Abstract:
:Multicomponent poly(ethylene glycol) (PEG) brushes (i.e., ≥ 2 adjacent PEG brushes) can be used to engineer culture substrates with microscale, nonfouling regions decorated with covalently immobilized ligands that mediate biospecific interactions. However, synthesizing such brushes with orthogonal immobilization chemistries to permit differential biofunctionalization is nontrivial and often requires synthesis of PEG-co-polymers. To simplify synthesis and enhance the versatility of such substrates, we developed a protocol for generating orthogonal click-functionalized multicomponent PEG brushes using sequential nucleophilic substitutions by sodium azide, ethanolamine, and propargylamine. The novel application of propargylamine-mediated substitution functionalizes PEG brushes with acetylene groups, and for the first time, ethanolamine-mediated substitution is shown to be sufficient for passivating the "living" polymer chain ends between brush synthesis steps. Thus, our multicomponent PEG brushes present dual orthogonal chemistries (i.e., azido and acetylene groups) for ligand immobilization via versatile copper-free click reactions, which are useful for in situ surface modifications during cell culture.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Sha J,Lippmann ES,McNulty J,Ma Y,Ashton RSdoi
10.1021/bm400900rsubject
Has Abstractpub_date
2013-09-09 00:00:00pages
3294-303issue
9eissn
1525-7797issn
1526-4602journal_volume
14pub_type
杂志文章abstract::A wool fiber sample was submitted to chemical-free steam explosion in view of potential exploitation of keratin-based industrial and farm wastes. Fiber keratin was converted into a dark-yellow sludge that was submitted to phase separation by filtration, centrifugation, and precipitation of the soluble materials from t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060597w
更新日期:2006-12-01 00:00:00
abstract::The antiproliferative activity of alkylated heparin, in which the terminal end of heparin is derivatized with an alkyl group (butyl, octyl, lauryl, stearyl), was examined using vascular smooth muscle cells. The proliferation of cells, which were growth-arrested prior to addition of heparin, was inhibited in proportion...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm010097x
更新日期:2001-01-01 00:00:00
abstract::Neutral cellulose nanocrystals dispersed in water were shown in a previous work to stabilize oil/water interfaces and produce Pickering emulsions with outstanding stability, whereas sulfated nanocrystals obtained from cotton did not show interfacial properties. To develop a better understanding of the stabilization me...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201599j
更新日期:2012-01-09 00:00:00
abstract::Multifunctional and highly biocompatible polyether structures play a key role in shielding liposomes from degradation in the bloodstream, providing also multiple functional groups for further attachment of targeting moieties. In this work hyperbranched polyglycerol ( hbPG) bearing lipids with long alkyl chain anchor a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00115
更新日期:2018-07-09 00:00:00
abstract::The present study reports the folic acid (FA) functionalized insulin loaded stable liposomes with improved bioavailability following oral administration. Liposomes were stabilized by alternating coating of negatively charged poly(acrylic acid) (PAA) and positively charged poly(allyl amine) hydrochloride (PAH) over lip...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401580k
更新日期:2014-01-13 00:00:00
abstract::In the cell nucleus, putrescine, spermidine, and spermine self-assemble with phosphate ions to generate three forms of compounds, named nuclear aggregates of polyamines (NAPs), which may interact with DNA. In an in vitro setting mimicking the cell nucleus milieu, this molecular aggregation occurs within well-defined r...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101478j
更新日期:2011-04-11 00:00:00
abstract::Amino groups were covalently introduced onto a polycaprolactone (PCL) surface by the reaction between 1,6-hexanediamine and the ester groups of PCL. The occurrence of the aminolysis and the introduction of free NH(2) groups were verified qualitatively by fluorescence spectroscopy, where rhodamine B isothiocyanate was ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm020074y
更新日期:2002-11-01 00:00:00
abstract::G-quadruplex is a type of DNA secondary structure formed by specific guanine-rich sequences. Because of their enrichment in functional genomic regions and their biological significance, G-quadruplexes are recognized as significant drug targets for cancer and other diseases. Here, we tested the precipitation efficiency...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100862k
更新日期:2010-12-13 00:00:00
abstract::Injectable gels that support load are desirable for restoring the mechanical properties of degenerated load-bearing tissue. As these gels become increasingly sophisticated, the need to remotely image them and monitor their swelling increases. However, imaging such gels and monitoring their swelling using noninvasive m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00463
更新日期:2019-07-08 00:00:00
abstract::RNA-based delivery system for cancer therapy remains a challenge. In this study, a stearyl-peptide (SHR) was synthesized using arginine, histidine, cysteine, and stearyl moieties. Further, the stearyl-peptides were cross-linked by disulfide bonds to obtain cross-linked polypeptides (SHRss) with different molecular wei...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501777a
更新日期:2015-04-13 00:00:00
abstract::Amphiphilic block copolymer self-assembly provides a versatile means to prepare nanoscale micelles in solution. The utilization of these structures as targeted drug delivery vehicles has motivated efforts to prepare bioactive ligand-functionalized polymer micelles. The impact of ligand conjugation on micelle morpholog...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900149b
更新日期:2009-06-08 00:00:00
abstract::Tumorigenic cell behaviors can be suppressed or enhanced by their physicochemical environment. As a first step toward developing materials that allow tumorigenic behaviors to be observed and manipulated, we cultured related MCF10 breast cell lines on fibers composed of the Drosophila protein Ultrabithorax (Ubx). These...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01311
更新日期:2016-11-14 00:00:00
abstract::Electrospun poly(glycolide-co-lactide) (PLA10GA90, LA/GA ratio 10/90) biodegradable nanofiber membranes possessed very high surface area to volume ratios and were completely noncrystalline with a relatively lowered glass transition temperature. These characteristics led to very different structure, morphology, and pro...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025717o
更新日期:2003-03-01 00:00:00
abstract::Poly(lactide)-block-poly(ethylene oxide)-block-poly(lactide) (PLA-PEO-PLA) triblock copolymers are known to form physical hydrogels in water as a result of the polymer's amphiphilicity. Their mechanical properties, biocompatibility, and biodegradability have made them attractive for use as soft tissue scaffolds. Howev...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800557r
更新日期:2008-10-01 00:00:00
abstract::Branched amphiphilic copolymers were synthesized through the reversible addition-fragmentation chain transfer (RAFT) chain extension of a poly(methyl acrylate) macro-chain transfer agent using a protected galactose monomer and a polymerizable chain transfer agent branching unit. After galactose deprotection, the copol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501482q
更新日期:2015-01-12 00:00:00
abstract::Mammalian synovial joints are extremely efficient lubrication systems reaching friction coefficient μ as low as 0.001 at high pressures (up to 100 atm) and shear rates (up to 10(6) to 10(7) Hz); however, despite much previous work, the exact mechanism responsible for this behavior is still unknown. In this work, we st...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2004912
更新日期:2011-10-10 00:00:00
abstract::Consensus motifs for sequences of both crystallizable and amorphous blocks in silks and natural structural analogues of silks vary widely. To design novel silklike polypeptides, an important question is therefore how the nature of either the crystallizable or the amorphous block affects the self-assembly and resulting...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00512
更新日期:2019-10-14 00:00:00
abstract::Turbidimetric titration was used to initiate associative intermolecular interactions between a pair of protein molecules, gelatin-A and gelatin-B, having complementary charges that led to pH-induced liquid-liquid phase separation and the formation of complex coacervate. The stoichiometric binding ratio was found to be...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801160s
更新日期:2009-01-12 00:00:00
abstract::We studied methoxy poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PCL-b-PDMAEMA) nanoparticles as the codelivery vector of hydrophobic drug and pDNA by employing dynamic light scattering (DLS), ζ potential, transmission electron microscopy (TEM), gel retardation assay, a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100410m
更新日期:2010-09-13 00:00:00
abstract::X-ray analytical techniques are increasingly being used to study manuscripts and works of art on paper, whether with laboratory equipment or synchrotron sources. However, it is difficult to anticipate the impact of X-ray photons on paper- and cellulose-based artifacts, particularly due to the large variety of their co...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00512
更新日期:2020-07-13 00:00:00
abstract::Reductively degradable biopolymers have emerged as a unique class of smart biomedical materials. Here, a functional coupling agent, cystamine diisocyanate (CDI), was designed to offer a facile access to reductively degradable biopolymers via polycondensation with various diols. CDI was readily obtained with a decent y...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01578
更新日期:2016-03-14 00:00:00
abstract::Immunological detection has been developed into a sensitive and versatile technique and is based on two requisite elements: targeting antibodies and an indicator, usually in the form of horseradish peroxidase or alkaline phosphatase. The specificity and turnover rate of these enzymes provide an efficient means of sign...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401511a
更新日期:2014-01-13 00:00:00
abstract::Poly(beta-hydroxybutyrate) (PHB) modified with different amounts of pentaerythritol ethoxylate (PEE) has been synthesized using Ralstonia eutropha. The growth kinetics and the synthesis of PHB in the presence of PEE were modeled using appropriate differential equations for the mass balance of the two-stage process. Th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm010088o
更新日期:2001-10-01 00:00:00
abstract::On the basis of their versatile structure and chemistry as well as tunable mechanical properties, polymer brushes are well-suited as supports for enzyme immobilization. However, a robust surface design is hindered by an inadequate understanding of the impact on activity from the coupling motif and enzyme distribution ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200173y
更新日期:2011-05-09 00:00:00
abstract::Despite recent advances in tumor treatment through cancer immunotherapy, the efficacy of this approach remains to be improved. Looking forward to high rates of objective clinical response, cancer immunotherapy combined with chemotherapy has gained increasing attention recently. Here, we constructed liposomes with matr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01510
更新日期:2019-02-11 00:00:00
abstract::Reversible stereoselective complexes were spontaneously obtained from mixing acetonitrile solutions of enatiomeric d-poly(lactic acid) (d-PLA), l-poly(lactic acid) (l-PLA), and leuprolide, a l-configured nonapeptide LHRH analogue. The complex spontaneously aggregated and precipitated in high yields (>90%) from acetoni...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0300249
更新日期:2003-09-01 00:00:00
abstract::Bioresponsive poly(amidoamine)s (PAA)s are currently under development as endosomolytic polymers for intracellular delivery of proteins and genes. Here for the first time, small-angle neutron scattering (SANS) is used to systematically investigate the pH-dependent conformational change of an endosomolytic polymer, the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049936g
更新日期:2004-07-01 00:00:00
abstract::Gelatin microspheres are well-known for their capacity to release growth factors in a controlled manner, but gelatin microspheres do not calcify in the absence of so-called bioactive substances that induce deposition of calcium phosphate (CaP) bone mineral. This study has investigated if CaP nanocrystals can be incorp...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1006344
更新日期:2010-10-11 00:00:00
abstract::We have investigated the thermodynamic and dynamic behavior of multistranded β-lactoglobulin protein fibrils in water, by combining static, dynamic, and depolarized dynamic light scattering (SLS, DLS, DDLS), small angle neutron scattering (SANS), rheology, and cryogenic transmission electron microscopy (cryo-TEM). We ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301005w
更新日期:2012-10-08 00:00:00
abstract::Hydrogels are able to mimic the basic three-dimensional (3D) biological, chemical, and mechanical properties of native tissues. Since hyaluronic acid (HA) is a chief component of human extracellular matrix (ECM), it represents an extremely attractive starting material for the fabrication of scaffolds for tissue engine...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401712q
更新日期:2014-02-10 00:00:00