Abstract:
:A lipid bilayer was deposited on a 3 μm diameter polystyrene (PS) bead via hydrophobic anchoring of bicelles containing oxyamine-bearing cholesteric moieties reacting with the aldehyde functionalized bead surface. Discoidal bicelles were formed by mixing dimyristoylphosphatidylcholine (DMPC), dihexanoylphosphatidylcholine (DHPC), dimyristoyltrimethylammonium propane (DMTAP), and the oxyamine-terminated cholesterol derivative, cholest-5-en-3β-oxy-oct-3,6-oxa-an-8-oxyamine (CHOLOA), in the molar ratio DMPC/DHCP/DMTAP/CHOLOA (1/0.5/0.01/0.05) in water. Upon exposure to aldehyde-bearing PS beads, a stable single lipid bilayer coating rapidly formed at the bead surface. Fluorescence recovery after photobleaching demonstrated that the deposited lipids fused into an encapsulating lipid bilayer. Electrospray ionization mass spectrometry showed that the short chain lipid DHPC was entirely absent from the PS adherent lipid coating. Fluorescence quenching measurements proved that the coating was a single lipid bilayer. The bicelle coating method is thus simple and robust, can be modified to include membrane-associated species, and can be adapted to coat any number of different surfaces.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Saleem Q,Zhang Z,Petretic A,Gradinaru CC,Macdonald PMdoi
10.1021/acs.biomac.5b00042subject
Has Abstractpub_date
2015-03-09 00:00:00pages
1032-9issue
3eissn
1525-7797issn
1526-4602journal_volume
16pub_type
杂志文章abstract::Deproteinized natural rubber latex (DPNR-latex) was treated with lipase and phosphatase in order to analyze the structure of the chain-end group (alpha-terminal). The enzymatic treatment decreased the content of long-chain fatty acid ester groups in DPNR from about 6 to 2 mol per rubber molecule. The molecular weight ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm058003x
更新日期:2005-07-01 00:00:00
abstract::Eosinophil cationic protein (ECP) is an antimicrobial protein belonging to the superfamily of RNase A. ECP exhibits a broad spectrum of action against bacteria and, at higher concentrations, displays cytotoxic activity to eukaryotic cells. Recently, a powerful aggregation activity for lipid vesicles and for the gram-n...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100334u
更新日期:2010-08-09 00:00:00
abstract::Recombinant spider silk proteins mimicking the properties of dragline silk proteins represent a class of materials that hold great potential for future high-performance applications. Here we explore the self-assembly behavior of a recombinantly produced spider silk protein based on the dragline silk of the Araneus di...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300964h
更新日期:2012-10-08 00:00:00
abstract::Owing to their diverse functions and tunable physicochemical properties, peptides are promising alternatives to the conventional gene delivery tools that are available for plant systems. However, peptide-mediated gene delivery is limited by low transfection efficiency in plants because of the insufficient cytosolic tr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00481
更新日期:2020-07-13 00:00:00
abstract::In the present study we describe the synthesis, wet spinning, mechanical testing, and degradation of poly(urethane urea)s (PUURs) intended for clinical use in anterior cruciate ligament (ACL) reconstruction. The effects of soft segment chemical composition and molar mass and the kind of diamine chain extender on the m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025535u
更新日期:2002-09-01 00:00:00
abstract::Recent years have seen rapid growth in utilizing vegetable oils to derive a wide variety of polymers to replace petroleum-based polymers for minimizing environmental impact. Nonedible castor oil (CO) can be extracted from castor plants that grow easily, even in an arid land. CO is a promising source for developing sev...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01291
更新日期:2020-12-14 00:00:00
abstract::Macromolecular dynamics in biological systems, which play a crucial role for biomolecular function and activity at ambient temperature, depend strongly on moisture content. Yet, a generally accepted quantitative model of hydration-dependent phenomena based on local relaxation and diffusive dynamics of both polymer and...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00191
更新日期:2018-07-09 00:00:00
abstract::A major challenge in drug delivery is the internalization through the apical plasma membrane of the polarized epithelial cells lining organs facing the external environment, e.g., lungs and the gastrointestinal tract. The reduced permeation of drugs entering through this pathway is in part due to the mucosal barrier a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700535p
更新日期:2008-02-01 00:00:00
abstract::Photoexcited TiO2 degrades biomolecules such as nucleic acids, cell membrane proteins, and enzymes. Stabilization of enzyme activity against the deactivation caused by the combination of TiO2-UV is essential if we are to develop novel hybrid materials exhibiting photocatalytic and biocatalytic activities useful for de...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049728o
更新日期:2004-09-01 00:00:00
abstract::The polarity of polymer-actin complexes obtained by mixing F-actin with synthetic polymers carrying positive charges such as poly(L-lysine), x,y-ionene bromide polymers, and poly(N-[3-(dimethylamino)propyl]acrylamide) (PDMAPAA-Q) have been investigated. Actin complexes formed with poly(L-lysine) and PDMAPAA-Q, which c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0494038
更新日期:2005-03-01 00:00:00
abstract::A novel pH-sensitive amphiphilic copolymer brush poly(methyl methacrylate-co-methacrylic acid)-b-poly(poly(ethylene glycol) methyl ether monomethacrylate) [P(MMA-co-MAA)-b-PPEGMA] was defined and synthesized by atom transfer radical polymerization (ATRP) technique. The molecular structures and characteristics of this ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101058w
更新日期:2011-01-10 00:00:00
abstract::A stepwise anticancer drug delivery system based on an injectable supramolecular hydrogel was presented. In this system, poly(ethylene glycol)-b-poly(acrylic acid) (PEG-b-PAA) block copolymer nanoparticles containing cisplatin were released by erosion of the hydrogels and then the cisplatin was released from the nanop...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100889j
更新日期:2010-11-08 00:00:00
abstract::Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm070062i
更新日期:2007-09-01 00:00:00
abstract::Poly(L-lactide) (PLLA) with various alkyl ester chain end groups were synthesized by ring-opening polymerization of L-lactide in the presence of zinc alkoxide as a catalyst. The structural effect of chain end groups on the rate of enzymatic and nonenzymatic degradations for amorphous films of PLLA were investigated at...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701259r
更新日期:2008-03-01 00:00:00
abstract::Click chemistry is a versatile tool for the synthesis and functionalization of polymeric biomaterials. Here, we describe a versatile new strategy for producing bioactive, protein-functionalized poly(ethylene glycol) (PEG) hydrogel microparticles that is based on sequential thiol-ene and tetrazine click reactions. Brie...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00990
更新日期:2016-11-14 00:00:00
abstract::The coefficient of thermal expansion (CTE) of cellulose nanocrystal (CNC) films was characterized using novel experimental techniques complemented by molecular simulations. The characteristic birefringence exhibited by CNC films was utilized to calculate the in-plane CTE of self-organized and shear-oriented self-stand...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400794e
更新日期:2013-08-12 00:00:00
abstract::Cellulose-based dendrimerized material was prepared and its quality was assessed by determining the number of amine functional groups incorporated. Based on the results for a series of preparations, the material was obtained in a highly reproducible manner thanks to the particular chemical construction method used. Th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701380a
更新日期:2008-05-01 00:00:00
abstract::We report the facile preparation of gels from the hydrothermal treatment of suspensions of cellulose nanocrystals (CNCs). The properties of the hydrogels have been investigated by rheology, electron microscopy, and spectroscopy with respect to variation in the temperature, time, and CNC concentration used in preparati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00906
更新日期:2016-08-08 00:00:00
abstract::Tissue engineering approaches need biomaterials with suitable properties to provide an appropriate environment for cell attachment and growth. The performance of these biomaterials can be greatly enhanced through the incorporation of bioactive agents. For this reason, we developed chitosan films with cell-attachment a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701049g
更新日期:2008-02-01 00:00:00
abstract::Control of the photoluminescence properties of cellulose is conducted by introduction of conducting polymers including fluorene (F) and 3-hexylthiophene (3HT) on cellulose surface through FeCl3 oxidative polymerization. The UV-vis absorption peak of cellulose grafted with the 3-hexylthiophene and fluorene copolymer wa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01067
更新日期:2017-12-11 00:00:00
abstract::A series of well-defined thermoresponsive diblock copolymers (PEO45-b-PtNEAn, n=22, 44, 63, 91, 172) were prepared by the atom transfer radical polymerization of trans-N-(2-ethoxy-1,3-dioxan-5-yl) acrylamide (tNEA) using a poly(ethylene oxide) (PEO45) macroinitiator. All copolymers are water-soluble at low temperature...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400180n
更新日期:2013-05-13 00:00:00
abstract::The complement system is a powerful mechanism of the innate immune defense system. Dysregulation may contribute to several diseases. Heparin is a known regulator of the complement system, but its application is limited due to its anticoagulative activity. A promising alternative is the synthetic analogue dendritic pol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00889
更新日期:2019-10-14 00:00:00
abstract::We present a new design of peptide-polymer conjugates where a polymer chain is covalently linked to the side chain of a helix bundle-forming peptide. The effect of conjugated polymer chains on the peptide structure was examined using a de novo designed three-helix bundle and a photoactive four-helix bundle. Upon attac...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800113g
更新日期:2008-08-01 00:00:00
abstract::The first step in the conventional approach to self-assembled biomaterials is to develop well-defined nanostructures in vitro, which is followed by disruption of the preformed nanostructures at the inside of the cell to achieve bioactivity. Here, we propose an inverse strategy to develop in-cell gain-of-function self-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01816
更新日期:2017-03-13 00:00:00
abstract::Materials with fungi-bioinspired surface have been designed to host ergosterol-binding polyene antibiotics and to release them via a competitive mechanism only when fungi are present in the medium. Silicone rubber (SR) surfaces were endowed with selective loading and fungi-triggered release of polyene antifungal agent...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500257s
更新日期:2014-05-12 00:00:00
abstract::Supramolecular nanogel, a physically cross-linked nanosize hydrogel, spontaneously self-assembles in aqueous solution via secondary interactions and is thus of great interest in nanomedicine as a drug carrier. We developed a versatile method for supramolecular nanogel self-assembled by electrostatic interaction betwee...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400846h
更新日期:2013-10-14 00:00:00
abstract::Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment. The starting suspensions used at ∼6 wt % CNC separated in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501629g
更新日期:2014-12-08 00:00:00
abstract::The conformational behavior of a long single-chain double-stranded DNA in solutions of free silver ions and silver nanoparticles generated via the reduction of AgNO3 by NaBH4 was monitored by fluorescence and electron microscopies and UV spectroscopy. The interaction of monovalent silver ions with DNA induces shrinkin...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800235j
更新日期:2008-07-01 00:00:00
abstract::Controlled reeling is a powerful tool to investigate the details of silk processing. However, consistent forced reeling of silkworms is hindered by the significant degree of behaviorally induced variation caused by the animal. This paper proposes silkworm paralysis as a novel method to control the animal and thus in v...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401013k
更新日期:2013-10-14 00:00:00
abstract::The basic physicochemical properties that determine the distribution and fate of synthetic macromolecules in living cells were characterized using fluorescently labeled HPMA (N-(2-hydroxypropyl)methacrylamide) copolymers. Twelve different classes of water-soluble copolymers were created by incorporating eight differen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801514x
更新日期:2009-07-13 00:00:00