Abstract:
:We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracisternal A-particle (IAP) elements, a second clone (pMR89) has partial homology with a sequence in the 3' untranslated region of the human fibulin gene, while two clones (pMR6, pMR66) are new that have no homology to any reported DNA sequence. Each clone hybridizes to one or two discrete RNA transcripts from one or more tissues of the mouse. Clone pMR66 detected restriction fragment length polymorphisms (RFLPs) at three loci in genomic mouse DNA, defining loci at the distal end of chromosome 5 and the proximal end of chromosome 7. The third locus is unmapped. This study demonstrates that cloned repeats from a repetitive DNA library are a potential source of genetic markers.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Adiletta DC,Elliott RW,Woodworth MEdoi
10.1089/dna.1993.12.319subject
Has Abstractpub_date
1993-05-01 00:00:00pages
319-27issue
4eissn
1044-5498issn
1557-7430journal_volume
12pub_type
杂志文章abstract::Microsomal transfer protein (MTP), a lipid transfer protein localized in the endoplasmic reticulum of hepatocytes and enterocytes, plays an important role in the development of nonalcoholic fatty liver disease (NAFLD). Many existing studies have demonstrated that a common polymorphism (-493G>T, rs1800591 G>T) in the M...
journal_title:DNA and cell biology
pub_type: 杂志文章,meta分析
doi:10.1089/dna.2013.2238
更新日期:2014-06-01 00:00:00
abstract::A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence com...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.437
更新日期:1990-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2014.2452
更新日期:2014-08-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4584
更新日期:2019-06-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2656
更新日期:2015-05-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.275
更新日期:1994-03-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902760330219
更新日期:2002-09-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2014.2554
更新日期:2014-12-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1603
更新日期:2012-07-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.195
更新日期:1995-03-01 00:00:00
abstract::Epigenetic modifications, especially alteration in DNA methylation, are increasingly being recognized as a key factor in the pathogenesis of complex disorders, including atherosclerosis. However, there are limited data on the epigenetic changes in the coronary artery disease (CAD) patients. In the present study we eva...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0694
更新日期:2008-07-01 00:00:00
abstract::Increasing evidence has announced the emerging roles of long noncoding RNAs (lncRNAs) in modulating bone homeostasis due to their potential regulating effects on bone-related cells' proliferation, migration, differentiation and apoptosis. Thus, lncRNAs have been considered as a promising gene tool to facilitate the bo...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2020.5391
更新日期:2020-06-01 00:00:00
abstract::Tumor cells express lower levels of manganese superoxide dismutase (MnSOD) than their normal counterparts. Enforced expression of MnSOD reverses the malignant phenotype of many transformed cells, suggesting that SOD2 is a tumor suppressor. The SOD2 gene contains a large CpG island spanning > 3.5 kb that starts near th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315051
更新日期:1999-08-01 00:00:00
abstract::It is widely believed that cellular senescence is a tumor suppressor mechanism; however, it has not been understood why it is advantageous for organisms to retain mutant cells is a postmitotic state rather than simply eliminating them by apoptosis. It has recently been proposed that the primary role of cellular senesc...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.69
更新日期:2006-02-01 00:00:00
abstract::The distribution and evolutionary pattern of the conserved microsatellite repeat sequences (CA)n, (TGG)6, and (GGAT)4 were studied to determine the divergence time and phylogenetic position of the water buffalo, Bubalus bubalis. The mean allelic frequencies of these repeat loci showed a high level of heterozygosity am...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315231
更新日期:1999-06-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD polypeptide originally purified from media conditioned by interleukin-1 beta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the normal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK cells, 49F, we found that lipopolysacchar...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.883
更新日期:1994-09-01 00:00:00
abstract::In IL-5 transgenic mice (C3H/HeN-TgN(IL-5)-Imeg), in which 50% of peripheral blood leukocytes are eosinophils, the development of infection by Leishmania amazonensis was clearly suppressed. To determine mechanistically how this protozoan parasite is killed, we performed in vitro killing experiments. Either IL-4 or IFN...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/1044549041474805
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0714
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journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.661
更新日期:2005-11-01 00:00:00
abstract::The aim of this work was to express a eukaryotic pre-protein in Escherichia coli so that it could be obtained intact, without cleavage, by bacterial leader peptidase. To this end, cDNA coding for honeybee prepromelittin was ligated to the 3' end of genes coding for truncated forms of either Protein A or beta-galactosi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.875
更新日期:1994-08-01 00:00:00
abstract::Intracranial aneurysms (IAs) and potential IA rupture are one of the direct causes of permanent brain damage and mortality. Interestingly, the major risk factors of IA development, including hemodynamic stress, hypertension, smoking, and genetic predispositions, are closely associated with a proinflammatory immune sta...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.3105
更新日期:2016-02-01 00:00:00
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journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4464
更新日期:2019-10-01 00:00:00
abstract::The impact of chromatin topology on the DNA synthetic process was studied in the human squamous-cell carcinoma cell line SQ-20B. A 1-h exposure < or = 10 microM VP16 produced an increase in DNA supercoil tension, measured by recording laser light scatter from salt-extracted nuclei. This change was precisely paralleled...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.613
更新日期:1998-07-01 00:00:00
abstract::Haplotypes formed by polymorphisms (T-786C, rs2070744; a variable number of tandem repeats in intron 4, and Glu298Asp, rs1799983) of the eNOS gene were associated previously with gestational hypertension (GH) and preeclampsia (PE). However, no study has explored the Tag SNPs rs743506 and rs7830 in these disorders. The...
journal_title:DNA and cell biology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
abstract::Six identical cDNA clones corresponding to an RNA of 1685 nucleotides that is enriched in mouse sperm compared with testis were isolated from a mouse testis cDNA library. The sequence of these clones corresponds to the 16S mitochondrial RNA plus an inverted repeat of 120 bp covalently joined to the 5' end of the RNA. ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900439809
更新日期:2000-09-01 00:00:00
abstract::CONSTANS (CO)/CONSTANS-like (COL) genes that have been studied in annual model plants such as Arabidopsis thaliana and Oryza sativa play key roles in the photoperiodic flowering pathway. Moso bamboo is a perennial plant characterized by a long vegetative stage and flowers synchronously followed by widespread death. Ho...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4611
更新日期:2019-07-01 00:00:00