AZT incorporation into mitochondria: study in a human myeloid cell line.

abstract：:It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-de...

journal_title：DNA and cell biology

authors： Johansson VM,Oredsson SM,Alm K

更新日期：2008-09-01 00:00:00

abstract：:The search for new chemotherapeutics unaffected by efflux pumps would significantly increase life expectancy in patients with malignant cancers. In this study, butylcycloheptylprodigiosin and undecylprodigiosin were HPLC-purified and verified, using nuclear magnetic resonance spectroscopy. Cell cytotoxicity and transp...

journal_title：DNA and cell biology

authors： Mirzaei SA,Safari Kavishahi M,Keshavarz Z,Elahian F

更新日期：2018-06-01 00:00:00

abstract：:Gulf War Illness (GWI) affects about 25% of Persian Gulf veterans with a cluster of chronic symptoms, including immune dysfunction and neurological issues. Recent studies implicate gene expression changes in immune function to be associated with GWI. Since DNA methylation can regulate such changes in gene expression, ...

journal_title：DNA and cell biology

authors： Trivedi MS,Abreu MM,Sarria L,Rose N,Ahmed N,Beljanski V,Fletcher MA,Klimas NG,Nathanson L

更新日期：2019-06-01 00:00:00

abstract：:Genes involved in the production of nitric oxide (NO) have been suggested as genetic factors for migraine. It has been studied whether polymorphisms in the genes encoding for different types of NO synthase (NOS) could be involved in the liability to migraine; however, most studies yield negative results. The pentanucl...

journal_title：DNA and cell biology

authors： Jia S,Ni J,Chen S,Jiang Y,Dong W,Gao Y

更新日期：2011-02-01 00:00:00

abstract：:Human T cells express HLA class II molecules upon activation. The factors that regulate the induction of expression of these molecules are for the most part unknown. Here we report preliminary results indicating that tumor necrosis factor-alpha (TNF-alpha) regulates the induction of cell-surface HLA-DR, DO, and DP mol...

journal_title：DNA and cell biology

authors： Kamoun M,Zerva L,Sloan S,Zmijewski C,Monos D,Trinchieri G

更新日期：1992-04-01 00:00:00

abstract：:Endocytosis of the five rat somatostatin receptor subtypes (SSTR1-5) was investigated in transfected HEK cells by biochemical ligand binding assays and confocal microscopic analysis. Phenylarsine oxide-sensitive internalization of SSTR1-3 is dependent on SST-14 or SST-28, whereas only the octacosapeptide triggers this...

journal_title：DNA and cell biology

authors： Roth A,Kreienkamp HJ,Nehring RB,Roosterman D,Meyerhof W,Richter D

更新日期：1997-01-01 00:00:00

abstract：:Alzheimer's disease (AD) is a neurodegenerative disorder characterized by overproduction of beta-amyloid (Abeta), which is formed from amyloid precursor protein (APP), with the subsequent pathologic deposition of Abeta in regions of the brain important for memory and cognition. Recently, vaccination of murine models o...

journal_title：DNA and cell biology

authors： Dickey CA,Morgan DG,Kudchodkar S,Weiner DB,Bai Y,Cao C,Gordon MN,Ugen KE

更新日期：2001-11-01 00:00:00

abstract：:Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...

journal_title：DNA and cell biology

authors： Gao C,Yang Y,Zhang Y,Qian M,Yang J

更新日期：2020-03-01 00:00:00

abstract：:Global antibiotic resistance, driven by intensive antibiotic exposure/abuse, constitutes a serious challenge to all health care, particularly in an era when new antimicrobial development has slowed to a trickle. Recently, we published work demonstrating the discovery and partial mechanism of action of a novel bacteric...

journal_title：DNA and cell biology

authors： Hassett DJ,Meyer TJ

更新日期：2020-09-01 00:00:00

abstract：:Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...

journal_title：DNA and cell biology

authors： Huang H,Keohavong P

更新日期：1996-07-01 00:00:00

abstract：:The CAT gene is widely used as a reporter in eukaryotic systems because of the efficient translation of its mRNA. We report here that a sequence occurring in the CAT mRNA at +15 nucleotides from CAT AUG is essential for translation. This sequence includes a stem-loop structure, which, however, exhibits a calculated st...

journal_title：DNA and cell biology

authors： Ciullo M,Del Pozzo G,Autiero M,Guardiola J

更新日期：2000-01-01 00:00:00

abstract：:The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...

journal_title：DNA and cell biology

authors： Narayana A,Khodarev N,Walter S,Vaughan AT

更新日期：1998-07-01 00:00:00

abstract：:Butyrate is a naturally occurring product of colonic microbial fermentation of dietary carbohydrates that escape hydrolysis in the small intestine. Butyrate plays a significant role in the maintenance of colonic tissue homeostasis by regulating the expression of genes associated with the processes of proliferation, di...

journal_title：DNA and cell biology

authors： Daly K,Shirazi-Beechey SP

更新日期：2006-01-01 00:00:00

abstract：:Cardiomyocytes are notoriously difficult to transfect using standard techniques unless viral vectors such as recombinant adenoviruses are used. Generation of recombinant adenoviruses is, however, a complex and time-consuming procedure and not possible for every DNA construct. We therefore optimized DNA/polylysine/aden...

journal_title：DNA and cell biology

authors： Bauer S,Maier SK,Neyses L,Maass AH

更新日期：2005-06-01 00:00:00

abstract：:Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...

journal_title：DNA and cell biology

authors： Yu L,Miao H,Guo L

更新日期：2005-04-01 00:00:00

abstract：:We have cloned and sequenced the cDNA corresponding to the rat preproepidermal growth factor (ppEGF) mRNA. The cDNA contained 4,801 nucleotides, similar to that reported for the mouse (4,749 nucleotides) and the human mRNAs (4,871 nucleotides). The predicted protein sequence would contain 1,133 amino acids, smaller th...

journal_title：DNA and cell biology

authors： Saggi SJ,Safirstein R,Price PM

更新日期：1992-07-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a life-threatening disease that is often associated with chronic infection by hepatitis B and C viruses. Genetic polymorphisms have been reported to alter the risk for HCC development. Genetic studies based on the haplotype have an increased power for detecting disease associations co...

journal_title：DNA and cell biology

authors： Kiran M,Chawla YK,Jain M,Kaur J

更新日期：2009-11-01 00:00:00

abstract：:We have devised a method to determine gene structure that utilizes the known gene structure of homologous proteins and the polymerase chain reaction (PCR). Because homologous proteins have evolved from a common ancestral gene, it is possible to design primers corresponding to the adjacent exon sequences in the protein...

journal_title：DNA and cell biology

authors： Kunapuli SP,Stark P,Rick L,Colman RW

更新日期：1995-04-01 00:00:00

abstract：:Methionine is the limiting amino acid for milk protein synthesis in dairy cows. The effect of methionine availability on milk protein synthesis is dependent on its active transport into cells through amino acid transporters. L-type amino acid transporter 1 (LAT1), which induces the transport of neutral amino acids, is...

journal_title：DNA and cell biology

authors： Duan X,Lin Y,Lv H,Yang Y,Jiao H,Hou X

更新日期：2017-12-01 00:00:00

abstract：:Human monoclonal antibodies (HuMAbs) demonstrate great potential for passive immunotherapy against HIV-1. The gp41 transmembrane envelope glycoprotein of HIV has an important role in the pathogenicity of AIDS and importantly displays considerably less hypervariability than the gp120 surface envelope HIV glycoprotein, ...

journal_title：DNA and cell biology

authors： Cao C,Bai Y,Holloway MJ,Edgeworth RL,Jackson EA Jr,Cotropia J,Ugen KE

更新日期：2004-12-01 00:00:00

abstract：:A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG) has been known to possess antiproliferative properties. In this study, we investigated the anticancer effects of EGCG in human papillomavirus (HPV)-16 associated cervical cancer cell line, CaSki cells. The growth inhibitory mechanism(s) and regulation o...

journal_title：DNA and cell biology

authors： Ahn WS,Huh SW,Bae SM,Lee IP,Lee JM,Namkoong SE,Kim CK,Sin JI

更新日期：2003-03-01 00:00:00

abstract：:The pathogenic strains of Vibrio cholerae that cause acute enteric infections in humans are derived from environmental nonpathogenic strains. To track the evolution of pathogenic V. cholerae and identify potential precursors of new pathogenic strains, we analyzed 324 environmental or clinical V. cholerae isolates for ...

journal_title：DNA and cell biology

authors： Rahman MH,Biswas K,Hossain MA,Sack RB,Mekalanos JJ,Faruque SM

更新日期：2008-07-01 00:00:00

abstract：:In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...

journal_title：DNA and cell biology

authors： Watanabe Y,Hamaguchi-Tsuru E,Morimoto N,Nishio Y,Yagyu K,Konishi Y,Tominaga M,Miyazaki J,Furuya M,Tominaga A

更新日期：2004-07-01 00:00:00

abstract：:The long arm of chromosome 6 is frequently deleted in diverse human neoplasms. Our previous study showed a minimum deletion region between markers D6S1056 and D6S300 on chromosome 6q in primary prostate cancer (CaP). In this study, we further refined a 200-kb minimal region of deletion (6qTSG1) centered around D6S1013...

journal_title：DNA and cell biology

authors： Sun M,Srikantan V,Ma L,Li J,Zhang W,Petrovics G,Makarem M,Strovel JW,Horrigan SG,Augustus M,Sesterhenn IA,Moul JW,Chandrasekharappa S,Zou Z,Srivastava S

更新日期：2006-11-01 00:00:00

abstract：:The upstream transcription control region of the rat alpha-fetoprotein (AFP) gene was analyzed using transient expression of CAT genes in HepG2 cells which express the gene; H4C3 cells which repress the AFP gene but express the albumin gene; and four nonexpressing cell lines. Deletion analysis based on the DNA sequenc...

journal_title：DNA and cell biology

authors： Wen P,Groupp ER,Buzard G,Crawford N,Locker J

更新日期：1991-09-01 00:00:00

abstract：:By using the methodology of both wet and dry biology (i.e., RT-PCR and cycle sequencing, and biocomputational technology, respectively) and the data obtained through the Genome Projects, we have cloned Xenopus laevis SOD2 (MnSOD) cDNA and determined its nucleotide sequence. These data and the deduced protein primary s...

journal_title：DNA and cell biology

authors： Purrello M,Di Pietro C,Ragusa M,Pulvirenti A,Giugno R,Di Pietro V,Emmanuele G,Travali S,Scalia M,Shasha D,Ferro A

更新日期：2005-02-01 00:00:00

abstract：:Mutations in the mitochondrial genome (mtDNA) are associated with different types of cancer, specifically colorectal cancer (CRC). However, few studies have been performed on precancerous lesions, such as ulcerative colitis (UC) lesions and adenomatous polyps (AP). The aim of this study was to identify mtDNA mutations...

journal_title：DNA and cell biology

authors： Kassem AM,El-Guendy N,Tantawy M,Abdelhady H,El-Ghor A,Abdel Wahab AH

更新日期：2011-11-01 00:00:00

abstract：:Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA ...

journal_title：DNA and cell biology

authors： Heller R,Schultz J,Lucas ML,Jaroszeski MJ,Heller LC,Gilbert RA,Moelling K,Nicolau C

更新日期：2001-01-01 00:00:00

abstract：:Increasing evidence suggested that long noncoding RNAs (lncRNAs) variants may be involved in the progression of various cancers. However, the association of the lncRNAs polymorphisms with the risk for esophagogastric junction adenocarcinoma (EGJA) is still unknown. In this case-control study, we selected two cancer-re...

journal_title：DNA and cell biology

authors： Cao R,Chen Y,Wang J,Chen M,Chen S,Tang W

更新日期：2020-05-01 00:00:00

abstract：:The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate...

journal_title：DNA and cell biology

authors： Olsson AY,Persson AM,Valtonen-André C,Lundwall A

更新日期：2000-12-01 00:00:00