Abstract:
:Epigenetic modifications, especially alteration in DNA methylation, are increasingly being recognized as a key factor in the pathogenesis of complex disorders, including atherosclerosis. However, there are limited data on the epigenetic changes in the coronary artery disease (CAD) patients. In the present study we evaluated the methylation status of genomic DNA from peripheral lymphocytes in a cohort of 287 individuals: 137 angiographically confirmed CAD patients and 150 controls. The differential susceptibility of genomic DNA to methylation-sensitive restriction enzymes was utilized to assess the methylation status of the genome. We observed that the genomic DNA methylation in CAD patients is significantly higher than in controls (p < 0.05). Since elevated homocysteine levels are known to be an independent risk factor for CAD and a key modulator of macromolecular methylation, we investigated the probable correlation between plasma homocysteine levels and global DNA methylation. We observed a significant positive correlation of global DNA methylation with plasma homocysteine levels in CAD patients (p = 0.001). Further, within a higher range of serum homocysteine levels (>/=12-50 muM), global DNA methylation was significantly higher in CAD patients than in controls. The alteration in genomic DNA methylation associated with cardiovascular disease per se appears to be further accentuated by higher homocysteine levels.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Sharma P,Kumar J,Garg G,Kumar A,Patowary A,Karthikeyan G,Ramakrishnan L,Brahmachari V,Sengupta Sdoi
10.1089/dna.2007.0694subject
Has Abstractpub_date
2008-07-01 00:00:00pages
357-65issue
7eissn
1044-5498issn
1557-7430journal_volume
27pub_type
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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doi:10.1089/dna.2010.1159
更新日期:2011-06-01 00:00:00
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journal_title:DNA and cell biology
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doi:10.1089/dna.2014.2627
更新日期:2015-04-01 00:00:00
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journal_title:DNA and cell biology
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更新日期:2020-03-01 00:00:00
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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abstract::Several reports have suggested the importance of p73 polymorphisms in tumor behavior. We investigated the role of a p73 gene polymorphism in the susceptibility to cervical lesions in a southwestern European population. Peripheral blood samples were obtained from Radiotherapy and Gynaecology Departments of Portuguese I...
journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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abstract::Regenerative medicine is a new interdisciplinary field in biomedical science, which aims at the repair or replacement of the defective tissue or organ by congenital defects, age, injury, or disease. Various cell-related techniques such as stem cell-based biotherapy are a hot topic in the current press, and stem cell r...
journal_title:DNA and cell biology
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abstract::A human growth hormone expression construct was delivered intracellularly into the abdominal skin of mice by particle bombardment-mediated gene transfer. Using this technology, the in vivo delivery of antigen-encoding expression vectors affixed to gold microprojectiles results in de novo antigen production in target s...
journal_title:DNA and cell biology
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abstract::Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...
journal_title:DNA and cell biology
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abstract::Haplotypes formed by polymorphisms (T-786C, rs2070744; a variable number of tandem repeats in intron 4, and Glu298Asp, rs1799983) of the eNOS gene were associated previously with gestational hypertension (GH) and preeclampsia (PE). However, no study has explored the Tag SNPs rs743506 and rs7830 in these disorders. The...
journal_title:DNA and cell biology
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abstract::ERGIC2 (formerly known as PTX1) is a gene identified by subtractive hybridization on the basis that it is expressed in normal human prostate, but not in prostate carcinoma. It is unrelated to the gene encoding pituitary homeobox protein (Ptx1 or Pitx1), which regulates pituitary hormone gene expression. Based on seque...
journal_title:DNA and cell biology
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更新日期:2014-02-01 00:00:00