Abstract:
:The C-termini of Spidroins produced in the major and minor ampullate glands of spiders are highly conserved. Despite this conservation, no corresponding peptides have been identified in the spinning dopes or the silk filaments so far. To prove their presence or absence, polyclonal antibodies derived against fusion proteins containing the conserved C-terminal regions of both Spidroin 1 and 2 from the spider Nephila clavipes were generated. The antibodies reacted with high molecular weight polypeptides of the corresponding gland extracts and solubilized major ampullate filament and in addition to filament cross-sections. This demonstrates the existence of C-terminal specific peptides in the spinning dope and the mature Spidroins. Both the fusion proteins as well as the proteins contained within the gland lumen showed a reduction in their size under reducing conditions indicating the presence of disulfide bonds. Their high conservation and the biochemical data suggest crucial roles the C-termini play in the formation and/or structure of the corresponding silk filaments.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Sponner A,Unger E,Grosse F,Weisshart Kdoi
10.1021/bm034378bkeywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
840-5issue
3eissn
1525-7797issn
1526-4602journal_volume
5pub_type
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