Abstract:
:Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for solid tissues, there are many tissues and organs that are not amenable to such an approach. In this review I will focus on the development of electroporation protocols for two such tissues: the vasculature and the lung. Several methods for in vivo electroporation of the vasculature have been developed in recent years that deliver DNA to vessel segments from either the inside or outside of the vessel. The advantages and disadvantages of each are discussed, as are the applications for which they have been used. In more recent work, our laboratory has developed a novel method to deliver genes to the rodent lung that results in high level, uniform, gene expression throughout all cell types of the lung. Most importantly, this technique is safe, and causes no inflammatory response or alterations in normal physiology of the organs. Taken together, these studies demonstrate the utility of electroporation for gene transfer to non injectible tissues.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Dean DAdoi
10.1089/104454903322625000keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
797-806issue
12eissn
1044-5498issn
1557-7430journal_volume
22pub_type
杂志文章,评审abstract::We evaluated the effects of administration of 1,25-dihydroxyvitamin D (1,25(OH)2D) during pregnancy on relieving adverse outcomes of preeclampsia and the pathologic and biochemical changes in reduction in uteroplacental perfusion (RUPP) model of rats. On day 1, 7, and 14 of pregnancy, rats in pregnant RUPP plus 1,25(O...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2885
更新日期:2016-02-01 00:00:00
abstract::In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/1044549041474805
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2884
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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doi:10.1089/dna.2007.0694
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4469
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journal_title:DNA and cell biology
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doi:10.1089/dna.2011.1333
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doi:10.1089/dna.2009.0921
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2019-02-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignancy with relatively high incidence and poor prognosis. RNA-binding proteins (RBPs) were reported to be dysregulated in multiple cancers and were closely associated with tumor initiation and progression. However, an integrated analysis of the roles of RBPs in HN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5641
更新日期:2020-10-01 00:00:00
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journal_title:DNA and cell biology
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doi:10.1089/dna.1997.16.769
更新日期:1997-06-01 00:00:00
abstract::Culture of CHO.K1, a Chinese hamster ovary cell line, requires no particular care about the glucocorticoid level in media. Cell growth of CHO.K1 is little affected by dexamethasone at concentrations up to 3 microM. A clone of CHO.K1 stably transfected with an expression vector displayed a favored growth in dexamethaso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.125
更新日期:1996-02-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5574
更新日期:2020-09-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.59
更新日期:1994-01-01 00:00:00
abstract::The epidermal growth factor (EGF) pathway stimulates proliferation and differentiation of epidermal and epithelial tissues, and plays an important role in tumorigenesis. The association between EGF polymorphisms and cancer risk is controversial; thus, we performed this meta-analysis. Overall, 41 case-control studies w...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
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journal_title:DNA and cell biology
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pub_type: 杂志文章
doi:10.1089/dna.2019.4896
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更新日期:2012-03-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490252810311
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.9
更新日期:1997-01-01 00:00:00
abstract::Among environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is one of the most potent tumor promoters and teratogens known. The molecular mechanisms responsible for the biological activity of TCDD, however, remain largely unknown. In this report, we show that the first observable effects of TCDD...
journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
abstract::The CAT gene is widely used as a reporter in eukaryotic systems because of the efficient translation of its mRNA. We report here that a sequence occurring in the CAT mRNA at +15 nucleotides from CAT AUG is essential for translation. This sequence includes a stem-loop structure, which, however, exhibits a calculated st...
journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
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journal_title:DNA and cell biology
pub_type: 杂志文章,评审
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abstract::G0S8 is a member of a set of putative G0/G1 switch regulatory genes (G0S genes) selected by screening cDNA libraries prepared from blood mononuclear cells cultured for 2 hr with lectin and cycloheximide. Comparison of a full-length cDNA sequence with the corresponding genomic sequence reveals an open reading frame of ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.125
更新日期:1994-02-01 00:00:00