Use of the polymerase chain reaction for the detection of alternatively spliced mRNAs of plasma membrane calcium pump.

abstract：:Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared fro...

journal_title：DNA and cell biology

authors： Narahari J,Roman A

更新日期：2002-02-01 00:00:00

abstract：:DNA condensed agents can improve the transfection efficiency of the cationic liposome delivery system. However, various condensed agents have distinct transfection efficiency and cellular cytotoxicity. The object of this study was to screen the optimal agents with the high transfection efficiency and low cytotoxicity ...

journal_title：DNA and cell biology

authors： Wang J,Ye X,Ni H,Zhang J,Ju S,Ding W

更新日期：2019-10-01 00:00:00

abstract：:Polyhomeotic is a member of the Polycomb group of genes. The products of this group are chromatin-associated proteins that act together as multimeric complexes. These proteins are required for the maintenance of target gene repression in a permanent and heritable manner during development. In order to better understan...

journal_title：DNA and cell biology

authors： Netter S,Faucheux M,Théodore L

更新日期：2001-08-01 00:00:00

abstract：:The incidence of premature ovarian failure (POF), a condition causing amenorrhea and hypergonadotropic hypoestrogenism in women before the age of 40, has been increasing in recent years. As an irreversible pathological change, improved treatment strategies for this disease are urgently needed. In this study, a type of...

journal_title：DNA and cell biology

authors： Liu T,Qin W,Huang Y,Zhao Y,Wang J

更新日期：2013-12-01 00:00:00

abstract：:In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...

journal_title：DNA and cell biology

authors： Watanabe Y,Hamaguchi-Tsuru E,Morimoto N,Nishio Y,Yagyu K,Konishi Y,Tominaga M,Miyazaki J,Furuya M,Tominaga A

更新日期：2004-07-01 00:00:00

abstract：:ERGIC2 (formerly known as PTX1) is a gene identified by subtractive hybridization on the basis that it is expressed in normal human prostate, but not in prostate carcinoma. It is unrelated to the gene encoding pituitary homeobox protein (Ptx1 or Pitx1), which regulates pituitary hormone gene expression. Based on seque...

journal_title：DNA and cell biology

authors： Kwok SC,Kumar S,Dai G

更新日期：2014-02-01 00:00:00

abstract：:Viral factor has been implicated in the etiopathogenesis of vitiligo. To elucidate the effects of viral double-stranded RNA (dsRNA) on melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were treated with synthetic viral dsRNA analog poly(I:C). The results demonstrated that ...

journal_title：DNA and cell biology

authors： Wang S,Liu D,Jin R,Zhu Y,Xu A

更新日期：2015-06-01 00:00:00

abstract：:CD95 (Fas/APO-1)-mediated apoptosis appears to be regulated by positive and negative signaling molecules. A human CD40/CD95 chimeric receptor was stably transfected into CD95-expressing human Jurkat T cells, and signaling through native and chimeric CD95 was compared in the same cell type to assess contributions of th...

journal_title：DNA and cell biology

authors： Rudert F,Wang Y,Lindridge E,Watson J

更新日期：1997-02-01 00:00:00

abstract：:We have further characterized the most distal of the three alpha-fetoprotein (AFP) enhancers required for expression of the AFP gene in fetal hepatocytes and yolk sac endodermal cells. Almost total rat AFP enhancer 3 (E3) activity is driven by a 160-bp fragment at -6 kb containing three target regions for nuclear prot...

journal_title：DNA and cell biology

authors： Thomassin H,Bois-Joyeux B,Delille R,Ikonomova R,Danan JL

更新日期：1996-12-01 00:00:00

abstract：:The human spermidine synthase (EC 2.5.1.16) gene was isolated from a genomic library constructed with DNA obtained from a human immunoglobulin G (IgG) myeloma cell line. Subsequent sequence analyses revealed that the gene comprised of 5,818 nucleotides from the cap site to the last A of the putative polyadenylation si...

journal_title：DNA and cell biology

authors： Myöhänen S,Kauppinen L,Wahlfors J,Alhonen L,Jänne J

更新日期：1991-07-01 00:00:00

abstract：:The human immunodeficiency virus type I (HIV-1) accessory protein Vpr has been associated with the induction of programmed cell death (apoptosis) and cell-cycle arrest. Studies have shown the apoptotic effect of Vpr on primary and established cell lines and on diverse tissues including the central nervous system (CNS)...

journal_title：DNA and cell biology

authors： Cheng X,Mukhtar M,Acheampong EA,Srinivasan A,Rafi M,Pomerantz RJ,Parveen Z

更新日期：2007-02-01 00:00:00

abstract：:A series of overlapping cDNAs coding for mouse prothrombin (coagulation factor II) have been isolated and the composite DNA sequence has been determined. The complete prothrombin cDNA is 1,987 bp in length [excluding the poly(A) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 61...

journal_title：DNA and cell biology

authors： Degen SJ,Schaefer LA,Jamison CS,Grant SG,Fitzgibbon JJ,Pai JA,Chapman VM,Elliott RW

更新日期：1990-09-01 00:00:00

abstract：:Our aim was to evaluate in a cohort of 125 Italian patients with type 2 diabetes (T2D), who underwent neurological evaluation, the possible differences in the number of mitochondrial DNA copies (mtDNA) comparing positive and negative patients for cardiovascular autonomic neuropathy (CAN) or diabetic peripheral neuropa...

journal_title：DNA and cell biology

authors： Latini A,Borgiani P,De Benedittis G,D'Amato C,Greco C,Lauro D,Novelli G,Spallone V,Ciccacci C

更新日期：2020-08-01 00:00:00

abstract：:Dengue virus serotype 3 (DENV-3) was associated with severe dengue epidemics in Thailand during 1973-1999. We studied Thai DENV-3 viruses isolated from hospitalized children in Bangkok with differing disease severity during that period. Viruses were sequenced at their 5' and 3' untranslated regions (UTRs), which are r...

journal_title：DNA and cell biology

authors： Pankhong P,Weiner DB,Ramanathan MP,Nisalak A,Kalayanarooj S,Nimmannitya S,Attatippaholkun W

更新日期：2009-10-01 00:00:00

abstract：:A highly conserved aspartic acid residue present in the third membrane-spanning region of adrenergic and muscarinic receptors is directly involved in ligand binding. The five cloned somatostatin receptor subtypes also contain this residue at the same relative position. To test whether Asp-124 of the rat somatostatin r...

journal_title：DNA and cell biology

authors： Nehring RB,Meyerhof W,Richter D

更新日期：1995-11-01 00:00:00

abstract：:My 45 years of studying the molecular biology of adeno-associated virus are recounted. Additional activities as a mentor, department chair, and medical school administrator are described, as are my activities in the public sphere, which involved national issues related to science policy and medical education. ...

journal_title：DNA and cell biology

authors： Berns KI

更新日期：2013-07-01 00:00:00

abstract：:alpha B-Crystallin, a member of the small heat shock family of proteins, is synthesized as a component of various developmental programs, in response to stress and in a number of pathological states. We have determined the complete structure of the alpha B-crystallin gene (6,806 bp encompassing 2,299 bp upstream from ...

journal_title：DNA and cell biology

authors： Srinivasan AN,Bhat SP

更新日期：1994-06-01 00:00:00

abstract：:We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshybridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS...

journal_title：DNA and cell biology

authors： Chattopadhyay M,Gangadharan S,Kapur V,Azfer MA,Prakash B,Ali S

更新日期：2001-09-01 00:00:00

abstract：:Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...

journal_title：DNA and cell biology

authors： Nusbaum NJ,Joseph PE

更新日期：1996-05-01 00:00:00

abstract：:The mammalian CYP2E1 gene encodes a cytochrome P450 enzyme that is markedly increased soon after birth. In vitro evidence suggests that the rat CYP2E1 gene is positively regulated by the transcription factor HNF-1 or a protein displaying DNA-binding properties similar to HNF-1. In contrast to newborn wild-type mice an...

journal_title：DNA and cell biology

authors： Gonzalez FJ,Liu SY,Kozak CA,Nebert DW

更新日期：1990-12-01 00:00:00

abstract：:Among environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is one of the most potent tumor promoters and teratogens known. The molecular mechanisms responsible for the biological activity of TCDD, however, remain largely unknown. In this report, we show that the first observable effects of TCDD...

journal_title：DNA and cell biology

authors： Puga A,Nebert DW,Carrier F

更新日期：1992-05-01 00:00:00

abstract：:Gene delivery from tissue engineering scaffold is a novel strategy in regulating long-term growth and function of cells in vitro culture. In this study, a hepatocyte growth factor plasmid/polyetherimide (pHGF/PEI) polyplex delivering alginate (AL)/galactosylated chitosan (GC) (pHGF/PEI-AL/GC) sponge scaffold was prepa...

journal_title：DNA and cell biology

authors： Gao C,Yang Y,Zhang Y,Qian M,Yang J

更新日期：2020-03-01 00:00:00

abstract：:In the rat, the growth hormone receptor (GH-R) gene generates two transcripts, one encoding the transmembrane GH-R, and a shorter one encoding the GH-binding protein (GH-BP). These transcripts exhibit a high degree of heterogeneity in their 5'-untranslated regions (5'-UTRs). Some of the exons encoding these 5'-UTR var...

journal_title：DNA and cell biology

authors： Domené HM,Cassorla F,Werner H,Roberts CT Jr,Leroith D

更新日期：1995-03-01 00:00:00

abstract：:A safe and effective adjuvant is important to develop vaccines against highly pathogenic avian influenza virus. Chitosan, a derivative from the natural amino polysaccharide chitin, has been proved to be an effective adjuvant for inactivated influenza virus vaccine. In this study, protective immunity in mice provided b...

journal_title：DNA and cell biology

authors： Chang H,Li X,Teng Y,Liang Y,Peng B,Fang F,Chen Z

更新日期：2010-09-01 00:00:00

abstract：:Leber congenital amaurosis (LCA) causes severe visual impairment and blindness very early in life. Mutant alleles of several genes acting in different pathways, of which all have critical roles for normal retinal function, were involved in LCA development. The purpose of this study was to use genome-wide genotyping to...

journal_title：DNA and cell biology

authors： Yücel-Yılmaz D,Tarlan B,Kıratlı H,Ozgül RK

更新日期：2014-12-01 00:00:00

abstract：:The long arm of chromosome 6 is frequently deleted in diverse human neoplasms. Our previous study showed a minimum deletion region between markers D6S1056 and D6S300 on chromosome 6q in primary prostate cancer (CaP). In this study, we further refined a 200-kb minimal region of deletion (6qTSG1) centered around D6S1013...

journal_title：DNA and cell biology

authors： Sun M,Srikantan V,Ma L,Li J,Zhang W,Petrovics G,Makarem M,Strovel JW,Horrigan SG,Augustus M,Sesterhenn IA,Moul JW,Chandrasekharappa S,Zou Z,Srivastava S

更新日期：2006-11-01 00:00:00

abstract：:The p53 tumor suppressor gene is frequently mutated in multiple human cancers, leading to loss of wild-type p53 (wt-p53)-dependent functions and tumorigenesis. p53 gene therapy is used to induce apoptosis in human cancer cells and tumors. Activation of nuclear factor kappa B (NF-kappaB) causes resistance to both chemo...

journal_title：DNA and cell biology

authors： Rath PC,Mukhopadhyay T

更新日期：2009-12-01 00:00:00

abstract：:Age-related macular degeneration (AMD) is a disease with multifactorial etiology characterized by irreversible loss of central visual acuity. The discovery of susceptive single-nucleotide polymorphisms (SNPs) has progressed our understanding of AMD. Complement factor H (CFH) gene Y402H polymorphism and high-temperatur...

journal_title：DNA and cell biology

authors： Soysal Y,Inan UÜ,Küsbeci T,Imirzalioğlu N

更新日期：2012-03-01 00:00:00

abstract：:Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...

journal_title：DNA and cell biology

authors： Müller WE,Perovic S,Wilkesman J,Kruse M,Müller IM,Batel R

更新日期：1999-12-01 00:00:00

abstract：:Disruption of cell-matrix interactions can lead to anoikis-apoptosis due to loss of matrix contacts. We previously showed that Nerve/glial antigen 2 (NG2) is a novel anoikis receptor. Specifically, overexpression of NG2 leads to anoikis propagation, whereas its suppression leads to anoikis attenuation. Interestingly, ...

journal_title：DNA and cell biology

authors： Joo NE,Miao D,Bermúdez M,Stallcup WB,Kapila YL

更新日期：2014-12-01 00:00:00