution containing 10% gelatin and...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034145k
更新日期:2003-11-01 00:00:00
abstract::A unified kinetic pathway for the enzyme-catalyzed polymerization and degradation of poly(ε-caprolactone) was developed. This model tracks the complete distribution of individual chain lengths, both enzyme-bound and in solution, and successfully predicts monomer conversion and the molecular mass distribution as a func...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009312
更新日期:2011-09-12 00:00:00
abstract::The aim of this investigation was to understand and engineer the interactions between endothelial cells and the electrospun (ES) polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) nanofiber surfaces and evaluate their potential for endothelialization. Elastomeric PVDF-HFP samples were electrospun to evaluate th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401825c
更新日期:2014-04-14 00:00:00
abstract::The encapsulation of live cells with polymeric coat-ings is a versatile approach to modulate or control the response cells to their environment. The layer-by-layer (LbL) self-assembly of nonimmunogenic polyelectrolytes is employed here to attenuate or suppress the binding of antibodies to live red blood cells (RBCs) a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101200c
更新日期:2011-03-14 00:00:00
abstract::A scanning laser system has been used to generate three-dimensional trimethylolpropane trimethacrylate (TRIM) cross-linked poly(2-hydroxylethyl methacrylate) polymer microstructures through azo-bis(isobutyro)nitrile (AIBN) photopolymerization using a 20 x 0.5 NA microscope objective and 365 nm laser excitation. Macrop...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0506952
更新日期:2006-03-01 00:00:00
abstract::Silk fibroin hydrogels with tunable properties could be obtained from aqueous fibroin solutions (4.2 w/v %) in a short period of time. This was achieved by the addition of ethylene glycol diglycidyl ether (EGDE) into the reaction solution. Introduction of EGDE cross-links between the fibroin molecules decreases the mo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300006r
更新日期:2012-04-09 00:00:00
abstract::Functionally designed elastomeric nanofiber fabrics made of the equimolar copolyester, poly(L-lactide-co-epsilon-caprolactone) (PLCL), with type I collagen or the tri-n-butylamine salt of heparin (heparin-TBA) were co-electrospun using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as a solvent. The co-electrospun fabrics (...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050086u
更新日期:2005-07-01 00:00:00
abstract::Electrospinning is a versatile process used to prepare micro- and nano- sized fibers from various polymers dissolved in volatile solvents. In this report, cellulose and cellulose-heparin composite fibers are prepared from nonvolatile room temperature ionic liquid (RTIL) solvents by electrospinning. RTILs are extracted...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050837s
更新日期:2006-02-01 00:00:00
abstract::We have designed α-helical peptides de novo that can induce aggregation of various kinds of cells by focusing on physicochemical properties such as hydrophobicity, net charges, and amphipathicity. It is shown that peptide hydrophobicity is the key factor to determine capabilities for cell aggregation while peptide net...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm4014414
更新日期:2014-02-10 00:00:00
abstract::Oriented immobilization of proteins is an important step in creating protein-based functional materials. In this study, a method was developed to orient proteins on hydroxyapatite (HA) surfaces, a widely used bone implant material, to improve protein bioactivity by employing enhanced green fluorescent protein (EGFP) a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201865r
更新日期:2012-06-11 00:00:00
abstract::Twenty-five kDa polyethylenimine (PEI) is one of the most efficient nonviral gene transfer agents currently applied as a golden standard for in vitro transfection. In this study, novel 25 kDa PEI derivatives with reductively cleavable cystamine periphery (PEI-Cys) were designed to reduce carrier-associated cytotoxicit...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101364f
更新日期:2011-04-11 00:00:00
abstract::Dynamic mechanical properties of aqueous solutions of hydroxypropyl methyl cellulose (HPMC) were investigated using oscillatory shear measurements. The structure was investigated with light scattering. A systematic investigation of the frequency dependence of the shear moduli showed that HPMC forms two distinct interp...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401554s
更新日期:2014-01-13 00:00:00
abstract::Under the right conditions, some biological systems can maintain high viability after being frozen and thawed, but many others (e.g., organs and many mammalian cells) cannot. To increase the rates of post-thaw viability and widen the library of living cells and tissues that can be stored frozen, an improved understand...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00392
更新日期:2020-08-10 00:00:00
abstract::We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe3O4 nanoparticles that were synthesized using the (Fe3+/Fe2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupli...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01778
更新日期:2017-03-13 00:00:00
abstract::The formation of a suitable extracellular matrix (ECM) that promotes cell adhesion, organization, and proliferation is essential within biomaterial scaffolds for tissue engineering applications. In this work, short elastin mimetic peptide sequences, EM-19 and EM-23, were engineered to mimic the active motifs of human ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101214f
更新日期:2011-02-14 00:00:00
abstract::The helical coiled coil is a well-studied folding motif that can be used for the design of nanometer-sized bioinspired fibrous structures with potential applications as functional materials. A two-component system of coiled coil based model peptides is investigated, which forms, under acidic conditions, uniform, hundr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300882d
更新日期:2012-11-12 00:00:00
abstract::Stability of four dissimilar basic proteins (chymotrypsinogen A, ribonuclease A, cytochrome c, lysozyme) in the complex with four polyanions (heparin, poly(vinylsulfate), poly(4-styrene-sulfonate), Nafion) has been studied by differential scanning calorimetry. The polyanions were chosen because of their different char...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900480t
更新日期:2009-09-14 00:00:00
abstract::The structure of an extracellular polysaccharide (EPS) from Streptococcus thermophilus THS has been determined. A combination of component analysis, methylation analysis and NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by two-dimensi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0496514
更新日期:2005-01-01 00:00:00