Abstract：
:Under the right conditions, some biological systems can maintain high viability after being frozen and thawed, but many others (e.g., organs and many mammalian cells) cannot. To increase the rates of post-thaw viability and widen the library of living cells and tissues that can be stored frozen, an improved understanding of the mode of action of polymeric cryoprotectants is required. Here, we present a polymeric cryoprotectant, poly(methyl glycidyl sulfoxide) (PMGS), that achieved higher post-thaw viability for fibroblast cells than its small-molecule analogue dimethyl sulfoxide. By limiting the amount of water that freezes and facilitating cellular dehydration after ice nucleation, PMGS mitigates the mechanical and osmotic stresses that the freezing of water imparts on cells and facilitates higher-temperature vitrification of the remaining unfrozen volume. The development of PMGS advances a fundamental physical understanding of polymer-mediated cryopreservation, which enables new material design for long-term preservation of complex cellular networks and tissue.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Burkey AA,Hillsley A,Harris DT,Baltzegar JR,Zhang DY,Sprague WW,Rosales AM,Lynd NAdoi
10.1021/acs.biomac.0c00392subject
Has Abstractpub_date
2020-08-10 00:00:00pages
3047-3055issue
8eissn
1525-7797issn
1526-4602journal_volume
21pub_type
杂志文章abstract：:Hydrophobic methacrylic monomers were polymerized in aqueous media using methylated (1.8)-beta-cyclodextrin (MeCD) additives. Hydrophobic monomers tert-butyl methacrylate (tBuMA), cyclohexyl methacrylate (CMA), and 2-ethylhexyl methacrylate (2EHMA) were each dissolved in chloroform with MeCD. Chloroform was then evapo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0055706
更新日期：2000-01-01 00:00:00
abstract：:Polycations often suffer from the irreconcilable inconsistency between transfection efficiency and toxicity. Polymers with high molecular weight (MW) and cationic charge feature potent gene delivery capabilities, while in the meantime suffer from strong chemotoxicity, restricted intracellular DNA release, and low stab...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00180
更新日期：2015-04-13 00:00:00
abstract：:The design of functional systems with sizes in the nanometer range is a key challenge in fields such as biomedicine, nanotechnology, and engineering. Some of the most promising materials nowadays consist of self-assembling peptides or peptide-polymer hybrid materials because of their versatility and the resulting prop...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.7b00764
更新日期：2017-11-13 00:00:00
abstract：:The new combinations of monomers presented in this work were evaluated in order to create an elastic material for potential application in soft tissue engineering. Thermoplastic elastomers (TPE) of trimethylene carbonate (TMC) with L-lactide (LLA) and 1,5-dioxepan-2-one (DXO) have been synthesized using a cyclic five-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060081c
更新日期：2006-05-01 00:00:00
abstract：:X-ray analytical techniques are increasingly being used to study manuscripts and works of art on paper, whether with laboratory equipment or synchrotron sources. However, it is difficult to anticipate the impact of X-ray photons on paper- and cellulose-based artifacts, particularly due to the large variety of their co...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00512
更新日期：2020-07-13 00:00:00
abstract：:In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500750v
更新日期：2014-07-14 00:00:00
abstract：:A comprehensive knowledge of the in vivo fate of polymers is essential for their potential application in humans. In this study, the body distribution, accumulation, and elimination processes of intraperitoneally (ip) administered poly(vinyl alcohol) (PVA) in mice were investigated in detail. Two derivatives of PVA (1...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200899h
更新日期：2011-10-10 00:00:00
abstract：:Macromolecular dynamics in biological systems, which play a crucial role for biomolecular function and activity at ambient temperature, depend strongly on moisture content. Yet, a generally accepted quantitative model of hydration-dependent phenomena based on local relaxation and diffusive dynamics of both polymer and...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00191
更新日期：2018-07-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01057
更新日期：2017-11-13 00:00:00
abstract：:In situ cross-linkable hybrid hydrogels composed of gelatin and 4-arm-polypropylene oxide-polyethylene oxide (Tetronic) was developed as an injectable scaffold for tissue regeneration. The gelatin was modified by hydroxyphenyl propionic acid (HPA) and the Tetronic was conjugated with tyramines (Tet-TA). The hydrogels ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201712z
更新日期：2012-03-12 00:00:00
abstract：:Sequential copolymerizations of trimethylene carbonate (TMC) and l-lactide (LLA) were performed with 2,2-dibutyl-2-stanna-1,3-oxepane as a bifunctional cyclic initiator. The block lengths were varied via the monomer/initiator and via the TMC/l-lactide ratio. The cyclic triblock copolymers were transformed in situ into...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049393a
更新日期：2005-01-01 00:00:00
abstract：:RNA interference (RNAi) requires the intracellular delivery of RNA molecules to initiate the neutralization of targeted mRNA molecules, inhibiting the expression or translation of the targeted gene. Current polymers and lipids that are used to deliver RNA molecules are generally required to be positively charged, to a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01321
更新日期：2019-01-14 00:00:00
abstract：:Amyloid-like fibrils are prepared from protein in the lab by controlled heat treatments, yet these must be further assembled to match the desirable mechanical and structural properties of biological fibers. Here, β-lactoglobulin fibrils were incorporated into poly(ethylene oxide) fibers of 40-180 nm diameter by electr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00500
更新日期：2020-07-13 00:00:00
abstract：:Semicrystalline, thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOx) layers covalently bonded to glass or silica wafers were obtained via the surface-termination of the living polymer chains. Polymer solutions in acetonitrile were exposed to 50 °C for various time periods and were poured onto the functionalized sol...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00745
更新日期：2015-09-14 00:00:00
abstract：:Ferulic acid-based polymers with aliphatic linkages have been previously synthesized via solution polymerization methods, yet they feature relatively slow ferulic acid release rates (∼11 months to 100% completion). To achieve a more rapid release rate as required in skin care formulations, ferulic acid-based polymers ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00824
更新日期：2015-09-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9003767
更新日期：2009-07-13 00:00:00
abstract：:Herein, we developed a new gene delivery vector by grafting a betaine monomer (N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate, DMAAPS) onto 25 KDa polyethylenimine (PEI 25K) via the Michael addition reaction. The graft ratio for betaine on PEI polymer could be readily controlled, and in this study three PEI-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301826m
更新日期：2013-03-11 00:00:00
abstract：:We report a simple and novel top-down method based on a drop-casting process for the controlled synthesis of all-bone-minerals biomimetic multicomponent bionanocomposites. Integration of micro- and nanoscale binary features into nanofibrous biocompatible polymer scaffold structures is successfully demonstrated. Compos...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1009359
更新日期：2010-10-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01469
更新日期：2017-03-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0494038
更新日期：2005-03-01 00:00:00
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journal_title：Biomacromolecules
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更新日期：2015-07-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00822
更新日期：2017-08-14 00:00:00
abstract：:Neutron reflectometry was used to investigate the time-dependent beta-casein adsorption at the silica-aqueous solution interface. The transient and steady-state structural characteristics of the adsorbed layer were determined from reflectivity curves, fitted to three-layer and two-layer models. The results show that t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0155221
更新日期：2001-10-01 00:00:00
abstract：:An underwater bioadhesive generally comprises a multiprotein complex that provides a molecular basis for self-assembly. We report here a new class of self-assembling peptide inspired by a 20 kDa barnacle cement protein. Studies on the chemically synthesized 24-residue peptide have revealed that (1) it underwent irreve...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0612236
更新日期：2007-06-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01775
更新日期：2020-06-08 00:00:00
abstract：:The development of sustainable materials by employing natural and nontoxic resources has been attracting much attention over the previous years. In this work, we discuss for the first time the chemical combination between resorcinol diglycidyl ether (RDGE), an aromatic biobased thermosetting monomer, and polyhydroxybu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00876
更新日期：2020-08-10 00:00:00
abstract：:Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100138m
更新日期：2010-06-14 00:00:00
abstract：:Assembly of (bio)polymers into long-range anisotropic nanostructured gels and aerogels is of great interest in advanced material engineering since it enables directional tuning of properties, such as diffusivity, light, heat, and sound propagation, cell proliferation, and mechanical properties. Here we present an appr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01278
更新日期：2018-11-12 00:00:00
abstract：:This study is devoted to developing amphiphilic block polymers based on phenylborate ester, which can self-assemble to form nanoparticles, as a glucose-sensitive drug carrier. Poly(ethylene glycol)-block-poly[(2-phenylboronic esters-1,3-dioxane-5-ethyl) methylacrylate] (MPEG5000-block-PBDEMA) was fabricated with MPEG5...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3003286
更新日期：2012-06-11 00:00:00
abstract：:Cancer immunotherapy can be augmented with toll-like receptor agonist (TLRa) adjuvants, which interact with immune cells to elicit potent immune activation. Despite their potential, use of many TLRa compounds has been limited clinically due to their extreme potency and lack of pharmacokinetic control, causing systemic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00812
更新日期：2020-09-14 00:00:00
