Abstract:
:Accumulating evidence has suggested that microRNAs play important roles in the development of hepatocellular carcinoma (HCC) and are involved in drug resistance. miR-21-5p was overexpressed in a variety of cancers and promoted the tumorigenesis; however, the function of miR-21-5p in HCC still remains unknown. In this study, our results showed that miR-21-5p was highly expressed in HCC tissues and cell lines. Notably, the level of miR-21-5p was relatively higher in cisplatin (DDP)-resistant HCC patients. Overexpression of miR-21-5p attenuated the inhibitory effect of DDP on the proliferation and apoptosis of HCC cells. Mechanistically, the luciferase report assay-identified FAS ligand (FASLG) was a direct target of miR-21-5p. Overexpression of miR-21-5p decreased both the mRNA and protein levels of FASLG in HCC cells. FASLG was downregulated in HCC tissues and was significantly negatively correlated with the expression of miR-21-5p. Restoring the expression of FASLG upregulated the chemosensitivity of HCC cells expressing miR-21-5p. In conclusion, our results demonstrated that miR-21-5p targeted FASLG and suppressed the sensitivity of HCC cells to DDP treatment.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Chen S,Yang C,Sun C,Sun Y,Yang Z,Cheng S,Zhuge Bdoi
10.1089/dna.2018.4529subject
Has Abstractpub_date
2019-08-01 00:00:00pages
865-873issue
8eissn
1044-5498issn
1557-7430journal_volume
38pub_type
杂志文章abstract::To study the complexity and structural characteristics of zinc finger proteins expressed during human hematopoiesis and to isolate novel regulators of blood cell development, a degenerate oligonucleotide probe specific for a consensus zinc finger peptide domain was used to isolate 63 cDNA clones for Krüppel-related zi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.125
更新日期:1995-02-01 00:00:00
abstract::Proopiomelanocortin (POMC) is expressed predominantly in the corticotrophic cells of the pituitary. Regulatory sequences required for the expression of the human (h) POMC gene were investigated using transient expression of hPOMC-CAT fusion genes in pituitary and nonpituitary cells in combination with DNase I footprin...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1993.12.527
更新日期:1993-07-01 00:00:00
abstract::Acetate and β-hydroxybutyrate (BHBA) are the predominant substrates for de novo fatty acid (FA) synthesis in mammary gland of dairy cow. To investigate the nutrigenomic role of acetate and BHBA in bovine mammary epithelial cells during milk fat production, RNA sequencing (RNA-seq) transcriptomic analysis was used to i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4783
更新日期:2020-03-01 00:00:00
abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.613
更新日期:1998-07-01 00:00:00
abstract::Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites Ser* and Asn*...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.415
更新日期:1990-07-01 00:00:00
abstract::In a systematic approach to identify interferon-gamma (IFN-gamma)-regulated host effector molecules, we found several members of the 65 kDa guanylate-binding proteins (GBPs) highly upregulated. During extensive characterization of these guanosine triphosphatases (GTPases), we identified discrepancies between the clone...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0637
更新日期:2007-12-01 00:00:00
abstract::Host cell-derived danger-associated molecular patterns (DAMPs), such as the hemoglobin (Hb) can interact with the innate immune system either directly or through binding to pathogen-associated molecular patterns (PAMPs). Hemolysis occurs under various pathological conditions, leading to hemoglobinemia. In the extracel...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2012.1897
更新日期:2013-02-01 00:00:00
abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320908441
更新日期:2002-11-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::RUNX2 is a member of the RUNX family of transcription factors, also containing the RUNX1 and RUNX3 proteins. These factors control the expression of genes essential for proper development in many cell lineages. RUNX2 plays a crucial role in the proliferation and differentiation of osteoblasts, required for bone format...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2014.2688
更新日期:2015-05-01 00:00:00
abstract::Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main tra...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3535
更新日期:2017-03-01 00:00:00
abstract::In the current study, we evaluated the potential effect of a novel sphingosine kinase 1 (SphK1) activator, K6PC-5, on oxygen-glucose deprivation (OGD)/reoxygenation-induced damages to myocardial cells. We demonstrated that K6PC-5 increased intracellular sphingosine-1-phosphate (S1P) content and remarkably inhibited OG...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2959
更新日期:2015-11-01 00:00:00
abstract::The murine 18A2/mts1 and its human homolog h-mts1 (S100A4), encoding a Ca2+-binding protein belonging to the S-100 family, are associated with high invasive and metastatic potentials of murine tumors, human tumor cell lines in vitro, and human tumors growing as xenografts. The nm23 is a putative metastasis-suppressor ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.335
更新日期:1998-04-01 00:00:00
abstract::Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilti...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.6245
更新日期:2020-12-29 00:00:00
abstract::Apicomplexa is a phylum within the kingdom Protista that contains some of the most significant threats to public health. One of the members of this phylum, Toxoplasma gondii, is amenable to molecular genetic analyses allowing for the identification of factors critical for colonization and disease. A pathway found to b...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2009.0853
更新日期:2009-06-01 00:00:00
abstract::Mouse vimentin intermediate filaments (IFs) reconstituted in vitro were analyzed for their capacity to select certain DNA sequences from a mixture of about 500-bp-long fragments of total mouse genomic DNA. The fragments preferentially bound by the IFs and enriched by several cycles of affinity binding and polymerase c...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.209
更新日期:1996-03-01 00:00:00
abstract::The long arm of chromosome 6 is frequently deleted in diverse human neoplasms. Our previous study showed a minimum deletion region between markers D6S1056 and D6S300 on chromosome 6q in primary prostate cancer (CaP). In this study, we further refined a 200-kb minimal region of deletion (6qTSG1) centered around D6S1013...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.597
更新日期:2006-11-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) is known for its ability to infect immune cells, including T-cells and macrophages. The 96-amino acid Vpr, a virion-associated protein, is essential for viral replication in monocytes/macrophages and increases viral replication in primary and established T-cell lines. The Vp...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900314564
更新日期:2000-03-01 00:00:00
abstract::We have isolated and characterized a cDNA encoding a chicken beta homolog of c-erbA, or thyroid hormone receptor (TR). Chicken liver cDNA libraries were screened with a rat TR beta-1 cDNA probe, and several cDNA inserts were isolated and characterized. The sequence of one cDNA predicts a 369-amino-acid open reading fr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.211
更新日期:1991-04-01 00:00:00
abstract::To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4282
更新日期:2019-02-01 00:00:00
abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903322216671
更新日期:2003-05-01 00:00:00
abstract::Mutations in the mitochondrial genome (mtDNA) are associated with different types of cancer, specifically colorectal cancer (CRC). However, few studies have been performed on precancerous lesions, such as ulcerative colitis (UC) lesions and adenomatous polyps (AP). The aim of this study was to identify mtDNA mutations...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1186
更新日期:2011-11-01 00:00:00
abstract::DNA repair is reduced in patients suffering from systemic lupus erythematosus (SLE), and it can induce the production of autoreactive antibodies due to the accumulation of DNA damage and nucleoprotein that produce immunogenic antigens. The accumulations of anti-Ku and DNA-PKcs antibodies, which are involved in nonhomo...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2465
更新日期:2015-05-01 00:00:00
abstract::A simple, efficient procedure for cross-linking two complementary oligonucleotides, which does not require chemical modification of either oligonucleotide, is described. One of the oligonucleotides is first converted to the 5'-phosphorothioate derivative with polynucleotide kinase. It is then incubated with its comple...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.71
更新日期:1990-01-01 00:00:00
abstract::We describe a new vector system that allows efficient expression of heterologous proteins in transformed mouse L fibroblasts. This is due to its persistence at high copy numbers, achieved by a 370-bp amplification promoting element (muNTS1) derived from the nontranscribed spacer of murine rDNA. Copy number determinati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.437
更新日期:1994-04-01 00:00:00
abstract::The overexpression of prosurvival members of the Bcl-2 family is commonly associated with the enhanced malignancy of hematological tumors. There has been great interest in a novel set of agents that are able to mimic the function of the BH3 domain by binding to the groove of Bcl-2-like proteins and initiating the cell...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0675
更新日期:2008-03-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) may serve as potential molecular diagnostic markers to improve the capacity of earlier and more accurate diagnosis of dilated cardiomyopathy (DCM). We integrated five independent transcriptomic datasets (n = 504) from Gene Expression Omnibus for systematic identification of lncRNA-based d...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5670
更新日期:2020-11-03 00:00:00
abstract::A highly conserved aspartic acid residue present in the third membrane-spanning region of adrenergic and muscarinic receptors is directly involved in ligand binding. The five cloned somatostatin receptor subtypes also contain this residue at the same relative position. To test whether Asp-124 of the rat somatostatin r...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.939
更新日期:1995-11-01 00:00:00
abstract::The upstream transcription control region of the rat alpha-fetoprotein (AFP) gene was analyzed using transient expression of CAT genes in HepG2 cells which express the gene; H4C3 cells which repress the AFP gene but express the albumin gene; and four nonexpressing cell lines. Deletion analysis based on the DNA sequenc...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.525
更新日期:1991-09-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder characterized by overproduction of beta-amyloid (Abeta), which is formed from amyloid precursor protein (APP), with the subsequent pathologic deposition of Abeta in regions of the brain important for memory and cognition. Recently, vaccination of murine models o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490152717587
更新日期:2001-11-01 00:00:00