Abstract:
:Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD polypeptide originally purified from media conditioned by interleukin-1 beta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the normal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK cells, 49F, we found that lipopolysaccharide (LPS), IL-1 beta, and tumor necrosis factor-alpha (TNF-alpha) each induce synthesis of CINC mRNA and CINC, although in qualitatively and quantitatively different patterns. Through deadenylation experiments and by probing with oligonucleotides, we discovered that the smaller of the two major CINC transcripts appears to arise from the larger as a result of poly(A) tail removal and/or 3' cleavage.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Dolecki GJ,Delarco JEdoi
10.1089/dna.1994.13.883subject
Has Abstractpub_date
1994-09-01 00:00:00pages
883-9issue
9eissn
1044-5498issn
1557-7430journal_volume
13pub_type
杂志文章abstract::Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5136
更新日期:2020-03-01 00:00:00
abstract::The incidence of premature ovarian failure (POF), a condition causing amenorrhea and hypergonadotropic hypoestrogenism in women before the age of 40, has been increasing in recent years. As an irreversible pathological change, improved treatment strategies for this disease are urgently needed. In this study, a type of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2032
更新日期:2013-12-01 00:00:00
abstract::The serotonin (5-hydroxytryptamine) transporter (5HTt) gene has been reported to be associated with suicidal behavior. In this study, we have investigated the 5' promoter region (i.e., 5HTt gene-linked polymorphic region [5-HTTLPR]) and a 17-base pair variable number of tandem repeats polymorphism in the 5HTt gene for...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1031
更新日期:2010-08-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.589
更新日期:1996-07-01 00:00:00
abstract::The G0S19 genes are members of the "small inducible" family of genes, which have similar exon-intron organizations and encode secreted proteins with similar dispositions of cysteine and proline residues. G0S19-1 mRNA is increased shortly after the addition of lectin or cycloheximide to cultured human blood mononuclear...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.589
更新日期:1990-10-01 00:00:00
abstract::The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900750058080
更新日期:2000-12-01 00:00:00
abstract::A yeast two-hybrid system was used to identify mutants of Max that exhibit an increased affinity for Myc. Truncated forms of the Max helix-loop-helix/leucine zipper motif (HLH/Zip) were first expressed in a two- hybrid system in which the bait protein was the HLH/Zip motif of Myc. Deletion of amino acids both amino-te...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.1277
更新日期:1997-11-01 00:00:00
abstract::Several studies have revealed that miR-205 plays important roles in the development of gynecological cancers and thus may serve as a potential prognostic biomarker, but the current conclusions remain controversial. Therefore, the goal of this study was to explore the prognostic significance and functional mechanisms o...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2019.5316
更新日期:2020-05-01 00:00:00
abstract::Mutations in the mitochondrial genome (mtDNA) are associated with different types of cancer, specifically colorectal cancer (CRC). However, few studies have been performed on precancerous lesions, such as ulcerative colitis (UC) lesions and adenomatous polyps (AP). The aim of this study was to identify mtDNA mutations...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1186
更新日期:2011-11-01 00:00:00
abstract::Mitochondrial dysfunction arises from an inadequate number of mitochondria, an inability to provide necessary substrates to mitochondria, or a dysfunction in their electron transport and a denosine triphosphate synthesis machinery. Occurrences of mitochondrial dysfunction are due to genetic or environmental changes in...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.5013
更新日期:2020-08-01 00:00:00
abstract::Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilti...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.6245
更新日期:2020-12-29 00:00:00
abstract::In this study, we isolated two cDNA molecules encoding putative glutamate receptor subunits, fGluR1 alpha and fGluR1 beta, from an Oreochromis sp. brain cDNA library by hybridizing with the glutamate receptor cDNA, fGluR2 beta, of the same fish. The deduced amino acid sequence of the fGluR1 alpha consists of 908 resid...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.717
更新日期:1996-09-01 00:00:00
abstract::Deer antlers are amazing natural appendages that grow faster than any other known mammalian bone. Antler growth occurs at the tip and is initially cartilage, which is later replaced by bone tissue. However, little is known regarding the precise role of cooperation between cell lineages and functional genes in regulati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3885
更新日期:2018-01-01 00:00:00
abstract::At supramolecular resolution, DNA synthesis begins at preferred replication origins in the chromosomes of metazoan cells. To characterize one of these origins in detail, the initiation of replication was examined in the HeLa c-myc origin. Polymerase chain reaction (PCR) amplification of size-fractionated nascent chrom...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.885
更新日期:1998-10-01 00:00:00
abstract::In the present study, the domain IIIs of all four dengue virus (DENV) serotypes were connected sequentially to construct the tandem domain III. The resulting DNA fragment was then cloned into pBAD/Topo ThioFusion plasmid. After induction, the Escherichia coli expression protein was purified and used to immunize BALB/c...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.0547
更新日期:2007-06-01 00:00:00
abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903322216671
更新日期:2003-05-01 00:00:00
abstract::The sex steroid hormone progesterone modulates the developmental and lactogenic activity of prolactin in the mammary gland. Regulation of the level of prolactin receptor (PRLR) provides one possible mechanism by which this may occur, prompting this investigation of the molecular mechanisms involved in progestin regula...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.721
更新日期:1992-12-01 00:00:00
abstract::Treatment with demethylating drugs can induce demethylation and reactivation of abnormally silenced tumor suppressor genes in cancer cells, but it can also induce potentially deleterious loss of methylation of repetitive elements. To enable the observation of unwanted drug effects related to loss of methylation of rep...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1537
更新日期:2012-10-01 00:00:00
abstract::We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.1063
更新日期:1996-12-01 00:00:00
abstract::The spike (S) protein, a main surface antigen of the SARS coronavirus (SARS-CoV), is considered to be one of the most important protective antigen candidates for targets for vaccine design against the virus. In this study, a secreted recombinant expression plasmid, pVAX-S1, encoding the partial S protein with a signal...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.516
更新日期:2005-08-01 00:00:00
abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.613
更新日期:1998-07-01 00:00:00
abstract::An increasing amount of evidence suggests that interleukin-18 (IL-18) plays a pivotal role in the pathophysiology of schizophrenia. However, association between single nucleotide polymorphism of IL-18 and the risk of schizophrenia has not been clarified. This study examined whether two promoter polymorphisms -137 G/C ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1221
更新日期:2011-11-01 00:00:00
abstract::Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.301
更新日期:1998-03-01 00:00:00
abstract::Sterol carrier protein2 (SCP2) is a 13.2-kD protein that is thought to be involved in the intracellular transport of cholesterol. Using synthetic oligonucleotides based on the protein sequence of SCP2, a clone (SP43) was isolated from a rat liver cDNA library. The DNA sequence revealed that the cDNA could encode a pol...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.159
更新日期:1990-04-01 00:00:00
abstract::The application of endogenous regeneration in regenerative medicine is based on the concept of inducing regeneration of damaged or lost tissues from residual tissues in situ. Therefore, endogenous regeneration is also termed in vivo regeneration as opposed to mechanisms of ex vivo regeneration which are applied, for e...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454903322405464
更新日期:2003-09-01 00:00:00
abstract::Recently, we demonstrated that the DAN gene product contains a growth- and/or a tumor-suppressive activity in vitro. In the present work, using a yeast two-hybrid system, we searched for cellular proteins that can associate with the DAN gene product. A cDNA clone, termed DA41, was initially isolated from an adult rat ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.985
更新日期:1997-08-01 00:00:00
abstract::We evaluated the effects of administration of 1,25-dihydroxyvitamin D (1,25(OH)2D) during pregnancy on relieving adverse outcomes of preeclampsia and the pathologic and biochemical changes in reduction in uteroplacental perfusion (RUPP) model of rats. On day 1, 7, and 14 of pregnancy, rats in pregnant RUPP plus 1,25(O...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2885
更新日期:2016-02-01 00:00:00
abstract::Thioredoxin peroxidase (TPx) is an antioxidant protein that limits the activity of reactive oxygen species (ROS). We cloned the cDNA encoding the mouse homolog of TPx from an E14.5 brain cDNA library and analyzed its distribution and function in murine tissues. Comparison of the amino acid sequence of mouse TPx with t...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.311
更新日期:1997-03-01 00:00:00
abstract::Leber congenital amaurosis (LCA) causes severe visual impairment and blindness very early in life. Mutant alleles of several genes acting in different pathways, of which all have critical roles for normal retinal function, were involved in LCA development. The purpose of this study was to use genome-wide genotyping to...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2554
更新日期:2014-12-01 00:00:00
abstract::Human monoclonal antibodies (HuMAbs) demonstrate great potential for passive immunotherapy against HIV-1. The gp41 transmembrane envelope glycoprotein of HIV has an important role in the pathogenicity of AIDS and importantly displays considerably less hypervariability than the gp120 surface envelope HIV glycoprotein, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2004.23.836
更新日期:2004-12-01 00:00:00