Abstract:
:Visible light-induced hydrogelation is attractive for various biomedical applications. In this study, hydrogels of alginate with phenolic hydroxyl groups (Alg-Ph) were obtained by irradiating a solution containing the polymer, ruthenium II trisbipyridyl chloride ([Ru(bpy)3]2+) and sodium persulfate (SPS), with visible light. The hydrogelation kinetics and the mechanical properties of the resultant hydrogels were tunable by controlling the intensity of the light and the concentrations of [Ru(bpy)3]2+ and SPS. With appropriate concentrations of [Ru(bpy)3]2+ and SPS, the hydrogel could be obtained following approximately 10 s of irradiation using a normal desktop lamp. The hydrogelation process and the resultant hydrogel were cytocompatible; mouse fibroblast cells enclosed in the Alg-Ph hydrogel maintained more than 90% viability for 1 week. The solution containing Alg-Ph, [Ru(bpy)3]2+ and SPS was useful as a bioink for stereolithographic bioprinting. Cell-laden hydrogel constructs could be printed using the bioprinting system equipped with a visible light projector without a significant decrease in cell viability in the presence of photoabsorbent Acid Red 18. The hydrogel construct including a perfusable helical lumen of 1 mm in diameter could be fabricated using the printing system. These results demonstrate the significant potential of this visible light-induced hydrogelation system and the stereolithographic bioprinting using the hydrogelation system for tissue engineering and regenerative medicine.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Sakai S,Kamei H,Mori T,Hotta T,Ohi H,Nakahata M,Taya Mdoi
10.1021/acs.biomac.7b01827subject
Has Abstractpub_date
2018-02-12 00:00:00pages
672-679issue
2eissn
1525-7797issn
1526-4602journal_volume
19pub_type
杂志文章abstract::An artificial spinning system using regenerated silk fibroin solutions is adopted to produce high-performance silk fibers. In previous studies, alcohol-based agents, such as methanol or ethanol, were used to coagulate silk dope solutions, producing silk fiber with poor mechanical properties compared with those of nati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00232
更新日期:2018-06-11 00:00:00
abstract::Solid-state NMR is especially useful when the structures of peptides and proteins should be analyzed by taking into account the structural distribution, that is, the distribution of the torsion angle of the individual residue. In this study, two-dimensional spin-diffusion solid-state NMR spectra of 13C-double-labeled ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0600522
更新日期:2006-04-01 00:00:00
abstract::Cellulose is the most familiar and most abundant strong biopolymer, but the reasons for its outstanding mechanical performance are not well understood. Each glucose unit in a cellulose chain is joined to the next by a covalent C-O-C linkage flanked by two hydrogen bonds. This geometry suggests some form of cooperativi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401616n
更新日期:2014-03-10 00:00:00
abstract::A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based on three different core sizes (18, 35, 65 nm) and five polymer...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300248u
更新日期:2012-05-14 00:00:00
abstract::The interaction of neuropeptides, vasoactive intestinal peptide (VIP), or growth hormone-releasing hormone (GHRH), with a cationic carbosilane dendrimer forms dendriplexes with antitumoral behavior in advanced prostate cancer cells PC3. At the concentrations used for dendriplexes formation, the free peptides were prot...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01632
更新日期:2019-03-11 00:00:00
abstract::Inspired by tea stains, plant polyphenolic tannic acid (TA) was beneficially employed as the primer anchor for functional polymer brushes. The brominated TA (TABr) initiator primer was synthesized by partial modification of TA with alkyl bromide functionalities. TABr with trihydroxyphenyl moieties can readily anchor o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501623c
更新日期:2015-03-09 00:00:00
abstract::Incorporating charged amino acid side chains in polypeptide polymer backbones to improve solubility usually leads to reduced secondary structuring. Here we show that highly water soluble (>15 mg.mL-1) β-sheets can be obtained via nucleotide monophosphate grafting onto simple poly(γ-propargyl- L-glutamate) backbone. Th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01084
更新日期:2018-10-08 00:00:00
abstract::Glycerol derivatives of cutin and suberin monomers were synthesized by acid catalysis. Their dispersion in an aqueous solution was examined by phase contrast microscopy, neutron scattering, and solid state NMR. It is shown that the phase behavior strongly depends on the nature of the derivatives forming either lumps o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049325o
更新日期:2005-01-01 00:00:00
abstract::A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell line...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00776
更新日期:2016-10-10 00:00:00
abstract::Major ampullate silk, also known as dragline silk, is one of the strongest biomaterials known. This silk is composed of two proteins, major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2). Only partial cDNA sequences have been obtained for these proteins, and these sequences are toward the C-termin...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050472b
更新日期:2005-11-01 00:00:00
abstract::Biodegradable polyrotaxanes (PRXs) were synthesized from bis-amino-terminated poly(L-lactide) (PLLA) and alpha-cyclodextrin (alpha-CD), combined with capping by bulky end groups at the amino-termini of PLLA through enzymatically cleavable peptide linkages. The crystalline structure of the PRXs in the solid state was i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900440v
更新日期:2009-08-10 00:00:00
abstract::Cocontinuous phase structures of immiscible polymers can be developed under appropriate melt-blending conditions. Because of the presence of interfacial tension, such cocontinuous structures start to coarsen when heated to a temperature higher than the melting/softening temperature of both phases. In this study, a met...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900008d
更新日期:2009-05-11 00:00:00
abstract::We have found that dialysis of 5 mg/mL collagen solution into the phosphate solution with a pH of 7.1 and an ionic strength of 151 mM [corrected] at 25 °C results in a collagen gel with a birefringence and tubular pores aligned parallel to the growth direction of the gel. The time course of averaged diameter of tubula...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200869p
更新日期:2012-01-09 00:00:00
abstract::Glycosaminoglycans (GAGs) and glycoproteins are vital components of the extracellular matrix, directing cell proliferation, differentiation, and migration and tissue homeostasis. Here, we demonstrate supramolecular GAG-like glycopeptide nanofibers mimicking bioactive functions of natural hyaluronic acid molecules. Sel...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01669
更新日期:2016-02-08 00:00:00
abstract::Bioprinting has become an important tool for fabricating regenerative implants and in vitro cell culture platforms. However, until today, extrusion-based bioprinting processes are limited to resolutions of hundreds of micrometers, which hamper the reproduction of intrinsic functions and morphologies of living tissues....
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01577
更新日期:2021-01-07 00:00:00
abstract::The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5% w/w gelatin concentration; 15, 20, and 25...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008487
更新日期:2010-12-13 00:00:00
abstract::Periodic patterns resembling spirals were observed to form spontaneously upon unassisted cooling of d-glucaric acid- and d-galactaric acid-based polyamide solutions in N-methyl-N-morpholine oxide (NMMO) monohydrate. Similar observations were made in d-galactaric acid-based polyamide/ionic liquid (IL) solutions. The mo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00977
更新日期:2015-09-14 00:00:00
abstract::Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009075
更新日期:2011-09-12 00:00:00
abstract::In this contribution, a hydrophobically modified polysaccharide derivative is synthesized in an eco-friendly solvent water by conjugation of benzylamine with the backbone of the biopolymer. Owing to the presence of aromatic moieties, the resulting water-soluble polysaccharide derivative self-assembles spontaneously an...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00638
更新日期:2015-08-10 00:00:00
abstract::Many dental restorative dental composites still utilize 2,2-bis[4-(2-hydroxy-3-methacryloyloxy propoxy) phenyl] propane (Bis-GMA) as base resin. The high viscosity of Bis-GMA necessitates dilution with dimethacrylate ethers of low viscosity such as triethylene glycol dimethacrylate (TEGDMA). However, increased amounts...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050491l
更新日期:2006-01-01 00:00:00
abstract::As a means to stimulate wound healing, a hollow fiber membrane system might be placed within a wound bed to provide local and externally regulated controlled delivery of regenerative factors. After sufficient healing, it would be desirable to triggerably degrade these fibers as opposed to pulling them out. Accordingly...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500552f
更新日期:2014-08-11 00:00:00
abstract::Covalently modified albumin (BSA) microparticles were developed for potential use as an adjuvant in mucosal vaccines against hepatitis B. To synthesize consistent protein particles, a covalent approach was proposed to modify BSA. Our strategy was to bond maleic anhydride (MA) molecules to BSA structure by nucleophilic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400859z
更新日期:2013-09-09 00:00:00
abstract::Cell cultivation on the surface of a novel poly(N-isopropylacrylamide) (PNIPA) hydrogel (N-NC gel), consisting of a specific type of organic (PNIPA)/inorganic (clay) network, was studied using three cell types; HepG2 human hepatoma cells, human dermal fibroblasts, and human umbilical vein endothelial cells. For the fi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060549b
更新日期:2006-11-01 00:00:00
abstract::Coatings derived from surface active block copolymers (SABCs) having a combination of hydrophobic aliphatic (linear hydrocarbon or propylene oxide-derived groups) and hydrophilic poly(ethlyene glycol) (PEG) side chains have been developed. The coatings demonstrate superior performance against protein adsorption as wel...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300363g
更新日期:2012-06-11 00:00:00
abstract::C13-dipeptides that did not gel on their own were found to form hydrogels when combined with mixtures (coassembly). At pH = 4.6, by mixing negatively charged C13-WD (C13-WD2- and/or C13-WD-) with C13-KW or C13-YK, where the side chain of K carried positive charge, two composite hydrogels with different mechanical prop...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01325
更新日期:2020-12-14 00:00:00
abstract::We show that functionalizing polymer-coated colloidal nanoplatelets with guanosine groups allows synergistic increase of mechanical properties in nacre-mimetic lamellar self-assemblies. Anionic montmorillonite (MTM) was first coated using cationic poly(diallyldimethylammonium chloride) (PDADMAC) to prepare core-shell ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400056c
更新日期:2013-08-12 00:00:00
abstract::Electrospun natural polymer membranes were fabricated from collagen or gelatin coated with a bioactive recombinant fragment of perlecan, a natural heparan sulfate proteoglycan. The electrospinning process allowed the facile processing of a three-dimensional, porous fibril (2-6 microm in diameter) matrix suitable for t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm061003s
更新日期:2007-04-01 00:00:00
abstract::Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801430x
更新日期:2009-05-11 00:00:00
abstract::The shear rheology of a microcrystalline cellulose dissolved in a 9% NaOH aqueous solution was studied in the steady and oscillatory modes. The cellulose-(9% NaOH-H(2)O) mixtures show not to be true solutions. In the dilute regime, with cellulose concentration below 1%, the rheological behavior is typical of the one o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm020100s
更新日期:2003-03-01 00:00:00
abstract::Eosinophil cationic protein (ECP) is an antimicrobial protein belonging to the superfamily of RNase A. ECP exhibits a broad spectrum of action against bacteria and, at higher concentrations, displays cytotoxic activity to eukaryotic cells. Recently, a powerful aggregation activity for lipid vesicles and for the gram-n...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100334u
更新日期:2010-08-09 00:00:00