Abstract：
:The development and use of synthetic, cross-linked, macromolecular substrates with tunable elasticity has been instrumental in revealing the mechanisms by which cells sense and respond to their mechanical microenvironment. We here describe a hydrogel based on radical-free, cross-linked poly(ethylene glycol) to study the effects of both substrate elasticity and type of adhesive coating on fibroblast adhesion and migration. Hydrogel elasticity was controlled through the structure and concentration of branched precursors, which efficiently react via Michael-type addition to produce the polymer network. We found that cell spreading and focal adhesion characteristics are dependent on elasticity for all types of coatings (RGD peptide, fibronectin, vitronectin), albeit with significant differences in magnitude. Importantly, fibroblasts migrated slower but more persistently on stiffer hydrogels, with the effects being more pronounced on fibronectin-coated substrates. Therefore, our results validate the hydrogels presented in this study as suitable for future mechanosensing studies and indicate that cell adhesion, polarity, and associated migration persistence are tuned by substrate elasticity and biochemical properties.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Missirlis D,Spatz JPdoi
10.1021/bm4014827subject
Has Abstractpub_date
2014-01-13 00:00:00pages
195-205issue
1eissn
1525-7797issn
1526-4602journal_volume
15pub_type
杂志文章abstract：:We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200780r
更新日期：2011-10-10 00:00:00
abstract：:Cellulose is the most familiar and most abundant strong biopolymer, but the reasons for its outstanding mechanical performance are not well understood. Each glucose unit in a cellulose chain is joined to the next by a covalent C-O-C linkage flanked by two hydrogen bonds. This geometry suggests some form of cooperativi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401616n
更新日期：2014-03-10 00:00:00
abstract：:Oligonucleotide-spider silk conjugates can be placed on silicon wafers by complementary DNA strands, which are coupled chemically to the surface. Such specific immobilization of spider silk proteins allows the nucleation and guided growth of β-sheet-rich nanofibrils in the presence of phosphate ions on the surface. Ad...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01333
更新日期：2019-01-14 00:00:00
abstract：:A new α-amylase from Rhizomucor sp. (RA) was studied in detail due to its very efficient hydrolysis of raw starch granules at low temperature (32 °C). RA contains a starch binding domain (SBD) connected to the core amylase catalytic domain by a O-glycosylated linker. The mode of degradation of native maize starch gran...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100913z
更新日期：2011-01-10 00:00:00
abstract：:Inflammation plays an important role in symptomatic intervertebral disc degeneration and is associated with the production of neurotrophins in sensitizing innervation into the disc. The use of high molecular weight (HMw) hyaluronic acid (HA) hydrogels offers a potential therapeutic biomaterial for nucleus pulposus (NP...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00168
更新日期：2015-06-08 00:00:00
abstract：:Heparins are widely used to prevent blood clotting during surgeries and for the treatment of thrombosis. However, bleeding associated with heparin therapy is a concern. Protamine, the only approved antidote for unfractionated heparin (UFH) could cause adverse cardiovascular events. Here, we describe a unique molecular...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00994
更新日期：2017-10-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400680d
更新日期：2013-08-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01103
更新日期：2020-10-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200899h
更新日期：2011-10-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900149b
更新日期：2009-06-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400846h
更新日期：2013-10-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00244
更新日期：2020-08-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300497c
更新日期：2012-07-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00306
更新日期：2019-07-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3003286
更新日期：2012-06-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00413
更新日期：2015-06-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200413a
更新日期：2011-06-13 00:00:00
abstract：:Laminin is a heterotrimeric glycoprotein with a key role in the formation and maintenance of the basement membrane architecture and properties, as well as on the modulation of several biological functions, including cell adhesion, migration, differentiation and matrix-mediated signaling. In the central nervous system ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01319
更新日期：2020-02-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00932
更新日期：2016-09-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049383+
更新日期：2005-01-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm058003x
更新日期：2005-07-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00227
更新日期：2015-04-13 00:00:00
abstract：:To develop a safe, effective, and biocompatible gene delivery vector, a series of hydrophobic amino acid grafted chitosan (AGC) derivatives were synthesized by carbodiimide mediated coupling reaction. Chemical characterization of AGC polymers was performed by NMR and elemental analysis. AGC polymers demonstrated excel...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301720g
更新日期：2013-02-11 00:00:00
abstract：:Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900889b
更新日期：2010-01-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050597g
更新日期：2006-04-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200897b
更新日期：2011-10-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00180
更新日期：2015-04-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501394m
更新日期：2015-01-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2004912
更新日期：2011-10-10 00:00:00
abstract：:Limited studies have been done on the interactions between subunits of self-assembling protein cages. E2 protein cage from Bacillus stearothermophilus was investigated in this work to impart pH-sensitive disassembly profile. Key amino acids were identified at the intratrimer and intertrimer interfaces, and histidine r...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2005438
更新日期：2011-09-12 00:00:00
