BRCA1 Is a Novel Prognostic Indicator and Associates with Immune Cell Infiltration in Hepatocellular Carcinoma.


:The breast cancer gene 1 (BRCA1) is a tumor suppressor, and mutations or epigenetic inactivation will increase the risk of breast cancer oncogenesis. The current research aimed to explore the relationship between BRCA1 expression, prognosis, and tumor immunity in hepatocellular carcinoma (HCC). In this study, BRCA1 expression was analyzed via multiple online databases and its association with clinical characteristics, prognosis and genetic alterations was identified using the original The Cancer Genome Atlas-liver hepatocellular carcinoma cohorts. DNA methylation sites and their prognostic values were analyzed using MethSurv. The correlations between BRCA1 and immune infiltration were investigated via Tumor Immune Estimation Resource. As results, BRCA1 was significantly upregulated in tumor tissues in multiple HCC cohorts. Besides, high BRCA1 expression was correlated with race, advanced T stage, clinical stage, poor tumor grade, MSI status, and worse prognosis. Notably, BRCA1 expression was positively correlated with infiltration levels of B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells. The current findings imply that BRCA1 is associated with prognosis and immune infiltration, laying foundations for in-depth research on the role of BRCA1 in HCC.


DNA Cell Biol


DNA and cell biology


Mei J,Wang R,Xia D,Yang X,Zhou W,Wang H,Liu C




Has Abstract


2020-10-01 00:00:00












  • Aberrant expression pattern of replication-dependent histone h3 subtype genes in human tumor cell lines.

    abstract::We have determined the expression pattern of all 11 human replication-dependent histone H3 genes in three fetal human tissues (bladder, liver, and lung) and in eight human cell lines by RNase protection assay. In the fetal human tissues, all 11 genes were expressed to a varied extent. However, the relative contributio...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Koessler H,Doenecke D,Albig W

    更新日期:2003-04-01 00:00:00

  • Satellite-tagged transcribing sequences in Bubalus bubalis genome undergo programmed modulation in meiocytes: possible implications for transcriptional inactivation.

    abstract::We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshybridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chattopadhyay M,Gangadharan S,Kapur V,Azfer MA,Prakash B,Ali S

    更新日期:2001-09-01 00:00:00

  • Identification of a new cellular protein that can interact specifically with DAN.

    abstract::Recently, we demonstrated that the DAN gene product contains a growth- and/or a tumor-suppressive activity in vitro. In the present work, using a yeast two-hybrid system, we searched for cellular proteins that can associate with the DAN gene product. A cDNA clone, termed DA41, was initially isolated from an adult rat ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Ozaki T,Hishiki T,Toyama Y,Yuasa S,Nakagawara A,Sakiyama S

    更新日期:1997-08-01 00:00:00

  • Evaluation of genetic immunization adjuvants to improve the effectiveness of a human immunodeficiency virus type 2 (HIV-2) envelope DNA vaccine.

    abstract::In an effort to develop a more effective genetic immunization strategy for HIV, we developed an HIV-2 env DNA vaccine and evaluated three adjuvant formulations. The gp140 gene from HIV-2(UC2 )was synthesized using mammalian codons and cloned into a plasmid vector that expresses eukaryotic genes at high levels. We foun...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Locher CP,Witt SA,Ashlock BM,Levy JA

    更新日期:2004-02-01 00:00:00

  • Apoptosis through CD95 (Fas/APO-1), but not a CD40/CD95 chimeric receptor, is inhibited by phorbol-12-myristate-13-acetate.

    abstract::CD95 (Fas/APO-1)-mediated apoptosis appears to be regulated by positive and negative signaling molecules. A human CD40/CD95 chimeric receptor was stably transfected into CD95-expressing human Jurkat T cells, and signaling through native and chimeric CD95 was compared in the same cell type to assess contributions of th...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Rudert F,Wang Y,Lindridge E,Watson J

    更新日期:1997-02-01 00:00:00

  • Glandular kallikreins of the cotton-top tamarin: molecular cloning of the gene encoding the tissue kallikrein.

    abstract::The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Olsson AY,Persson AM,Valtonen-André C,Lundwall A

    更新日期:2000-12-01 00:00:00

  • Identification of the Novel Target Genes for Osteosarcoma Therapy Based on Comprehensive Bioinformatic Analysis.

    abstract::Osteosarcoma is one of the most common primary malignant tumors of the bone and tends to develop in teenage years. Although multitreatments for the diagnosis and therapy of osteosarcoma have been developed, there are still needs of new methods to prevent and treat the osteosarcoma. Here, we performed bioinformatic ana...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Zhou X,Fan Y,Ye W,Jia B,Yang Y,Liu Y

    更新日期:2020-07-01 00:00:00

  • Retrospective DNA analysis using fixed tissue specimens.

    abstract::The recent explosion of scientific and technical knowledge in the field of molecular biology has allowed us to make important advances in our understanding of the molecular basis of many human diseases. This technology has now entered the clinical laboratory where identification of specific genetic sequences can aid i...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Crisan D,Mattson JC

    更新日期:1993-06-01 00:00:00

  • Cloning and characterization of two human polyspecific organic cation transporters.

    abstract::Previously we cloned a polyspecific transporter from rat (rOCT1) that is expressed in renal proximal tubules and hepatocytes and mediates electrogenic uptake of organic cations with different molecular structures. Recently a homologous transporter from rat kidney (rOCT2) was cloned but not characterized in detail. We ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Gorboulev V,Ulzheimer JC,Akhoundova A,Ulzheimer-Teuber I,Karbach U,Quester S,Baumann C,Lang F,Busch AE,Koepsell H

    更新日期:1997-07-01 00:00:00

  • The effect of progestins on prolactin receptor gene transcription in human breast cancer cells.

    abstract::The sex steroid hormone progesterone modulates the developmental and lactogenic activity of prolactin in the mammary gland. Regulation of the level of prolactin receptor (PRLR) provides one possible mechanism by which this may occur, prompting this investigation of the molecular mechanisms involved in progestin regula...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Ormandy CJ,Graham J,Kelly PA,Clarke CL,Sutherland RL

    更新日期:1992-12-01 00:00:00

  • Regenerative medicine for diseases of the head and neck: principles of in vivo regeneration.

    abstract::The application of endogenous regeneration in regenerative medicine is based on the concept of inducing regeneration of damaged or lost tissues from residual tissues in situ. Therefore, endogenous regeneration is also termed in vivo regeneration as opposed to mechanisms of ex vivo regeneration which are applied, for e...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Löwenheim H

    更新日期:2003-09-01 00:00:00

  • MicroRNAs in viral replication and pathogenesis.

    abstract::MicroRNAs (miRNAs) are an important class of small, noncoding, regulatory RNAs found to be involved in regulating a wide variety of important cellular processes by the sequence-specific inhibition of gene expression. Viruses have evolved a number of mechanisms to take advantage of the regulatory potential of this high...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Dykxhoorn DM

    更新日期:2007-04-01 00:00:00

  • miRNA-106a Promotes Breast Cancer Cell Proliferation, Clonogenicity, Migration, and Invasion Through Inhibiting Apoptosis and Chemosensitivity.

    abstract::To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated w...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: You F,Luan H,Sun D,Cui T,Ding P,Tang H,Sun D

    更新日期:2019-02-01 00:00:00

  • Expression of the proto-oncogene Axl in renal cell carcinoma.

    abstract::In this investigation, we examined the role of the Axl proto-oncogene in renal cell carcinoma (RCC). Axl is a tyrosine kinase receptor implicated in myeloid leukogenesis, and has been found to be overexpressed in lung cancers and breast cancers. Axl has been described to act as a mitogenic factor along with its ligand...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chung BI,Malkowicz SB,Nguyen TB,Libertino JA,McGarvey TW

    更新日期:2003-08-01 00:00:00

  • The UDP glucuronosyltransferase gene superfamily: suggested nomenclature based on evolutionary divergence.

    abstract::A nomenclature system for the UDP glucuronosyltransferase superfamily is proposed, based on divergent evolution of the genes. A total of 26 distinct cDNAs in five mammalian species have been sequenced to date. Comparison of the deduced amino acid sequences leads to the definition of two families and a total of three s...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Burchell B,Nebert DW,Nelson DR,Bock KW,Iyanagi T,Jansen PL,Lancet D,Mulder GJ,Chowdhury JR,Siest G

    更新日期:1991-09-01 00:00:00

  • In vitro and in silico cloning of Xenopus laevis SOD2 cDNA and its phylogenetic analysis.

    abstract::By using the methodology of both wet and dry biology (i.e., RT-PCR and cycle sequencing, and biocomputational technology, respectively) and the data obtained through the Genome Projects, we have cloned Xenopus laevis SOD2 (MnSOD) cDNA and determined its nucleotide sequence. These data and the deduced protein primary s...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Purrello M,Di Pietro C,Ragusa M,Pulvirenti A,Giugno R,Di Pietro V,Emmanuele G,Travali S,Scalia M,Shasha D,Ferro A

    更新日期:2005-02-01 00:00:00

  • No association of Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in susceptibility to gallbladder cancer.

    abstract::Gallbladder carcinoma (GBC) is a leading cause of cancer deaths in north India. Evidence has highlighted the role of abnormal DNA methylation patterns on inappropriate gene expression in development and progression of various cancers. 5,10-Methylenetetrahydrofolate reductase (MTHFR) plays a major role in provision of ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Srivastava A,Pandey SN,Pandey P,Choudhuri G,Mittal B

    更新日期:2008-03-01 00:00:00

  • Isolation of a yeast gene encoding a protein homologous to the human Tat-binding protein TBP-1.

    abstract::We have cloned a putative yeast homolog of the gene encoding the human Tat-binding protein, TBP-1. The gene termed TBPY encodes a 45,243-dalton protein displaying a heptad repeat of hydrophobic amino acids reminiscent of a leucine zipper. Secondary structure predictions suggest the possibility of formation of an amphi...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Goyer C,Lee HS,Malo D,Sonenberg N

    更新日期:1992-10-01 00:00:00

  • The effect of DYS-14 copy number variations on extracellular fetal DNA quantification in maternal circulation.

    abstract::The aims of our research involved to investigate DYS-14 copy number variations in healthy males, to quantify extracellular DNA in maternal circulation in normal versus complicated pregnancies, and to study variations in the DYS-14 copy number in extracellular male fetal DNA. Fifty-five healthy males, 43 uncomplicated ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Hromadnikova I,Benesova M,Zejskova L,Stehnova J,Doucha J,Sedlacek P,Dlouha K,Krofta L

    更新日期:2009-07-01 00:00:00

  • Dysregulation of CD4(+) T Cell Subsets in Intracranial Aneurysm.

    abstract::Intracranial aneurysms (IAs) and potential IA rupture are one of the direct causes of permanent brain damage and mortality. Interestingly, the major risk factors of IA development, including hemodynamic stress, hypertension, smoking, and genetic predispositions, are closely associated with a proinflammatory immune sta...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Zhang HF,Zhao MG,Liang GB,Yu CY,He W,Li ZQ,Gao X

    更新日期:2016-02-01 00:00:00

  • Efficient transfection and expression of heterologous genes in PC12 cells.

    abstract::The PC12 pheochromocytoma cell line has been a favorite model system for cell and neurobiologists, but has proven relatively refractory to standard DNA transfection methods. We have found that the cationic lipid "lipofectin" provides a simple, gentle, and nontoxic procedure that vastly improves transfection efficienci...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Muller SR,Sullivan PD,Clegg DO,Feinstein SC

    更新日期:1990-04-01 00:00:00

  • Evaluation of a putative vitamin D response element in the avian calcium binding protein gene.

    abstract::A primary response of the avian intestine to 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] is increased synthesis of a 28-kD calcium-binding protein, calbindin-D28k (CaBP). This study examined whether 1,25-(OH)2D3 regulates CaBP gene transcription by an interaction of the vitamin D receptor (VDR) with a vitamin D-responsive...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Macdonald PN,Whitfield GK,Haussler CA,Hocker AM,Haussler MR,Komm BS

    更新日期:1992-06-01 00:00:00

  • IL-5-Induced Eosinophils Suppress the Growth of Leishmania amazonensis In Vivo and Kill Promastigotes In Vitro in Response to Either IL-4 or IFN-gamma.

    abstract::In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Watanabe Y,Hamaguchi-Tsuru E,Morimoto N,Nishio Y,Yagyu K,Konishi Y,Tominaga M,Miyazaki J,Furuya M,Tominaga A

    更新日期:2004-07-01 00:00:00

  • Electroporation of the vasculature and the lung.

    abstract::Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for s...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审


    authors: Dean DA

    更新日期:2003-12-01 00:00:00

  • Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.

    abstract::A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence com...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Wagner S,Freudenstein J,Scheit KH

    更新日期:1990-07-01 00:00:00

  • HGF Gene Delivering Alginate/Galactosylated Chitosan Sponge Scaffold for Three-Dimensional Coculture of Hepatocytes/3T3 Cells.

    abstract::Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Gao C,Yang Y,Zhang Y,Qian M,Yang J

    更新日期:2020-03-01 00:00:00

  • Point mutation of the ras oncogene in human ovarian cancer.

    abstract::The ras oncogene exists in a variety of human cancers, including carcinomas of bladder, breast, colon, kidney, liver, lung, ovary, pancreas, and stomach. The ras genes acquire transforming activity either by enhanced expression or by a single point mutation. A single base-pair mutation at specific sites within ras gen...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chien CH,Chow SN

    更新日期:1993-09-01 00:00:00

  • Investigation of polymorphic variants of PPARD and APOE genes in Turkish coronary heart disease patients.

    abstract::The aim of this study was to determine the role of polymorphic variants of apolipoprotein E (APOE) and peroxisome proliferator-activated receptor delta (PPARD) genes in the development of coronary heart disease (CHD), and the PPARD and APOE gene-gene interaction in a Turkish population. This study was carried out usin...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Yılmaz-Aydogan H,Kucukhuseyin O,Kurnaz O,Akadam-Teker B,Kurt O,Tekeli A,Ozturk O,Isbir T

    更新日期:2012-05-01 00:00:00

  • Association Between AXIN1 Gene Polymorphisms and Dilated Cardiomyopathy in a Chinese Han Population.

    abstract::Dilated cardiomyopathy (DCM) is a common type of cardiomyopathy. The pathogenesis of DCM remains unclear and involves varied genes. AXIN1 is a crucial gene in regulating various functions in cells, it encodes protein Axin1, which regulates the assembly and disassembly of β-catenin destruction complex. In addition, Wnt...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Li K,Zhong Y,Peng Y,Zhou B,Wang Y,Li Q,Zhang Y,Song H,Rao L

    更新日期:2019-05-01 00:00:00

  • Induction of tetravalent protective immunity against four dengue serotypes by the tandem domain III of the envelope protein.

    abstract::In the present study, the domain IIIs of all four dengue virus (DENV) serotypes were connected sequentially to construct the tandem domain III. The resulting DNA fragment was then cloned into pBAD/Topo ThioFusion plasmid. After induction, the Escherichia coli expression protein was purified and used to immunize BALB/c...

    journal_title:DNA and cell biology

    pub_type: 杂志文章


    authors: Chen S,Yu M,Jiang T,Deng Y,Qin C,Qin E

    更新日期:2007-06-01 00:00:00