Abstract:
:Homomeric and heteromeric interactions among cell-surface tyrosine kinase receptors belonging to the ErbB family lead to intracellular signaling cascades which are involved in cell activation, cytoskeletal interactions, and cellular transformation leading to neoplasia. Monoclonal antibodies which specifically bind to p185neu or epidermal growth factor receptor (EGFR), such as 7.16.4 and 225, respectively, can elicit tumor growth-inhibitory effects on transformed cells which overexpress either or both of these receptors. In order to better understand these receptor-receptor and receptor-antibody interactions and to gain insights that may be useful in the production and design of an antibody-based anticancer therapeutic, novel small recombinant 7.16.4 and 225 single-chain Fv fragments (scFv) were constructed, expressed, and characterized. We showed that these recombinant antibody fragments, which retain binding affinity, can be produced and purified from bacterial cell lysates. Our analyses further demonstrate that fusion of a 61 amino-acid dimerization domain with 7.16.4 and 225 scFv (7.16.4hth and 225hth) is sufficient to restore biological activity to these recombinant proteins.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Peterson NC,Greene MIdoi
10.1089/dna.1998.17.1031subject
Has Abstractpub_date
1998-12-01 00:00:00pages
1031-40issue
12eissn
1044-5498issn
1557-7430journal_volume
17pub_type
杂志文章abstract::Several reports have suggested the importance of p73 polymorphisms in tumor behavior. We investigated the role of a p73 gene polymorphism in the susceptibility to cervical lesions in a southwestern European population. Peripheral blood samples were obtained from Radiotherapy and Gynaecology Departments of Portuguese I...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1294
更新日期:2012-02-01 00:00:00
abstract::Ginsenoside, a natural triterpenoid saponin, exhibits immunomodulatory and anticancer activities. In the present study, we demonstrated that ginsenoside Rg1 induced secretion of cytokines, including interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and IL-1β, and chemokines such as IL-8 and IP-10 in a dose-depe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3923
更新日期:2017-12-01 00:00:00
abstract::Genes involved in the production of nitric oxide (NO) have been suggested as genetic factors for migraine. It has been studied whether polymorphisms in the genes encoding for different types of NO synthase (NOS) could be involved in the liability to migraine; however, most studies yield negative results. The pentanucl...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1102
更新日期:2011-02-01 00:00:00
abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903322216671
更新日期:2003-05-01 00:00:00
abstract::Colorectal cancer (CRC) patients with KRAS mutation are refractory and usually have poor prognosis. We aimed to identify the hub gene associated with KRAS mutant CRCs. Weighted gene coexpression network analysis (WGCNA) was used to calculate the key module and the hub genes in GSE39582. Combined with the protein-prote...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5574
更新日期:2020-09-01 00:00:00
abstract::BRCA1-associated protein-1 (BAP1) is an important nuclear-localized deubiquitinating enzyme. Dysregulation of BAP1 has been reported in many types of cancers. However, there are few articles on the role of BAP1 in osteosarcoma (OS) and the molecular mechanisms of BAP1 in OS remain largely unknown. In this study, we ex...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3579
更新日期:2017-05-01 00:00:00
abstract::Previously we cloned a polyspecific transporter from rat (rOCT1) that is expressed in renal proximal tubules and hepatocytes and mediates electrogenic uptake of organic cations with different molecular structures. Recently a homologous transporter from rat kidney (rOCT2) was cloned but not characterized in detail. We ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.871
更新日期:1997-07-01 00:00:00
abstract::Deer antlers are amazing natural appendages that grow faster than any other known mammalian bone. Antler growth occurs at the tip and is initially cartilage, which is later replaced by bone tissue. However, little is known regarding the precise role of cooperation between cell lineages and functional genes in regulati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3885
更新日期:2018-01-01 00:00:00
abstract::Voltage-gated potassium channels play important functional roles in the development and maintenance of human lymphocyte functions. One such channel, known as the type n channel, has been well defined in human T cells and exhibits unique functional properties that distinguish it from other species of potassium channels...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.163
更新日期:1992-03-01 00:00:00
abstract::Chlamydial infection of the lower genital tract usually spreads to the upper genital tract and is then responsible for more serious consequences, such as infertility, ectopic pregnancy, pelvic pain, and pelvic inflammatory disease. Genital infection with Chlamydia trachomatis and the resulting cytokine response largel...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1333
更新日期:2012-03-01 00:00:00
abstract::Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.301
更新日期:1998-03-01 00:00:00
abstract::A simple, efficient procedure for cross-linking two complementary oligonucleotides, which does not require chemical modification of either oligonucleotide, is described. One of the oligonucleotides is first converted to the 5'-phosphorothioate derivative with polynucleotide kinase. It is then incubated with its comple...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.71
更新日期:1990-01-01 00:00:00
abstract::Immune dysregulation has been implicated in myelodysplastic syndrome (MDS) pathogenesis. Refractory anemia with ringed sideroblasts (RARS) is a low-risk subtype of MDS. Our previous study has reported an abnormal γδ T cell receptor (TCR) repertoire in patients with intermediate or high-risk MDS. To characterize the st...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2925
更新日期:2015-09-01 00:00:00
abstract::Hepatic fibrosis is a reversible process involving plenty of transcription factors and pathways. Vitamin D receptor (VDR) as a member of ligand-induced transcription factors can interact with 9-cis retinoid X receptor (RXR) and VDR-interacting repressor (VDIR) to mediate transactivation or transrepression in the absen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3367
更新日期:2016-09-01 00:00:00
abstract::Duchenne muscular dystrophy is the most common and severe form of progressive muscular dystrophy. Previous results showed an increased survival in double knockout mice (dko) when treated with adipose-derived CD146+ cells. In this study, we analyzed the effect of CD146+ cells compared to mesenchymal stem/stromal cells ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4158
更新日期:2018-09-01 00:00:00
abstract::We have shown previously that rat hepatoma FTO-2B cells express two mRNAs, called F (fetal) and L (liver), from distinct promoters of the same gene coding for 6-phosphofructo-2-kinase (PFK-2). This enzyme catalyzes the synthesis of fructose 2,6-bisphosphate, an allosteric stimulator of glycolysis. We have now found th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1075
更新日期:1997-09-01 00:00:00
abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::It is widely believed that cellular senescence is a tumor suppressor mechanism; however, it has not been understood why it is advantageous for organisms to retain mutant cells is a postmitotic state rather than simply eliminating them by apoptosis. It has recently been proposed that the primary role of cellular senesc...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.69
更新日期:2006-02-01 00:00:00
abstract::To investigate the contribution of de novo variants to Tourette disorder (TD) probands in China. Whole-exome sequencing (WES) conducted on 15 child-parent trios (45 samples) detected 25 coding de novo variants, including 2 de novo Likely Gene Disrupting (LGD) variants and 6 Missense3 variants. The de novo LGD variants...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4746
更新日期:2020-01-01 00:00:00
abstract::We have analyzed transcription factor-mediated DNA supercoiling catalyzed by the Xenopus oocyte extract (S-150). Under conditions that inhibit endogenous supercoiling activity (2 mM EDTA), the 5S RNA specific transcription factor, TFIIIA, promotes a negative change in DNA linking number. The SV40 binding protein, T an...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.223
更新日期:1991-04-01 00:00:00
abstract::DNA methylation of CYP17 (steroid 17 alpha-hydroxylase) was studied in bovine adrenocortical cells, which lose the capacity to express this tissue-specific gene in culture by phenotypic switching. Restriction enzyme digestions, and sequencing of a lambda clone of a second CYP17 gene (CYP17A2), showed that there are at...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.385
更新日期:1992-06-01 00:00:00
abstract::Insulin-like growth factor I (IGF-I) plays a major role in development and metabolism. Currently, the cDNA-derived primary structure of IGF-I is known for some mammals and for chicken, frog, and salmon. Additionally, the organization of the human, rat, and chicken IGF-I genes has been established. The investigation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.729
更新日期:1993-10-01 00:00:00
abstract::Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320308942
更新日期:2002-08-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.589
更新日期:1996-07-01 00:00:00
abstract::We describe a new vector system that allows efficient expression of heterologous proteins in transformed mouse L fibroblasts. This is due to its persistence at high copy numbers, achieved by a 370-bp amplification promoting element (muNTS1) derived from the nontranscribed spacer of murine rDNA. Copy number determinati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.437
更新日期:1994-04-01 00:00:00
abstract::My 45 years of studying the molecular biology of adeno-associated virus are recounted. Additional activities as a mentor, department chair, and medical school administrator are described, as are my activities in the public sphere, which involved national issues related to science policy and medical education. ...
journal_title:DNA and cell biology
pub_type: 传,历史文章,杂志文章
doi:10.1089/dna.2013.2120
更新日期:2013-07-01 00:00:00
abstract::Polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) can be associated with phenotypic traits and be used as markers for disease diagnosis. Identification of these genetic variations within laboratory mice is crucial to improve our understanding of the genetic background of th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1353
更新日期:2012-03-01 00:00:00
abstract::In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/1044549041474805
更新日期:2004-07-01 00:00:00
abstract::Minimally modified low-density lipoprotein (MM-LDL) is regarded as a major risk factor for the development of atherosclerosis. In this report, we show that this lipoprotein complex can induce expression of an inflammatory protein, serum amyloid A (SAA), in monocyte/macrophage cells, a key cell type implicated in the p...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315637
更新日期:1999-01-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder characterized by overproduction of beta-amyloid (Abeta), which is formed from amyloid precursor protein (APP), with the subsequent pathologic deposition of Abeta in regions of the brain important for memory and cognition. Recently, vaccination of murine models o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490152717587
更新日期:2001-11-01 00:00:00