Abstract:
:Semicrystalline, thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOx) layers covalently bonded to glass or silica wafers were obtained via the surface-termination of the living polymer chains. Polymer solutions in acetonitrile were exposed to 50 °C for various time periods and were poured onto the functionalized solid wafers. Fibrillar crystallites formed in polymerization solutions settled down onto the wafers next to the amorphous polymer. The amount of crystallites adsorbed on thermoresponsive polymer layers depended on the annealing time of the PIPOx solution. The wettability of PIPOx layers decreased with the increasing amount of crystallites. The higher content of crystallites weakened the temperature response of the layer, as evidenced by the philicity and thickness measurements. Semicrystalline thermoresponsive PIPOx layers were used as biomaterials for human dermal fibroblasts (HDFs) culture and detachment. The presence of crystallites on the PIPOx layers promoted the proliferation of HDFs. Changes in the physicochemical properties of the layer, caused by the temperature response of the polymer, led to the change in the cells shape from a spindle-like to an ellipsoidal shape, which resulted in their detachment. A supporting membrane was used to assist the detachment of the cells from PIPOx biosurfaces and to prevent the rolling of the sheet.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Oleszko N,Wałach W,Utrata-Wesołek A,Kowalczuk A,Trzebicka B,Klama-Baryła A,Hoff-Lenczewska D,Kawecki M,Lesiak M,Sieroń AL,Dworak Adoi
10.1021/acs.biomac.5b00745subject
Has Abstractpub_date
2015-09-14 00:00:00pages
2805-13issue
9eissn
1525-7797issn
1526-4602journal_volume
16pub_type
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