Abstract:
:A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence comprises the known amino acid sequence of SAP with an amino terminal representing a putative signal sequence; at the carboxyl terminus the sequence contains an additional lysine residue. Present experimental data do not distinguish between two potential SAP precursor molecules, each starting with a methionine residue and differing by 10 amino acid residues in the leader peptide. Comparative Northern analysis reveals a SAP-specific mRNA of 700 bp, which lacks RNA from bovine testis as well as from seminal vesicle tissue of a bull calf; hence, expression of the SAP gene appears to be under androgen and/or developmental control. Southern analysis indicates that one gene appears to specify SAP. SAP-like DNA sequences were detected in ovine and porcine genomic DNA.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Wagner S,Freudenstein J,Scheit KHdoi
10.1089/dna.1990.9.437subject
Has Abstractpub_date
1990-07-01 00:00:00pages
437-42issue
6eissn
1044-5498issn
1557-7430journal_volume
9pub_type
杂志文章abstract::Zidovudine (AZT) is widely used in the treatment of human immunodeficiency virus (HIV) infection because of its ability to act as a false substrate for the viral reverse transcriptase. AZT in the clinical setting has also been shown to produce significant hematologic and other toxicity, even though prior reports in th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1996.15.363
更新日期:1996-05-01 00:00:00
abstract::We previously showed that the human AKNA gene encodes an AT-hook transcription factor that regulates the expression of costimulatory cell surface molecules on lymphocytes. However, AKNA cDNA probes hybridize with multiple transcripts, suggesting either the existence of other homologous genes or a complex regulation op...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.325
更新日期:2005-05-01 00:00:00
abstract::Recently, we demonstrated that the DAN gene product contains a growth- and/or a tumor-suppressive activity in vitro. In the present work, using a yeast two-hybrid system, we searched for cellular proteins that can associate with the DAN gene product. A cDNA clone, termed DA41, was initially isolated from an adult rat ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1997.16.985
更新日期:1997-08-01 00:00:00
abstract::We have cloned a putative yeast homolog of the gene encoding the human Tat-binding protein, TBP-1. The gene termed TBPY encodes a 45,243-dalton protein displaying a heptad repeat of hydrophobic amino acids reminiscent of a leucine zipper. Secondary structure predictions suggest the possibility of formation of an amphi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1992.11.579
更新日期:1992-10-01 00:00:00
abstract::Head and neck cancers (head and neck squamous cell carcinomas [HNSCC]) are a heterogeneous group of neoplasms with varying presenting symptoms, treatment, and expected outcome. There is a need to find an effective way of its treatment at the molecular level. Thus, we should identify the mechanism of cancer cell respon...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2011.1325
更新日期:2012-03-01 00:00:00
abstract::Exposure to microgravity is supposed to affect almost all biological systems, and we speculated that microgravity is potentially involved in autophagy regulation. A clinostat was used to simulate microgravity, and HEK293 cells that stably express GFP-LC3 were used for sensitive monitoring of autophagy induction. The c...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2013.2089
更新日期:2014-03-01 00:00:00
abstract::It is widely believed that cellular senescence is a tumor suppressor mechanism; however, it has not been understood why it is advantageous for organisms to retain mutant cells is a postmitotic state rather than simply eliminating them by apoptosis. It has recently been proposed that the primary role of cellular senesc...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.69
更新日期:2006-02-01 00:00:00
abstract::Promoter elements located up to 2 kb upstream of the apolipoprotein A-I (apoA-I) gene are necessary for apoA-I expression in liver and intestine cells in tissue culture. In transgenic mice, a distal enhancer located between the apoA-IV and apoC-III genes is additionally necessary for tissue-specific expression of apoA...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315493
更新日期:1999-02-01 00:00:00
abstract::Effective and efficient efferocytosis of dead cells and associated cellular debris are critical to tissue homeostasis and healing of injured tissues. This important task was previously thought to be restricted to professional phagocytes (PPs). However, accumulating evidence has revealed another type of phagocyte, the ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2020.5647
更新日期:2021-01-13 00:00:00
abstract::The G0S19 genes are members of the "small inducible" family of genes, which have similar exon-intron organizations and encode secreted proteins with similar dispositions of cysteine and proline residues. G0S19-1 mRNA is increased shortly after the addition of lectin or cycloheximide to cultured human blood mononuclear...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.589
更新日期:1990-10-01 00:00:00
abstract::At supramolecular resolution, DNA synthesis begins at preferred replication origins in the chromosomes of metazoan cells. To characterize one of these origins in detail, the initiation of replication was examined in the HeLa c-myc origin. Polymerase chain reaction (PCR) amplification of size-fractionated nascent chrom...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.885
更新日期:1998-10-01 00:00:00
abstract::Growth regulation of terminal differentiation was studied by analysis of the promoter of the transcription factor D-site binding protein (DBP) using nuclear extracts from rat liver undergoing regeneration or from normal liver early in development. Four proximal binding sites for nuclear proteins were identified, three...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1994.13.693
更新日期:1994-07-01 00:00:00
abstract::The role of p53 in mediating nitric oxide (NO)-induced cell death remains uncertain. The exogenous NO donor S-nitrosoglutathione (GSNO) produced a concentration-dependent reduction in cell viability in embryonic chick cardiomyocytes in culture. Western blotting and immunocytochemistry for p53 showed that p53 was incre...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0576
更新日期:2007-07-01 00:00:00
abstract::Extracellular vesicles classified as exosomes, microvesicles, or apoptotic bodies based on size are shed from most cells under normal as well as pathological conditions. They are released into the surrounding milieu, including plasma, urine, saliva, and tissues. Exosomes are highly enriched in microRNAs (miRs), which ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2015.2884
更新日期:2015-07-01 00:00:00
abstract::Mutations in the mitochondrial genome (mtDNA) are associated with different types of cancer, specifically colorectal cancer (CRC). However, few studies have been performed on precancerous lesions, such as ulcerative colitis (UC) lesions and adenomatous polyps (AP). The aim of this study was to identify mtDNA mutations...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1186
更新日期:2011-11-01 00:00:00
abstract::Most of the signal pathways involved in ultraviolet (UV)-induced skin carcinogenesis are thought to originate at plasma membrane receptors. However, UVA-induced signal transduction to downstream ribosomal protein S6 kinases, p70(S6K) and p90(RSK), is not well understood. In this report, we show that UVA stimulation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753438589
更新日期:2001-12-01 00:00:00
abstract::In the rat, the growth hormone receptor (GH-R) gene generates two transcripts, one encoding the transmembrane GH-R, and a shorter one encoding the GH-binding protein (GH-BP). These transcripts exhibit a high degree of heterogeneity in their 5'-untranslated regions (5'-UTRs). Some of the exons encoding these 5'-UTR var...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1995.14.195
更新日期:1995-03-01 00:00:00
abstract::In our previous study, a kind of novel hybrid immunoglobulin (Ig)-binding proteins (IBPs) was obtained with the characteristic structure of alternately arranged Finegoldia magna (formerly Peptostreptococcus magnus) protein L (P. magnus protein L, PpL) B3 domain (B3) and Staphylococcal protein A (SpA) D domain (D). In ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2007.0708
更新日期:2008-08-01 00:00:00
abstract::FOXO3 is a member of the Forkhead box class O (FOXO) transcription factor family that modulates the transcription of target genes in a variety of cellular functions in lower animals to mammals. It has been implicated in the attenuation of insulin-signaling pathways, including having a potential role in lifespan contro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2010.1193
更新日期:2011-07-01 00:00:00
abstract::Previous studies showed that when growth hormone-releasing hormone (GHRH) was administered to either pregnant rats or pigs as a plasmid-mediated therapy, pituitary weight, somatotroph and lactotroph numbers, and postnatal growth rate of the offspring increased. To determine if these responses resulted from direct effe...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2006.25.429
更新日期:2006-08-01 00:00:00
abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320908441
更新日期:2002-11-01 00:00:00
abstract::RUNX2 is a member of the RUNX family of transcription factors, also containing the RUNX1 and RUNX3 proteins. These factors control the expression of genes essential for proper development in many cell lineages. RUNX2 plays a crucial role in the proliferation and differentiation of osteoblasts, required for bone format...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2014.2688
更新日期:2015-05-01 00:00:00
abstract::The Egr-1 (zfp-6) gene encodes a zinc-finger-containing nuclear protein that is rapidly and transiently induced in quiescent cells treated with mitogens. We have constructed baculovirus vectors that synthesize mouse Egr-1 protein initiating at two putative ATG start sites. The ATG site producing the larger protein (Mr...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1991.10.61
更新日期:1991-01-01 00:00:00
abstract::Temporal coordination of meiosis with spermatid morphogenesis is crucial for successful generation of mature sperm cells. We identified a recessive male sterile Drosophila melanogaster mutant, mitoshell, in which events of spermatid morphogenesis are initiated too early, before meiotic onset. Premature mitochondrial a...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2009.0989
更新日期:2010-09-01 00:00:00
abstract::The computer program, SIGSEQ2, was used to select heterologous signal peptides from a catalog of published sequences to express the echistatin gene in insect (Sf9) cells. S-values for each amino acid were determined to select empirically the site of cleavage between the signal peptide and mature echistatin. Five gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1990.9.453
更新日期:1990-07-01 00:00:00
abstract::Aberrant neutrophil (PMN) infiltration of the intestinal mucosa is a hallmark of inflammatory bowel diseases, including Crohn's disease and ulcerative colitis. While the genotoxic function of PMNs and its implications in carcinogenesis have been primarily associated with oxidative stress, recent work by Butin-Israeli ...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/dna.2019.4842
更新日期:2019-08-01 00:00:00
abstract::Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main tra...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2016.3535
更新日期:2017-03-01 00:00:00
abstract::Xenotransplantation from pigs to human beings is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal (a Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the eli...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2005.24.235
更新日期:2005-04-01 00:00:00
abstract::Accumulating evidence has suggested that microRNAs play important roles in the development of hepatocellular carcinoma (HCC) and are involved in drug resistance. miR-21-5p was overexpressed in a variety of cancers and promoted the tumorigenesis; however, the function of miR-21-5p in HCC still remains unknown. In this ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.2018.4529
更新日期:2019-08-01 00:00:00
abstract::Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490150504666
更新日期:2001-01-01 00:00:00