Abstract:
:We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear proteins that cooperate to stimulate transcription from the AFP and the thymidine kinase promoters in HepG2 hepatoma cells. Region 1, recently shown to be crucial for correct function of the enhancer in liver of transgenic mice, is recognized by two sets of transcription factors that bind to partly overlapping sites, 1a and 1b, in a noncooperative and nonexclusive manner. Site 1a contains a motif, AGGTCA, which is recognized by chicken ovalbumin upstream promoter transcription factors (COUP-TFs), but not by hepatocyte nuclear factor 4. Hepatocyte nuclear factor 3 (HNF3) and CCAAT/enhancer binding protein (C/EBP), which bind to regions 2 and 3, respectively, are likely responsible for the liver-specific E3 action. They play a key role by acting in synergy. The participation of nuclear receptors such as COUP-TFs, with C/EBP and HNF3, in the tight control of the distal AFP enhancer is a new, and perhaps key, step toward understanding the regulation and function of this enhancer, which may remain active throughout development.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Thomassin H,Bois-Joyeux B,Delille R,Ikonomova R,Danan JLdoi
10.1089/dna.1996.15.1063subject
Has Abstractpub_date
1996-12-01 00:00:00pages
1063-74issue
12eissn
1044-5498issn
1557-7430journal_volume
15pub_type
杂志文章abstract::Chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing protein 6 (CMTM6) is a ubiquitously expressed protein, which plays a critical role in the stability of programmed death-ligand 1. However, the expression of CMTM6 in a variety of cancer pathological tissues is not clear. Therefore, 109 patients w...
journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::2-Arylbenzothiazoles are an important class of bicyclic privileged substructures present in various natural or synthetic compounds that have been shown to possess anticancer, antifungal, antibacterial, anti-inflammatory, and antiallergic activities. This study examined the antiproliferative properties of 2-(3,5-dihydr...
journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::We previously showed that the human AKNA gene encodes an AT-hook transcription factor that regulates the expression of costimulatory cell surface molecules on lymphocytes. However, AKNA cDNA probes hybridize with multiple transcripts, suggesting either the existence of other homologous genes or a complex regulation op...
journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::By using the methodology of both wet and dry biology (i.e., RT-PCR and cycle sequencing, and biocomputational technology, respectively) and the data obtained through the Genome Projects, we have cloned Xenopus laevis SOD2 (MnSOD) cDNA and determined its nucleotide sequence. These data and the deduced protein primary s...
journal_title:DNA and cell biology
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abstract::In the rat, the growth hormone receptor (GH-R) gene generates two transcripts, one encoding the transmembrane GH-R, and a shorter one encoding the GH-binding protein (GH-BP). These transcripts exhibit a high degree of heterogeneity in their 5'-untranslated regions (5'-UTRs). Some of the exons encoding these 5'-UTR var...
journal_title:DNA and cell biology
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abstract::Endothelin-converting enzyme-1 (ECE1) plays a key role in the development of a subset of neural crest lineages such as cardiogenesis. Genetic variants of ECE1 C338A (rs213045) and T839G (rs213046) have been shown to alter ECE1 expression. This observation led us to hypothesize that two polymorphisms might influence th...
journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
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abstract::Duchenne muscular dystrophy is the most common and severe form of progressive muscular dystrophy. Previous results showed an increased survival in double knockout mice (dko) when treated with adipose-derived CD146+ cells. In this study, we analyzed the effect of CD146+ cells compared to mesenchymal stem/stromal cells ...
journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
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journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::The recent explosion of scientific and technical knowledge in the field of molecular biology has allowed us to make important advances in our understanding of the molecular basis of many human diseases. This technology has now entered the clinical laboratory where identification of specific genetic sequences can aid i...
journal_title:DNA and cell biology
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更新日期:1993-06-01 00:00:00
abstract::A simple, efficient procedure for cross-linking two complementary oligonucleotides, which does not require chemical modification of either oligonucleotide, is described. One of the oligonucleotides is first converted to the 5'-phosphorothioate derivative with polynucleotide kinase. It is then incubated with its comple...
journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
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abstract::In an effort to develop a more effective genetic immunization strategy for HIV, we developed an HIV-2 env DNA vaccine and evaluated three adjuvant formulations. The gp140 gene from HIV-2(UC2 )was synthesized using mammalian codons and cloned into a plasmid vector that expresses eukaryotic genes at high levels. We foun...
journal_title:DNA and cell biology
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更新日期:2004-02-01 00:00:00
abstract::Apoptotic endonuclease G (EndoG) is responsible for DNA fragmentation both during and after cell death. Previous studies demonstrated that genetic inactivation of EndoG is cytoprotective against various pro-apoptotic stimuli; however, specific inhibitors for EndoG are not available. In this study, we have developed a ...
journal_title:DNA and cell biology
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abstract::Accumulating evidence has suggested that microRNAs play important roles in the development of hepatocellular carcinoma (HCC) and are involved in drug resistance. miR-21-5p was overexpressed in a variety of cancers and promoted the tumorigenesis; however, the function of miR-21-5p in HCC still remains unknown. In this ...
journal_title:DNA and cell biology
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abstract::The Egr-1 (zfp-6) gene encodes a zinc-finger-containing nuclear protein that is rapidly and transiently induced in quiescent cells treated with mitogens. We have constructed baculovirus vectors that synthesize mouse Egr-1 protein initiating at two putative ATG start sites. The ATG site producing the larger protein (Mr...
journal_title:DNA and cell biology
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abstract::CD95 (Fas/APO-1)-mediated apoptosis appears to be regulated by positive and negative signaling molecules. A human CD40/CD95 chimeric receptor was stably transfected into CD95-expressing human Jurkat T cells, and signaling through native and chimeric CD95 was compared in the same cell type to assess contributions of th...
journal_title:DNA and cell biology
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abstract::The breast cancer gene 1 (BRCA1) is a tumor suppressor, and mutations or epigenetic inactivation will increase the risk of breast cancer oncogenesis. The current research aimed to explore the relationship between BRCA1 expression, prognosis, and tumor immunity in hepatocellular carcinoma (HCC). In this study, BRCA1 ex...
journal_title:DNA and cell biology
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abstract::The major surface promoter of human hepatitis B virus can produce three distinct groups of S transcripts. The initiation sites of these transcripts are in close proximity. Encompassing the ATG for the middle surface protein, the largest S transcript (+1) encodes the middle surface protein whereas the other two (+20 an...
journal_title:DNA and cell biology
pub_type: 杂志文章
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journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::Epulopiscium sp. type B, a member of the Firmicutes, is a large (up to 300 microm), cigar-shaped bacterial symbiont of surgeonfish that propagates itself by forming multiple intracellular offspring. This unusual form of reproduction is an apparent modification of a developmental program used by some Firmicutes to prod...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0801
更新日期:2009-02-01 00:00:00
abstract::Studies of the epigenome have attracted little interest in nephrology, especially in uremia. Several lines of evidence have suggested that there are links between genomic DNA hypomethylation and cardiovascular complications in uremia patients. However, to date, our knowledge about the alterations in histone methylatio...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1076
更新日期:2011-03-01 00:00:00
abstract::It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-de...
journal_title:DNA and cell biology
pub_type: 杂志文章
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.25
更新日期:1995-01-01 00:00:00
abstract::The present study was to explore whether the polymorphisms of FAM167 A-BLK region are associated with the susceptibility to autoimmune thyroid disease (AITD). The second sequencing technology was undertaken for seven tag loci mapping of the FAM167 A-BLK region, namely, rs11250144, rs2618431, rs4840568, rs13277113, rs2...
journal_title:DNA and cell biology
pub_type: 杂志文章
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abstract::Sestrin2 is involved in a different cellular response to stress conditions. However, the function of Sestrin2 in the cardiovascular system remains unknown. In the present study, we tested whether Sestrin2 has a beneficial effect on macrophage cell apoptosis induced by oxidized low-density lipoprotein (oxLDL). We found...
journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00