Abstract:
:These studies illustrate synthetic paths to covalently attach T1 and Φ11 bacteriophages (phages) to inert polymeric surfaces while maintaining the bacteriophage's biological activities capable of killing deadly human pathogens. The first step involved the formation of acid (COOH) groups on polyethylene (PE) and polytetrafluoroethylene (PTFE) surfaces using microwave plasma reactions in the presence of maleic anhydride, followed by covalent attachment of T1 and Φ11 species via primary amine groups. The phages effectively retain their biological activity manifested by a rapid infection with their own DNA and effective destruction of Escherichia coli and Staphylococcus aureus human pathogens. These studies show that simultaneous covalent attachment of two biologically active phages effectively destroy both bacterial colonies and eliminate biofilm formation, thus offering an opportunity for an effective combat against multibacterial colonies as well as surface detections of other pathogens.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Pearson HA,Sahukhal GS,Elasri MO,Urban MWdoi
10.1021/bm400290usubject
Has Abstractpub_date
2013-05-13 00:00:00pages
1257-61issue
5eissn
1525-7797issn
1526-4602journal_volume
14pub_type
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journal_title:Biomacromolecules
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