Abstract：
:As a programmable biopolymer, DNA has shown great potential in the fabrication and construction of nanometer-scale assemblies and devices. In this report, we described a strategy for efficient manipulation of gold nanoparticle-bound DNA using restriction endonuclease. The digestion efficiency of this restriction enzyme was studied by varying the surface coverage of stabilizer, the size of nanoparticles, as well as the distance between the nanoparticle surface and the enzyme-cutting site of particle-bound DNA. We found that the surface coverage of stabilizer is crucial for achieving high digestion efficiency. In addition, this stabilizer surface coverage can be tailored by varying the ion strength of the system. Based on the results of polyacrylamide gel electrophoresis and fluorescent study, a high digestion efficiency of 90+% for particle-bound DNA was achieved for the first time. This restriction enzyme manipulation can be considered as an additional level of control of the particle-bound DNA and is expected to be applied to manipulate more complicated nanostructures assembled by DNA.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Qin WJ,Yung LYdoi
10.1021/bm060517osubject
Has Abstractpub_date
2006-11-01 00:00:00pages
3047-51issue
11eissn
1525-7797issn
1526-4602journal_volume
7pub_type
杂志文章abstract：:Deproteinized natural rubber latex (DPNR-latex) was treated with lipase and phosphatase in order to analyze the structure of the chain-end group (alpha-terminal). The enzymatic treatment decreased the content of long-chain fatty acid ester groups in DPNR from about 6 to 2 mol per rubber molecule. The molecular weight ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm058003x
更新日期：2005-07-01 00:00:00
abstract：:Here we present time-resolved in situ transmission electron microscopy (TEM) observations and real-time manipulation of nematic ordered cellulose and ultradrawn polyethylene films. Drawn films of these two polymers exhibited a unique response to the low-dose electron beam. Electron beam damage was minimal based on ret...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060546z
更新日期：2007-01-01 00:00:00
abstract：:Genome-editing technologies hold tremendous potential for treating genetic diseases. However, the efficient and safe delivery of genome-editing elements to the location of interest, and the achievement of specific targeted gene correction without off-target side effect remains a big challenge. In this Perspective, we ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00783
更新日期：2019-09-09 00:00:00
abstract：:Cationic spherical polyelectrolyte brushes (SPBs) were synthesized by photoemulsion polymerization, consisting of a polystyrene core with a diameter around 80 nm and a poly(2-aminoethylmethacrylate hydrochloride) (PAEMH) shell with a thickness from 10 to 50 nm densely grafted on the core surface. The binding of variou...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301865g
更新日期：2013-03-11 00:00:00
abstract：:Copolymers of (R)-3-hydroxybutyric acid (HB) and epsilon-caprolactone (CL) with a composition ranging from 28 to 81 mol % of HB were synthesized by transesterification of the corresponding homopolymers in solution in the presence of 4-toluenesulfonic acid. The copolyesters were characterized with regard to their molec...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025525t
更新日期：2002-07-01 00:00:00
abstract：:F-actins are semi-flexible polyelectrolytes and can be assembled into a large polymer-actin complex with polymorphism through electrostatic interaction with polycations. This study investigates the structural phase behavior and the growth of polymer-actin complexes in terms of its longitudinal and lateral sizes in var...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050320g
更新日期：2005-11-01 00:00:00
abstract：:A combination of chemo-photodynamic therapy has been manifested as a promising strategy for efficient cancer treatment due to the enhanced therapeutic efficacy. Here, we designed doxorubicin (DOX)-loaded photoresponsive micelles (DPRMs) based on a combination of chlorin e6 (Ce6) and lipoic acid (LA) conjugated methoxy...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00607
更新日期：2018-08-13 00:00:00
abstract：:In this work, we have analyzed the structural, topographical, and surface dilatational characteristics of pure beta-lactoglobulin adsorbed films and the effect of the self-assembly of monoglycerides (monopalmitin or monoolein) in beta-lactoglobulin films at the air-water interface. Measurements were performed in a sin...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060144r
更新日期：2006-09-01 00:00:00
abstract：:Repifermin, a truncated form of fibroblast growth factor-10 (FGF-10) also known as keratinocyte growth factor-2 (KGF-2), is a heparin-binding protein with potent regenerative properties. The protein unfolds and aggregates at relatively low temperature (~37 °C). Electrostatic interactions between polyanions and several...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2007967
更新日期：2011-11-14 00:00:00
abstract：:We created the dual-responsive nanovehicle that can effectively combine and abundantly utilize magnetic and glutathione (GSH)-reductive triggers to control the drug delivery and achieve more intelligent and powerful targeting. In the nanovehicles, paramagnetic fullerene (C60@CTAF) was prepared via one-step modificatio...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01939
更新日期：2017-03-13 00:00:00
abstract：:A drying environment is always a proposition faced by dynamic living organisms using water, which are driven by biopolymer-based micro- and macrostructures. Here, we introduce a drying process for aqueous liquid crystalline (LC) solutions composed of biopolymer with extremely high molecular weight components such as p...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00302
更新日期：2016-06-13 00:00:00
abstract：:High molecular weight trimethylene carbonate (TMC) and epsilon-caprolactone (CL) (co)polymers were synthesized. Melt pressed (co)polymer films were cross-linked by gamma irradiation (25 kGy or 50 kGy) in vacuum, yielding gel fractions of up to 70%. The effects of copolymer composition and irradiation dose on the cytot...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8007988
更新日期：2008-11-01 00:00:00
abstract：:Peptide self-assembly is a facile route to the development of bioorganic hybrid materials that have sophisticated nanostructures toward diverse applications. Here, we report the synthesis of self-assembled peptide (Fmoc-diphenylalanine, Fmoc-FF)/graphitic carbon nitride (g-C3N4) hydrogels for light harvesting and biom...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00889
更新日期：2017-11-13 00:00:00
abstract：:Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus , with a virus-like caged structure, has drawn much attention for its potential application as...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201587q
更新日期：2012-03-12 00:00:00
abstract：:Minimization of drug side effects is a hallmark of advanced targeted therapy. Herein we describe the synthesis of polysaccharide-based nanocapsules prepared from furcellaran and chitosan via layer-by-layer deposition using electrostatic interaction. Using doxorubicin as a model drug, prepared nanocapsules showed excel...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01175
更新日期：2020-02-10 00:00:00
abstract：:A three-dimensional structure consisting of poly(ε-caprolactone) (PCL) nanofibers covered by periodically spaced PCL crystal lamellae, a self-induced nanohybrid shish-kebab (SINSK) structure, was created using electrospinning followed by a self-induced crystallization. The resulting structure that resembles the nanoto...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400928b
更新日期：2013-10-14 00:00:00
abstract：:The synthesis and polymerization of six AB(3) tapered self-assembling methacrylate monomers (5a, 5b, 5c,5d, 17a, and 17b) based on first generation alkyl substituted benzyl ether monodendrons (i.e., minidendrons) containing oligooxyethylene units at their focal point and the polymerizable group on their periphery are ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010138p
更新日期：2002-01-01 00:00:00
abstract：:This paper reports the synthesis of hyaluronan (HA) and its derivatives via the hyaluronidase-catalyzed polymerization of 2-substituted oxazoline derivative monomers designed as "transition-state analogue substrates". Polymerization of 2-methyl oxazoline monomer from N-acetylhyalobiuronate (GlcAbeta(1-->3)GlcNAc) effe...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049280r
更新日期：2005-03-01 00:00:00
abstract：:Amphiphilic block copolymer self-assembly provides a versatile means to prepare nanoscale micelles in solution. The utilization of these structures as targeted drug delivery vehicles has motivated efforts to prepare bioactive ligand-functionalized polymer micelles. The impact of ligand conjugation on micelle morpholog...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900149b
更新日期：2009-06-08 00:00:00
abstract：:Nucleic acids have been engineered to participate in a wide variety of tasks. Among them, the enzyme-free amplification modes, enzyme-free DNA circuits (EFDCs), and hybridization chain reactions (HCRs) have been widely applied in a series of studies of bioanalysis. We demonstrated here an ultrasensitive hairpin probe-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00959
更新日期：2015-11-09 00:00:00
abstract：:Nanoparticles (NPs) composed of chitosan (CS) and poly(gamma-glutamic acid) (gamma-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and gamma-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crys...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0607776
更新日期：2007-01-01 00:00:00
abstract：:Cardiovascular diseases plague human health because of the lack of transplantable small-diameter blood vessel (SDBV) grafts. Although expanded polytetrafluoroethylene (ePTFE) has the potential to be used as a biocompatible material for SDBV grafts, long-term patency is still the biggest challenge. As discussed in this...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00897
更新日期：2020-09-14 00:00:00
abstract：:The growing importance of biodegradable conducting polymers (CPs) have fueled the rapid development of this unique class of polymeric materials in recent years. Possessing both the electrical conductivity approaching those of metallic conductors and the biodegradability of biocompatible polymers, biodegradable CPs are...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.8b00275
更新日期：2018-06-11 00:00:00
abstract：:meso-Tetra(N-methyl-4-pyridyl) porphine tetra tosylate (TMP) is a photosensitizer that can be used in photodynamic therapy (PDT) to induce cell death through generation of reactive oxygen species in targeted tumor cells. However, TMP is highly hydrophilic, and therefore, its ability to accumulate intracellularly is li...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301858a
更新日期：2013-02-11 00:00:00
abstract：:The SEC-MALS-QELS (size-exclusion chromatography equipped with multiangle light scattering and quasi-elastic light scattering detectors) method using lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) and LiCl/1,3-dimethyl-2-imidazolidinone (LiCl/DMI) as mobile phases was applied to cellulose and cellulose tricarbanil...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049386m
更新日期：2005-05-01 00:00:00
abstract：:Recently, we have developed a photopolymerizable poly(L-lysine) (PLL) that can be covalently incorporated into poly(ethylene glycol) diacrylate (PEGDA) hydrogels to improve their bioactivity by providing positive charges. To explore the potential of these PLL-grafted PEGDA hydrogels as a cell delivery vehicle and lumi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300381d
更新日期：2012-05-14 00:00:00
abstract：:The ability of microbally produced poly(gamma,d-glutamic acid) to form stable polyelectrolyte-opposite charged surfactant complexes was investigated. A sonicated sample of polyacid with a molecular weight about 10(5) Da and a content of d enantiomer higher than 90% was used in this study. Nearly stoichiometric complex...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034267c
更新日期：2004-01-01 00:00:00
abstract：:Poly(ethylene 2,5-furandicarboxylate) (PEF) is a biobased polyester characterized by high gas barrier properties as well as high tensile modulus and strength, but poor toughness. Toughening PEF without sacrificing its modulus, strength and gas barrier performance is a great challenge for PEF modification. In this stud...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01495
更新日期：2019-01-14 00:00:00
abstract：:C13-dipeptides that did not gel on their own were found to form hydrogels when combined with mixtures (coassembly). At pH = 4.6, by mixing negatively charged C13-WD (C13-WD2- and/or C13-WD-) with C13-KW or C13-YK, where the side chain of K carried positive charge, two composite hydrogels with different mechanical prop...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01325
更新日期：2020-12-14 00:00:00
abstract：:Liver glycogen, a highly branched polymer of glucose, is important for maintaining blood-glucose homeostasis. It was recently shown that db/db mice, a model for Type 2 diabetes, are unable to form the large composite glycogen α particles present in normal, healthy mice. In this study, the structure of healthy mouse-li...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401714v
更新日期：2014-02-10 00:00:00
