Abstract:
:meso-Tetra(N-methyl-4-pyridyl) porphine tetra tosylate (TMP) is a photosensitizer that can be used in photodynamic therapy (PDT) to induce cell death through generation of reactive oxygen species in targeted tumor cells. However, TMP is highly hydrophilic, and therefore, its ability to accumulate intracellularly is limited. In this study, a strategy to improve TMP uptake into cells has been investigated by encapsulating the compound in a hydrogel-based chitosan/alginate nanoparticle formulation. Nanoparticles of 560 nm in diameter entrapping 9.1 μg of TMP per mg of formulation were produced and examined in cell-based assays. These particles were endocytosed into human colorectal carcinoma HCT116 cells and elicited a more potent photocytotoxic effect than free drug. Antibodies targeting death receptor 5 (DR5), a cell surface apoptosis-inducing receptor up-regulated in various types of cancer and found on HCT116 cells, were then conjugated onto the particles. The conjugated antibodies further enhanced uptake and cytotoxic potency of the nanoparticle. Taken together, these results show that antibody-conjugated chitosan/alginate nanoparticles significantly enhanced the therapeutic effectiveness of entrapped TMP. This novel approach provides a strategy for providing targeted site-specific delivery of TMP and other photosensitizer drugs to treat colorectal tumors using PDT.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Abdelghany SM,Schmid D,Deacon J,Jaworski J,Fay F,McLaughlin KM,Gormley JA,Burrows JF,Longley DB,Donnelly RF,Scott CJdoi
10.1021/bm301858asubject
Has Abstractpub_date
2013-02-11 00:00:00pages
302-10issue
2eissn
1525-7797issn
1526-4602journal_volume
14pub_type
杂志文章abstract::A new family of cationic dendron-bearing lipids (CDLs) with poly(amidoamine) dendrons of first to third generation (named as A1, A2, and A3, respectively) was synthesized through a synthesis approach that permits facile variation of chemical structures. All CDLs could effectively bind small interfering RNA (siRNA) to ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm4011563
更新日期:2013-12-09 00:00:00
abstract::Plastics ending up in soil or landfill environment will eventually be degraded. At the same time a slow migration of additives from the plastics will occur. The additives and the degradation products of these and the polymer are potential compounds for bioconversion. To predict the rate of the migration of antioxidant...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0000283
更新日期:2000-10-01 00:00:00
abstract::Despite the wide interest raised by lung administration of nanoparticles (NPs) for the treatment of various diseases, little information is available on their effect toward the airway epithelial barrier function. In this study, the potential damage of the pulmonary epithelium upon exposure to poly(lactide-co-glycolide...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201226x
更新日期:2011-11-14 00:00:00
abstract::Spiders have evolved a complex system of silk producing glands. Each of the glands produces silk with strength and elasticity tailored to its biological purpose. Sequence analysis of the major ampullate silk reveals four highly conserved concatenated blocks of amino acids: (GA) n , A n , GPGXX, and GGX. While the GPGX...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701124p
更新日期:2008-06-01 00:00:00
abstract::The intermolecular distributions of amide groups within two commercial LMA pectins was studied after removal of the methyl esters followed by fractionation of the different populations by anion exchange chromatography. The populations obtained had almost equal degrees of amidation while the values of the degree of blo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050960j
更新日期:2006-06-01 00:00:00
abstract::Numerous catechol-containing polymers, including biodegradable polymers, are currently heavily discussed for modern biomaterials. However, there is no report combining poly(phosphoester)s (PPEs) with catechols. Adhesive PPEs have been prepared via acyclic diene metathesis polymerization. A novel acetal-protected catec...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01613
更新日期:2017-03-13 00:00:00
abstract::The supramolecular organization of self-assembled stoichiometric complexes formed by dodecyltrimethylammonium cations and ionized poly(gamma-glutamic) acid has been investigated in chloroform solution. Atomistic molecular dynamics simulations have been performed considering three different starting conformations for t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060927c
更新日期:2007-02-01 00:00:00
abstract::A well-defined N-(2-hydroxypropyl)methacrylamide-s-N-(3-aminopropyl)methacrylamide (HPMA-s-APMA) copolymer, synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization, was utilized for the rational design of multiconjugates containing both a gene therapeutic, small interfering RNA (siRNA), ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901249n
更新日期:2010-02-08 00:00:00
abstract::As a first step toward utilizing self-assembling peptide scaffolds to create tunable matrices for drug delivery, peptide RADAFI and RADAFII, containing the same amino acid composition but different positions of one phenylalanine residue, scaffolds were prepared for controlled release of chiral enantiomers. The release...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900857j
更新日期:2009-12-14 00:00:00
abstract::Strong signaling cascades derived from upregulation and overexpression of growth factors such as the EGF-family (epidermal growth factors) have been crucially related to cancer pathogenesis. Gene silencing techniques to modulate the expression of oncogenes and tumor suppresor genes are a strategy that shows great prom...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01066
更新日期:2018-10-08 00:00:00
abstract::Polymer conjugation increases an enzyme's circulation time and stability for use as a therapeutic agent, but this attachment indubitably affects its properties. Covalent attachment of multiple polyethylene glycol chains with sizes of either 2, 5, 10, or 20 kDa increases the molecular weight and hydrodynamic radius of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1006954
更新日期:2010-12-13 00:00:00
abstract::Bioresponsive polymers can enable the development of more effective drug delivery vehicles and medical materials. Acetal-modified polysaccharides allow pH-triggered solubility switching in a versatile and effective manner, but prior work has been limited to a combination of acyclic alkoxyisopropyl and cyclic isopropyl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00215
更新日期:2019-05-13 00:00:00
abstract::The unsatisfied results of cancer therapy are caused by many issues and metastasis of cancer cells is one of the major challenge. It has been reported that inhibiting the SDF1/CXCR4 interaction can significantly reduce the metastasis of breast cancer cells to regional lymph nodes and lung. Herein, a nanogel system equ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00208
更新日期:2017-06-12 00:00:00
abstract::Effects of molecular weight (Lc) and field strength (E) on conformation of polymer chains and their density are investigated at the surface and bulk of a driven chain system in three dimensions with a Monte Carlo simulation. As the polymer chains deposit on the surface, a linear density gradient develops in low field....
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm000289c
更新日期:2000-10-01 00:00:00
abstract::Weissella confusa VTT E-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked D-glucosyl units and very few α-(1→3) branch linkages. A mixture of the Chaetomium erraticum endodextranase and the Aspergillus niger α-glucosidase was used to hydrolyze W. confusa dextran to glucose and a set...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1011536
更新日期:2011-02-14 00:00:00
abstract::The first step in the conventional approach to self-assembled biomaterials is to develop well-defined nanostructures in vitro, which is followed by disruption of the preformed nanostructures at the inside of the cell to achieve bioactivity. Here, we propose an inverse strategy to develop in-cell gain-of-function self-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01816
更新日期:2017-03-13 00:00:00
abstract::Synthesis of novel zwitterionic block copolypeptides, poly(N-isopropylacrylamide)-block-poly(L-glutamic acid-co-L-lysine) [PNiPAM(n)(PLG(x)-co-PLLys(y))m , where n is the number-average degree of polymerization (DP(n)) of PNiPAM block, x and y are the mole fraction of glutamic acid and lysine residues, respectively, a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800394p
更新日期:2008-10-01 00:00:00
abstract::Atomic force microscopy (AFM) was used to assess a new chimeric protein consisting of a fusion protein of the consensus repeat for Nephila clavipes spider dragline protein and bone sialoprotein (6mer+BSP). The elastic modulus of this protein in film form was assessed through force curves, and film surface roughness wa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2000605
更新日期:2011-05-09 00:00:00
abstract::Relative to normal cells, tumor cells lack adequate capability of reactive oxygen scavenging. Thus, tumor cells can be selectively killed by increasing the concentration of reactive oxygen species in tumor tissue. In this report, we construct an integrated multifunctional polymeric nanoparticle which can selectively i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01777
更新日期:2018-06-11 00:00:00
abstract::Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm070062i
更新日期:2007-09-01 00:00:00
abstract::The proteinaceous material present in beta-lactoglobulin fibrils formed after heating (20 h at 85 degrees C) at pH 2 was identified during this study. Fibrils were separated from the nonaggregated material, and the fibrils were dissociated using 8 M guanidine chloride and 0.1 M 1,4-dithiothreitol (pH 8). Characterizat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7014224
更新日期:2008-05-01 00:00:00
abstract::Natural melanins are biocompatible conductors with versatile functionalities. Here, we report fabrication of multifunctional poly(vinyl alcohol)/melanin nanocomposites by layer-by-layer (LBL) assembly using melanin nanoparticles (MNPs) directly extracted from sepia officinalis inks. The LBL assembly offers facile mani...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00336
更新日期:2017-06-12 00:00:00
abstract::A shortage of available organ donors has created a need for engineered tissues. In this context, polymer-based hydrogels that break down inside the body are often used as constructs for growth factors and cells. Herein, we report imine cross-linked gels where degradation is controllable by the introduction of mixed im...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00519
更新日期:2015-07-13 00:00:00
abstract::In situ forming reduction-sensitive degradable nanogels were designed and developed based on poly(ethylene glycol)-b-poly(2-(hydroxyethyl) methacrylate-co-acryloyl carbonate) (PEG-P(HEMA-co-AC)) block copolymers for efficient loading as well as triggered intracellular release of proteins. PEG-P(HEMA-co-AC) copolymers ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400206m
更新日期:2013-04-08 00:00:00
abstract::Aggregation phenomena in aqueous solutions of purified human tracheobronchial mucin have been studied by rheological methods, steady-state fluorescence, quasielastic light scattering, and spin probe techniques. At temperatures below 30 degrees C and concentrations above 15 mg/mL and in the absence of chaotropic agents...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005532m
更新日期:2000-10-01 00:00:00
abstract::Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100138m
更新日期:2010-06-14 00:00:00
abstract::The manufacture of biodegradable materials from agricultural sources is a real challenge, because of environmental concerns and the need to make full use of resources. Cottonseed is an important protein source that could be used in nonfood applications, as a substitute for synthetic polymers. For the first time, the v...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm015525d
更新日期:2001-01-01 00:00:00
abstract::Turbidimetric titration was used to initiate associative intermolecular interactions between a pair of protein molecules, gelatin-A and gelatin-B, having complementary charges that led to pH-induced liquid-liquid phase separation and the formation of complex coacervate. The stoichiometric binding ratio was found to be...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801160s
更新日期:2009-01-12 00:00:00
abstract::An appropriate modification technique for silk materials is needed to effectively improve their physical properties for specific applications. A telechelic-type polyalanine (T-polyA) was synthesized by papain-catalyzed polymerization as a novel reinforcing agent for silk materials. A silk fibroin obtained from Bombyx ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01891
更新日期:2017-03-13 00:00:00
abstract::Binding interactions between low molecular weight heparin (LMWH) and heparin-binding peptides (HBP) have been applied as a strategy for the assembly of hydrogels that are capable of sequestering growth factors and delivering them in a controlled manner. In this work, the assembly of four-arm star poly(ethylene glycol)...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0500042
更新日期:2005-07-01 00:00:00