Abstract:
:The Egr-1 (zfp-6) gene encodes a zinc-finger-containing nuclear protein that is rapidly and transiently induced in quiescent cells treated with mitogens. We have constructed baculovirus vectors that synthesize mouse Egr-1 protein initiating at two putative ATG start sites. The ATG site producing the larger protein (Mr, 80,000) is similar, if not identical, to Egr-1 synthesized by serum-stimulated quiescent mouse fibroblasts, thus identifying the likely site for translation. The protein synthesized by the insect cells is active as assayed by its ability to bind to a specific DNA sequence that has been identified as an Egr-1 binding site. The insect cell system will allow further studies of the structure and function of the Egr-1 product, a protein that appears to be an important "master switch" for other genes.
journal_name
DNA Cell Bioljournal_title
DNA and cell biologyauthors
Ragona G,Edwards SA,Mercola DA,Adamson ED,Calogero Adoi
10.1089/dna.1991.10.61subject
Has Abstractpub_date
1991-01-01 00:00:00pages
61-6issue
1eissn
1044-5498issn
1557-7430journal_volume
10pub_type
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