Abstract:
:In this contribution, a hydrophobically modified polysaccharide derivative is synthesized in an eco-friendly solvent water by conjugation of benzylamine with the backbone of the biopolymer. Owing to the presence of aromatic moieties, the resulting water-soluble polysaccharide derivative self-assembles spontaneously and selectively from solution on the surface of nanometric thin films and sheets of polystyrene (PS). The synthetic polymer modified in this way bears a biocompatible nanolayer suitable for the immobilization of horseradish peroxidase (HRP), a heme-containing metalloenzyme often employed in biocatalysis and biosensors. Besides the detailed characterization of the polysaccharide derivative, a quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM) are used to investigate the binding efficiency and interaction of HRP with the tailored polysaccharide interfaces. Subsequent enzyme activity tests reveal details of the interaction of HRP with the solid support. The novel polysaccharide derivative and its use as a material for the selective modification of PS lead to a beneficial, hydrophilic environment for HRP, resulting in high enzymatic activities and a stable immobilization of the enzyme for biocatalytic and analytic purposes.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Mohan T,Rathner R,Reishofer D,Koller M,Elschner T,Spirk S,Heinze T,Stana-Kleinschek K,Kargl Rdoi
10.1021/acs.biomac.5b00638subject
Has Abstractpub_date
2015-08-10 00:00:00pages
2403-11issue
8eissn
1525-7797issn
1526-4602journal_volume
16pub_type
杂志文章abstract::Cellular uptake and intracellular targeting to specific organelles are key events in the cellular processing of nanomaterials. Herein, we perform a detailed structure-property relationship study on carboxylic acid-side-chain-bearing polyacrylates to provide design criteria for the manipulation of their cellular intera...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00244
更新日期:2020-08-10 00:00:00
abstract::The ability to direct neurite growth into a close proximity of stimulating elements of a neural prosthesis, such as a retinal or cochlear implant (CI), may enhance device performance and overcome current spatial signal resolution barriers. In this work, spiral ganglion neurons (SGNs), which are the target neurons to b...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501019s
更新日期:2014-10-13 00:00:00
abstract::The morphology of silk produced by recluse spiders (Loxosceles arizonica) was investigated by scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. This silk consisted entirely of very long, thin ribbons of width 2-4 microm and thicknesses of no more than 40 nm. The correspondenc...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm010108m
更新日期:2002-01-01 00:00:00
abstract::Given the significance of hydrogels as cell-instructive materials, it is important to understand how differences in their chemical and physical properties are able to direct cell fate. For example, it remains unclear how different hydrogel cross-linking chemistries and gelation mechanisms influence cell behavior. Here...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01740
更新日期:2017-03-13 00:00:00
abstract::A novel methacrylate monomer containing a quinolone moiety was synthesized and homopolymerized in N,N-dimethylformamide (DMF) by using azobisisobutyronitrile (AIBN) as an initiator. The new monomer was copolymerized with poly(ethylene glycol) methyl ether methacrylate (MPEGMA) in DMF using the same initiator. The mono...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049383+
更新日期:2005-01-01 00:00:00
abstract::This investigation describes the formulation and characterization of rheologically structured vehicles (RSVs) designed for improved drug delivery to the vagina. Interactive, multicomponent, polymeric platforms were manufactured containing hydroxyethylcellulose (HEC, 5% w/w) polyvinylpyrrolidone (PVP, 4% w/w), Pluronic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9003332
更新日期:2009-09-14 00:00:00
abstract::This paper describes the synthesis and evaluation of a series of multifunctional poly-l-glutamic acid derivatives that can be used as vectors for gene delivery. They readily form polyelectrolyte complexes with DNA, resulting in a reduced surface charge and size of the DNA. The formation of a polymer-DNA complex and th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034014j
更新日期:2003-09-01 00:00:00
abstract::Dual-cross-linked network (DCN) hydrogels with multiresponsive and self-healing properties are attracting intensive interests due to their enhanced mechanical strength for a wide range of applications. Herein, we developed a DCN hydrogel that combines a dynamic imine and a benzoxaboronic ester with a neutral pKa value...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01548
更新日期:2020-12-15 00:00:00
abstract::Their physicochemical properties and relatively low cost make cellulose nanocrystals (CNCs) a potential candidate for future large-scale production in many fields including nanomedicine. Prior to a sustained and responsible development as theranostic agents, robust and reliable data concerning their safety, biocompati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00805
更新日期:2015-09-14 00:00:00
abstract::Selectively spooling single plasmid DNA (pDNA), as a giant polyelectrolyte, into a nanosized toroidal structure or folding it into a rod-like structure has been accomplished by polyion complexation with block catiomers to form polymeric micelles in varying NaCl concentrations. The interactive potency between the pDNA ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00491
更新日期:2015-09-14 00:00:00
abstract::Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are biopolyesters reported to provide favorable microenvironments for cell culture and possess potential for tissue engineering applications. Both biopolymers have been investigated for applications in a variety of medical scenarios,...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1007579
更新日期:2010-10-11 00:00:00
abstract::Since their discovery at the end of the previous millennium, carbon nanotubes (CNTs) have been the object of thousands of papers describing their applications in fields ranging from physics to electronics, photonics, chemistry, biology, and medicine. The development of chemical approaches to modify their graphitic sid...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm201020h
更新日期:2011-10-10 00:00:00
abstract::Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009075
更新日期:2011-09-12 00:00:00
abstract::We synthesized extremely deformable red blood cell-like microgel particles and loaded them with bovine hemoglobin (Hb) to potentiate oxygen transport. With similar shape and size as red blood cells (RBCs), the particles were fabricated using the PRINT (particle replication in nonwetting templates) technique. Low cross...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3007242
更新日期:2012-09-10 00:00:00
abstract::Here we present time-resolved in situ transmission electron microscopy (TEM) observations and real-time manipulation of nematic ordered cellulose and ultradrawn polyethylene films. Drawn films of these two polymers exhibited a unique response to the low-dose electron beam. Electron beam damage was minimal based on ret...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060546z
更新日期:2007-01-01 00:00:00
abstract::In the present study we describe the synthesis, wet spinning, mechanical testing, and degradation of poly(urethane urea)s (PUURs) intended for clinical use in anterior cruciate ligament (ACL) reconstruction. The effects of soft segment chemical composition and molar mass and the kind of diamine chain extender on the m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025535u
更新日期:2002-09-01 00:00:00
abstract::Semicrystalline, thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOx) layers covalently bonded to glass or silica wafers were obtained via the surface-termination of the living polymer chains. Polymer solutions in acetonitrile were exposed to 50 °C for various time periods and were poured onto the functionalized sol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00745
更新日期:2015-09-14 00:00:00
abstract::Poly(dimethyl siloxane) (PDMS) is extensively used for biomedical applications due to its low cost, ease of fabrication, high durability and flexibility, oxygen permeability, and self-healing properties. PDMS, however, has some significant drawbacks. PDMS endures unacceptably high levels of nonspecific protein fouling...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300399s
更新日期:2012-05-14 00:00:00
abstract::Despite numerous strategies involving dynamic covalent bonds to produce self-healing hydrogels with similar frequency-dependent stiffness to native tissues, it remains challenging to use biologically relevant thiol/disulfide exchange to confer such properties to polymeric networks. Herein, we report a new method based...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00980
更新日期:2015-11-09 00:00:00
abstract::As an aim toward developing biologically mimetic and functional nanofiber-based tissue engineering scaffolds, we demonstrated the encapsulation of a model protein, fluorescein isothiocyanate-conjugated bovine serum albumin (fitcBSA), along with a water-soluble polymer, poly(ethylene glycol) (PEG), within the biodegrad...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050743i
更新日期:2006-04-01 00:00:00
abstract::The SEC-MALS-QELS (size-exclusion chromatography equipped with multiangle light scattering and quasi-elastic light scattering detectors) method using lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) and LiCl/1,3-dimethyl-2-imidazolidinone (LiCl/DMI) as mobile phases was applied to cellulose and cellulose tricarbanil...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049386m
更新日期:2005-05-01 00:00:00
abstract::Polymeric prodrugs of mPEG-PLA-haloperidol (methoxypoly(ethylene glycol)-b-poly(lactic acid)) can self-assemble into nanoscale micelle-like structures in aqueous solutions. mPEG-PLA-haloperidol was prepared and characterized using 1H and 13C NMR. The conjugation efficiency was found to be 64.8 +/- 21%. Micelles that f...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050188k
更新日期:2005-09-01 00:00:00
abstract::The formation of calcite prism architecture in the prismatic layer of the mollusk shell involves the participation of a number of different proteins. One protein family, Asprich, has been identified as a participant in amorphous calcium carbonate stabilization and calcite architecture in the prismatic layer of the mol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100738r
更新日期:2010-10-11 00:00:00
abstract::A novel poly(epsilon-caprolactone)-organosiloxane hybrid material containing calcium salt (Si-O-PCL) was prepared and evaluated as a bioactive and degradable bone substitute material. The Si-O-PCL hybrid was synthesized by the end-capping of 3-isocyanatopropyl triethoxysilane with alpha,omega-hydroxyl PCL following so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049885n
更新日期:2004-07-01 00:00:00
abstract::New therapeutic strategies for the treatment of neoplastic pathologies and, in particular, metastasis processes are based on the inhibitory effect of angiogenic processes. The present article deals with the design, preparation, and application of new "polymer drugs" with a clear inhibitory effect of the activation of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100223d
更新日期:2010-07-12 00:00:00
abstract::We present a new design of peptide-polymer conjugates where a polymer chain is covalently linked to the side chain of a helix bundle-forming peptide. The effect of conjugated polymer chains on the peptide structure was examined using a de novo designed three-helix bundle and a photoactive four-helix bundle. Upon attac...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800113g
更新日期:2008-08-01 00:00:00
abstract::Hydrogels have been widely explored for biomedical applications, with injectable hydrogels being of particular interest for their ability to precisely deliver drugs and cells to targets. Although these hydrogels have demonstrated satisfactory properties in many cases, challenges still remain for commercialization. In ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01148
更新日期:2016-11-14 00:00:00
abstract::A recombinant silk-elastin-like protein copolymer SELP-47K containing tandemly repeated amino acid sequence blocks from silk, GAGAGS, and elastin, GVGVP, was fabricated into microdiameter fibers using a wet-spinning technique. Raman spectral analysis revealed the formation of antiparallel beta-sheet crystals of the si...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801296r
更新日期:2009-03-09 00:00:00
abstract::Bone-marrow-derived mesenchymal stem cells (BMSCs) possess vast potential for tissue engineering and regenerative medicine. In this study, an injectable hydrogel comprising poly(l-glutamic acid)-graft-tyramine (PLG-g-TA) with tunable microenvironment was developed via enzyme-catalyzed cross-linking and used as an arti...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00884
更新日期:2016-12-12 00:00:00
abstract::The formation of protein-polymer complexes was studied in an aqueous system using dynamic light scattering (DLS) and static light scattering (SLS) as the main experimental tools. Human serum albumin (HSA) was used as a protein and complexed with four representative water-soluble polymers: poly(N-isopropylacrylamide) (...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0341935
更新日期:2003-11-01 00:00:00