Abstract:
:We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO(2)), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by quartz crystal microbalance with dissipation (QCM-D). Complementary surface information is provided by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). QCM-D shows no mass increases to any of the FP-coated surfaces upon treating with Fg or HSA. Also, when Fg- or HSA-coated surfaces are exposed to the FPs, a significant increase in adsorbed mass occurs because the FPs are highly surface-active displacing Fg. Additionally, fluorescence microscopy confirms that very little Fg adsorbs to the FP-coated surfaces. We propose that FP coatings prevent protein adsorption by steric stabilization and could be an alternative method for preventing unwanted bioadhesion on medical materials.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Pillai S,Arpanaei A,Meyer RL,Birkedal V,Gram L,Besenbacher F,Kingshott Pdoi
10.1021/bm900589rsubject
Has Abstractpub_date
2009-10-12 00:00:00pages
2759-66issue
10eissn
1525-7797issn
1526-4602journal_volume
10pub_type
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