Abstract：
:Cell cultivation on the surface of a novel poly(N-isopropylacrylamide) (PNIPA) hydrogel (N-NC gel), consisting of a specific type of organic (PNIPA)/inorganic (clay) network, was studied using three cell types; HepG2 human hepatoma cells, human dermal fibroblasts, and human umbilical vein endothelial cells. For the first time, it was found that cells could be cultured to be confluent on the surfaces of PNIPA hydrogels using N-NC gels, regardless of gel thickness. Cell adhesion and proliferation on N-NC gels exhibit strong dependencies on clay concentration (C(clay)), and the numbers of cultured cells are maximum at about C(clay) = 6 x 10(-2) mol (45.72 g)/1 L of H(2)O. On the contrary, it was almost impossible to culture cells on conventional, chemically crosslinked PNIPA hydrogels, regardless of their cross-linker concentration. The reasons why cells cultured only on the surfaces of N-NC gels with their specific network structure and composition were discussed in terms of water content, protein adsorption, surface flatness, hydrophobicity of dehydrated PNIPA chains, and the anionic charge on exfoliated clay. Finally, it was found that cells cultured on the surfaces of N-NC gels could be detached in the forms of sheets of cells without trypsin treatment, but by just decreasing the temperature to 20 degrees C.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Haraguchi K,Takehisa T,Ebato Mdoi
10.1021/bm060549bsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
3267-75issue
11eissn
1525-7797issn
1526-4602journal_volume
7pub_type
杂志文章abstract：:Enzymatic hydrolysis of selected copolymers of cellulose diacetate-graft-poly(L-lactide)s (CDA-g-PLLAs) were conducted with proteinase K for film specimens, which were solely quenched from the molten state or, further, annealed at temperatures below or above their glass transition temperatures. The hydrolysis rates de...
journal_title：Biomacromolecules
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更新日期：2011-01-10 00:00:00
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更新日期：2008-04-01 00:00:00
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