Isolation of cDNA clones for 42 different Krüppel-related zinc finger proteins expressed in the human monoblast cell line U-937.

Abstract:

:To study the complexity and structural characteristics of zinc finger proteins expressed during human hematopoiesis and to isolate novel regulators of blood cell development, a degenerate oligonucleotide probe specific for a consensus zinc finger peptide domain was used to isolate 63 cDNA clones for Krüppel-related zinc finger genes from the human monoblast cell line U-937. By extensive nucleotide sequence and Northern blot analysis, these cDNA clones were found to originate from approximately 42 different genes (HZF 1-42) of which only 8 have previously been described. Northern blot analysis showed that a majority of these genes were expressed at comparable levels in U-937 and HeLa cells. The large number of individual genes represented among the 63 clones and their apparent non-cell-type-specific expression suggest that the majority of the Krüppel-related zinc finger genes are likely to be expressed in most human tissues. In contrast, some of the genes displayed a restricted expression pattern, indicating that they represent potential regulators of monocyte differentiation or proliferation. Detailed structural analysis of the first 12 cDNAs (HZF 1-10) and a partial characterization of HZF 11-42 revealed that a common feature of human Krüppel-related zinc finger proteins is the presence of tandem arrays of zinc fingers ranging in number from 3 to over 20 that are preferentially located in the carboxy-terminal regions of the proteins. In addition, several novel KRAB-containing zinc finger genes and a novel conserved sequence element were identified.

journal_name

DNA Cell Biol

journal_title

DNA and cell biology

authors

Abrink M,Aveskogh M,Hellman L

doi

10.1089/dna.1995.14.125

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

125-36

issue

2

eissn

1044-5498

issn

1557-7430

journal_volume

14

pub_type

杂志文章
  • Identification and Expression Analysis of the SWEET Gene Family from Poa pratensis Under Abiotic Stresses.

    abstract::The sugars will eventually be exported transporters (SWEET) gene family is a glycoprotein gene family that can regulate the transport of sugar in plants and plays an important role in plant growth and development, as well as in response to environmental stress. In this study, Kentucky bluegrass (cv. Baron) seedlings w...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2020.5418

    authors: Zhang R,Niu K,Ma H

    更新日期:2020-09-01 00:00:00

  • Satellite-tagged transcribing sequences in Bubalus bubalis genome undergo programmed modulation in meiocytes: possible implications for transcriptional inactivation.

    abstract::We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshybridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/104454901317095007

    authors: Chattopadhyay M,Gangadharan S,Kapur V,Azfer MA,Prakash B,Ali S

    更新日期:2001-09-01 00:00:00

  • Interleukin 2 gene polymorphisms are associated with non-Hodgkin lymphoma.

    abstract::Non-Hodgkin lymphoma (NHL) is the most common hematologic malignancy worldwide. Interleukin-2 (IL-2) plays a key role in the proliferation of T cells and natural killer cells. It has been reported that polymorphisms in the IL-2 gene are associated with various cancers. The aim of this study was to examine the effect o...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2011.1603

    authors: Song H,Chen L,Cha Z,Bai J

    更新日期:2012-07-01 00:00:00

  • Mouse HNF-3/fork head homolog-1-like gene: structure, chromosomal location, and expression in adult and embryonic kidney.

    abstract::Screening of a mouse kidney cDNA library with a HNF-3/fork head domain probe revealed cDNA Hfh-1L containing the highly conserved fork head DNA-binding domain. The Hfh1L cDNA shows 92.7% homology at the nucleic acid level with the fork head gene HFH-1 from rat. Southern blot analyses demonstrated that the Hfh-1L gene ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1998.17.679

    authors: Frank S,Zoll B

    更新日期:1998-08-01 00:00:00

  • Effects of plasmid DNA injection on cyclophosphamide-accelerated diabetes in NOD mice.

    abstract::Type 1 diabetes results in most cases from the destruction of insulin-secreting beta cells by the immune system. Several immunization methods based on administration of autoantigenic polypeptides such as insulin and glutamic acid decarboxylase (GAD) have been used to prevent autoimmune diabetes in the non-obese diabet...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/104454901300069022

    authors: Filippova M,Liu J,Escher A

    更新日期:2001-03-01 00:00:00

  • Differing effects of azithromycin and doxycycline on cytokines in cells from Chlamydia trachomatis-infected women.

    abstract::Chlamydial infection of the lower genital tract usually spreads to the upper genital tract and is then responsible for more serious consequences, such as infertility, ectopic pregnancy, pelvic pain, and pelvic inflammatory disease. Genital infection with Chlamydia trachomatis and the resulting cytokine response largel...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2011.1333

    authors: Srivastava P,Bhengraj AR,Jha HC,Vardhan H,Jha R,Singh LC,Salhan S,Mittal A

    更新日期:2012-03-01 00:00:00

  • COX-2 inhibitor, NS398, enhances Fas-mediated apoptosis via modulation of the PTEN-Akt pathway in human gastric carcinoma cell lines.

    abstract::A variety of human cancer cells are resistant to Fas ligand and anti-Fas antibody induced apoptosis. Previously, we reported that human gastric carcinoma cell lines were resistant to the anti-Fas antibody, CH-11, without interferon-gamma pretreatment in vitro. Cyclooxygenase (COX)-2 is known to be expressed in many hu...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2005.24.141

    authors: Honjo S,Osaki M,Ardyanto TD,Hiramatsu T,Maeta N,Ito H

    更新日期:2005-03-01 00:00:00

  • Cytology of terminally differentiated Epulopiscium mother cells.

    abstract::Epulopiscium sp. type B, a member of the Firmicutes, is a large (up to 300 microm), cigar-shaped bacterial symbiont of surgeonfish that propagates itself by forming multiple intracellular offspring. This unusual form of reproduction is an apparent modification of a developmental program used by some Firmicutes to prod...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2008.0801

    authors: Ward RJ,Clements KD,Choat JH,Angert ER

    更新日期:2009-02-01 00:00:00

  • Functional dissection of the HNF-1alpha transcription factor: a study on nuclear localization and transcriptional activation.

    abstract::Hepatocyte nuclear factor-1alpha (HNF-1alpha) is a homeodomain-containing transcription factor regulating the expression of liver and pancreas-specific genes. Mutations in the HNF-1alpha-encoding gene TCF1 cause maturity-onset diabetes of the young, type 3 (MODY3). These mutations may affect nuclear import or reduce t...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2005.24.661

    authors: Bjørkhaug L,Bratland A,Njølstad PR,Molven A

    更新日期:2005-11-01 00:00:00

  • Expression of metastasis-associated genes h-mts1 (S100A4) and nm23 in carcinoma of breast is related to disease progression.

    abstract::The murine 18A2/mts1 and its human homolog h-mts1 (S100A4), encoding a Ca2+-binding protein belonging to the S-100 family, are associated with high invasive and metastatic potentials of murine tumors, human tumor cell lines in vitro, and human tumors growing as xenografts. The nm23 is a putative metastasis-suppressor ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1998.17.335

    authors: Albertazzi E,Cajone F,Leone BE,Naguib RN,Lakshmi MS,Sherbet GV

    更新日期:1998-04-01 00:00:00

  • Escaping the cut by restriction enzymes through single-strand self-annealing of host-edited 12-bp and longer synthetic palindromes.

    abstract::Palindromati, the massive host-edited synthetic palindromic contamination found in GenBank, is illustrated and exemplified. Millions of contaminated sequences with portions or tandems of such portions derived from the ZAP adaptor or related linkers are shown (1) by the 12-bp sequence reported elsewhere, exon Xb, 5' CC...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2011.1339

    authors: Castro-Chavez F

    更新日期:2012-02-01 00:00:00

  • Expression and export in Escherichia coli of fusion proteins containing carboxy-terminally located honeybee prepromelittin.

    abstract::The aim of this work was to express a eukaryotic pre-protein in Escherichia coli so that it could be obtained intact, without cleavage, by bacterial leader peptidase. To this end, cDNA coding for honeybee prepromelittin was ligated to the 3' end of genes coding for truncated forms of either Protein A or beta-galactosi...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1994.13.875

    authors: He M,Liu H,Austen B

    更新日期:1994-08-01 00:00:00

  • Biodynamic modeling and simulation of multistage cell mutations.

    abstract::The aim of this study is to present a mathematical computer simulation model for multistage carcinogenesis. The population genetic model is developed based on the reaction diffusion, logistic behavior, and Hollings Type II interactions between normal, benign, and premalignant cells. The simple form of the Fisher-Halda...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2004.23.625

    authors: Ahangar R,Ali N,Iqbal K,Altmayer K

    更新日期:2004-10-01 00:00:00

  • Enhancer, repressor, and promoter specificities combine to regulate the rat alpha-fetoprotein gene.

    abstract::The upstream transcription control region of the rat alpha-fetoprotein (AFP) gene was analyzed using transient expression of CAT genes in HepG2 cells which express the gene; H4C3 cells which repress the AFP gene but express the albumin gene; and four nonexpressing cell lines. Deletion analysis based on the DNA sequenc...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1991.10.525

    authors: Wen P,Groupp ER,Buzard G,Crawford N,Locker J

    更新日期:1991-09-01 00:00:00

  • Genomic organization of the genes for human and mouse CC chemokine LEC.

    abstract::Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed in the liver. We report here the structures of the human and mouse genes for LEC. The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8, HCC-2/...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/104454999315330

    authors: Fukuda S,Hanano Y,Iio M,Miura R,Yoshie O,Nomiyama H

    更新日期:1999-04-01 00:00:00

  • How does cellular senescence prevent cancer?

    abstract::It is widely believed that cellular senescence is a tumor suppressor mechanism; however, it has not been understood why it is advantageous for organisms to retain mutant cells is a postmitotic state rather than simply eliminating them by apoptosis. It has recently been proposed that the primary role of cellular senesc...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2006.25.69

    authors: Lynch MD

    更新日期:2006-02-01 00:00:00

  • High-copy expression vector based on amplification-promoting sequences.

    abstract::We describe a new vector system that allows efficient expression of heterologous proteins in transformed mouse L fibroblasts. This is due to its persistence at high copy numbers, achieved by a 370-bp amplification promoting element (muNTS1) derived from the nontranscribed spacer of murine rDNA. Copy number determinati...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1994.13.437

    authors: Hemann C,Gärtner E,Weidle UH,Grummt F

    更新日期:1994-04-01 00:00:00

  • Sp family transcription factors regulate expression of rat D2 dopamine receptor gene.

    abstract::The rat D2 dopamine receptor gene is transcribed from a TATA-less promoter that has an initiator-like sequence and several putative Sp1 binding sites. We previously reported that a negative modulator is located between nucleotides -116 and -76 (D2Neg-B) in this gene and that Sp1 as well as another unknown factor bind ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1998.17.471

    authors: Yajima S,Lee SH,Minowa T,Mouradian MM

    更新日期:1998-05-01 00:00:00

  • Regenerative medicine for diseases of the head and neck: principles of in vivo regeneration.

    abstract::The application of endogenous regeneration in regenerative medicine is based on the concept of inducing regeneration of damaged or lost tissues from residual tissues in situ. Therefore, endogenous regeneration is also termed in vivo regeneration as opposed to mechanisms of ex vivo regeneration which are applied, for e...

    journal_title:DNA and cell biology

    pub_type: 杂志文章,评审

    doi:10.1089/104454903322405464

    authors: Löwenheim H

    更新日期:2003-09-01 00:00:00

  • Microarray analysis of butyrate regulated genes in colonic epithelial cells.

    abstract::Butyrate is a naturally occurring product of colonic microbial fermentation of dietary carbohydrates that escape hydrolysis in the small intestine. Butyrate plays a significant role in the maintenance of colonic tissue homeostasis by regulating the expression of genes associated with the processes of proliferation, di...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2006.25.49

    authors: Daly K,Shirazi-Beechey SP

    更新日期:2006-01-01 00:00:00

  • Cloning and characterization of buffalo NANOG gene: alternative transcription start sites, splicing, and polyadenylation in embryonic stem cell-like cells.

    abstract::NANOG is a critical homeodomain transcription factor responsible for maintaining embryonic stem cell (ESC) self-renewal and pluripotency. In the present study, we isolated, sequenced, and characterized the NANOG gene in buffalo ESC-like cells. Here, we demonstrated that NANOG mRNA is expressed as multiple isoforms and...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2011.1410

    authors: Singh N,Sharma R,George A,Singla SK,Palta P,Manik R,Chauhan MS,Singh D

    更新日期:2012-05-01 00:00:00

  • The Wilms' tumor gene 1 (WT1) induces expression of the N-myc downstream regulated gene 2 (NDRG2).

    abstract::The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2007.0586

    authors: Svensson E,Vidovic K,Olofsson T,Vallon-Christersson J,Borg A,Gullberg U

    更新日期:2007-08-01 00:00:00

  • Investigation of serotonin transporter gene promoter (5-HTTLPR) and intron 2 (variable number of tandem repeats) polymorphisms with suicidal behavior in a Turkish population.

    abstract::The serotonin (5-hydroxytryptamine) transporter (5HTt) gene has been reported to be associated with suicidal behavior. In this study, we have investigated the 5' promoter region (i.e., 5HTt gene-linked polymorphic region [5-HTTLPR]) and a 17-base pair variable number of tandem repeats polymorphism in the 5HTt gene for...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2010.1031

    authors: Akar T,Sayin A,Bakkaloğlu Z,Cabuk DK,Küçükyildirim S,Demirel B,Candansayar S,Ozsoy ED,Mergen H

    更新日期:2010-08-01 00:00:00

  • Characterization of a novel human anti-HIV-1 gp41 IgM monoclonal antibody designated clone 37.

    abstract::Human monoclonal antibodies (HuMAbs) demonstrate great potential for passive immunotherapy against HIV-1. The gp41 transmembrane envelope glycoprotein of HIV has an important role in the pathogenicity of AIDS and importantly displays considerably less hypervariability than the gp120 surface envelope HIV glycoprotein, ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2004.23.836

    authors: Cao C,Bai Y,Holloway MJ,Edgeworth RL,Jackson EA Jr,Cotropia J,Ugen KE

    更新日期:2004-12-01 00:00:00

  • miRNA-106a Promotes Breast Cancer Cell Proliferation, Clonogenicity, Migration, and Invasion Through Inhibiting Apoptosis and Chemosensitivity.

    abstract::To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated w...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2018.4282

    authors: You F,Luan H,Sun D,Cui T,Ding P,Tang H,Sun D

    更新日期:2019-02-01 00:00:00

  • An NFIL-6 sequence near the transcriptional initiation site is necessary for the lipopolysaccharide induction of murine interleukin-1 beta.

    abstract::We have analyzed the interleukin-1 beta (IL-1 beta) promoter region near the cap site. Specific DNA sequences required for lipopolysaccharide (LPS) induction within this region were identified using transfection of reporter plasmids that contained portions of the proximal IL-1 beta 5'-flanking sequence. An LPS-respons...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1994.13.561

    authors: Godambe SA,Chaplin DD,Takova T,Bellone CJ

    更新日期:1994-06-01 00:00:00

  • Characterization of murine middle repetitive DNA.

    abstract::We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracister...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1993.12.319

    authors: Adiletta DC,Elliott RW,Woodworth ME

    更新日期:1993-05-01 00:00:00

  • Novel immunoblotting monoclonal antibodies against human and rat CD36/fat used to identify an isoform of CD36 in rat muscle.

    abstract::CD36, a surface membrane glycoprotein, functions as a class B scavenger receptor that binds to several distinct ligands. Included among these is oxidized low-density lipoprotein (Ox-LDL), a major trigger of atherosclerotic lesions, and the levels of CD36 activity and Ox-LDL uptake may have an impact on coronary artery...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2006.25.302

    authors: Thorne RF,Zhang X,Song C,Jin B,Burns GF

    更新日期:2006-05-01 00:00:00

  • Alternate arrangement of PpL B3 domain and SpA D domain creates synergistic double-site binding to VH3 and Vkappa regions of fab.

    abstract::In our previous study, a kind of novel hybrid immunoglobulin (Ig)-binding proteins (IBPs) was obtained with the characteristic structure of alternately arranged Finegoldia magna (formerly Peptostreptococcus magnus) protein L (P. magnus protein L, PpL) B3 domain (B3) and Staphylococcal protein A (SpA) D domain (D). In ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.2007.0708

    authors: Jiang SH,Wang JF,Xu R,Liu YJ,Wang XN,Cao J,Zhao P,Shen YJ,Yang T,Yang H,Jia JA,Chen QL,Pan W

    更新日期:2008-08-01 00:00:00

  • Complete structure and expression of the rat alpha B-crystallin gene.

    abstract::alpha B-Crystallin, a member of the small heat shock family of proteins, is synthesized as a component of various developmental programs, in response to stress and in a number of pathological states. We have determined the complete structure of the alpha B-crystallin gene (6,806 bp encompassing 2,299 bp upstream from ...

    journal_title:DNA and cell biology

    pub_type: 杂志文章

    doi:10.1089/dna.1994.13.651

    authors: Srinivasan AN,Bhat SP

    更新日期:1994-06-01 00:00:00