Conserved C-termini of Spidroins are secreted by the major ampullate glands and retained in the silk thread.

Abstract:

:The C-termini of Spidroins produced in the major and minor ampullate glands of spiders are highly conserved. Despite this conservation, no corresponding peptides have been identified in the spinning dopes or the silk filaments so far. To prove their presence or absence, polyclonal antibodies derived against fusion proteins containing the conserved C-terminal regions of both Spidroin 1 and 2 from the spider Nephila clavipes were generated. The antibodies reacted with high molecular weight polypeptides of the corresponding gland extracts and solubilized major ampullate filament and in addition to filament cross-sections. This demonstrates the existence of C-terminal specific peptides in the spinning dope and the mature Spidroins. Both the fusion proteins as well as the proteins contained within the gland lumen showed a reduction in their size under reducing conditions indicating the presence of disulfide bonds. Their high conservation and the biochemical data suggest crucial roles the C-termini play in the formation and/or structure of the corresponding silk filaments.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Sponner A,Unger E,Grosse F,Weisshart K

doi

10.1021/bm034378b

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

840-5

issue

3

eissn

1525-7797

issn

1526-4602

journal_volume

5

pub_type

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