Abstract：
:A series of well-defined thermoresponsive diblock copolymers (PEO45-b-PtNEAn, n=22, 44, 63, 91, 172) were prepared by the atom transfer radical polymerization of trans-N-(2-ethoxy-1,3-dioxan-5-yl) acrylamide (tNEA) using a poly(ethylene oxide) (PEO45) macroinitiator. All copolymers are water-soluble at low temperature, but upon quickly heating to 37 °C, laser light scattering (LLS) and transmission electron microscopy (TEM) characterizations indicate that these copolymers self-assemble into aggregates with different morphologies depending on the chain length of PtNEA and the polymer concentration; the morphologies gradually evolved from spherical solid nanoparticles to a polymersome as the degree of polymerization ("n") of PtNEA block increased from 22 to 172, with the formation of clusters with rod-like structure at the intermediate PtNEA length. Both the spherical nanoparticle and the polymersome are stable at physiological pH but susceptible to the mildly acidic medium. Acid-triggered hydrolysis behaviors of the aggregates were investigated by LLS, Nile red fluorescence, TEM, and (1)H NMR spectroscopy. The results revealed that the spherical nanoparticles formed from PEO45-b-PtNEA44 dissociated faster than the polymersomes of PEO45-b-PtNEA172, and both aggregates showed an enhanced hydrolysis under acidic conditions. Both the spherical nanoparticle and polymersome are able to efficiently load the hydrophobic doxorubicin (DOX), and water-soluble fluorescein isothiocyanate-lysozyme (FITC-Lys) can be conveniently encapsulated into the polymersome without using any organic solvent. Moreover, FITC-Lys and DOX could be coloaded in the polymersome. The drugs loaded either in the polymersome or in the spherical nanoparticle could be released by acid triggering. Finally, the DOX-loaded assemblies display concentration-dependent cytotoxicity to HepG2 cells, while the copolymers themselves are nontoxic.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Qiao ZY,Ji R,Huang XN,Du FS,Zhang R,Liang DH,Li ZCdoi
10.1021/bm400180nsubject
Has Abstractpub_date
2013-05-13 00:00:00pages
1555-63issue
5eissn
1525-7797issn
1526-4602journal_volume
14pub_type
杂志文章abstract：:To evaluate the antidiarrheal effect of ginseng, the active principals of ginseng were studied in vitro model of rotavirus infection, the leading cause of severe diarrhea. Two pectic polysaccharides, named as GP50-dHR (56.0 kDa) and GP50-eHR (77.0 kDa), were purified from hot water extract of ginseng by bioassay-linke...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100397p
更新日期：2010-08-09 00:00:00
abstract：:Binding interactions between low molecular weight heparin (LMWH) and heparin-binding peptides (HBP) have been applied as a strategy for the assembly of hydrogels that are capable of sequestering growth factors and delivering them in a controlled manner. In this work, the assembly of four-arm star poly(ethylene glycol)...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0500042
更新日期：2005-07-01 00:00:00
abstract：:The electrospinning of polymer melts can offer an advantage over solution electrospinning, in the development of layered tissue constructs for tissue engineering. Melt electrospinning does not require a solvent, of which many are cytotoxic in nature, and the use of nonwater soluble polymers allows the collection of fi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050777q
更新日期：2006-03-01 00:00:00
abstract：:Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049941k
更新日期：2004-07-01 00:00:00
abstract：:Photoexcited TiO2 degrades biomolecules such as nucleic acids, cell membrane proteins, and enzymes. Stabilization of enzyme activity against the deactivation caused by the combination of TiO2-UV is essential if we are to develop novel hybrid materials exhibiting photocatalytic and biocatalytic activities useful for de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049728o
更新日期：2004-09-01 00:00:00
abstract：:Hydrophobic methacrylic monomers were polymerized in aqueous media using methylated (1.8)-beta-cyclodextrin (MeCD) additives. Hydrophobic monomers tert-butyl methacrylate (tBuMA), cyclohexyl methacrylate (CMA), and 2-ethylhexyl methacrylate (2EHMA) were each dissolved in chloroform with MeCD. Chloroform was then evapo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0055706
更新日期：2000-01-01 00:00:00
abstract：:Ternary compatible blends of chitosan, poly(vinyl alcohol), and poly(lactic acid) were prepared by an oil-in-water (O/W) emulsion process. Solutions of chitosan in aqueous acetic acid, poly(vinyl alcohol) (PVA) in water, and poly(lactic acid) (PLA) in chloroform were blended with a high-shear mixer. PVA was used as an...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101227q
更新日期：2011-04-11 00:00:00
abstract：:The effect of solution pH during layer-by-layer assembly of polyelectrolyte multilayer (PEM) coatings on properties relevant to orthopedic implant success was investigated. Bone morphogenetic protein 2 (BMP-2), a potent osteoconductive growth factor, was adsorbed onto the surface of anodized titanium, and PEM coatings...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01730
更新日期：2016-06-13 00:00:00
abstract：:This paper shows a simple and effective way to avoid the dissociation of multimeric enzymes by coating their surface with a large cationic polymer (e.g., polyethylenimine (PEI)) by ionic exchange. As model enzymes, glutamate dehydrogenase (GDH) from Thermus thermophilus and formate dehydrogenase (FDH) from Pseudomonas...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801162e
更新日期：2009-04-13 00:00:00
abstract：:Angiogenesis has long been a desired therapeutic approach to improve clinical outcomes of conditions typified by ischemia. Vascular endothelial growth factor (VEGF) has demonstrated the ability to generate new blood vessels in vivo, but trials using intravenous delivery have not yet produced clinical success. Localize...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm061211k
更新日期：2007-05-01 00:00:00
abstract：:Thermoresponsive oligo(ethylene glycol)-based copolymers were investigated for trypsin conjugation. These copolymers have been synthesized by atom transfer radical polymerization of 2-(2-methoxyethoxy)ethyl methacrylate (MEO(2)MA) with oligo(ethylene glycol) methyl ether methacrylate (OEGMA(475), M(n) = 475 g.mol(-1))...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1005036
更新日期：2010-08-09 00:00:00
abstract：:Polystyrene-polyisocyanopeptide (PS-PIAT) polymersomes containing CALB in two different locations, one in the aqueous inner compartment and one in the bilayer, were investigated for enzymatic ring-opening polymerization of lactones in water. It is shown that the monomers 8-octanolactone and dodecalactone yield oligome...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7005938
更新日期：2007-12-01 00:00:00
abstract：:The purpose of this study was to gain mechanistic insights into the effect of different formulation parameters on the degradation and release behavior of protein-loaded nanoparticulate carrier systems based on an aliphatic polyester with pendant hydroxyl groups, poly(lactic-co-glycolic-hydroxymethyl glycolic acid) (pL...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301900t
更新日期：2013-04-08 00:00:00
abstract：:High molecular weight trimethylene carbonate (TMC) and epsilon-caprolactone (CL) (co)polymers were synthesized. Melt pressed (co)polymer films were cross-linked by gamma irradiation (25 kGy or 50 kGy) in vacuum, yielding gel fractions of up to 70%. The effects of copolymer composition and irradiation dose on the cytot...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8007988
更新日期：2008-11-01 00:00:00
abstract：:Wheat arabinoxylan was treated with two α-arabinofuranosidases exhibiting different mode of action to create three different polymeric substrates. These three substrate preparations were characterized by xylopyranose backbone sugars that are (1) singly substituted by arabinose at C2 or C3, (2) doubly substituted by ar...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00229
更新日期：2017-05-08 00:00:00
abstract：:Forisomes are protein polymers found in leguminous plants that have the remarkable ability to undergo reversible "muscle-like" contractions in the presence of divalent cations and in extreme pH environments. To gain insight into the molecular basis of forisome structure and assembly, we used confocal laser scanning mi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3008499
更新日期：2012-10-08 00:00:00
abstract：:Poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone) has been synthesized and characterized by gel permeation chromatography, Fourier transform infrared spectroscopy, NMR spectroscopy, and thermal analysis. The polymer is a chemically amplified photoresist. Arrays of lines with 25 microm width and 25 microm spacing wer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0256505
更新日期：2003-01-01 00:00:00
abstract：:Hydrophilic copolymers with high refractive index and bactericide properties based on quaternary ammonium salts monomers and methacrylates bearing benzothiazole moieties have been developed for application as foldable intraocular lenses. Composition of the systems was adjusted to get materials with optimized flexibili...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9006997
更新日期：2009-11-09 00:00:00
abstract：:This paper describes the synthesis and characterization of a novel organic polymer coating for the prevention of the growth of Pseudomonas aeruginosa on the solid surface of three-dimensional objects. Substrata were encapsulated with polyterpenol thin films prepared from terpinen-4-ol using radio frequency plasma enha...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100369n
更新日期：2010-08-09 00:00:00
abstract：:Tissue regeneration involves versatile types of cells. The accumulation and disorganized behaviors of undesired cells impair the natural healing process, leading to uncontrolled immune response, restenosis, and/or fibrosis. Cell-selective surfaces and interfaces can have specific and positive effects on desired types ...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.8b00264
更新日期：2018-06-11 00:00:00
abstract：:We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on particles were obtained by the formation of multicomposition...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400680d
更新日期：2013-08-12 00:00:00
abstract：:Electrospun poly(glycolide-co-lactide) (PLA10GA90, LA/GA ratio 10/90) biodegradable nanofiber membranes possessed very high surface area to volume ratios and were completely noncrystalline with a relatively lowered glass transition temperature. These characteristics led to very different structure, morphology, and pro...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025717o
更新日期：2003-03-01 00:00:00
abstract：:We have investigated the thermodynamic and dynamic behavior of multistranded β-lactoglobulin protein fibrils in water, by combining static, dynamic, and depolarized dynamic light scattering (SLS, DLS, DDLS), small angle neutron scattering (SANS), rheology, and cryogenic transmission electron microscopy (cryo-TEM). We ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301005w
更新日期：2012-10-08 00:00:00
abstract：:Chitin nanocrystal aqueous dispersions were prepared by acid hydrolysis of crude chitin from crab shells. The resulting dispersions were studied with small deformation oscillatory experiments and polarized optical microscopy under different conditions of nanocrystal concentration, ionic strength, pH, and temperature. ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901046c
更新日期：2010-01-11 00:00:00
abstract：:Lignin is a natural, renewable biopolymer synthesized by plants. It is a macromolecule consisting of aromatic structures with high density of functional groups making it an ideal precursor for the design of sustainable biomaterials for applications such as drug delivery. The rational design of these materials requires...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00822
更新日期：2017-08-14 00:00:00
abstract：:Nowadays, active targeting of nanotherapeutics is a challenging issue. Here, we propose a rational design of a ternary nanoassembly (SAP) composed of nonionic amphiphilic β-cyclodextrins (amphiphilic CD) incorporating pheophorbide (Pheo) as a phototherapeutic and an adamantanyl-folic acid conjugate (Ada-FA) to target ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00306
更新日期：2019-07-08 00:00:00
abstract：:Inorganic polyphosphates, linear polymers of orthophosphate, occur naturally throughout biology and have many industrial applications. Their biodegradable nature makes them attractive for a multitude of uses, and it would be important to understand how polyphosphates are turned over enzymatically. Studies of inorganic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500872g
更新日期：2014-08-11 00:00:00
abstract：:The calcium-binding sites of calbindin D(9k) have a helix-loop-helix motif. In this study, the helix motifs were replaced by several Ala-Gly repeating regions designed on the basis of the primary sequences of several silk fibroins. The synthesized peptides were treated with several organic solvents to modify the secon...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050863q
更新日期：2006-02-01 00:00:00
abstract：:Crystal conversion of beta-chitin to alpha-chitin by aq. NaOH treatment was studied for a highly crystalline beta-chitin sample from diatom spine. The minimum NaOH concentration to cause swelling was between 25% and 30% w/w. The alkali-swollen material was poorly crystalline and was regenerated as alpha-chitin on wash...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0257513
更新日期：2003-07-01 00:00:00
abstract：:Repifermin, a truncated form of fibroblast growth factor-10 (FGF-10) also known as keratinocyte growth factor-2 (KGF-2), is a heparin-binding protein with potent regenerative properties. The protein unfolds and aggregates at relatively low temperature (~37 °C). Electrostatic interactions between polyanions and several...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2007967
更新日期：2011-11-14 00:00:00
