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High level biosynthesis of a silk-elastin-like protein in E. coli.

Abstract：

:Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications, yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L(-1) with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high preinduction growth rates and low inducer and oxygen concentrations are allowed whereas reduced postinduction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible, and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Collins T,Barroca M,Branca F,Padrão J,Machado R,Casal M

doi

10.1021/bm5005564

subject

Has Abstract

pub_date

2014-07-14 00:00:00

pages

2701-8

issue

7

eissn

1525-7797

issn

1526-4602

journal_volume

15

pub_type

杂志文章

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