Phage-bacterium war on polymeric surfaces: can surface-anchored bacteriophages eliminate microbial infections?


:These studies illustrate synthetic paths to covalently attach T1 and Φ11 bacteriophages (phages) to inert polymeric surfaces while maintaining the bacteriophage's biological activities capable of killing deadly human pathogens. The first step involved the formation of acid (COOH) groups on polyethylene (PE) and polytetrafluoroethylene (PTFE) surfaces using microwave plasma reactions in the presence of maleic anhydride, followed by covalent attachment of T1 and Φ11 species via primary amine groups. The phages effectively retain their biological activity manifested by a rapid infection with their own DNA and effective destruction of Escherichia coli and Staphylococcus aureus human pathogens. These studies show that simultaneous covalent attachment of two biologically active phages effectively destroy both bacterial colonies and eliminate biofilm formation, thus offering an opportunity for an effective combat against multibacterial colonies as well as surface detections of other pathogens.






Pearson HA,Sahukhal GS,Elasri MO,Urban MW




Has Abstract


2013-05-13 00:00:00












  • Cellulosic graft copolymer: poly(methyl methacrylate) with cellulose side chains.

    abstract::A cellulose macromonomer, N-(15-methacryloyloxypentadecanoyl)-tri-O-acetyl-beta-cellulosylamine (CTA-C15-MA (2); M(2)) with number averaged degree of polymerization (DP(n)) = 13), was copolymerized with methyl methacrylate (MMA; M(1)) to give cellulosic copolymer with CTA side chains, PMMA-g-(CTA-C15) (3-A). An absolu...


    pub_type: 杂志文章


    authors: Enomoto-Rogers Y,Kamitakahara H,Takano T,Nakatsubo F

    更新日期:2009-08-10 00:00:00

  • Poly(2-hydroxyethyl methacrylate) for enzyme immobilization: impact on activity and stability of horseradish peroxidase.

    abstract::On the basis of their versatile structure and chemistry as well as tunable mechanical properties, polymer brushes are well-suited as supports for enzyme immobilization. However, a robust surface design is hindered by an inadequate understanding of the impact on activity from the coupling motif and enzyme distribution ...


    pub_type: 杂志文章


    authors: Lane SM,Kuang Z,Yom J,Arifuzzaman S,Genzer J,Farmer B,Naik R,Vaia RA

    更新日期:2011-05-09 00:00:00

  • DNA condensation by chiral alpha-methylated polyamine analogues and protection of cellular DNA from oxidative damage.

    abstract::Polyamines are essential molecules supporting the structure, conformation, and function of nucleic acids and proteins. We studied stereoisomers of alpha,alpha'-dimethylated spermine [(R,R)-Me(2)Spm, (S,S)-Me(2)Spm, (R,S)-Me(2)Spm] for their ability to provoke DNA condensation and protect DNA from damage. (R,R)- and (R...


    pub_type: 杂志文章


    authors: Nayvelt I,Hyvönen MT,Alhonen L,Pandya I,Thomas T,Khomutov AR,Vepsäläinen J,Patel R,Keinänen TA,Thomas TJ

    更新日期:2010-01-11 00:00:00

  • Rheological study of genipin cross-linked chitosan hydrogels.

    abstract::This paper reports the rheological behavior of chitosan solutions that have been cross-linked with different amounts of genipin, at body temperature and physiological pH. The effect of the cross-linker loading on the rheological properties of hydrogels has been evaluated. The oscillatory time sweep method was used to ...


    pub_type: 杂志文章


    authors: Moura MJ,Figueiredo MM,Gil MH

    更新日期:2007-12-01 00:00:00

  • Nanostructured biocomposite scaffolds based on collagen coelectrospun with nanohydroxyapatite.

    abstract::Nanofibrous biocomposite scaffolds of type I collagen and nanohydroxyapatite (nanoHA) of varying compositions (wt %) were prepared by electrostatic cospinning. The scaffolds were characterized for structure and morphology by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic fo...


    pub_type: 杂志文章


    authors: Thomas V,Dean DR,Jose MV,Mathew B,Chowdhury S,Vohra YK

    更新日期:2007-02-01 00:00:00

  • Transiently Responsive Block Copolymer Micelles Based on N-(2-Hydroxypropyl)methacrylamide Engineered with Hydrolyzable Ethylcarbonate Side Chains.

    abstract::The lack of selectivity and low solubility of many chemotherapeutics impels the development of different biocompatible nanosized drug carriers. Amphiphilic block copolymers, composed of a hydrophilic and hydrophobic domain, show great potential because of their small size, large solubilizing power and loading capacity...


    pub_type: 杂志文章


    authors: Kasmi S,Louage B,Nuhn L,Van Driessche A,Van Deun J,Karalic I,Risseeuw M,Van Calenbergh S,Hoogenboom R,De Rycke R,De Wever O,Hennink WE,De Geest BG

    更新日期:2016-01-11 00:00:00

  • Multiblock copolymers of L-lactide and trimethylene carbonate.

    abstract::Sequential copolymerizations of trimethylene carbonate (TMC) and l-lactide (LLA) were performed with 2,2-dibutyl-2-stanna-1,3-oxepane as a bifunctional cyclic initiator. The block lengths were varied via the monomer/initiator and via the TMC/l-lactide ratio. The cyclic triblock copolymers were transformed in situ into...


    pub_type: 杂志文章


    authors: Pospiech D,Komber H,Jehnichen D,Häussler L,Eckstein K,Scheibner H,Janke A,Kricheldorf HR,Petermann O

    更新日期:2005-01-01 00:00:00

  • The unique ribbon morphology of the major ampullate silk of spiders from the genus Loxosceles (recluse spiders).

    abstract::The morphology of silk produced by recluse spiders (Loxosceles arizonica) was investigated by scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. This silk consisted entirely of very long, thin ribbons of width 2-4 microm and thicknesses of no more than 40 nm. The correspondenc...


    pub_type: 杂志文章


    authors: Coddington JA,Chanzy HD,Jackson CL,Raty G,Gardner KH

    更新日期:2002-01-01 00:00:00

  • Electrodissolution of inorganic ions/DNA multilayer film for tunable DNA release.

    abstract::A layer-by-layer film composed of DNA and inorganic zirconium ion (Zr(4+)) was fabricated on the surface of gold thin film, and an electric field triggered disintegration of the multilayer film was studied by using electrochemical surface plasmon resonance (EC-SPR). EC-SPR results demonstrated that the film was disass...


    pub_type: 杂志文章


    authors: Wang F,Li D,Li G,Liu X,Dong S

    更新日期:2008-10-01 00:00:00

  • Ultra-sub-stoichiometric "Dynamic" Bioconjugation Reduces Viscosity by Disrupting Immunoglobulin Oligomerization.

    abstract::Monoclonal antibodies (mAb) are a major focus of the pharmaceutical industry, and polyclonal immunoglobulin G (IgG) therapy is used to treat a wide variety of health conditions. As some individuals require mAb/IgG therapy their entire life, there is currently a great desire to formulate antibodies for bolus injection ...


    pub_type: 杂志文章


    authors: Gong Y,Soleymani Abyaneh H,Drossis N,Niederquell A,Kuentz M,Leroux JC,de Haan HW,Gauthier MA

    更新日期:2019-09-09 00:00:00

  • Microstructure and rheological behavior of pure and mixed pectin gels.

    abstract::The microstructure and the rheological properties of pure HM (high methoxyl) and LM (low methoxyl) pectin gels and of mixed HM/LM pectin gels have been investigated. Gel formation of either the HM or LM pectin, or both, was initiated in the mixed gels by varying the sucrose and Ca(2+) content. The microstructure was c...


    pub_type: 杂志文章


    authors: Löfgren C,Walkenström P,Hermansson AM

    更新日期:2002-11-01 00:00:00

  • Joining Two Natural Motifs: Catechol-Containing Poly(phosphoester)s.

    abstract::Numerous catechol-containing polymers, including biodegradable polymers, are currently heavily discussed for modern biomaterials. However, there is no report combining poly(phosphoester)s (PPEs) with catechols. Adhesive PPEs have been prepared via acyclic diene metathesis polymerization. A novel acetal-protected catec...


    pub_type: 杂志文章


    authors: Becker G,Ackermann LM,Schechtel E,Klapper M,Tremel W,Wurm FR

    更新日期:2017-03-13 00:00:00

  • Injectable Catalyst-Free Poly(Propylene Fumarate) System Cross-Linked by Strain Promoted Alkyne-Azide Cycloaddition Click Chemistry for Spine Defect Filling.

    abstract::A new PPF-BCN/hyPCL32-N3 injectable system that can be cross-linked by catalyst-free, strain promoted alkyne-azide cycloaddition (SPAAC) click chemistry was developed for tissue engineering applications. The system consisted of two components: PPF-BCN, poly(propylene fumarate) (PPF) functionalized with (1R,8S,9s)-bicy...


    pub_type: 杂志文章


    authors: Liu X,Miller AL 2nd,Xu H,Waletzki BE,Lu L

    更新日期:2019-09-09 00:00:00

  • Polymer micelle with pH-triggered hydrophobic-hydrophilic transition and de-cross-linking process in the core and its application for targeted anticancer drug delivery.

    abstract::In this study, an novel amphiphilic block copolymer P[PEGMA-b-(DEMA-co-APMA)]-FA and its cross-linker uracil-(CH2)6-uracil (U-(CH2)6-U) were synthesized and used as the targeted and pH-responsive nanocarriers for anticancer drug delivery. The hydrophobic block of the copolymer contains adenine (A) and tertiary amine m...


    pub_type: 杂志文章


    authors: Fan J,Zeng F,Wu S,Wang X

    更新日期:2012-12-10 00:00:00

  • Chain registry and load-dependent conformational dynamics of collagen.

    abstract::Degradation of fibrillar collagen is critical for tissue maintenance. Yet, understanding collagen catabolism has been challenging partly due to a lack of atomistic picture for its load-dependent conformational dynamics, as both mechanical load and local unfolding of collagen affect its cleavage by matrix metalloprotei...


    pub_type: 杂志文章


    authors: Teng X,Hwang W

    更新日期:2014-08-11 00:00:00

  • Grafting of oligosaccharides onto synthetic polymer colloids.

    abstract::A new method to form colloidally stable oligosaccharide-grafted synthetic polymer particles has been developed. The oligosaccharides, of weight-average degree of polymerization approximately 38, were obtained by enzymatic debranching of amylopectin. Through the use of a cerium(IV)-based redox initiation process, oligo...


    pub_type: 杂志文章


    authors: Mange S,Dever C,De Bruyn H,Gaborieau M,Castignolles P,Gilbert RG

    更新日期:2007-06-01 00:00:00

  • Collagenase-labile polyurethane urea synthesis and processing into hollow fiber membranes.

    abstract::As a means to stimulate wound healing, a hollow fiber membrane system might be placed within a wound bed to provide local and externally regulated controlled delivery of regenerative factors. After sufficient healing, it would be desirable to triggerably degrade these fibers as opposed to pulling them out. Accordingly...


    pub_type: 杂志文章


    authors: Fu HL,Hong Y,Little SR,Wagner WR

    更新日期:2014-08-11 00:00:00

  • Curcumin encapsulation in submicrometer spray-dried chitosan/Tween 20 particles.

    abstract::Optimal curcumin delivery for medicinal applications requires a drug delivery system that both solubilizes curcumin and prevents degradation. To achieve this, curcumin has been encapsulated in submicrometer chitosan/Tween 20 particles via a benchtop spray-drying process. Spray-drying parameters have been optimized usi...


    pub_type: 杂志文章


    authors: O'Toole MG,Henderson RM,Soucy PA,Fasciotto BH,Hoblitzell PJ,Keynton RS,Ehringer WD,Gobin AS

    更新日期:2012-08-13 00:00:00

  • Mechanism of Polymer-Mediated Cryopreservation Using Poly(methyl glycidyl sulfoxide).

    abstract::Under the right conditions, some biological systems can maintain high viability after being frozen and thawed, but many others (e.g., organs and many mammalian cells) cannot. To increase the rates of post-thaw viability and widen the library of living cells and tissues that can be stored frozen, an improved understand...


    pub_type: 杂志文章


    authors: Burkey AA,Hillsley A,Harris DT,Baltzegar JR,Zhang DY,Sprague WW,Rosales AM,Lynd NA

    更新日期:2020-08-10 00:00:00

  • Elastin-like Polypeptide (ELP) Charge Influences Self-Assembly of ELP-mCherry Fusion Proteins.

    abstract::Self-assembly of protein-polymer bioconjugates presents an elegant strategy for controlling nanostructure and orientation of globular proteins in functional materials. Recent work has shown that genetic fusion of globular protein mCherry to an elastin-like polypeptide (ELP) yields similar self-assembly behavior to the...


    pub_type: 杂志文章


    authors: Mills CE,Michaud Z,Olsen BD

    更新日期:2018-07-09 00:00:00

  • Poly(ethylene glycol)-poly(ester-carbonate) block copolymers carrying PEG-peptidyl-doxorubicin pendant side chains: synthesis and evaluation as anticancer conjugates.

    abstract::Water soluble polymer anticancer conjugates can improve the pharmacokinetics of covalently bound drugs by limiting cellular uptake to the endocytic route, thus prolonging plasma circulation time and consequently facilitating tumor targeting by the enhanced permeability and retention (EPR) effect. Many of the first gen...


    pub_type: 杂志文章


    authors: Andersson L,Davies J,Duncan R,Ferruti P,Ford J,Kneller S,Mendichi R,Pasut G,Schiavon O,Summerford C,Tirk A,Veronese FM,Vincenzi V,Wu G

    更新日期:2005-03-01 00:00:00

  • Photocurable Hyperbranched Polymer Medical Glue for Water-Resistant Bonding.

    abstract::Medical glue represents a suitable tool to stop wound bleeding, seal wounds, bond tissues, or implant materials. However, the development of a medical glue for durable underwater bonding remains a challenge. Herein, we developed a hydrophobic hyperbranched polymer-based medical glue with water-resistant bonding abilit...


    pub_type: 杂志文章


    authors: Zhang Y,Li X,Zhu Q,Wei W,Liu X

    更新日期:2020-12-14 00:00:00

  • Suppressing surface reconstruction of superhydrophobic PDMS using a superhydrophilic zwitterionic polymer.

    abstract::Poly(dimethyl siloxane) (PDMS) is extensively used for biomedical applications due to its low cost, ease of fabrication, high durability and flexibility, oxygen permeability, and self-healing properties. PDMS, however, has some significant drawbacks. PDMS endures unacceptably high levels of nonspecific protein fouling...


    pub_type: 杂志文章


    authors: Keefe AJ,Brault ND,Jiang S

    更新日期:2012-05-14 00:00:00

  • Injectable Shape-Holding Collagen Hydrogel for Cell Encapsulation and Delivery Cross-linked Using Thiol-Michael Addition Click Reaction.

    abstract::Injectable hydrogels based on extracellular matrix-derived polymers show much promise in the field of tissue engineering and regenerative medicine. However, the hydrogels reported to date have at least one characteristic that limits their potential for clinical use, such as excessive swelling, complicated and potentia...


    pub_type: 杂志文章


    authors: Pupkaite J,Rosenquist J,Hilborn J,Samanta A

    更新日期:2019-09-09 00:00:00

  • Studies on the mechanism of interaction of a bioresponsive endosomolytic polyamidoamine with interfaces. 1. Micelles as model surfaces.

    abstract::Polymers are appealing as pH-responsive elements of multicomponent systems designed to promote cytosolic delivery of macromolecular drugs (including proteins and genes), but so far the delivery efficiency achieved has been relatively modest. Therefore, the aim of this study was to apply several physicochemical techniq...


    pub_type: 杂志文章


    authors: Griffiths PC,Khayat Z,Tse S,Heenan RK,King SM,Duncan R

    更新日期:2007-03-01 00:00:00

  • Double-Network Hydrogels Including Enzymatically Crosslinked Poly-(2-alkyl-2-oxazoline)s for 3D Bioprinting of Cartilage-Engineering Constructs.

    abstract::Double-network (DN) hydrogels are fabricated from poly(2-ethyl-2-oxazoline) (PEOXA)-peptide conjugates, which can be enzymatically crosslinked in the presence of Sortase A (SA), and physical networks of alginate (Alg), yielding matrices with improved mechanical properties with respect to the corresponding PEOXA and Al...


    pub_type: 杂志文章


    authors: Trachsel L,Johnbosco C,Lang T,Benetti EM,Zenobi-Wong M

    更新日期:2019-12-09 00:00:00

  • Report on the use of poly(organophosphazenes) for the design of stimuli-responsive vesicles.

    abstract::A novel family of amphiphilic temperature- and pH-sensitive poly(organophosphazenes) with varying ratios of ethylene oxide, alkyl chains and free acid units was synthesized by living cationic polymerization. Depending on their composition, these poly(organophosphazenes) exhibited lower critical solution temperatures r...


    pub_type: 杂志文章


    authors: Couffin-Hoarau AC,Leroux JC

    更新日期:2004-11-01 00:00:00

  • High internal phase emulsion templating with self-emulsifying and thermoresponsive chitosan-graft-PNIPAM-graft-oligoproline.

    abstract::High internal phase emulsion (HIPE)-templating is an attractive method of producing high porosity polymer foams with tailored pore structure, pore size and porosity. However, this method typically requires the use of large amounts of surfactants to stabilize the immiscible liquid phases, and polymerizable monomers/cro...


    pub_type: 杂志文章


    authors: Oh BH,Bismarck A,Chan-Park MB

    更新日期:2014-05-12 00:00:00

  • A platelet-mimetic paradigm for metastasis-targeted nanomedicine platforms.

    abstract::There is compelling evidence that, beyond their traditional role in hemostasis and thrombosis, platelets play a significant role in mediating hematologic mechanisms of tumor metastasis by directly and indirectly interacting with pro-metastatic cancer cells. With this rationale, we hypothesized that platelets can be an...


    pub_type: 杂志文章


    authors: Modery-Pawlowski CL,Master AM,Pan V,Howard GP,Sen Gupta A

    更新日期:2013-03-11 00:00:00

  • Influence of enzymatic specificity on the behavior of ephemeral gels.

    abstract::Ephemeral gels, called Enzgels, successively undergo sol-gel and then gel-sol transition under the action of two antagonistic enzymes, transglutaminase and protease. Molecular and macroscopic properties of Enzgels are directly dependent on the enzymatic activities and their ratios. This work studies the characteristic...


    pub_type: 杂志文章


    authors: Picard J,Giraudier S,Larreta-Garde V

    更新日期:2008-01-01 00:00:00