Abstract:
:We describe a new vector system that allows efficient expression of heterologous proteins in transformed mouse L fibroblasts. This is due to its persistence at high copy numbers, achieved by a 370-bp amplification promoting element (muNTS1) derived from the nontranscribed spacer of murine rDNA. Copy number determination showed that this sequence mediates a 40- to 800-fold amplification of the vector DNA in transfected L cells. High copy number was accompanied by increased expression levels of the reporter gene secreted alkaline phosphatase (SEAP). Analyzing the structural organization of multicopy plasmid DNA in mouse L cells revealed that plasmid DNA is integrated as reiterated head-to-tail concatamers into the chromosomal DNA. The vector described here can be used as a versatile high-copy expression system for heterologous proteins overcoming any limitation to enzyme-deficient cell lines.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Hemann C,Gärtner E,Weidle UH,Grummt Fdoi
10.1089/dna.1994.13.437subject
Has Abstractpub_date
1994-04-01 00:00:00pages
437-45issue
4eissn
1044-5498issn
1557-7430journal_volume
13pub_type
杂志文章abstract::Herpes simplex virus (HSV) is a neurotropic DNA virus with many favorable properties as a gene delivery vector. HSV is highly infectious, so HSV vectors are efficient vehicles for the delivery of exogenous genetic material to cells. Viral replication is readily disrupted by null mutations in immediate early genes that...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902762053864
更新日期:2002-12-01 00:00:00
abstract::Protein homeostasis in the endoplasmic reticulum (ER) in eukaryotic cells is maintained by a conserved quality control system named ER-associated degradation (ERAD). The ERAD system retains misfolded or unassembled polypeptides in the ER, retrotranslocates them into the cytosol for degradation by the ubiquitin proteas...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2014.2452
更新日期:2014-08-01 00:00:00
abstract::The family of G protein-coupled receptors (GPCRs) serves as the target for almost a third of currently marketed drugs, and provides the predominant mechanism through which extracellular factors transmit signals to the cell. The discovery of GPCRs with no known ligand has initiated a frenzy of research, with the aim of...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2005.24.54
更新日期:2005-01-01 00:00:00
abstract::Sufficient levels of gene expression are required for effective gene therapy. One of the major obstacles in gene therapy is the low transgene expression obtained from currently available vector systems. To address this issue, we employed a transcriptional amplifier strategy in a single construct to enhance transgene e...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.595
更新日期:2005-09-01 00:00:00
abstract::A series of overlapping cDNAs coding for mouse prothrombin (coagulation factor II) have been isolated and the composite DNA sequence has been determined. The complete prothrombin cDNA is 1,987 bp in length [excluding the poly(A) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 61...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.487
更新日期:1990-09-01 00:00:00
abstract::Haplotypes formed by polymorphisms (T-786C, rs2070744; a variable number of tandem repeats in intron 4, and Glu298Asp, rs1799983) of the eNOS gene were associated previously with gestational hypertension (GH) and preeclampsia (PE). However, no study has explored the Tag SNPs rs743506 and rs7830 in these disorders. The...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2012.1768
更新日期:2012-12-01 00:00:00
abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::The murine 18A2/mts1 and its human homolog h-mts1 (S100A4), encoding a Ca2+-binding protein belonging to the S-100 family, are associated with high invasive and metastatic potentials of murine tumors, human tumor cell lines in vitro, and human tumors growing as xenografts. The nm23 is a putative metastasis-suppressor ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.335
更新日期:1998-04-01 00:00:00
abstract::We evaluated the effects of administration of 1,25-dihydroxyvitamin D (1,25(OH)2D) during pregnancy on relieving adverse outcomes of preeclampsia and the pathologic and biochemical changes in reduction in uteroplacental perfusion (RUPP) model of rats. On day 1, 7, and 14 of pregnancy, rats in pregnant RUPP plus 1,25(O...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2885
更新日期:2016-02-01 00:00:00
abstract::The rat D2 dopamine receptor gene is transcribed from a TATA-less promoter that has an initiator-like sequence and several putative Sp1 binding sites. We previously reported that a negative modulator is located between nucleotides -116 and -76 (D2Neg-B) in this gene and that Sp1 as well as another unknown factor bind ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.471
更新日期:1998-05-01 00:00:00
abstract::The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans exposed to occupational and environmental agents. The MN test is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. An increased mutati...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0996
更新日期:2010-06-01 00:00:00
abstract::Epigenetic modifications, especially alteration in DNA methylation, are increasingly being recognized as a key factor in the pathogenesis of complex disorders, including atherosclerosis. However, there are limited data on the epigenetic changes in the coronary artery disease (CAD) patients. In the present study we eva...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0694
更新日期:2008-07-01 00:00:00
abstract::Non-Hodgkin lymphoma (NHL) is the most common hematologic malignancy worldwide. Interleukin-2 (IL-2) plays a key role in the proliferation of T cells and natural killer cells. It has been reported that polymorphisms in the IL-2 gene are associated with various cancers. The aim of this study was to examine the effect o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1603
更新日期:2012-07-01 00:00:00
abstract::In this study, we explored the genes genetically associated with lupus nephritis (LN), and their function by bioinformatics analysis. We collected genes potentially associated with LN from National Center for Biotechnology Information Center (NCBI-Gene) and Online Mendelian Inheritance in Man (OMIM) databases. The maj...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.4631
更新日期:2019-07-01 00:00:00
abstract::The aim of this work was to express a eukaryotic pre-protein in Escherichia coli so that it could be obtained intact, without cleavage, by bacterial leader peptidase. To this end, cDNA coding for honeybee prepromelittin was ligated to the 3' end of genes coding for truncated forms of either Protein A or beta-galactosi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.875
更新日期:1994-08-01 00:00:00
abstract::Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490150504666
更新日期:2001-01-01 00:00:00
abstract::We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that s...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.339
更新日期:1996-04-01 00:00:00
abstract::Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main tra...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3535
更新日期:2017-03-01 00:00:00
abstract::Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320308942
更新日期:2002-08-01 00:00:00
abstract::Cardiomyocytes are notoriously difficult to transfect using standard techniques unless viral vectors such as recombinant adenoviruses are used. Generation of recombinant adenoviruses is, however, a complex and time-consuming procedure and not possible for every DNA construct. We therefore optimized DNA/polylysine/aden...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.381
更新日期:2005-06-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.0546
更新日期:2007-05-01 00:00:00
abstract::Voltage-gated potassium channels play important functional roles in the development and maintenance of human lymphocyte functions. One such channel, known as the type n channel, has been well defined in human T cells and exhibits unique functional properties that distinguish it from other species of potassium channels...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.163
更新日期:1992-03-01 00:00:00
abstract::Homomeric and heteromeric interactions among cell-surface tyrosine kinase receptors belonging to the ErbB family lead to intracellular signaling cascades which are involved in cell activation, cytoskeletal interactions, and cellular transformation leading to neoplasia. Monoclonal antibodies which specifically bind to ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.1031
更新日期:1998-12-01 00:00:00
abstract:PURPOSE:This case-control study was conducted in order to evaluate the potential role of polymorphic genes encoding enzymes involved in estrogen biosynthesis (CYP19A1) and metabolism (GSTM1, GSTT1, and GSTP1), and their action in modulating individual susceptibility to breast cancer. METHODS:Genomic DNA was extracted ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1538
更新日期:2012-06-01 00:00:00
abstract::Uterine corpus endometrial carcinoma (UCEC) is one of the leading causes of death from gynecological cancer due to the high recurrence rate. A recent study indicated that molecular biomarkers can enhance the recurrence prediction power if they were integrated with clinical information. In this study, we attempted to i...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2019.5148
更新日期:2020-04-01 00:00:00
abstract::Genes involved in the production of nitric oxide (NO) have been suggested as genetic factors for migraine. It has been studied whether polymorphisms in the genes encoding for different types of NO synthase (NOS) could be involved in the liability to migraine; however, most studies yield negative results. The pentanucl...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1102
更新日期:2011-02-01 00:00:00
abstract::The abnormal function of O(6)-methylguanine-DNA methyltransferase (MGMT) is reported to be associated with the occurrence of various tumors and malignant tumor progression. However, little evidence is available to describe its role in esophageal carcinogenesis. To address this issue, we constructed a stable MGMT-silen...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1318
更新日期:2012-05-01 00:00:00
abstract::The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma prot...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.1063
更新日期:1994-11-01 00:00:00
abstract::Accumulating evidence has suggested that microRNAs play important roles in the development of hepatocellular carcinoma (HCC) and are involved in drug resistance. miR-21-5p was overexpressed in a variety of cancers and promoted the tumorigenesis; however, the function of miR-21-5p in HCC still remains unknown. In this ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4529
更新日期:2019-08-01 00:00:00
abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.613
更新日期:1998-07-01 00:00:00