Abstract:
:A subtractive cloning procedure was used to characterize the molecular changes involved in transformation of normal myoblasts to rhabdomyosarcoma (RMS) cells. Here we describe the cloning of DRAL, a novel LIM-domain protein expressed in primary myoblasts but down-regulated in the RMS cell line RD. DRAL is a LIM-only protein with five LIM domains whereby one LIM domain consists only of the second half of the consensus motif. Interestingly, down-regulation of DRAL was not confined to the RD RMS cells, but was a phenomenon extended to other RMS cell lines of both embryonal and alveolar subtype, and to some breast cancer cell lines. Analysis of the expression pattern in normal human tissues revealed that DRAL is expressed at high levels in the heart, suggesting an important function in the specification of the terminally differentiated phenotype of heart muscle cells. Immunofluorescence studies using an antibody directed against recombinant DRAL localized the protein predominantly in the nucleus of cultured cells. On the basis of these results, we conclude that down-regulation of DRAL correlates with the tumor phenotype of RMS cells.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Genini M,Schwalbe P,Scholl FA,Remppis A,Mattei MG,Schäfer BWdoi
10.1089/dna.1997.16.433subject
Has Abstractpub_date
1997-04-01 00:00:00pages
433-42issue
4eissn
1044-5498issn
1557-7430journal_volume
16pub_type
杂志文章abstract::RUNX2 is a member of the RUNX family of transcription factors, also containing the RUNX1 and RUNX3 proteins. These factors control the expression of genes essential for proper development in many cell lineages. RUNX2 plays a crucial role in the proliferation and differentiation of osteoblasts, required for bone format...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.721
更新日期:1992-12-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2089
更新日期:2014-03-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2015-11-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.579
更新日期:1992-10-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.125
更新日期:1995-02-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0855
更新日期:2009-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2019.5316
更新日期:2020-05-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章,评审
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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更新日期:2006-08-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00