Abstract:
:In tissue engineering, incorporation of bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable bone formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(ε-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better bone formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential bone formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved bone regeneration.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Zhang Q,Tan K,Zhang Y,Ye Z,Tan WS,Lang Mdoi
10.1021/bm401309usubject
Has Abstractpub_date
2014-01-13 00:00:00pages
84-94issue
1eissn
1525-7797issn
1526-4602journal_volume
15pub_type
杂志文章abstract::Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or kappa-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by ce...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8002754
更新日期:2008-08-01 00:00:00
abstract::In the present study, novel bionanocomposite materials with tunable properties were successfully prepared using a poly(lactic acid) (PLA) matrix and acetylated microfibrillated cellulose (MFC) as reinforcing agent. The acetylation of MFC was confirmed by FTIR and (13)C CP-MAS NMR spectroscopies. The grafting of acetyl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901186u
更新日期:2010-02-08 00:00:00
abstract::Adherence and survival of pathogenic bacteria on surfaces leading to concomitant transmission to new hosts significantly contributes to the proliferation of pathogens, which in turn considerably increases the threat to human health, particularly by antibiotic-resistant bacteria. Consequently, more research into effect...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200718s
更新日期:2011-10-10 00:00:00
abstract::The antiproliferative activity of alkylated heparin, in which the terminal end of heparin is derivatized with an alkyl group (butyl, octyl, lauryl, stearyl), was examined using vascular smooth muscle cells. The proliferation of cells, which were growth-arrested prior to addition of heparin, was inhibited in proportion...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm010097x
更新日期:2001-01-01 00:00:00
abstract::We have designed, synthesized, and characterized peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. 5-Azid...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200897b
更新日期:2011-10-10 00:00:00
abstract::There is a lack of hydrogel materials whose properties can be tuned at the point of use. Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds because of their low mechanical strength. Their mechanics can be improved through cross-linking and chem...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01251
更新日期:2016-03-14 00:00:00
abstract::The conformation of a single elastin-like peptide GVG(VPGVG)3 in liquid water is studied by computer simulations in the temperature interval between 280 and 440 K. Two main conformational states of the peptide can be distinguished: a rigid conformational state, dominating at low temperatures, and a flexible conformati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070233j
更新日期:2007-07-01 00:00:00
abstract::Silk fibroin hydrogels with tunable properties could be obtained from aqueous fibroin solutions (4.2 w/v %) in a short period of time. This was achieved by the addition of ethylene glycol diglycidyl ether (EGDE) into the reaction solution. Introduction of EGDE cross-links between the fibroin molecules decreases the mo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300006r
更新日期:2012-04-09 00:00:00
abstract::Polypeptide-based formulations that undergo liquid to hydrogel transitions upon change in temperature have become desirable targets since they can be mixed with cells or injected into tissues as liquids, and subsequently transform into rigid scaffolds or depots. Such materials have been challenging to prepare using sy...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00124
更新日期:2015-04-13 00:00:00
abstract::The effect of moisture content and temperature on water diffusion into a modified high amylose (< or = 90%) maize thermoplastic starch blend was investigated. Gravimetric and magnetic resonance imaging (MRI) studies were conducted to elucidate the diffusion mechanism and diffusion coefficients for this system. The dif...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060791i
更新日期:2007-01-01 00:00:00
abstract::Poly((N-vinylpyrrolidone)-block-poly(vinyl acetate)) (PVP-b-PVAc) block copolymers of varying molecular weight and hydrophobic block lengths were synthesized via controlled radical polymerization and investigated as carriers for the solubilization of highly hydrophobic riminophenazine compounds. These compounds have r...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301410d
更新日期:2012-12-10 00:00:00
abstract::A new series of cationic, thermo-sensitive, and biodegradable poly(L-lactide)-poly(2-ethyl-2-oxazoline)-poly(L-lactide) (PLLA-PEOz-PLLA) triblock copolymers were synthesized by ring-opening polymerization. With increasing molecular weight and crystallinity of hydrophobic PLLA blocks, the critical micellization concent...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034190s
更新日期:2003-11-01 00:00:00
abstract::The order of assembly of a magnetic nanoparticle (MNP) vector comprised of the same components (MNP, PEI, and plasmid DNA) on entry mechanism, intracellular localization, and viability of BHK21 cells was investigated. Cellular uptake measurements under four different uptake inhibiting conditions, such as low temperatu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100748p
更新日期:2010-09-13 00:00:00
abstract::The conformational behavior of a long single-chain double-stranded DNA in solutions of free silver ions and silver nanoparticles generated via the reduction of AgNO3 by NaBH4 was monitored by fluorescence and electron microscopies and UV spectroscopy. The interaction of monovalent silver ions with DNA induces shrinkin...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800235j
更新日期:2008-07-01 00:00:00
abstract::Biobased poly(γ-butyrolactone) (PγBL) as a fully biodegradable and bioabsorbable biomaterial has shown superior properties compared to those of other aliphatic polyesters. It is of great importance to prepare amphiphilic block copolymer containing PγBL block to make ordered nano-objects for biomedical applications suc...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01239
更新日期:2019-01-14 00:00:00
abstract::We report the preparation, characterization, and mechanical properties of DNA/poly(allylamine hydrochloride) (PAH) multilayer microcapsules. The DNA/PAH multilayers were first constructed on a planar support to examine their layer-by-layer buildup. Surface plasmon resonance spectroscopy (SPR) showed a nonlinear growth...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049254t
更新日期:2005-05-01 00:00:00
abstract::A strategy is presented that exploits the ability of synthetic polymers of different nature to disturb the strong self-assembly capabilities of amyloid based β-sheet forming peptides. Following a convergent approach, the peptides of interest were synthesized via solid-phase peptide synthesis (SPPS) and the polymers vi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3007117
更新日期:2012-09-10 00:00:00
abstract::Bone fractures constitute a major cause of morbidity and mortality especially in the elderly. Complications associated with osteoporosis drugs and the age of the patient slow bone turnover and render such fractures difficult to heal. Increasing the speed of fracture repair by administration of a fracture-targeted bone...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00777
更新日期:2015-10-12 00:00:00
abstract::Inorganic polyphosphate (polyP) released by human platelets has recently been shown to activate blood clotting and identified as a potential target for the development of novel antithrombotics. Recent studies have shown that polymers with cationic binding groups (CBGs) inhibit polyP and attenuate thrombosis. However, ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00327
更新日期:2018-04-09 00:00:00
abstract::We have constructed a tandem gene expression cassette containing three Ralstonia eutropha poly[(R)-3-hydroxybutyrate] (PHB) synthesis genes under the control of the Pichia pastoris glyceraldehyde-3-phosphate promoter and the green fluorescent protein (Gfp) under the control of the P. pastoris alcohol oxidase promoter....
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0494141
更新日期:2005-03-01 00:00:00
abstract::Thin films of a biocompatible and nonbiofouling poly(oligo(ethylene glycol) methacrylate) ( pOEGMA) with various thicknesses were formed on gold and Si/SiO 2 substrates by a combination of the formation of self-assembled monolayers (SAMs) terminating in bromoester-an initiator of atom transfer radical polymerization (...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7009043
更新日期:2007-12-01 00:00:00
abstract::Spherulites of poly(L-lactic acid) (PLLA) and of its blends with atactic poly(3-hydroxybutyrate) (a-PHB, from 10 to 75 wt %) were investigated by microfocus X-ray diffraction using synchrotron radiation. Radial scans in 5 microm steps with 3 microm beam diameter were performed. In PLLA, tens of identical diffraction i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0343951
更新日期:2004-03-01 00:00:00
abstract::G-quadruplex is a type of DNA secondary structure formed by specific guanine-rich sequences. Because of their enrichment in functional genomic regions and their biological significance, G-quadruplexes are recognized as significant drug targets for cancer and other diseases. Here, we tested the precipitation efficiency...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100862k
更新日期:2010-12-13 00:00:00
abstract::Chemo-photodynamic combined therapy is promising in cancer treatment, although low tissue penetration of visible light for activating photosensitizers (e.g., chlorin e6, Ce6) limited its broad applications. Combination of upcoverting nanoparticles (UCNPs) with the photosensitizers endows us with the possibility to uti...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01211
更新日期:2019-10-14 00:00:00
abstract::Injectable in situ crosslinkable biomaterials seeded with multipotent progenitor cells and coupled with minimally invasive arthroscopic techniques are an attractive alternative for treating irregularly shaped osteochondral defects. An in situ crosslinkable poly(lactide-co-ethylene oxide-co-fumarate) (PLEOF) macromer h...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060671a
更新日期:2007-03-01 00:00:00
abstract::The average pore size of hydrated dextran microspheres is derived from rheological and protein release data. The microspheres were prepared by cross-linking an aqueous solution of methacrylated dextran emulsified in a continuous poly(ethylene glycol) phase. The rheological data were obtained using a novel micromanipul...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005574a
更新日期:2000-01-01 00:00:00
abstract::Preparations of the highly ordered monoantennary, homofunctional diantennary, and heterofunctional diantennary neoglycopolymers of α-d-mannose and β-d-glucose residues were achieved via ring-opening metathesis polymerization. Isothermal titration calorimetry measurements of these synthetic neoglycopolymers with Concan...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01380
更新日期:2015-12-14 00:00:00
abstract::(1,3)(1,4)-β-D-Glucan (mixed-linkage glucan or MLG), a characteristic hemicellulose in primary cell walls of grasses, was investigated to determine both its role in cell walls and its interaction with cellulose and other cell wall polysaccharides in vitro. Binding isotherms showed that MLG adsorption onto microcrystal...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5001247
更新日期:2014-05-12 00:00:00
abstract::Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. He...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00823
更新日期:2015-09-14 00:00:00
abstract::The interaction of polysaccharides with water has a critical impact on their biological function as well as their technological applications. We performed ellipsometry experiments at different relative humidities (RH) to measure the equilibrium swelling of ultrathin films of different polysaccharides: native and modif...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01098
更新日期:2020-12-14 00:00:00