Abstract：
:Biomimetic nanoengineering built through integrating the specific cell membrane with artificially synthetic nanomedicines represents one of the most promising directions for the actualization of personalized therapy. For addressing the technical hurdle against the development of this biomimetic technology, the present report describes the in-depth exploration and optimization over each critical preparation step, including establishment of a nanoparticle-stabilized dispersion system, cargo loading, membrane coating, and product isolation. Magnetic iron oxide nanoparticles loaded with DOX is used as a typical model for the coating with cancer cell membranes, providing compact DNP@CCCM nanostructure well-characterized by various techniques. Furthermore, the feasibility of this optimized approach in constructing biomimetic membrane-coated nanomedicines has been validated on the basis of the remarkably improved biofunctions, such as the targetability, magnetic property, hemolysis risk, macrophage evasion, in vitro cytotoxicity, in vivo circulation duration, and in vivo principal component analysis postinjection. We hope this study regarding technique optimization will prompt the advancement of biomembrane-camouflaged nanoparticles as a newly emerging biomimetic technology.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Zhu J,Zhang M,Zheng D,Hong S,Feng J,Zhang XZdoi
10.1021/acs.biomac.8b00242subject
Has Abstractpub_date
2018-06-11 00:00:00pages
2043-2052issue
6eissn
1525-7797issn
1526-4602journal_volume
19pub_type
杂志文章abstract：:Electrospun natural polymer membranes were fabricated from collagen or gelatin coated with a bioactive recombinant fragment of perlecan, a natural heparan sulfate proteoglycan. The electrospinning process allowed the facile processing of a three-dimensional, porous fibril (2-6 microm in diameter) matrix suitable for t...
journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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更新日期：2002-03-01 00:00:00
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更新日期：2005-01-01 00:00:00
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更新日期：2018-11-12 00:00:00
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