Abstract:
:This paper describes the synthesis and evaluation of a series of multifunctional poly-l-glutamic acid derivatives that can be used as vectors for gene delivery. They readily form polyelectrolyte complexes with DNA, resulting in a reduced surface charge and size of the DNA. The formation of a polymer-DNA complex and the stability toward serum albumin was analyzed by ethidium bromide fluorescence measurements and agarose gel retardation studies. Most polymers, except those with more than 80% imidazoles, are able to condense calf thymus DNA, thus forming complexes with sizes varying between 105 and 172 nm. The surface charge of the complexes was determined at different charge ratios by zeta potential measurements. The buffering properties of the polymers were determined via titration studies. The results show that the polymers are able to buffer the endosomal environment, although to a smaller extent than polyethyleneimine. The first part of this study is devoted to the synthesis and the physicochemical evaluation of the multifunctional polymers and their use as carriers for genetic information. The second part, to be published subsequently, discusses the biological evaluation of the polymers and their complexes with DNA.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Dubruel P,Dekie L,Schacht Edoi
10.1021/bm034014jkeywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
1168-76issue
5eissn
1525-7797issn
1526-4602journal_volume
4pub_type
杂志文章abstract::To investigate the influence of surface-functionalized substrates with nanostructures on the behaviors of mesenchymal stem cells, we conjugated bone morphogenetic protein 2 (BMP2) onto TiO(2) nanotubes with different diameter sizes of 30, 60, and 100 nm for in vitro study. Polydopamine was employed as the intermediate...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1014365
更新日期:2011-04-11 00:00:00
abstract::Polycaprolactone (PCL) was reported a long time ago; however, its biomedical applications has not been extensively investigated in comparison with poly(lactide- co-glycolide) (PLGA) due to its too slow degradation profile. Here, we are reporting an oxalate-connected oligocaprolactone multiblock copolymer (PCL-OX) as a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00266
更新日期:2018-06-11 00:00:00
abstract::Most biopolymers exist in a plasticized state, whether it is naturally with water or unnaturally with glycerol or other suitable polyol, to make a flexible material. We have found that the extent to which a biopolymer can be plasticized is dependent on its molecular and higher order structures outside of simply molecu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800759g
更新日期:2008-11-01 00:00:00
abstract::Spiders have evolved a complex system of silk producing glands. Each of the glands produces silk with strength and elasticity tailored to its biological purpose. Sequence analysis of the major ampullate silk reveals four highly conserved concatenated blocks of amino acids: (GA) n , A n , GPGXX, and GGX. While the GPGX...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701124p
更新日期:2008-06-01 00:00:00
abstract::A multiple targeted drug carrying bilayer membrane for preventing an abdominal adhesion is prepared by electrospinning. Two bioactive drugs were successfully incorporated into this bilayer membrane and can be independently released from nanofibrous scaffolds without losing structural integrity and functionality of the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301997e
更新日期:2013-04-08 00:00:00
abstract::Nowadays the open and the patent literatures propose a large number of polymers whose main chains can be degraded usefully. Among these degradable polymers, aliphatic polyester-based polymeric structures are receiving special attention because they are all more or less sensitive to hydrolytic degradation, a feature of...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm0494702
更新日期:2005-03-01 00:00:00
abstract::For developing broader application of biobased polymers, graft copolymers and comb polymers having poly(lactic acid) (PLA) side chains have been synthesized by using a macromonomer technique. PLA macromonomers (MMm) having a methacryloyl polymerizable group with different PLA chain length with an average length m = 4,...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301212a
更新日期:2012-11-12 00:00:00
abstract::The need for polymers for high-end applications, coupled with the desire to mimic nature's macromolecular machinery fuels the development of innovative synthetic strategies every year. The recently acquired macromolecular-synthetic tools increase the precision and enable the synthesis of polymers with high control and...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/acs.biomac.5b01698
更新日期:2016-03-14 00:00:00
abstract::Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore electrospinning for protein-based bi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025581u
更新日期:2002-11-01 00:00:00
abstract::Synthesis of novel zwitterionic block copolypeptides, poly(N-isopropylacrylamide)-block-poly(L-glutamic acid-co-L-lysine) [PNiPAM(n)(PLG(x)-co-PLLys(y))m , where n is the number-average degree of polymerization (DP(n)) of PNiPAM block, x and y are the mole fraction of glutamic acid and lysine residues, respectively, a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800394p
更新日期:2008-10-01 00:00:00
abstract::We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO(2)), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900589r
更新日期:2009-10-12 00:00:00
abstract::This study shows the application of the electrospinning technique as a viable method for the encapsulation and stabilization of bifidobacterial strains. Poly(vinyl alcohol) (PVOH) was used as the encapsulating material because it is generally recognized as safe (GRAS), has a high oxygen barrier when dry, and is water ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900660b
更新日期:2009-10-12 00:00:00
abstract::Enzymatic adsorption and degradation of three types of aliphatic polyester films and two types of polyolefin films by the extracellular PHB depolymerase from Alcaligenes faecalis T1 have been studied by using a quartz crystal microbalance (QCM) technique. Hydrolysis of poly[(R)-3-hydroxybutyrate] was quantitatively fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0000844
更新日期:2001-04-01 00:00:00
abstract::The resistance developed by life-threatening bacteria toward conventional antibiotics has become a major concern in public health. To combat antibiotic resistance, there has been a significant interest in the development of antimicrobial cationic polymers due to the ease of synthesis and low manufacturing cost compare...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01385
更新日期:2017-01-09 00:00:00
abstract::Bioresponsive polymers can enable the development of more effective drug delivery vehicles and medical materials. Acetal-modified polysaccharides allow pH-triggered solubility switching in a versatile and effective manner, but prior work has been limited to a combination of acyclic alkoxyisopropyl and cyclic isopropyl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00215
更新日期:2019-05-13 00:00:00
abstract::Recent research indicates that the progression of Parkinson's disease can start from neurons of the enteric nervous system, which are in close contact with the gastrointestinal epithelium: α-synuclein molecules can be transferred from these epithelial cells in a prion-like fashion to enteric neurons. Thin mucus layers...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00905
更新日期:2019-12-09 00:00:00
abstract::The Bacillus subtilis protease Proleather FG-F catalyzed the transesterification of inulin with vinyl acrylate (VA) in dimethylformamide (DMF). The reaction conversion for different VA concentrations was greater than 57% after 96 h at 50 degrees C. The degree of substitution (DS, defined as the amount of acrylate grou...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm010150h
更新日期:2002-03-01 00:00:00
abstract::Cellulose nanocrystals (CNCs) are mechanically rigid, toxicologically benign, fiber-like nanoparticles. They can easily be extracted from renewable biosources and have attracted significant interest as reinforcing fillers in polymers. We here report the modification of CNCs with the 2-ureido-4[1H]pyrimidinone (UPy) mo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01639
更新日期:2017-02-13 00:00:00
abstract::The viscous mucus coating that adheres to the epithelial surfaces of mammalian organs provides protection for the underlying tissues and is an efficient barrier to drug delivery. Pulsed-gradient spin-echo NMR and small-angle neutron scattering have been used to study the aqueous solution interaction of various model p...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9009667
更新日期:2010-01-11 00:00:00
abstract::Hydrogels derived from resilin-like polypeptides (RLPs) have shown outstanding mechanical resilience and cytocompatibility; expanding the versatility of RLP-based materials via conjugation with other polypeptides and polymers would offer great promise in the design of a range of materials. Here, we present an investig...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01255
更新日期:2016-01-11 00:00:00
abstract::The maintenance of hepatocyte phenotype and functions remains as a great challenge in the generation of functional liver tissue and in vitro model for drug metabolism studies. The use of hepatocyte coculture systems plays essential roles in the establishment of cell-cell and cell-extracellular matrix communications si...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401926k
更新日期:2014-03-10 00:00:00
abstract::We present a new design of peptide-polymer conjugates where a polymer chain is covalently linked to the side chain of a helix bundle-forming peptide. The effect of conjugated polymer chains on the peptide structure was examined using a de novo designed three-helix bundle and a photoactive four-helix bundle. Upon attac...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800113g
更新日期:2008-08-01 00:00:00
abstract::Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modificati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00470
更新日期:2015-07-13 00:00:00
abstract::A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based on three different core sizes (18, 35, 65 nm) and five polymer...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300248u
更新日期:2012-05-14 00:00:00
abstract::Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049941k
更新日期:2004-07-01 00:00:00
abstract::Novel biodegradable materials with tunable hydrolytic degradation rate are prepared by grafting of phosphonoethylated polyglycidols with polyesters. First, the hydrolytically degradable polyester grafts are attached to polyglycidols partially grafted with phosphonoethylated diethyl esters through chemical-catalyzed gr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401428b
更新日期:2013-11-11 00:00:00
abstract::There is compelling evidence that, beyond their traditional role in hemostasis and thrombosis, platelets play a significant role in mediating hematologic mechanisms of tumor metastasis by directly and indirectly interacting with pro-metastatic cancer cells. With this rationale, we hypothesized that platelets can be an...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301996p
更新日期:2013-03-11 00:00:00
abstract::Herein, we developed a new gene delivery vector by grafting a betaine monomer (N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate, DMAAPS) onto 25 KDa polyethylenimine (PEI 25K) via the Michael addition reaction. The graft ratio for betaine on PEI polymer could be readily controlled, and in this study three PEI-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301826m
更新日期:2013-03-11 00:00:00
abstract::Weissella confusa VTT E-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked D-glucosyl units and very few α-(1→3) branch linkages. A mixture of the Chaetomium erraticum endodextranase and the Aspergillus niger α-glucosidase was used to hydrolyze W. confusa dextran to glucose and a set...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1011536
更新日期:2011-02-14 00:00:00
abstract::A novel cellulose derivative, 6-O-dihydrophytylcellulose (DHPC), was first synthesized via a ring-opening polymerization and allowed to self-assemble onto an air-water interface. Langmuir-Blodgett (LB) films were characterized with atomic force microscope (AFM), UV-vis spectroscopy, and Fourier transform infrared spec...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060083x
更新日期:2006-06-01 00:00:00