Abstract:
:Denaturing gradient gel electrophoresis (DGGE) was used to examine error rates and mutations induced by native (wt) and exonuclease-deficient (exo-) Deep Vent DNA polymerases during DNA amplification by polymerase chain reaction (PCR), in the presence or absence of the T4 bacteriophage gene 32 protein (gp32).gp32 was found to decrease the error rate of the wt, but not that of the exo-, Deep Vent. The average errors per base duplication for the native form were 8.0 x 10(-5) and 6.0 x 10(-5) in the absence and presence of gp32, respectively. For the exo- form, the error rates were 2.0 x 10(-4) and 2.2 x 10(-4) errors per base duplication in the absence and presence of gp32, respectively. Examination of mutations produced by native Deep Vent showed that A/T to G/C transition predominated, consistent with the results of our earlier studies with DNA polymerases derived from other thermophilic bacteria. These results indicate that PCR with high fidelity can be achieved by using wt Deep Vent in combination with gp32.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Huang H,Keohavong Pdoi
10.1089/dna.1996.15.589subject
Has Abstractpub_date
1996-07-01 00:00:00pages
589-94issue
7eissn
1044-5498issn
1557-7430journal_volume
15pub_type
杂志文章abstract::In this study we report main properties of Harbinger DNA transposons identified in protists, plants, insects, worms, and vertebrates. This is the first superfamily of eukaryotic DNA transposons where all autonomous transposons, even those that are hosted by species from different kingdoms, encode two proteins: a super...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454904323090949
更新日期:2004-05-01 00:00:00
abstract::Dilated cardiomyopathy (DCM) is a common type of cardiomyopathy. The pathogenesis of DCM remains unclear and involves varied genes. AXIN1 is a crucial gene in regulating various functions in cells, it encodes protein Axin1, which regulates the assembly and disassembly of β-catenin destruction complex. In addition, Wnt...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4567
更新日期:2019-05-01 00:00:00
abstract::Apoptosis, which is usually accompanied by DNA degradation, is important not only for the homeostasis of metazoans but also for mammalian development. If DNA is not properly degraded in these processes, it can cause diverse diseases, such as anemia, cataracts, and some autoimmune diseases. A large effort has been made...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2007.0691
更新日期:2008-05-01 00:00:00
abstract::Phytoplasmas are nonculturable cell wall-less, obligate intracellular pathogens of plants and insect vectors. In their descent from walled bacterial ancestors, phytoplasmas underwent massive genome reduction, resulting in some of the smallest cellular genomes known in nonsymbiotic bacteria. While requirements for in v...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.832
更新日期:2005-12-01 00:00:00
abstract::The gene encoding the mouse cellular retinoic acid-binding protein (CRABP) has been isolated from a mouse genomic library and its structure has been determined. This gene spans approximately 10.5 kb and consists of four exons encoding 24, 59, 38, and 16 amino acid residues, respectively. This gene structure is very si...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.471
更新日期:1990-09-01 00:00:00
abstract::We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.1063
更新日期:1996-12-01 00:00:00
abstract::Gulf War Illness (GWI) affects about 25% of Persian Gulf veterans with a cluster of chronic symptoms, including immune dysfunction and neurological issues. Recent studies implicate gene expression changes in immune function to be associated with GWI. Since DNA methylation can regulate such changes in gene expression, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4469
更新日期:2019-06-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignancy with relatively high incidence and poor prognosis. RNA-binding proteins (RBPs) were reported to be dysregulated in multiple cancers and were closely associated with tumor initiation and progression. However, an integrated analysis of the roles of RBPs in HN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5641
更新日期:2020-10-01 00:00:00
abstract::Interleukin-6 (IL-6) signaling may play a causal role in the development of coronary heart disease. However, the relationship between IL-6 genotypes and plasma levels of IL-6 appears to be complex. To help clarify the inconsistent findings, we conducted a meta-analysis of the published genetic association studies of t...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.2148
更新日期:2013-11-01 00:00:00
abstract::Recently, we demonstrated that the DAN gene product contains a growth- and/or a tumor-suppressive activity in vitro. In the present work, using a yeast two-hybrid system, we searched for cellular proteins that can associate with the DAN gene product. A cDNA clone, termed DA41, was initially isolated from an adult rat ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.985
更新日期:1997-08-01 00:00:00
abstract::Deer antlers are amazing natural appendages that grow faster than any other known mammalian bone. Antler growth occurs at the tip and is initially cartilage, which is later replaced by bone tissue. However, little is known regarding the precise role of cooperation between cell lineages and functional genes in regulati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2017.3885
更新日期:2018-01-01 00:00:00
abstract::The ras oncogene exists in a variety of human cancers, including carcinomas of bladder, breast, colon, kidney, liver, lung, ovary, pancreas, and stomach. The ras genes acquire transforming activity either by enhanced expression or by a single point mutation. A single base-pair mutation at specific sites within ras gen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.623
更新日期:1993-09-01 00:00:00
abstract::Chromosomal gain on 1q23-24 is a cytogenetic finding found in approximately 30% of bladder tumors. Currently, no defined or candidate tumor-associated genes from this region have been identified. The objective of this study was to identify and quantitate the expression of putative cancer genes located at this chromoso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902760599681
更新日期:2002-10-01 00:00:00
abstract::The promoters of mammalian striated muscle actin gene contain binding sites for a number of transcription factors. Examples are the CArG boxes, which bind a protein identical to or related to serum response factor (SRF), E boxes, which bind myogenic determination factors such as MyoD and myogenin, and -CCGCCC- motifs,...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.609
更新日期:1995-07-01 00:00:00
abstract::Previous studies showed that when growth hormone-releasing hormone (GHRH) was administered to either pregnant rats or pigs as a plasmid-mediated therapy, pituitary weight, somatotroph and lactotroph numbers, and postnatal growth rate of the offspring increased. To determine if these responses resulted from direct effe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.429
更新日期:2006-08-01 00:00:00
abstract::The serotonin (5-hydroxytryptamine) transporter (5HTt) gene has been reported to be associated with suicidal behavior. In this study, we have investigated the 5' promoter region (i.e., 5HTt gene-linked polymorphic region [5-HTTLPR]) and a 17-base pair variable number of tandem repeats polymorphism in the 5HTt gene for...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1031
更新日期:2010-08-01 00:00:00
abstract::The abnormal function of O(6)-methylguanine-DNA methyltransferase (MGMT) is reported to be associated with the occurrence of various tumors and malignant tumor progression. However, little evidence is available to describe its role in esophageal carcinogenesis. To address this issue, we constructed a stable MGMT-silen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1318
更新日期:2012-05-01 00:00:00
abstract::Long noncoding RNA (lncRNA) was closely attached to various cancers according to previous studies. In this study we aimed to investigate an lncRNA signature with prognostic value of over survival (OS) outcomes of gastric cancer (GC). Profiles of mRNAs expression and clinical information of 381 GC tissues and 32 nontum...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4832
更新日期:2019-09-01 00:00:00
abstract::In this study, we isolated two cDNA molecules encoding putative glutamate receptor subunits, fGluR1 alpha and fGluR1 beta, from an Oreochromis sp. brain cDNA library by hybridizing with the glutamate receptor cDNA, fGluR2 beta, of the same fish. The deduced amino acid sequence of the fGluR1 alpha consists of 908 resid...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.717
更新日期:1996-09-01 00:00:00
abstract::Design and synthesis of new small molecules binding to double-stranded RNA necessitate complete understanding of the molecular aspects of the binding of many existing molecules. Toward this goal, in this work we evaluated the biophysical aspects of the interaction of a DNA intercalator (proflavine) and a minor groove ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2008.0838
更新日期:2009-04-01 00:00:00
abstract::Nd1-S is the nuclear-localizing short variant form of Nd1 (Ivns1abp) encoding a Kelch family transcription factor. While the function of Nd1 has been investigated in the context of metastasis and doxorubicin-induced cardiotoxicity, little is known about its role in hematopoiesis. In this study, we investigated the fun...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4206
更新日期:2018-10-01 00:00:00
abstract::We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracister...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.319
更新日期:1993-05-01 00:00:00
abstract::The human OZF gene encodes a protein consisting essentially of zinc finger motifs of the Krüppel type. Evolutionary studies revealed amplification of the bovine OZF gene in cattle. Domestic cattle includes two major subspecies: taurine (Bos primigenius taurus) and zebu (B. p. indicus). Amplified sequences were found i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.83
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:This case-control study was conducted in order to evaluate the potential role of polymorphic genes encoding enzymes involved in estrogen biosynthesis (CYP19A1) and metabolism (GSTM1, GSTT1, and GSTP1), and their action in modulating individual susceptibility to breast cancer. METHODS:Genomic DNA was extracted ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1538
更新日期:2012-06-01 00:00:00
abstract::Polymerase chain reaction [PCR, reverse transcriptase-PCR (RT-PCR)] has been used to amplify the mRNA subspecies of the plasma membrane calcium pump isoform 1 (PMCA1) in total RNA extracted from hamster tissues. Two primers were synthesized that encompass the site at which a 154-bp exon is included totally (PMCA1a), p...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.435
更新日期:1993-06-01 00:00:00
abstract::We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshybridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901317095007
更新日期:2001-09-01 00:00:00
abstract::Sestrin2 is involved in a different cellular response to stress conditions. However, the function of Sestrin2 in the cardiovascular system remains unknown. In the present study, we tested whether Sestrin2 has a beneficial effect on macrophage cell apoptosis induced by oxidized low-density lipoprotein (oxLDL). We found...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2627
更新日期:2015-04-01 00:00:00
abstract::In the current study, we evaluated the potential effect of a novel sphingosine kinase 1 (SphK1) activator, K6PC-5, on oxygen-glucose deprivation (OGD)/reoxygenation-induced damages to myocardial cells. We demonstrated that K6PC-5 increased intracellular sphingosine-1-phosphate (S1P) content and remarkably inhibited OG...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2959
更新日期:2015-11-01 00:00:00
abstract::In a systematic approach to identify interferon-gamma (IFN-gamma)-regulated host effector molecules, we found several members of the 65 kDa guanylate-binding proteins (GBPs) highly upregulated. During extensive characterization of these guanosine triphosphatases (GTPases), we identified discrepancies between the clone...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0637
更新日期:2007-12-01 00:00:00