Abstract:
:Aggregation phenomena in aqueous solutions of purified human tracheobronchial mucin have been studied by rheological methods, steady-state fluorescence, quasielastic light scattering, and spin probe techniques. At temperatures below 30 degrees C and concentrations above 15 mg/mL and in the absence of chaotropic agents, mucin solutions are viscoelastic gels. A gel-sol transition is observed at temperatures above 30 degrees C that is manifested by the diminishing storage modulus and a loss tangent above unity throughout the studied frequency range of the oscillatory shear. No decline in the mucin molecular weight is observed by size-exclusion chromatography above 30 degrees C in the absence of redox agents or proteolytic enzymes. Aggregation of hydrophobic protein segments of the mucin chains at 37 degrees C is indicated by QELS experiments. The decreasing polarity of the microenvironment of pyrene solubilized into mucin solutions at temperatures above 30 degrees C, concomitant with the gel-sol transition, shows the hydrophobicity of the formed aggregates. ESR spectra of the fatty acid spin probe, 16-doxylstearic acid indicate that the aggregate-aqueous interface becomes more developed at elevated temperatures.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Bromberg LE,Barr DPdoi
10.1021/bm005532mkeywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
325-34issue
3eissn
1525-7797issn
1526-4602journal_volume
1pub_type
杂志文章abstract::To broaden the application scope of lactic acid polymers, a new miniemulsion of poly(alkyl methacrylate-graft-lactic acid)s has been developed. The graft copolymer synthesis was based on a poly(lactic acid) macromonomer having a methacryloyl polymerizable group. The macromonomer and a comonomer of n-butyl methacrylate...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9007937
更新日期:2009-10-12 00:00:00
abstract::The long-term stability and success of orthopedic implants depend on the osseointegration process, which is strongly influenced by the biomaterial surface. A promising approach to enhance implant integration involves the modification of the surface of the implant by means of polymers that mimic the natural components ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3002683
更新日期:2012-05-14 00:00:00
abstract::Herein, we developed a new gene delivery vector by grafting a betaine monomer (N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate, DMAAPS) onto 25 KDa polyethylenimine (PEI 25K) via the Michael addition reaction. The graft ratio for betaine on PEI polymer could be readily controlled, and in this study three PEI-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301826m
更新日期:2013-03-11 00:00:00
abstract::The storage and transport of cells is a fundamental technology which underpins cell biology, biomaterials research, and emerging cell-based therapies. Inspired by antifreeze and ice-binding proteins in extremophiles, macromolecular (polymer) cryoprotectants are emerging as exciting biomaterials to enable the reduction...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00591
更新日期:2020-07-13 00:00:00
abstract::The oxidation reaction of regenerated cellulose fibers mediated by N-hydroxyphthalimide (NHPI) and various cocatalysts at room temperature for different time intervals and various amounts of low concentration sodium hypochlorite solution has been investigated to produce oxidized cellulose (OC), a biocompatible and bio...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9004854
更新日期:2009-08-10 00:00:00
abstract::We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200780r
更新日期:2011-10-10 00:00:00
abstract::Glycogen is mainly found as the principal storage form of glucose in cells. Many bacteria are able to synthesize large amounts of glycogen under unfavorable life conditions. By combining infrared spectroscopy, single molecule force spectroscopy (SMFS) and immuno-staining technique, we evidenced that planktonic P. flu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300497c
更新日期:2012-07-09 00:00:00
abstract::The polymerization of N-isopropylacrylamide (NIPAAm) with ionizable monomers results in pH-responsive lower critical solution temperature (LCST) polymer which works in an ionization-dependent manner. However, gradual ionization of the comonomer occurs at a broad pH range due to the electrostatic field generated by the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01465
更新日期:2019-04-08 00:00:00
abstract::Visible light-induced hydrogelation is attractive for various biomedical applications. In this study, hydrogels of alginate with phenolic hydroxyl groups (Alg-Ph) were obtained by irradiating a solution containing the polymer, ruthenium II trisbipyridyl chloride ([Ru(bpy)3]2+) and sodium persulfate (SPS), with visible...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01827
更新日期:2018-02-12 00:00:00
abstract::Block copolypeptides, which combine the self-assembly of block copolymers and the highly ordered 3D structures of proteins, are potential candidates for novel supramolecular structures and biotech applications, such as biosensors, tissue engineering, and selective drug delivery. The synthesis of model block copolypept...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0497217
更新日期:2004-09-01 00:00:00
abstract::Articular cartilage defects, caused by trauma, osteoarthritis, or other diseases, always lead to severe joint pain and joint dysfunction. Without access to progenitor cells and the supply of blood and nutrients, the impaired articular cartilage would be short of the capability to self-repair. Although the present clin...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/acs.biomac.9b00043
更新日期:2019-04-08 00:00:00
abstract::Peptide self-assembly is a facile route to the development of bioorganic hybrid materials that have sophisticated nanostructures toward diverse applications. Here, we report the synthesis of self-assembled peptide (Fmoc-diphenylalanine, Fmoc-FF)/graphitic carbon nitride (g-C3N4) hydrogels for light harvesting and biom...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00889
更新日期:2017-11-13 00:00:00
abstract::The aim of this investigation was to understand and engineer the interactions between endothelial cells and the electrospun (ES) polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) nanofiber surfaces and evaluate their potential for endothelialization. Elastomeric PVDF-HFP samples were electrospun to evaluate th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401825c
更新日期:2014-04-14 00:00:00
abstract::The first step in the conventional approach to self-assembled biomaterials is to develop well-defined nanostructures in vitro, which is followed by disruption of the preformed nanostructures at the inside of the cell to achieve bioactivity. Here, we propose an inverse strategy to develop in-cell gain-of-function self-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01816
更新日期:2017-03-13 00:00:00
abstract::Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are biopolyesters reported to provide favorable microenvironments for cell culture and possess potential for tissue engineering applications. Both biopolymers have been investigated for applications in a variety of medical scenarios,...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1007579
更新日期:2010-10-11 00:00:00
abstract::Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment. The starting suspensions used at ∼6 wt % CNC separated in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501629g
更新日期:2014-12-08 00:00:00
abstract::Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049941k
更新日期:2004-07-01 00:00:00
abstract::pH-dependent release from monoolein (MO) cubic phase was obtained by taking advantage of complex coacervation between hydrophobically modified alginate (HmAL) and hydrophobically modified silk fibroin (HmSF) in the water channels. The degree of coacervation was investigated at pH 3.0 by a light scattering method and t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101249e
更新日期:2011-02-14 00:00:00
abstract::Dual-cross-linked network (DCN) hydrogels with multiresponsive and self-healing properties are attracting intensive interests due to their enhanced mechanical strength for a wide range of applications. Herein, we developed a DCN hydrogel that combines a dynamic imine and a benzoxaboronic ester with a neutral pKa value...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01548
更新日期:2020-12-15 00:00:00
abstract::Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900889b
更新日期:2010-01-11 00:00:00
abstract::Polymer conjugation increases an enzyme's circulation time and stability for use as a therapeutic agent, but this attachment indubitably affects its properties. Covalent attachment of multiple polyethylene glycol chains with sizes of either 2, 5, 10, or 20 kDa increases the molecular weight and hydrodynamic radius of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1006954
更新日期:2010-12-13 00:00:00
abstract::Covalently modified albumin (BSA) microparticles were developed for potential use as an adjuvant in mucosal vaccines against hepatitis B. To synthesize consistent protein particles, a covalent approach was proposed to modify BSA. Our strategy was to bond maleic anhydride (MA) molecules to BSA structure by nucleophilic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400859z
更新日期:2013-09-09 00:00:00
abstract::Combining poly(ethylene glycol) (PEG) with sequence-defined peptides in PEG-peptide conjugates offers opportunities to realize next-generation drug formulation additives for overcoming undesired pharmacological profiles of difficult small molecule drugs. The tailored peptide segments provide sequence-specific, noncova...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00961
更新日期:2015-10-12 00:00:00
abstract::For efficient reversal of multidrug resistance (MDR) in chemotherapy for breast cancer, multifunctional self-assembled nanoparticles (MSN) based on a new amphiphilic copolymer consisting of bioreducible poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-tetraethylenepentamine] and polycaprolactone (PBD-PCL) were constru...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400378x
更新日期:2013-07-08 00:00:00
abstract::A wool fiber sample was submitted to chemical-free steam explosion in view of potential exploitation of keratin-based industrial and farm wastes. Fiber keratin was converted into a dark-yellow sludge that was submitted to phase separation by filtration, centrifugation, and precipitation of the soluble materials from t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060597w
更新日期:2006-12-01 00:00:00
abstract::Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or kappa-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by ce...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8002754
更新日期:2008-08-01 00:00:00
abstract::This study is devoted to developing amphiphilic block polymers based on phenylborate ester, which can self-assemble to form nanoparticles, as a glucose-sensitive drug carrier. Poly(ethylene glycol)-block-poly[(2-phenylboronic esters-1,3-dioxane-5-ethyl) methylacrylate] (MPEG5000-block-PBDEMA) was fabricated with MPEG5...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3003286
更新日期:2012-06-11 00:00:00
abstract::Ambidextrous supergelators are developed through structure-gelation screening of rationally designed cyclic dipeptides (CDPs). The organo- and hydrogels of CDPs were thoroughly characterized by their minimal gelation concentration (MGC) for organic and aqueous solvents, thermal stability (Tg), and viscoelastic propert...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00924
更新日期:2017-11-13 00:00:00
abstract::Here we present time-resolved in situ transmission electron microscopy (TEM) observations and real-time manipulation of nematic ordered cellulose and ultradrawn polyethylene films. Drawn films of these two polymers exhibited a unique response to the low-dose electron beam. Electron beam damage was minimal based on ret...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060546z
更新日期:2007-01-01 00:00:00
abstract::This paper reports the synthesis of hyaluronan (HA) and its derivatives via the hyaluronidase-catalyzed polymerization of 2-substituted oxazoline derivative monomers designed as "transition-state analogue substrates". Polymerization of 2-methyl oxazoline monomer from N-acetylhyalobiuronate (GlcAbeta(1-->3)GlcNAc) effe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049280r
更新日期:2005-03-01 00:00:00