Abstract:
:Sequence analysis of the 5' end of the thrombomodulin (TM) gene has identified a difference in two strains of laboratory mice, BALB/cBD (maintained at the Oak Ridge National Laboratory) and the strain used for the sequence listed in the GenBank data base. An intracisternal A-particle (IAP) provirus was present in the BALB/cBD sequence but absent from the GenBank sequence. Thus, there are two different 5' regions for the TM gene in mice. Southern blot and polymerase chain reaction analyses on other mouse strains demonstrated the presence of this proviral sequence in five out of ten strains tested. Northern blot analyses demonstrated that there were differences (up to three-fold) in the steady-state levels of TM mRNA among the strains. However, TM mRNA levels did not correlate directly with the presence of the IAP DNA upstream from the TM gene. This is in contrast to other loci (the interleukin-3 gene, c-mos, and the immunoglobulin Ck gene), in which the IAP element is known to affect levels of RNA transcription. While the IAP provirus does not appear to have an effect on TM transcription under normal conditions, it is possible that transcription levels altered by factors like thrombin and tumor necrosis factor are affected by the IAP provirus.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Ford VA,Kennel SJdoi
10.1089/dna.1993.12.311subject
Has Abstractpub_date
1993-05-01 00:00:00pages
311-8issue
4eissn
1044-5498issn
1557-7430journal_volume
12pub_type
杂志文章abstract::In the promoters of many immediate early genes, including c-fos, CArG DNA regulatory elements mediate basal constituitive expression and rapid and transient serum induction. CArG boxes also occur in the promoters of muscle-specific genes, including skeletal alpha-actin, where it confers muscle-specific expression. The...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.653
更新日期:1997-05-01 00:00:00
abstract::Chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing protein 6 (CMTM6) is a ubiquitously expressed protein, which plays a critical role in the stability of programmed death-ligand 1. However, the expression of CMTM6 in a variety of cancer pathological tissues is not clear. Therefore, 109 patients w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5564
更新日期:2020-10-21 00:00:00
abstract::Ulcerative colitis (UC) is a long-time inflammatory condition arising from aberrant immune activation in the colon and the rectum. Interleukin (IL)-35 plays critical roles in autoimmune disorders. In this study, we explored the pathways of IL-35 in affecting UC. First, peripheral blood mononuclear cells (PBMCs) from U...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3570
更新日期:2017-05-01 00:00:00
abstract::The polB gene encodes DNA polymerase II in Escherichia coli. The nucleotide sequence shows an open reading frame of 2,304 nucleotides coding for a protein of 88 kD. The protein initiation signal is preceded by a lexA box lying 2 nucleotides from the termination signal of araD, and begins with GUG 75 nucleotides after ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.631
更新日期:1990-11-01 00:00:00
abstract::Deer antlers are amazing natural appendages that grow faster than any other known mammalian bone. Antler growth occurs at the tip and is initially cartilage, which is later replaced by bone tissue. However, little is known regarding the precise role of cooperation between cell lineages and functional genes in regulati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3885
更新日期:2018-01-01 00:00:00
abstract::Most of the signal pathways involved in ultraviolet (UV)-induced skin carcinogenesis are thought to originate at plasma membrane receptors. However, UVA-induced signal transduction to downstream ribosomal protein S6 kinases, p70(S6K) and p90(RSK), is not well understood. In this report, we show that UVA stimulation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753438589
更新日期:2001-12-01 00:00:00
abstract::Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilti...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.6245
更新日期:2020-12-29 00:00:00
abstract::The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0586
更新日期:2007-08-01 00:00:00
abstract::Our aim was to evaluate in a cohort of 125 Italian patients with type 2 diabetes (T2D), who underwent neurological evaluation, the possible differences in the number of mitochondrial DNA copies (mtDNA) comparing positive and negative patients for cardiovascular autonomic neuropathy (CAN) or diabetic peripheral neuropa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5326
更新日期:2020-08-01 00:00:00
abstract::The incidence of premature ovarian failure (POF), a condition causing amenorrhea and hypergonadotropic hypoestrogenism in women before the age of 40, has been increasing in recent years. As an irreversible pathological change, improved treatment strategies for this disease are urgently needed. In this study, a type of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2032
更新日期:2013-12-01 00:00:00
abstract::Extracellular vesicles classified as exosomes, microvesicles, or apoptotic bodies based on size are shed from most cells under normal as well as pathological conditions. They are released into the surrounding milieu, including plasma, urine, saliva, and tissues. Exosomes are highly enriched in microRNAs (miRs), which ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2884
更新日期:2015-07-01 00:00:00
abstract::Leber congenital amaurosis (LCA) causes severe visual impairment and blindness very early in life. Mutant alleles of several genes acting in different pathways, of which all have critical roles for normal retinal function, were involved in LCA development. The purpose of this study was to use genome-wide genotyping to...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2554
更新日期:2014-12-01 00:00:00
abstract::Intracranial aneurysms (IAs) and potential IA rupture are one of the direct causes of permanent brain damage and mortality. Interestingly, the major risk factors of IA development, including hemodynamic stress, hypertension, smoking, and genetic predispositions, are closely associated with a proinflammatory immune sta...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.3105
更新日期:2016-02-01 00:00:00
abstract::Normal embryo development in oviparous (egg-laying) species requires the coordinated targeting to growing oocytes of nutrients and regulatory molecules such as retinol, the precursor of active retinoids. Serum retinol-binding protein (RBP) is the major carrier protein for retinol in the circulatory system of vertebrat...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.403
更新日期:1995-05-01 00:00:00
abstract::The aim of this study was to determine the role of polymorphic variants of apolipoprotein E (APOE) and peroxisome proliferator-activated receptor delta (PPARD) genes in the development of coronary heart disease (CHD), and the PPARD and APOE gene-gene interaction in a Turkish population. This study was carried out usin...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1464
更新日期:2012-05-01 00:00:00
abstract::To investigate the contribution of de novo variants to Tourette disorder (TD) probands in China. Whole-exome sequencing (WES) conducted on 15 child-parent trios (45 samples) detected 25 coding de novo variants, including 2 de novo Likely Gene Disrupting (LGD) variants and 6 Missense3 variants. The de novo LGD variants...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4746
更新日期:2020-01-01 00:00:00
abstract::Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.301
更新日期:1998-03-01 00:00:00
abstract::The application of endogenous regeneration in regenerative medicine is based on the concept of inducing regeneration of damaged or lost tissues from residual tissues in situ. Therefore, endogenous regeneration is also termed in vivo regeneration as opposed to mechanisms of ex vivo regeneration which are applied, for e...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322405464
更新日期:2003-09-01 00:00:00
abstract::CD36, a surface membrane glycoprotein, functions as a class B scavenger receptor that binds to several distinct ligands. Included among these is oxidized low-density lipoprotein (Ox-LDL), a major trigger of atherosclerotic lesions, and the levels of CD36 activity and Ox-LDL uptake may have an impact on coronary artery...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.302
更新日期:2006-05-01 00:00:00
abstract::Cytochrome P450c17 catalyzes the 17alpha-hydroxylase/17,20 lyase activity needed for sex steroid synthesis. We recently characterized the nuclear phosphoprotein SET as a novel transcriptional regulator that binds to the -447/-399 region of the rat P450c17 gene, along with the transcription factors COUP-TF II, NGF-IB, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753340604
更新日期:2001-10-01 00:00:00
abstract::The centrosome is a unique organelle that functions as the microtubule organizing center in most animal cells. During cell division, the centrosomes form the poles of the bipolar mitotic spindle. In addition, the centrosomes are also needed for cytokinesis. Each mammalian somatic cell typically contains one centrosome...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/1044549041562276
更新日期:2004-08-01 00:00:00
abstract::The murine 18A2/mts1 and its human homolog h-mts1 (S100A4), encoding a Ca2+-binding protein belonging to the S-100 family, are associated with high invasive and metastatic potentials of murine tumors, human tumor cell lines in vitro, and human tumors growing as xenografts. The nm23 is a putative metastasis-suppressor ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.335
更新日期:1998-04-01 00:00:00
abstract::We have analyzed the interleukin-1 beta (IL-1 beta) promoter region near the cap site. Specific DNA sequences required for lipopolysaccharide (LPS) induction within this region were identified using transfection of reporter plasmids that contained portions of the proximal IL-1 beta 5'-flanking sequence. An LPS-respons...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.561
更新日期:1994-06-01 00:00:00
abstract::Culture of CHO.K1, a Chinese hamster ovary cell line, requires no particular care about the glucocorticoid level in media. Cell growth of CHO.K1 is little affected by dexamethasone at concentrations up to 3 microM. A clone of CHO.K1 stably transfected with an expression vector displayed a favored growth in dexamethaso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.125
更新日期:1996-02-01 00:00:00
abstract::Transgenic mice expressing interleukin-12 (IL-12) under the glial fibrillary acidic protein (GFAP) promoter were evaluated for their sensitivity to herpes simplex virus type 1 (HSV-1) infection of the cornea. There was a modest but significant decrease in the infiltration of mononuclear cells in the cornea of the GFAP...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490260099764
更新日期:2002-05-01 00:00:00
abstract::We identified extracellular signal-regulated kinase 2 (erk2) from green mud crab, Scylla paramamosain, in this article. It was originally identified from an expressed sequence tag fragment from a normalized gonadal cDNA library. 5' Rapid amplification of cDNA end (RACE) technique was used to obtain the 5' untranslated...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1458
更新日期:2012-07-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::A decrease in gap junctional, intercellular communication (GJIC) has been associated with cells neoplastically transformed by a variety of factors. To investigate the role of the Ras oncogene product in gap junction function, a panel of murine C3H10T1/2 (10T1/2) fibroblasts was constructed in which the levels of ras g...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.443
更新日期:1996-06-01 00:00:00
abstract::Increasing evidence suggested that long noncoding RNAs (lncRNAs) variants may be involved in the progression of various cancers. However, the association of the lncRNAs polymorphisms with the risk for esophagogastric junction adenocarcinoma (EGJA) is still unknown. In this case-control study, we selected two cancer-re...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5368
更新日期:2020-05-01 00:00:00
abstract::Duchenne muscular dystrophy is the most common and severe form of progressive muscular dystrophy. Previous results showed an increased survival in double knockout mice (dko) when treated with adipose-derived CD146+ cells. In this study, we analyzed the effect of CD146+ cells compared to mesenchymal stem/stromal cells ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4158
更新日期:2018-09-01 00:00:00