Abstract：
:Selectively spooling single plasmid DNA (pDNA), as a giant polyelectrolyte, into a nanosized toroidal structure or folding it into a rod-like structure has been accomplished by polyion complexation with block catiomers to form polymeric micelles in varying NaCl concentrations. The interactive potency between the pDNA and block catiomers was determined to play a critical role in defining the ultimate structure of the pDNA; the formation of toroidal or rod-like structures was achieved by complexation in 600 or 0 mM NaCl solutions, respectively. Compared with the rod-like structure, the toroidal structure possessed superior biological functions capable not only of elevating in vitro transcription but also of elevating in vivo gene transduction efficiency. This demonstrated the great utility of the toroidal pDNA packaging as a distinct structured gene carrier. Furthermore, the fact that the NaCl concentration at which the toroidal structure was specifically formed corresponds to seawater stimulates interest in this ordered nanostructure as a possible inherent structure for DNA.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Li Y,Osada K,Chen Q,Tockary TA,Dirisala A,Takeda KM,Uchida S,Nagata K,Itaka K,Kataoka Kdoi
10.1021/acs.biomac.5b00491subject
Has Abstractpub_date
2015-09-14 00:00:00pages
2664-71issue
9eissn
1525-7797issn
1526-4602journal_volume
16pub_type
杂志文章abstract：:Neutron reflectometry was used to investigate effects of calcium ions on the interfacial behavior of beta-casein at the silicon oxide-aqueous solution interface. The structural characteristics of the adsorbed layer were determined from reflectivity curves fitted to three- and two-layer optical models. The results show...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034301n
更新日期：2004-03-01 00:00:00
abstract：:Hydrogels are promising materials for biomedical applications such as tissue engineering and controlled drug release. In the past two decades, the peptide hydrogel subclass has attracted an increasing level of interest from the scientific community because of its numerous advantages, such as biocompatibility, biodegra...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01840
更新日期：2017-03-13 00:00:00
abstract：:The fate of intravenously injected nanoparticles (NPs) is significantly affected by nano-protein interaction and corona formation. However, such an interaction between NPs and digestive enzymes occurring in the gastrointestinal tract (GIT) and its impacts on epithelial cell uptake are little known. We synthesized the ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00175
更新日期：2019-04-08 00:00:00
abstract：:Regenerated silk fibroin (RSF) fibers were obtained by extruding a concentrated aqueous silk fibroin solution into an ammonium sulfate coagulation bath. A custom-made simplified industrial-type wet-spinning device with continuous mechanical postdraw was used. The effect of dope concentration, coagulation bath, extrusi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900840h
更新日期：2010-01-11 00:00:00
abstract：:We report the preparation, characterization, and mechanical properties of DNA/poly(allylamine hydrochloride) (PAH) multilayer microcapsules. The DNA/PAH multilayers were first constructed on a planar support to examine their layer-by-layer buildup. Surface plasmon resonance spectroscopy (SPR) showed a nonlinear growth...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049254t
更新日期：2005-05-01 00:00:00
abstract：:In the present study, novel bionanocomposite materials with tunable properties were successfully prepared using a poly(lactic acid) (PLA) matrix and acetylated microfibrillated cellulose (MFC) as reinforcing agent. The acetylation of MFC was confirmed by FTIR and (13)C CP-MAS NMR spectroscopies. The grafting of acetyl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901186u
更新日期：2010-02-08 00:00:00
abstract：:In situ cross-linkable hybrid hydrogels composed of gelatin and 4-arm-polypropylene oxide-polyethylene oxide (Tetronic) was developed as an injectable scaffold for tissue regeneration. The gelatin was modified by hydroxyphenyl propionic acid (HPA) and the Tetronic was conjugated with tyramines (Tet-TA). The hydrogels ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201712z
更新日期：2012-03-12 00:00:00
abstract：:Sequential copolymerizations of trimethylene carbonate (TMC) and l-lactide (LLA) were performed with 2,2-dibutyl-2-stanna-1,3-oxepane as a bifunctional cyclic initiator. The block lengths were varied via the monomer/initiator and via the TMC/l-lactide ratio. The cyclic triblock copolymers were transformed in situ into...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049393a
更新日期：2005-01-01 00:00:00
abstract：:Benefiting from their inherent localized and controlled release properties, hydrogels are ideal delivery systems for therapeutic drugs or nanoparticles. In particular, applications of hydrogels for the delivery and release of photoresponsive drugs or nanoparticles are receiving increasing attention. However, the effec...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00725
更新日期：2017-10-09 00:00:00
abstract：:We investigated the rheological properties and the penetration of differently sized carboxylated nanoparticles in pig pulmonary mucus, on different distance and time scales. Nanoparticles were either mechanically mixed into the mucus samples or deposited as an aerosol, the latter resembling a more physiologically rele...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00164
更新日期：2016-04-11 00:00:00
abstract：:The main objective of the present work was the development of new nanoparticulate carrier systems for the delivery of plasmid DNA. These new carriers consist of a blend matrix formed by a poly(lactic-co-glycolic acid) (PLGA) copolymer and polyoxyethylene derivatives. More specifically, we have prepared nanostructures ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049577p
更新日期：2005-01-01 00:00:00
abstract：:In this work we describe the application of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers as polymeric surfactants in miniemulsion techniques. HPMA-based copolymers with different ratios of HPMA (hydrophilic) to laurylmethacrylate (LMA; hydrophobic) units were synthesized by RAFT polymerization...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301453g
更新日期：2012-12-10 00:00:00
abstract：:The ability to adopt at least two different stable conformations is a common feature of proteins involved in many neurodegenerative diseases. The involved molecules undergo a conformational transition from native, mainly helical states to insoluble amyloid structures that have high β-sheet content. A detailed characte...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200587m
更新日期：2011-08-08 00:00:00
abstract：:This paper describes the synthesis and characterization of a novel organic polymer coating for the prevention of the growth of Pseudomonas aeruginosa on the solid surface of three-dimensional objects. Substrata were encapsulated with polyterpenol thin films prepared from terpinen-4-ol using radio frequency plasma enha...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100369n
更新日期：2010-08-09 00:00:00
abstract：:A simple, sequential approach for creation of hydrolytically degradable poly(ethylene glycol) (PEG) hydrogels has been developed and characterized. The chemistry involves an initial step growth polymerization reaction between PEG-diacrylate and dithiothreitol (DTT) to form acrylate-terminated (-PEG-DTT-)n PEG chains, ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701179s
更新日期：2008-03-01 00:00:00
abstract：:Placing a physical barrier between the injured site and the adjacent tissues is a very common and highly effective approach to prevent abdominal adhesions in these days. A biodegradable trilayered barrier was fabricated to prevent formation of abdominal adhesions, in which a poly(lactide-co-glycolide)/poly(lactide)-b-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01099
更新日期：2015-09-14 00:00:00
abstract：:Neutron reflectometry was used to investigate the time-dependent beta-casein adsorption at the silica-aqueous solution interface. The transient and steady-state structural characteristics of the adsorbed layer were determined from reflectivity curves, fitted to three-layer and two-layer models. The results show that t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0155221
更新日期：2001-10-01 00:00:00
abstract：:A comprehensive knowledge of the in vivo fate of polymers is essential for their potential application in humans. In this study, the body distribution, accumulation, and elimination processes of intraperitoneally (ip) administered poly(vinyl alcohol) (PVA) in mice were investigated in detail. Two derivatives of PVA (1...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200899h
更新日期：2011-10-10 00:00:00
abstract：:Inulin, the polydisperse polyfructose, extracted from chicory, has been modified via transesterification, using fatty acid methyl esters (FAME). The grafting of an alkyl chain onto the inulin backbone under different conditions for the development of potential tensio-active derivatives is described. The modification o...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049869q
更新日期：2004-09-01 00:00:00
abstract：:This paper introduces a chitosan microneedle patch for efficient and sustained transdermal delivery of hydrophilic macromolecules. Chitosan microneedles have sufficient mechanical strength to be inserted in vitro into porcine skin at approximately 250 μm in depth and in vivo into rat skin at approximately 200 μm in de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301293d
更新日期：2012-12-10 00:00:00
abstract：:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore electrospinning for protein-based bi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025581u
更新日期：2002-11-01 00:00:00
abstract：:The design and application of soft nanocomposite injectable hydrogels containing entrapped microgels for small-molecule drug delivery is demonstrated. Copolymer microgels based on N-isopropylacrylamide and acrylic acid were synthesized that exhibited both ionic and hydrophobic affinity for binding to bupivacaine, a ca...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201170h
更新日期：2011-11-14 00:00:00
abstract：:A shortage of available organ donors has created a need for engineered tissues. In this context, polymer-based hydrogels that break down inside the body are often used as constructs for growth factors and cells. Herein, we report imine cross-linked gels where degradation is controllable by the introduction of mixed im...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00519
更新日期：2015-07-13 00:00:00
abstract：:The structure of an extracellular polysaccharide (EPS) from Streptococcus thermophilus THS has been determined. A combination of component analysis, methylation analysis and NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by two-dimensi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0496514
更新日期：2005-01-01 00:00:00
abstract：:A challenge in the development of multifunctional drug delivery systems is to establish a reasonable and effective synthetic route for multifunctional polymer preparation. Herein, we propose a unique protocol to prepare multifunctional micelles by a cross-assembly process using three different functional polyurethanes...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00375
更新日期：2016-06-13 00:00:00
abstract：:Liver glycogen, a highly branched polymer of glucose, is important for maintaining blood-glucose homeostasis. It was recently shown that db/db mice, a model for Type 2 diabetes, are unable to form the large composite glycogen α particles present in normal, healthy mice. In this study, the structure of healthy mouse-li...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401714v
更新日期：2014-02-10 00:00:00
abstract：:A one-pot synthetic methodology for fabricating stimuli-responsive hemicellulose-based hydrogels was developed that consists of the in situ formation of magnetic iron oxide (Fe3O4) nanoparticles during the covalent cross-linking of O-acetyl-galactoglucomannan (AcGGM). The Fe3O4 nanoparticle content controlled the ther...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00801
更新日期：2015-08-10 00:00:00
abstract：:As a programmable biopolymer, DNA has shown great potential in the fabrication and construction of nanometer-scale assemblies and devices. In this report, we described a strategy for efficient manipulation of gold nanoparticle-bound DNA using restriction endonuclease. The digestion efficiency of this restriction enzym...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060517o
更新日期：2006-11-01 00:00:00
abstract：:The application of nanoparticles comprising amphiphilic block copolymers for the delivery of drugs is a subject of great interest as they hold promise for more effective and selective therapies. In order to achieve this ambition, it is of critical importance to develop our understanding of the self-assembly mechanisms...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00169
更新日期：2020-06-08 00:00:00
abstract：:Single-molecule measurements of the interaction of leukocyte function-associated antigen-1 (LFA-1), expressed on Jurkat T cells, with intercellular adhesion molecules-1 and -2 (ICAM-1 and ICAM-2) were conducted using atomic force microscopy (AFM). The force spectra (i.e., unbinding force versus loading rate) of both t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060559c
更新日期：2006-11-01 00:00:00
