Abstract:
:The encapsulation of live cells with polymeric coat-ings is a versatile approach to modulate or control the response cells to their environment. The layer-by-layer (LbL) self-assembly of nonimmunogenic polyelectrolytes is employed here to attenuate or suppress the binding of antibodies to live red blood cells (RBCs) and, consequently, decrease their inherent immunogenicity toward foreign RBCs. The optimized shell was composed of four bilayers of alginate (AL) and chitosan-graft-phosphorylcholine (CH-PC) surrounded by two bilayers of AL and poly-l-lysine-graft-polyethylene glycol (PLL-PEG). Experimental parameters, including the polyelectrolytes and RBCs concentrations and the cell handling and purification protocols, were optimized to achieve effective encapsulation of live and functional RBCs in suspension. The viability and functionality of coated RBCs were confirmed by a hemolysis assay and by their ability to take up oxygen. The successful immunocamouflage of RBCs was confirmed by observing that the recognition of the ABO/D (Rh) blood group antigens present on the surface of RBCs by their respective antibodies was muted in the case of coated RBCs. The results of this studies mark an important step toward the production of universal RBCs.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Mansouri S,Merhi Y,Winnik FM,Tabrizian Mdoi
10.1021/bm101200csubject
Has Abstractpub_date
2011-03-14 00:00:00pages
585-92issue
3eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract::A variety of important biological events are mediated by the multivalent interaction between relevant oligosaccharides and multiple saccharide receptors on lectins, toxins, and cell surfaces; a variety of glycopolymeric materials have therefore been investigated in studies aimed at manipulating these events. The synth...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050672n
更新日期:2006-02-01 00:00:00
abstract::Hydrogels have been widely explored for biomedical applications, with injectable hydrogels being of particular interest for their ability to precisely deliver drugs and cells to targets. Although these hydrogels have demonstrated satisfactory properties in many cases, challenges still remain for commercialization. In ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01148
更新日期:2016-11-14 00:00:00
abstract::Natural silk spinning offers several advantages over the synthetic fiber spinning, although the underlying mechanisms of this process are yet to be fully elucidated. Silkworm silks, specifically B. mori, comprise two main proteins: fibroin, which forms the fiber, and sericin, a coextruded coating that acts as a matrix...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00549
更新日期:2017-08-14 00:00:00
abstract::In this paper we extend our previous experimental work on interaction between polyelectrolytes and liposomes. First, the adsorption of chitosan and alkylated chitosan (cationic polyelectrolytes) with different alkyl chain lengths on lipid membranes of liposomes is examined. The amount of both chitosans adsorbed remain...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800400y
更新日期:2008-08-01 00:00:00
abstract::A systematic study is carried out to understand how physical and covalent crosslinks affect the mechanical properties of an eight-arm poly(ethyleneglycol)-based hydrogel. Heparin and heparin-binding peptide are used as a physical crosslinker, and an enzymatically cleavable peptide with a cysteine on each end serves as...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801270k
更新日期:2009-05-11 00:00:00
abstract::The stability of internally cross-linked casein micelles against disruption by urea (which disrupts hydrogen bonds and hydrophobic interactions) and trisodium citrate (which sequesters micellar calcium phosphate) was investigated. Addition of urea (0-6 mol L-1) and/or citrate (0-50 mmol L-1) progressively reduced the ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm061070m
更新日期:2007-04-01 00:00:00
abstract::Hierarchical nanocomposite films with Pd-PdO nanoparticles anchored uniformly on the inner surface of TiO2 nanotubes were achieved through a stepwise bioredox/artificial oxygenation approach by using the natural eggshell membrane (ESM) as a template. The Pd content ratio of Pd-PdO loading could be arbitrarily varied f...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700993m
更新日期:2008-02-01 00:00:00
abstract::The molecular recognition features of a DNA-sensitive fluorescent bioconjugate capable of targeting a specific DNA sequence with high efficiency are described. The bioconjugate combines a polypeptide from the Tc3 transposase DNA-binding domain with the dsDNA-sensitive fluorophore thiazole orange. Fluorescence spectros...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070334h
更新日期:2007-11-01 00:00:00
abstract::A modular approach to engineering cross-linked elastic biomaterials is presented for fine-tuning of material mechanical and biological properties. The three components, soluble elastin, hyaluronic acid, and silk fibroin, contribute with different features to the overall properties of the final material system. The ela...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1007772
更新日期:2010-09-13 00:00:00
abstract::The rational design of a polyplex gene carrier aims to balance maximal effectiveness of nucleic acid transfection into cells with minimal adverse effects. Depsipeptide blocks with an Mn ∼ 5 kDa exhibiting strong physical interactions were conjugated with PEI moieties (2.5 or 10 kDa) to di- and triblock copolymers. Upo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01034
更新日期:2017-11-13 00:00:00
abstract::meso-Tetra(N-methyl-4-pyridyl) porphine tetra tosylate (TMP) is a photosensitizer that can be used in photodynamic therapy (PDT) to induce cell death through generation of reactive oxygen species in targeted tumor cells. However, TMP is highly hydrophilic, and therefore, its ability to accumulate intracellularly is li...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301858a
更新日期:2013-02-11 00:00:00
abstract::The adhesion of wet regenerated cellulose to colloidal complexes formed between carboxymethyl cellulose (CMC) and polyvinylamine (PVAm) was evaluated by measuring the force to delaminate pairs of regenerated cellulose membranes bound together with polyelectrolyte complex. The most important parameter was the surface c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070307r
更新日期:2007-07-01 00:00:00
abstract::Coniferyl alcohol was polymerized in pectin solution in order to mimic the lignification that is the final step of biosynthesis of plant cell wall. Dehydrogenated polymers (DHP = coniferyl alcohol polymers = synthetic lignin) interact with pectin to form hydrophobic clusters as monitored by pyrene fluorescence spectro...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049390y
更新日期:2005-03-01 00:00:00
abstract::Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301110s
更新日期:2012-12-10 00:00:00
abstract::Molecular orientation distributions in the crystalline and amorphous regions of a biaxially oriented poly(L-lactic acid) film were analyzed fully by polarized Raman spectroscopy. Raman bands at 926 and 875 cm(-1) were chosen for the determination of the most probable molecular orientation distribution functions for th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060326l
更新日期:2006-09-01 00:00:00
abstract::Agmatine-containing bioreducible polymer, poly(cystaminebis(acrylamide)-agmatine) (poly(CBA-AG)) was synthesized for gene delivery systems. It could form 200-300 nm sized and positively charged polyplexes with pDNA, which could release pDNA in reducing the environment due to the internal disulfide bonds cleavage. Poly...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00590
更新日期:2015-09-14 00:00:00
abstract::We have designed, synthesized, and characterized peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. 5-Azid...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200897b
更新日期:2011-10-10 00:00:00
abstract::Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft pulp with amino groups (F) and further immobilization of a cross-linked glucose oxidase (G*) from Aspergillus niger. The cross-linked enzymes (G*) were characterized using X-ray spectroscopy, Fourier transform infrared spectroscop...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00865
更新日期:2016-10-10 00:00:00
abstract::Silk fibroin (SF) protein, produced by silkworm Bombyx mori, is a promising biomaterial, while sophorolipid (SL) is an amphiphilic functional biosurfactant synthesized by nonpathogenic yeast Candida bombicola. SL is a mixture of two forms, acidic (ASL) and lactonic (LSL), which when added to SF results in accelerated ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01069
更新日期:2016-10-10 00:00:00
abstract::Properties of Aloe vera galacturonate hydrogels formed via Ca(2+) crosslinking have been studied in regard to key parameters influencing gel formation including molecular weight, ionic strength, and molar ratio of Ca(2+) to COO(-) functionality. Dynamic oscillatory rheology and pulsed field gradient NMR (PFG-NMR) stud...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8008457
更新日期:2008-11-01 00:00:00
abstract::Chitin is a nitrogen-rich polysaccharide that is abundant in crustaceans, mollusks, insects, and fungi and is the second most abundant organic material found in nature next to cellulose. Chitosan, the N-deacetylated derivative of chitin, is environmentally friendly, nontoxic, biodegradable, and antibacterial. Fibrous ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7006983
更新日期:2007-09-01 00:00:00
abstract::C13-dipeptides that did not gel on their own were found to form hydrogels when combined with mixtures (coassembly). At pH = 4.6, by mixing negatively charged C13-WD (C13-WD2- and/or C13-WD-) with C13-KW or C13-YK, where the side chain of K carried positive charge, two composite hydrogels with different mechanical prop...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01325
更新日期:2020-12-14 00:00:00
abstract::The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. The dynamics and extent of related adsorption processes were monitored by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups were instal...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201236a
更新日期:2011-12-12 00:00:00
abstract::The long-term stability and success of orthopedic implants depend on the osseointegration process, which is strongly influenced by the biomaterial surface. A promising approach to enhance implant integration involves the modification of the surface of the implant by means of polymers that mimic the natural components ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3002683
更新日期:2012-05-14 00:00:00
abstract::An underwater bioadhesive generally comprises a multiprotein complex that provides a molecular basis for self-assembly. We report here a new class of self-assembling peptide inspired by a 20 kDa barnacle cement protein. Studies on the chemically synthesized 24-residue peptide have revealed that (1) it underwent irreve...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0612236
更新日期:2007-06-01 00:00:00
abstract::Mixed protein-based hydrogels have been prepared by blending gelatin (G) with amorphous Bombyx mori silk fibroin (SF) and promoting beta-crystallization of SF via subsequent exposure to methanol or methanol/water solutions. The introduction of beta crystals in SF serves to stabilize the hydrogel network and extend the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050396c
更新日期:2005-11-01 00:00:00
abstract::Glycerol derivatives of cutin and suberin monomers were synthesized by acid catalysis. Their dispersion in an aqueous solution was examined by phase contrast microscopy, neutron scattering, and solid state NMR. It is shown that the phase behavior strongly depends on the nature of the derivatives forming either lumps o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049325o
更新日期:2005-01-01 00:00:00
abstract::Self-assembling amphiphilic designer peptides have been successfully applied as nanomaterials in biomedical applications. Understanding molecular interactions at the peptide-membrane interface is crucial, since interactions at this site often determine (in)compatibility. The present study aims to elucidate how model m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01089
更新日期:2016-11-14 00:00:00
abstract::Macromolecule-based therapeutic agents, particularly proteins, antigens, monoclonal antibodies, transcription factors, nucleic acids, and gene editing enzymes, have the potential to offer cures for previously untreatable diseases. However, they present an enormous delivery challenge due to poor absorption and rapid me...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01754
更新日期:2020-04-13 00:00:00
abstract::Silkworm silk has excellent mechanical properties, biocompatibility, and promising applications in the biomedical sector. Silkworms spin silk at the beginning and end of each of their five instar stages, as well as spinning mature silk after the fifth instar. We evaluated the mechanical properties and structure of 10 ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01576
更新日期:2019-03-11 00:00:00