Abstract:
:Noncontact mode atomic force microscopy was used to investigate native silk proteins prepared in different ways. Low protein concentrations revealed that single protein molecules exhibit a simple, round shape with apparent diameters of 20-25 nm. Shearing the native protein solutions after extraction from the gland and prior to drying led to a beads-on-a-string assembly at the nanometer scale. Protein concentration had a significant effect on the morphology of the protein assemblies. At higher protein concentrations, shear-induced alignment into nanofibrils was observed, while lower concentrations lead to the formation of much thinner fibrils with a width of about 8 nm.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Greving I,Cai M,Vollrath F,Schniepp HCdoi
10.1021/bm201509bsubject
Has Abstractpub_date
2012-03-12 00:00:00pages
676-82issue
3eissn
1525-7797issn
1526-4602journal_volume
13pub_type
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