Abstract:
:Polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) can be associated with phenotypic traits and be used as markers for disease diagnosis. Identification of these genetic variations within laboratory mice is crucial to improve our understanding of the genetic background of the mice used for research. As part of a positional cloning project, we sequenced six genes (Mettl16, Evi2a, Psmd11, Cct6d, Rffl, and Ap2b1) within a 6.8-Mb domain of mmu chr 11 in the C57BL/6J and 129S6/SvEvTac inbred strains. Although 129S6/SvEvTac is widely used in the mouse community, there is very little current (or projected future) sequence information available for this strain. We identified 6 Indels and 21 novel SNPs and confirmed genotype information for 114 additional SNPs in these 6 genes. Mettl16 and Ap2b1 contained the largest numbers of variants between the C57BL/6J and 129S6/SvEvTac strains. In addition, we found five new SNPs between 129S6/SvEvTac and 129S1/SvImJ within the Ap2b1 locus. Although we did not detect differences between C57BL/6J and 129S6/SvEvTac within Evi2a, this locus contains a relatively high SNP density compared with the surrounding sequence. Our study highlights the genetic differences among three inbred mouse strains (C57BL/6J, 129S6/SvEvTac, and 129S1/SvImJ) and provides valuable sequence information that can be used to track alleles in genomics-based studies.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Lo CL,Shen F,Baumgarner K,Cramer MJ,Lossie ACdoi
10.1089/dna.2011.1353subject
Has Abstractpub_date
2012-03-01 00:00:00pages
402-14issue
3eissn
1044-5498issn
1557-7430journal_volume
31pub_type
杂志文章abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902753759726
更新日期:2002-04-01 00:00:00
abstract::Interferon gamma (IFN-γ) is one of the key cytokines that plays a major role against viral and intracellular bacterial infection. In addition to the IFN-γ gene, teleost fish possess a second copy known as IFN-γ-related (IFN-γrel) gene. This report describes structural and functional properties of IFN-γrel gene in the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2656
更新日期:2015-05-01 00:00:00
abstract::In our previous study, a kind of novel hybrid immunoglobulin (Ig)-binding proteins (IBPs) was obtained with the characteristic structure of alternately arranged Finegoldia magna (formerly Peptostreptococcus magnus) protein L (P. magnus protein L, PpL) B3 domain (B3) and Staphylococcal protein A (SpA) D domain (D). In ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0708
更新日期:2008-08-01 00:00:00
abstract::DNA repair is reduced in patients suffering from systemic lupus erythematosus (SLE), and it can induce the production of autoreactive antibodies due to the accumulation of DNA damage and nucleoprotein that produce immunogenic antigens. The accumulations of anti-Ku and DNA-PKcs antibodies, which are involved in nonhomo...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2465
更新日期:2015-05-01 00:00:00
abstract::This research aims to investigate the immune-associated gene signature from databases to improve the prognostic value in hepatocellular carcinoma (HCC) by multidimensional methods using various bioinformatic methods. Fifty-one immune-associated genes were mined out, which were associated with clinical characters throu...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.5099
更新日期:2020-04-01 00:00:00
abstract::Sestrin2 is involved in a different cellular response to stress conditions. However, the function of Sestrin2 in the cardiovascular system remains unknown. In the present study, we tested whether Sestrin2 has a beneficial effect on macrophage cell apoptosis induced by oxidized low-density lipoprotein (oxLDL). We found...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2627
更新日期:2015-04-01 00:00:00
abstract::CD36, a surface membrane glycoprotein, functions as a class B scavenger receptor that binds to several distinct ligands. Included among these is oxidized low-density lipoprotein (Ox-LDL), a major trigger of atherosclerotic lesions, and the levels of CD36 activity and Ox-LDL uptake may have an impact on coronary artery...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.302
更新日期:2006-05-01 00:00:00
abstract::Epigenetic modifications, especially alteration in DNA methylation, are increasingly being recognized as a key factor in the pathogenesis of complex disorders, including atherosclerosis. However, there are limited data on the epigenetic changes in the coronary artery disease (CAD) patients. In the present study we eva...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0694
更新日期:2008-07-01 00:00:00
abstract::A highly conserved aspartic acid residue present in the third membrane-spanning region of adrenergic and muscarinic receptors is directly involved in ligand binding. The five cloned somatostatin receptor subtypes also contain this residue at the same relative position. To test whether Asp-124 of the rat somatostatin r...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.939
更新日期:1995-11-01 00:00:00
abstract::Among environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is one of the most potent tumor promoters and teratogens known. The molecular mechanisms responsible for the biological activity of TCDD, however, remain largely unknown. In this report, we show that the first observable effects of TCDD...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.269
更新日期:1992-05-01 00:00:00
abstract::Ginsenoside, a natural triterpenoid saponin, exhibits immunomodulatory and anticancer activities. In the present study, we demonstrated that ginsenoside Rg1 induced secretion of cytokines, including interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and IL-1β, and chemokines such as IL-8 and IP-10 in a dose-depe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3923
更新日期:2017-12-01 00:00:00
abstract::FOXO3 is a member of the Forkhead box class O (FOXO) transcription factor family that modulates the transcription of target genes in a variety of cellular functions in lower animals to mammals. It has been implicated in the attenuation of insulin-signaling pathways, including having a potential role in lifespan contro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1193
更新日期:2011-07-01 00:00:00
abstract::Endogenous phytohormones auxin (indole-3-acetic acid [IAA]), abscisic acid (ABA), gibberellin (GA3), and brassinosteroid (BR) play a role in responses to drought stress in higher plants. Tea plant is one of the major economic corps worldwide. The tender shoots of tea plants are the main source for tea production. The ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4896
更新日期:2019-11-01 00:00:00
abstract::Sequence analysis of the 5' end of the thrombomodulin (TM) gene has identified a difference in two strains of laboratory mice, BALB/cBD (maintained at the Oak Ridge National Laboratory) and the strain used for the sequence listed in the GenBank data base. An intracisternal A-particle (IAP) provirus was present in the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.311
更新日期:1993-05-01 00:00:00
abstract::Mitochondrial dysfunction arises from an inadequate number of mitochondria, an inability to provide necessary substrates to mitochondria, or a dysfunction in their electron transport and a denosine triphosphate synthesis machinery. Occurrences of mitochondrial dysfunction are due to genetic or environmental changes in...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.5013
更新日期:2020-08-01 00:00:00
abstract::Mammalian cumulus-oocyte complexes (COCs) reach full developmental capability during folliculogenesis and oogenesis. It is well recognized that only gametes achieving MII stage after in vivo or in vitro maturation (IVM) are successfully fertilized by a single spermatozoon. Although the process of oocyte nuclear and/or...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3677
更新日期:2017-08-01 00:00:00
abstract::Endothelin-converting enzyme-1 (ECE1) plays a key role in the development of a subset of neural crest lineages such as cardiogenesis. Genetic variants of ECE1 C338A (rs213045) and T839G (rs213046) have been shown to alter ECE1 expression. This observation led us to hypothesize that two polymorphisms might influence th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2012.1626
更新日期:2012-08-01 00:00:00
abstract::The sugars will eventually be exported transporters (SWEET) gene family is a glycoprotein gene family that can regulate the transport of sugar in plants and plays an important role in plant growth and development, as well as in response to environmental stress. In this study, Kentucky bluegrass (cv. Baron) seedlings w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5418
更新日期:2020-09-01 00:00:00
abstract::Treatment with demethylating drugs can induce demethylation and reactivation of abnormally silenced tumor suppressor genes in cancer cells, but it can also induce potentially deleterious loss of methylation of repetitive elements. To enable the observation of unwanted drug effects related to loss of methylation of rep...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1537
更新日期:2012-10-01 00:00:00
abstract::We have isolated and characterized a cDNA encoding a chicken beta homolog of c-erbA, or thyroid hormone receptor (TR). Chicken liver cDNA libraries were screened with a rat TR beta-1 cDNA probe, and several cDNA inserts were isolated and characterized. The sequence of one cDNA predicts a 369-amino-acid open reading fr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.211
更新日期:1991-04-01 00:00:00
abstract::Chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing protein 6 (CMTM6) is a ubiquitously expressed protein, which plays a critical role in the stability of programmed death-ligand 1. However, the expression of CMTM6 in a variety of cancer pathological tissues is not clear. Therefore, 109 patients w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5564
更新日期:2020-10-21 00:00:00
abstract::Matrix metalloproteinase-7 (MMP-7) is a small secreted proteolytic enzyme with broad substrate specificity. Its expression is associated with tumor invasion, metastasis, and survival for a number of cancers. However, data from published studies with individually low statistical power are conflicting. Here, we performe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1416
更新日期:2012-05-01 00:00:00
abstract::Colorectal cancer (CRC) patients with KRAS mutation are refractory and usually have poor prognosis. We aimed to identify the hub gene associated with KRAS mutant CRCs. Weighted gene coexpression network analysis (WGCNA) was used to calculate the key module and the hub genes in GSE39582. Combined with the protein-prote...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5574
更新日期:2020-09-01 00:00:00
abstract::Gulf War Illness (GWI) affects about 25% of Persian Gulf veterans with a cluster of chronic symptoms, including immune dysfunction and neurological issues. Recent studies implicate gene expression changes in immune function to be associated with GWI. Since DNA methylation can regulate such changes in gene expression, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4469
更新日期:2019-06-01 00:00:00
abstract::Several reports have suggested the importance of p73 polymorphisms in tumor behavior. We investigated the role of a p73 gene polymorphism in the susceptibility to cervical lesions in a southwestern European population. Peripheral blood samples were obtained from Radiotherapy and Gynaecology Departments of Portuguese I...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1294
更新日期:2012-02-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.589
更新日期:1996-07-01 00:00:00
abstract::The activation of H-plk (Human-proviral linked Krüppel), a human Krüppel-related zinc finger gene in organs such as placenta, adrenal cortex, and testis, is probably due to insertion of an endogenous retrovirus, ERV3, upstream of the gene. Several differently spliced transcripts originate from this locus, e.g., a tran...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.27
更新日期:1998-01-01 00:00:00
abstract::Culture of CHO.K1, a Chinese hamster ovary cell line, requires no particular care about the glucocorticoid level in media. Cell growth of CHO.K1 is little affected by dexamethasone at concentrations up to 3 microM. A clone of CHO.K1 stably transfected with an expression vector displayed a favored growth in dexamethaso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.125
更新日期:1996-02-01 00:00:00
abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::Apicomplexa is a phylum within the kingdom Protista that contains some of the most significant threats to public health. One of the members of this phylum, Toxoplasma gondii, is amenable to molecular genetic analyses allowing for the identification of factors critical for colonization and disease. A pathway found to b...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2009.0853
更新日期:2009-06-01 00:00:00