Effect of RNA interference on Gal alpha 1,3 Gal expression in PIEC cells.

Abstract:

:Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the elimination of Gal. Our study suggested that compared with the human alpha 1,2 fucosyltransferase (FT) gene and the porcine antisense alpha 1,3 galactosyltransferase gene, sequence-specific siRNA targeting Gal was capable of suppressing Gal expression markedly, and therefore, significantly inhibiting xenoreactivity and the complement activation with human serum in PIEC cells. We also demonstrated the concordant inhibitory effect of siRNA and the human FT gene on Gal and corresponding functions, which implied a practical significance of combined transgenic strategy. The successful application of vector-based dsRNA-GT may extend the list of available modalities in the abrogation of xenorejection in xenotransplantation.

journal_name

DNA Cell Biol

journal_title

DNA and cell biology

authors

Yu L,Miao H,Guo L

doi

10.1089/dna.2005.24.235

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

235-43

issue

4

eissn

1044-5498

issn

1557-7430

journal_volume

24

pub_type

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