Abstract:
:Culture of CHO.K1, a Chinese hamster ovary cell line, requires no particular care about the glucocorticoid level in media. Cell growth of CHO.K1 is little affected by dexamethasone at concentrations up to 3 microM. A clone of CHO.K1 stably transfected with an expression vector displayed a favored growth in dexamethasone-containing media. Ironically, dexamethasone was used to trigger the expression of antisense PCNA from the expression vector to impede the cell growth. Proliferating cell nuclear antigen (PCNA), an auxiliary factor of DNA polymerase delta, is required for cell proliferation. The stable cell clone, designated as B11 had a retarded growth rate as compared to its parental cell. However, the B11 cell growth rate and the cell cycle progression were increased by dexamethasone. The glucocorticoid produced no similar effect on the parental cell or other stable transfectants of the same plasmid. Thus, the stimulatory effect of dexamethasone on B11 has little connection with the expression of antisense PCNA and possibly involves a relevant gene in the B11 genome that was mutated due to the random plasmid insertion. A preliminary effort in identifying the targeted gene was made by using plasmid rescue method, and two plasmids were obtained. The rescued DNA of both plasmids specifically hybridized genomic DNA of the parental cells, and one of these plasmids detected a cellular transcript that was absent in B11 cells, suggesting its potential for further investigation.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Chen GS,Lee CH,Lee YC,Chi CW,Liu YCdoi
10.1089/dna.1996.15.125subject
Has Abstractpub_date
1996-02-01 00:00:00pages
125-32issue
2eissn
1044-5498issn
1557-7430journal_volume
15pub_type
杂志文章abstract::Administration of endotoxin (LPS) in humans results in profound physiological responses, including activation of peripheral blood mononuclear cells and the release of inflammatory factors. The time course of the response of selected inflammatory proteins was examined in healthy subjects (n = 6) administered a single i...
journal_title:DNA and cell biology
pub_type: 临床试验,杂志文章
doi:10.1089/dna.2005.24.410
更新日期:2005-07-01 00:00:00
abstract::A human growth hormone expression construct was delivered intracellularly into the abdominal skin of mice by particle bombardment-mediated gene transfer. Using this technology, the in vivo delivery of antigen-encoding expression vectors affixed to gold microprojectiles results in de novo antigen production in target s...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.791
更新日期:1993-11-01 00:00:00
abstract::Gallbladder carcinoma (GBC) is a leading cause of cancer deaths in north India. Evidence has highlighted the role of abnormal DNA methylation patterns on inappropriate gene expression in development and progression of various cancers. 5,10-Methylenetetrahydrofolate reductase (MTHFR) plays a major role in provision of ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0679
更新日期:2008-03-01 00:00:00
abstract::Transgenic mice expressing interleukin-12 (IL-12) under the glial fibrillary acidic protein (GFAP) promoter were evaluated for their sensitivity to herpes simplex virus type 1 (HSV-1) infection of the cornea. There was a modest but significant decrease in the infiltration of mononuclear cells in the cornea of the GFAP...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490260099764
更新日期:2002-05-01 00:00:00
abstract::Voltage-gated potassium channels play important functional roles in the development and maintenance of human lymphocyte functions. One such channel, known as the type n channel, has been well defined in human T cells and exhibits unique functional properties that distinguish it from other species of potassium channels...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.163
更新日期:1992-03-01 00:00:00
abstract::Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA (miRNA) expression levels or processing and contribute to susceptibility to a wide range of diseases. We investigated the correlation between four SNPs (rs11614913, rs3746444, rs2910164, and rs229283) in pre-miRNAs and the risk of asthma in 220 as...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1164
更新日期:2011-11-01 00:00:00
abstract::Among environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is one of the most potent tumor promoters and teratogens known. The molecular mechanisms responsible for the biological activity of TCDD, however, remain largely unknown. In this report, we show that the first observable effects of TCDD...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.269
更新日期:1992-05-01 00:00:00
abstract::Sequence analysis of the 5' end of the thrombomodulin (TM) gene has identified a difference in two strains of laboratory mice, BALB/cBD (maintained at the Oak Ridge National Laboratory) and the strain used for the sequence listed in the GenBank data base. An intracisternal A-particle (IAP) provirus was present in the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.311
更新日期:1993-05-01 00:00:00
abstract::Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5136
更新日期:2020-03-01 00:00:00
abstract::Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.301
更新日期:1998-03-01 00:00:00
abstract::To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4282
更新日期:2019-02-01 00:00:00
abstract::Sufficient levels of gene expression are required for effective gene therapy. One of the major obstacles in gene therapy is the low transgene expression obtained from currently available vector systems. To address this issue, we employed a transcriptional amplifier strategy in a single construct to enhance transgene e...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.595
更新日期:2005-09-01 00:00:00
abstract::The application of endogenous regeneration in regenerative medicine is based on the concept of inducing regeneration of damaged or lost tissues from residual tissues in situ. Therefore, endogenous regeneration is also termed in vivo regeneration as opposed to mechanisms of ex vivo regeneration which are applied, for e...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322405464
更新日期:2003-09-01 00:00:00
abstract::The rat D2 dopamine receptor gene is transcribed from a TATA-less promoter that has an initiator-like sequence and several putative Sp1 binding sites. We previously reported that a negative modulator is located between nucleotides -116 and -76 (D2Neg-B) in this gene and that Sp1 as well as another unknown factor bind ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.471
更新日期:1998-05-01 00:00:00
abstract::Uterine corpus endometrial carcinoma (UCEC) is a common malignancy in the female reproductive system, associated with high morbidity and mortality. Despite the high prevalence of UCEC, molecular understanding of uterine endothelium tumorigenesis remains poorly understood. In this study, we reported that transcription ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4062
更新日期:2018-05-01 00:00:00
abstract::MicroRNAs (miRNAs) are an important class of small, noncoding, regulatory RNAs found to be involved in regulating a wide variety of important cellular processes by the sequence-specific inhibition of gene expression. Viruses have evolved a number of mechanisms to take advantage of the regulatory potential of this high...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2006.0559
更新日期:2007-04-01 00:00:00
abstract::Growth factors are important mediators of proliferation. Deregulation in growth factor mechanisms as well as in its receptors will contribute to cancer development. One of the most important is the epidermal growth factor (EGF), which is encoded by EGF gene. A functional polymorphism at position 61 (A/G) is associated...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0823
更新日期:2009-05-01 00:00:00
abstract::The long arm of chromosome 6 is frequently deleted in diverse human neoplasms. Our previous study showed a minimum deletion region between markers D6S1056 and D6S300 on chromosome 6q in primary prostate cancer (CaP). In this study, we further refined a 200-kb minimal region of deletion (6qTSG1) centered around D6S1013...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.597
更新日期:2006-11-01 00:00:00
abstract::The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900750058080
更新日期:2000-12-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::We have cloned and mapped the chromosomal location of three novel human genes encoding G protein-coupled receptors that we have named GPR6, GPR5, and GPR4. The entire coding region for each of these genes was contained on single exons. Gene GPR6 encoded a receptor that shared closest identity (71% in the transmembrane...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.25
更新日期:1995-01-01 00:00:00
abstract::Parkinson's disease (PD) is a serious neurodegenerative disorder that lacks effective therapeutic methods. In this research, expressions of PPARα, RXRα, and miR-21 were evaluated in PD patients and normal controls. To investigate the effects of miR-21, docosahexaenoic acid (DHA) and aspirin (ASA) on PD, as well as the...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3643
更新日期:2017-06-01 00:00:00
abstract::The aromatic hydrocarbon receptor (AHR) is a ligand-activated transcription factor that regulates the expression of several drug-metabolizing enzymes and has been implicated in immunosuppression, teratogenesis, cell-specific hyperplasia, and certain types of malignancies and toxicities. The mouse Ahr gene 5' proximal ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.811
更新日期:1998-09-01 00:00:00
abstract::DNA methylation plays an essential role in carcinogenesis. Promoter hypermethylation can result in transcriptional silencing of specific genes, such as tumor suppressors. Thus far, few reports have investigated the effect of curcumin, an active component of the perennial herb Curcuma longa, on DNA methylation. In the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2042
更新日期:2013-06-01 00:00:00
abstract::The CDC6 gene product from Saccharomyces cerevisiae is required for transition from late G1 to S phase of the cell cycle. We have investigated the subcellular localization of the CDC6 protein in yeast to explore where Cdc6p exerts its gene function (s). Using affinity-purified sera we localized Cdc6p to the cytoplasm ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.883
更新日期:1996-10-01 00:00:00
abstract::CONSTANS (CO)/CONSTANS-like (COL) genes that have been studied in annual model plants such as Arabidopsis thaliana and Oryza sativa play key roles in the photoperiodic flowering pathway. Moso bamboo is a perennial plant characterized by a long vegetative stage and flowers synchronously followed by widespread death. Ho...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4611
更新日期:2019-07-01 00:00:00
abstract::Protein-RNA complexation is essential in cell biological functions. Transfer RNAs are bound to aminoacyl-tRNA synthetases for the translation of the genetic code during protein synthesis, while ribonucleoproteins bind RNA in posttranscriptional regulation of gene expression. A recent report showed the interacton of hu...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.393
更新日期:2006-07-01 00:00:00
abstract::The centrosome is a unique organelle that functions as the microtubule organizing center in most animal cells. During cell division, the centrosomes form the poles of the bipolar mitotic spindle. In addition, the centrosomes are also needed for cytokinesis. Each mammalian somatic cell typically contains one centrosome...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/1044549041562276
更新日期:2004-08-01 00:00:00
abstract::Antibody-targeting therapy has drawn great interests to the hematologists and oncologists. 3A4, a novel antibody recognizing human CD45RA antigen, is a new target molecule for leukemias and holds a therapeutic potential for myeloid lineage leukemias. However, murine antibodies cannot be safely used in patients because...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4199
更新日期:2018-09-01 00:00:00
abstract::Several reports have suggested the importance of p73 polymorphisms in tumor behavior. We investigated the role of a p73 gene polymorphism in the susceptibility to cervical lesions in a southwestern European population. Peripheral blood samples were obtained from Radiotherapy and Gynaecology Departments of Portuguese I...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1294
更新日期:2012-02-01 00:00:00