Abstract:
:Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (Gpd2) encoding mtGPDH is reported. The mtGPDH gene spans 100 kb and consists of 17 exons. The use of alternate promoters was suggested by the presence of three different first exons and confirmed by transient expression for two of them. The first exons are expressed in a tissue-restricted manner. Exon 1a was found primarily in brain, exon 1b was used in all tissues examined, and exon 1c was detected predominantly in testis. Depending on the tissue, different transcript lengths were also observed: 5.9 kb (all tissues), 3.6 kb (skeletal muscle), and 2.5 kb (testis). The length isoforms are attributable to alternate splicing and polyadenylation site use. Very high mtGPDH mRNA levels were found in brown adipose tissue, 75 fold greater than in white adipose tissue. Thyroid hormone increased mtGPDH mRNA levels in liver and heart but not in brown adipose tissue, brain, or testis. This pattern corresponds to that of thyroid hormone-induced oxygen consumption and is consistent with a role for mtGPDH in thyroid hormone-induced thermogenesis. Both thyroid-responsive and nonresponsive tissues used promoter 1b, suggesting that tissue-specific factor(s) contribute to the tissue-restricted responsiveness to thyroid hormone.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Gong DW,Bi S,Weintraub BD,Reitman Mdoi
10.1089/dna.1998.17.301subject
Has Abstractpub_date
1998-03-01 00:00:00pages
301-9issue
3eissn
1044-5498issn
1557-7430journal_volume
17pub_type
杂志文章abstract::We have isolated and characterized a cDNA encoding a chicken beta homolog of c-erbA, or thyroid hormone receptor (TR). Chicken liver cDNA libraries were screened with a rat TR beta-1 cDNA probe, and several cDNA inserts were isolated and characterized. The sequence of one cDNA predicts a 369-amino-acid open reading fr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.211
更新日期:1991-04-01 00:00:00
abstract::The human spermidine synthase (EC 2.5.1.16) gene was isolated from a genomic library constructed with DNA obtained from a human immunoglobulin G (IgG) myeloma cell line. Subsequent sequence analyses revealed that the gene comprised of 5,818 nucleotides from the cap site to the last A of the putative polyadenylation si...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.467
更新日期:1991-07-01 00:00:00
abstract::Acetate and β-hydroxybutyrate (BHBA) are the predominant substrates for de novo fatty acid (FA) synthesis in mammary gland of dairy cow. To investigate the nutrigenomic role of acetate and BHBA in bovine mammary epithelial cells during milk fat production, RNA sequencing (RNA-seq) transcriptomic analysis was used to i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4783
更新日期:2020-03-01 00:00:00
abstract::The coupling of viral and arthropod host diversity, with evolving methods of virus discovery, has resulted in the identification and classification of a growing number of novel insect-specific viruses (ISVs) that appear to be evolutionarily related to many human pathogens but have either lost or have yet to gain the a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.4019
更新日期:2017-12-01 00:00:00
abstract::Most studies on the tissue distribution of actin isoform transcripts have been done in small mammals such as rat and mouse. We have begun a characterization of the actin gene family in a large mammal, the bovine. The alpha skeletal gene was isolated, and an isoform-specific probe to the 3' untranslated region of the t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.555
更新日期:1995-06-01 00:00:00
abstract::The family of G protein-coupled receptors (GPCRs) serves as the target for almost a third of currently marketed drugs, and provides the predominant mechanism through which extracellular factors transmit signals to the cell. The discovery of GPCRs with no known ligand has initiated a frenzy of research, with the aim of...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2005.24.54
更新日期:2005-01-01 00:00:00
abstract::A nomenclature system for the UDP glucuronosyltransferase superfamily is proposed, based on divergent evolution of the genes. A total of 26 distinct cDNAs in five mammalian species have been sequenced to date. Comparison of the deduced amino acid sequences leads to the definition of two families and a total of three s...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.1991.10.487
更新日期:1991-09-01 00:00:00
abstract::Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.729
更新日期:1993-10-01 00:00:00
abstract::Microsomal transfer protein (MTP), a lipid transfer protein localized in the endoplasmic reticulum of hepatocytes and enterocytes, plays an important role in the development of nonalcoholic fatty liver disease (NAFLD). Many existing studies have demonstrated that a common polymorphism (-493G>T, rs1800591 G>T) in the M...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.2238
更新日期:2014-06-01 00:00:00
abstract::Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites Ser* and Asn*...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.415
更新日期:1990-07-01 00:00:00
abstract::BRCA1-associated protein-1 (BAP1) is an important nuclear-localized deubiquitinating enzyme. Dysregulation of BAP1 has been reported in many types of cancers. However, there are few articles on the role of BAP1 in osteosarcoma (OS) and the molecular mechanisms of BAP1 in OS remain largely unknown. In this study, we ex...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3579
更新日期:2017-05-01 00:00:00
abstract::Palindromati, the massive host-edited synthetic palindromic contamination found in GenBank, is illustrated and exemplified. Millions of contaminated sequences with portions or tandems of such portions derived from the ZAP adaptor or related linkers are shown (1) by the 12-bp sequence reported elsewhere, exon Xb, 5' CC...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1339
更新日期:2012-02-01 00:00:00
abstract::Minimally modified low-density lipoprotein (MM-LDL) is regarded as a major risk factor for the development of atherosclerosis. In this report, we show that this lipoprotein complex can induce expression of an inflammatory protein, serum amyloid A (SAA), in monocyte/macrophage cells, a key cell type implicated in the p...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315637
更新日期:1999-01-01 00:00:00
abstract::Intestinal phospholipase A/lysophospholipase (IPAL) is an intestine-specific brush-border enzyme expressed during development and along the intestinal crypt-villus axis in a pattern similar to another well characterized brush-border enzyme, sucrase-isomaltase (SI). A tissue-specific DNase I hypersensitive site was ide...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1419
更新日期:1997-12-01 00:00:00
abstract::The sugars will eventually be exported transporters (SWEET) gene family is a glycoprotein gene family that can regulate the transport of sugar in plants and plays an important role in plant growth and development, as well as in response to environmental stress. In this study, Kentucky bluegrass (cv. Baron) seedlings w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5418
更新日期:2020-09-01 00:00:00
abstract::The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0586
更新日期:2007-08-01 00:00:00
abstract::Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314746
更新日期:1999-12-01 00:00:00
abstract::Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5136
更新日期:2020-03-01 00:00:00
abstract::A highly conserved aspartic acid residue present in the third membrane-spanning region of adrenergic and muscarinic receptors is directly involved in ligand binding. The five cloned somatostatin receptor subtypes also contain this residue at the same relative position. To test whether Asp-124 of the rat somatostatin r...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.939
更新日期:1995-11-01 00:00:00
abstract::In this study, we mined out hepatocellular carcinoma (HCC) driver genes from MEDLINE literatures by bioinformatics methods of pathway crosstalk and protein interaction network. Furthermore, the relationship between driver genes and their clinicopathological characteristics, as well as classification effectiveness was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4869
更新日期:2019-10-01 00:00:00
abstract::2-Arylbenzothiazoles are an important class of bicyclic privileged substructures present in various natural or synthetic compounds that have been shown to possess anticancer, antifungal, antibacterial, anti-inflammatory, and antiallergic activities. This study examined the antiproliferative properties of 2-(3,5-dihydr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1363
更新日期:2012-03-01 00:00:00
abstract::Protein-RNA complexation is essential in cell biological functions. Transfer RNAs are bound to aminoacyl-tRNA synthetases for the translation of the genetic code during protein synthesis, while ribonucleoproteins bind RNA in posttranscriptional regulation of gene expression. A recent report showed the interacton of hu...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.393
更新日期:2006-07-01 00:00:00
abstract::Our aim was to evaluate in a cohort of 125 Italian patients with type 2 diabetes (T2D), who underwent neurological evaluation, the possible differences in the number of mitochondrial DNA copies (mtDNA) comparing positive and negative patients for cardiovascular autonomic neuropathy (CAN) or diabetic peripheral neuropa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5326
更新日期:2020-08-01 00:00:00
abstract::The nuclear factor of κB (NFκB) family has been well known for its significant role in regulating the expression of numerous genes that control many biological processes. However, it is unclear whether NFκB could regulate milk synthesis. In this study, we identified NFκB1 as a critical regulator for milk synthesis in ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3610
更新日期:2017-04-01 00:00:00
abstract::Immune dysregulation has been implicated in myelodysplastic syndrome (MDS) pathogenesis. Refractory anemia with ringed sideroblasts (RARS) is a low-risk subtype of MDS. Our previous study has reported an abnormal γδ T cell receptor (TCR) repertoire in patients with intermediate or high-risk MDS. To characterize the st...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2925
更新日期:2015-09-01 00:00:00
abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilti...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.6245
更新日期:2020-12-29 00:00:00
abstract::The aim of this study was to determine the role of polymorphic variants of apolipoprotein E (APOE) and peroxisome proliferator-activated receptor delta (PPARD) genes in the development of coronary heart disease (CHD), and the PPARD and APOE gene-gene interaction in a Turkish population. This study was carried out usin...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1464
更新日期:2012-05-01 00:00:00
abstract::At supramolecular resolution, DNA synthesis begins at preferred replication origins in the chromosomes of metazoan cells. To characterize one of these origins in detail, the initiation of replication was examined in the HeLa c-myc origin. Polymerase chain reaction (PCR) amplification of size-fractionated nascent chrom...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.885
更新日期:1998-10-01 00:00:00
abstract::The amino acid sequences directing the nuclear transport of the RNA polymerase I transcription factor xUBF have been studied by a novel combination of in oocyte-coupled translation-nuclear transport and selective HCl extraction. Synthetic mRNA was used to direct the translation of labeled xUBF and its mutants in micro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.275
更新日期:1993-04-01 00:00:00